worksheet

Lab – Kirby Bauer Assay

Name____________________________ Date________________________

Section__________________________

Title:

Purpose:

Methods: Lab book page 447 – 451 video observations posted on Brightspace

https://www.youtube.com/watch?v=Np87w5kCL-4

Obtain one colony with swab

Swipe back and forth with swab to inoculate the plate (spread lawn of bacteria)

Place disk on the surface of the plate

Tap disk gently to ensure the disk stays in place

Incubate at 37C for 24 hours

Inspect the plates for Zone Of Inhibition (ZOI) around each antibiotic soaked disk

Measure the ZOI in mm

Use the chart below to identify which antibioitcs the bacteria can be killed with

Results observed:

Use Kirby-BauerData for lab report powerpoint to fill in the table

All measurement are in millimeters

Antibiotic in disk	Resistant	Intermediate	Susceptible
Example-C	<3	4-6	>7
Ciprofloxin -CIP	<15	16-20	>21
Kanamycin - K	<13	14-27	>18
Polymyxin -PB	<8	9-11	>12
Tetracycline -TE	<14	15-18	>19
Streptomycin -S	<11	12-14	>15
Trimethoprim/Sulfamethoxazole- SXT	<10	11-15	>16
Penicillin -P	<28		>29
Amoxicillin/Clavulanic Acid - AMC	<15		>16

Conclusion:

1)Name 4 mistakes that the person wearing white gloves made in setting up the KB assay in the you tube video

2)When this conclusion is finished there will be the site of action of 8 different antimicrobial compounds plus the drug to stop the bacterial virulence factor.

This is a review of the antibiotics and their site of action

For each antibiotic CIP, K, PB, TE, S, SXT, P and isoniazid (not in pictures but in lab 7) tell the site of action. For AMC explain what clavulanic acid does to allow amoxicillin to be more effective.

This means there will be an least nine sentences below

Based on the information in the results section of this lab report which antibiotic should not be used to treat this patient?Expalain

Based on the information in the results section of this lab report which antibiotic would be the best to use to treat this patient? Expalin