biology

Care Hope College

Microbiology-Lab Exam 1 to 7

Instructor: Dr. Gesner Cesar.

Name: _____________________________________ Date:_______________

Part A

1-The different steps Acid-fast staining. How do you recognize a non acid fast bacteria throughout the staining process?

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2-Matching Questions

Please match the numbers to the letters.

1. Gram-negative bacteria

2. Chlorine

3. Ozone

4. Chemical that preserves food.

5. Sterilizing agent

6. Preserve food by slowing the metabolic processes of foodborne microbes?

A. Freezing ___________________

B. Disinfection of water______________________

C. Oxidizing agents_____________________

D. Ethylene oxide_____________________

E. sodium nitrite.______________________

F. most resistant to chemical biocides_____________

3-Define the following key terms related to microbial control and if possible mention the chemicals used for each process:

a) Sterilization, b) disinfection, c) antisepsis, d)sanitization, e)bacteriostasis , f)asepsis.

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4- Establish the difference between Pasteurization and Autoclave and display their use in the Medical field.

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5- Difference between Generalized Transduction and specialized Transduction; ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

6-Difference between plasmid, transposons and bacteriophage.

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7-Summarize the Transduction by a bacteriophage

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8-Label the following bacterial conjugation

9-Difference between vertical gene transfer and Horizontal gene transfer ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

10-Label the Nitrogenous base-the sugar –the phosphate

11-Use your book to label the flow of genetic information in these bacteria

12-Complete the Griffith’s experiment chart demonstrating genetic transformation

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