Lab worksheet

Lab 9 – How does CNABlood agar select for bacteria and differentiate between bacteria

Submit to Lab 9 dropbox as Lab9-week9_LastName_FirstInital Due Friday Mon Aug 17– 10am.

Name____________________________ Date________________________

Section__________________________

Title:

Purpose:

Methods: Use of Columbia CNAblood agar to determine the virulence of potential pathogens

https://www.youtube.com/watch?v=ac2MX26sk5c

Explains the visual component you see on the agar.

https://www.youtube.com/watch?v=u0uoyEgLXVg&t

May have to copy and paste this link

There are examples of the hemolytic in the first 2:30 of the video

If you wait the blood will break down more – read the plates after 18 hours of incubation

Reaction

Three different types of bacteria were inoculated on CNABlood Agar plates

Streptococcus pyogenes, Streptococcus pneumonia and Streptococcus epidermidis ( Enterococcus facecalis)

Talk about the good examples and not the exceptions

Results

If gram was completed what would you see on your microscope – What shape – What color? Color tells you if it is Gram + or Gram -

Throat swab from red inflamed throat – Streptococcus pyogenes, Streptococcus pneumonia and Streptococcus epidermidis ( Enterococcus facecalis)

Gram stain results

Bacteria name	Gram stain result	Shape of bacteria

The use of blood agar allows the observer to see the presence of enyzmes that bacteria may produce.

Add a picture of the different reactions that are seen on the Blood Agar

Cite your references! Needed to get points

Conclusion

What is the ingredient is selective in the CNABlood agar plates? What macromolecules are damaged by these drugs? Where are these macromolecules in gram-negative bacteria?

What ingredient is the differential ingredient in these plates?

Streptococcus pyogenes makes what protein that can lyse RBC? Describe the reaction that occurs when Streptococcus pyogenes is grown on the CNABlood agar plates?

Streptococcus pneumonia makes what protein that can cause damage to the RBC? Describe the reaction that occurs when Streptococcus pneumonia is grown on the CNABlood agar plates?

Enterococcus facecalis does not makes what protein that can cause damage to the RBC? Describe the reaction that occurs when Enterococcus facecalis is grown on the CNABlood agar plates?