assigment

“Lab 6 Microbial Ecology” “BIO250L”

Student Name: Click here to enter text.

Access Code (located on the lid of your lab kit): Click here to enter text.

“Experiment 1- Mold Growth on Bread and Fruit- What are Optimal Conditions for Fungal Growth?”

“Table 1: Growth Results”

“Condition”	“Extent of Growth”
	“Day 2”	“Day 4”	“Day 6”	“Day 8”	“Day 10”	“Day 12”	“Day 14”
“Bright- Control”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Bright- Water”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Bright- Sugar”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Bright- Lemon”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Bright- Vinegar”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Dark- Control”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Dark- Water”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Dark- Sugar”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Dark- Lemon”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.
“Dark- Vinegar”	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.	Click here to enter text.

“Table Key:” “0 = no growth”

“1 = little/some growth (1-25%)”

“2 = moderate growth (26-50%)”

“3 = heavy growth (51-75%)”

“4 = extensive growth (76-100%)”

“Post-Lab Questions”

“1. Which condition produced the most mold growth? The fastest? The least? The slowest?”

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“2. Based on your results, what conditions are most favorable for mold growth on bread and apples?”

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“3. Would these conditions apply to all fungal growth? Why or why not?”

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“4. How would changing the type of bread (fresh from a bakery, no preservatives vs. prepackaged with preservatives) affect the results? Describe an experiment that would test your hypothesis.”

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“5. How would changing the incubation temperature of the bags affect the results? Describe an experiment that would test your hypothesis.”

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“6. Look up the pH of lemon juice and vinegar. Based on your results and your knowledge of favorable environmental conditions for fungal growth, what can you conclude about the effect of pH on growth? How would making the pH more basic affect growth?”

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“7. What is the source of the mold that grew on the samples?”

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“Insert photo of your cultures after incubation with your name clearly visible in the background:”

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“Experiment 2- Fluid Thioglycollate Medium to Assess the Effect of Oxygen on Bacterial Growth”

“Table 2: Experiment 2 Results”

“Sample Location”	“Growth Location in FTM”	“Oxygen Category”
“Control”	Click here to enter text.	Click here to enter text.
“Skin”	Click here to enter text.	Click here to enter text.
“Nose”	Click here to enter text.	Click here to enter text.
“Throat”	Click here to enter text.	Click here to enter text.
“Shoe”	Click here to enter text.	Click here to enter text.

“Post-Lab Questions”

“1. What does the pink band in the FTM indicate?”

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“2. What would be the effect of using FTM tubes with broad pink zones?”

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“3. Provide an explanation as to why bacteria from each location displayed its pattern of growth?”

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“4. Why is it important to pull the inoculating needle out of the FTM via the same path you inserted it?”

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“5. How would you explain the presence of bacterial growth in the control FTM tube?”

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“Insert photo of your cultures after incubation with your name clearly visible in the background:”

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