Anatomy
aleenab
LabReport18Shell.pptxLab Report 18: Blood Typing with Simulated Blood
Name:
Date:
Adapted from jtcc.edu
Lab Materials
Online access
Computer (with PowerPoint software)
Camera (could be your phone)
Carolina Lab Manual – Blood typing with simulated blood
Kit – Blood typing with simulated blood
Personal protective equipment:
Goggles
Gloves
Lab apron
Safety Instructions:
Students must abide by the safety instructions in the Biology Lab Safety Contract
Safety Equipment & Instructions
Adapted from jtcc.edu
Lab Introduction
We have antibodies in our body to protect us from foreign substances, like viruses. They are proteins secreted by B cells (a white blood cell).
We can also use antibodies in the lab as a tool. Antibodies bind to specific substances, and if there’s enough of that substance, they will form large, clumps, called agglutination. This is NOT the same as a blood clot. A solution with antibodies is called anti-serum. It’s like a very specific fishing pole that will catch a specific fish, or in this case, a lot of fish.
Agglutination tells us that something that we normally cannot see (because it is too small) is present. Agglutination creates a large visible mass.
In this lab, we talk about both kinds of antibodies. A person with type B blood will have anti-A antibodies in her blood to protect her from foreign blood; but we can also have a tube of anti-A antibodies in the lab that we use to detect type A blood; we would call that tube anti-A anti-serum.
We will talk more about antigens later, but for now, let’s think of antigens as the things on red blood cells that antibodies can bind.
Adapted from jtcc.edu
General Lab Instructions
Readable Labels
Typed (not handwritten)
Use appropriate font size (24 or 26)
Reading frame is left-to-right, with horizontal, right-side-up textboxes
Use appropriate line weight for arrows, brackets, etc (4.5 or 6); do not use block arrows (please & thank you)
Contrasting Colors
Arrows and brackets should be of a high contrast color that is easily seen against the background.
Labels on the Slide, not off to the side; use the neutral spaces for textboxes.
Arrow in the Middle of the structure, not between structures
Photos should be sized proportionally
Use Lock Aspect Ratio
Resize using the corners, not the sides
No Criss-Cross
Arrows should not overlap
Photos from the Google Album (where applicable) should be oriented as they are in the album.
The completed lab needs to be submitted in Canvas upon completion in PowerPoint format (pptx) or as a pdf. If you are using a different program, you can Save As or Export As pptx or pdf file from the File Menu.
Adapted from jtcc.edu
4
Specific Lab Instructions
Take the time to read over the background information in the Carolina Lab Manual.
Take the time to make sure you understand each step in the instructions in the Carolina Lab Manual.
You may find it helpful to highlight each specific step.
You may find it helpful to take a few notes.
Take nice, clear, well-lit photos. Instagram-worthy! You want the agglutination (or lack of) to be visible in the photo. You may want to stand next to a sunny window or just make sure you use the flash on your phone camera.
Add your photos so that they are right-side up (well A is upper left, well B is lower left, well Rh is on the right).
Adapted from jtcc.edu
Table 1. Potential Testing Results
If you are testing patient blood types in the lab, you are using anti-sera (singular form = anti-serum, a solution that contains antibodies that you are using as a tool). Agglutination means that the antigen has been detected in the patient’s blood. For each of the given blood types, give the expected result when the blood is mixed with each antiserum. Type YES to indicate that agglutination occurs with the patient, and NO to indicate lack of agglutination.
Note – this is NOT the same chart that is on page 4 of the handout. Use critical thinking.
| Blood type | Anti-A | Anti-B | Anti-Rh |
| A+ | |||
| A- | |||
| B+ | |||
| B- | |||
| AB+ | |||
| AB- | |||
| O+ | |||
| O- |
Adapted from jtcc.edu
Table 2. Blood Donor
For each of the blood types, list types that would be expected to serve as donors and as recipients.
| Blood type | Could donate to: | Could receive from: |
| A+ | ||
| A- | ||
| B+ | ||
| B- | ||
| AB+ | ||
| AB- | ||
| O+ | ||
| O- |
Adapted from jtcc.edu
Photo 3. Patient Sample 1
Insert the photo from Activity 1, step 8, sample 1.
Adapted from jtcc.edu
Photo 4. Patient Sample 2
Insert the photo from Activity 1, step 8, sample 2.
Adapted from jtcc.edu
Photo 5. Patient Sample 3
Insert the photo from Activity 1, step 8, sample 3.
Adapted from jtcc.edu
Photo 6. Patient Sample 4
Insert the photo from Activity 1, step 8, sample 4.
Adapted from jtcc.edu
Table 7. Data Table 1
Fill in the table with the results of your tests.
In the first three columns, type YES if agglutination occurred, type NO if it did not occur.
Enter the blood type in the last column.
| Sample | Anti-A | Anti-B | Anti-Rh | ABO/Rh Blood Type |
| 1 | ||||
| 2 | ||||
| 3 | ||||
| 4 |
Adapted from jtcc.edu
Photo 8. Eldon Card (extra credit)
This activity is optional, for extra credit.
Insert the photo of your Eldon Card results.
What is your blood type?
Answer:
Adapted from jtcc.edu
