Nutttt

Triglyceride Determination

Tube Absorbance Reading at 500 nm

Average Absorbance Reading

B 0.000

TG1 0.055

TG2 0.051

S1 0.147

S2 0.116

Calculation of Unknown Sample Concentration

• Use the following formula to determine TG concentration:

• 𝑇𝐺 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = ,-./01. 23 0456/4078.9:;07< 0456/4078. ,-./01. =>07?0/? 0456/4078.

* S (mg/dl)

• Note: " S mg/dl" is the known concentration of the standard (S) as provided by the manufacturer

Total Cholesterol Determination

Tube Absorbance Reading at 520 nm

Average Absorbance Reading

B 0.000

C1 0.067

C2 0.063

S1 0.161

S2 0.150

Calculation of Unknown Sample Concentration

• Use the following formula to determine Total Cholesterol (TC) concentration:

• 𝑇𝐶 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = ,-./01. 2A 0456/4078.9:;07< 0456/4078. ,-./01. =>07?0/? 0456/4078.

* S (mg/dl)

• Note: " S mg/dl" is the known concentration of the standard (S) as provided by the manufacturer