assigment
hwolopa
Lab7-MicrobialControlMAC1.docx“Lab 7 Microbial Control” “BIO250L”
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Effect of Chemical Germicides on Bacterial Growth”
“Table 3: Experiment Results”
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“Sample” |
“Germicide” |
“Zone of Inhibition (mm)” |
“Relative Effectiveness” |
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“Skin” |
“10% Bleach” |
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“70% Isopropyl Alcohol” |
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“Hibiclens™” |
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“Control” |
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“Nose” |
“10% Bleach” |
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“70% Isopropyl Alcohol” |
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“Hibiclens™” |
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“Control” |
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“Throat” |
“10% Bleach” |
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“70% Isopropyl Alcohol” |
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“Hibiclens™” |
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“Control” |
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“Shoe” |
“10% Bleach” |
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“70% Isopropyl Alcohol” |
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“Hibiclens™” |
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“Control” |
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“Post-Lab Questions”
“1. Which germicide was the most effective? Was the same germicide most effective for bacteria isolated from the different sources? What could explain this finding?”
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“2. Which germicide was the least effective? Was the same germicide least effective for bacteria isolated from different sources? What could explain this finding?”
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“3. Were any bacteria from a specific source most resistant to the different germicides? If so, which source?”
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“4. Did the dry filter paper serve as a positive or negative control?”
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“5. Look up the chemical formulas for bleach, isopropyl alcohol, and chlorhexidine gluconate (the active ingredient in Hibiclens). How does each chemical achieve its germicidal properties?”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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“Experiment 2- Measuring Antibiotic Resistance”
“Table 4: Experiment Results”
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“Antibiotic” |
“Zone of Inhibition (mm)” |
“Relative Effectiveness” |
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“Ampicillin” |
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“Kanamycin” |
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“Penicillin” |
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“Control” |
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“Post-Lab Questions”
“1. Which antibiotic was most effective in killing the bacterial lawn? Which was the least effective?”
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“2. What is the mechanism of action for each of the antibiotics used in this experiment?”
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“3. If a population of bacteria evolves resistance to penicillin, are these bacteria more likely to be also resistant to ampicillin or kanamycin? Explain your reasoning.”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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