Biology (Neuroscience)

BIO310 F

IHC principle lab

Primary antibody against protein raised in RABBIT

Secondary antibody against RABBIT IgG raised in donkey

Blocking contained donkey proteins (to neutralize proteins from Secondary antibody)

Secondary antibodies against RABBIT IgG coupled to BIOTIN

ABC complex – Avin/Biotin/Horseradish peroxidase enzyme

DAB – peroxidase substrate