LAB BIOCHEM

1. Why it is necessary to make sure the LB-agar solution is cool enough before adding the ampicillin? What would happened if you add antibiotic when the solution is too hot?

2. Why the word gently emphasized so often in the procedure for making competent cells and what is happening to the cells during transformation?

3. Why do we put the LB-agar plates after transformation upside down in an incubator?