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Care Hope College

Microbiology / Lab Chap 3 and 4

Instructor: Dr. Gesner Cesar

Name: __________________________________________ Date: _______________________________

1-Part A

Definition

Staining, Smear, mordant, Negative staining,

_______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

2- Which of the following places the steps of the Gram stain in the correct order?

1-Alcohol-acetone

2-Crystal violet

3-Safranin

4-Iodine

Ans: ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

3-What are the differences between gram positive and gram-negative bacteria?

4- Which of the following terms best describes the cell in this figure?

A) peritrichous flagella

B) amphitrichous flagella

C) lophotrichous flagella

D) monotrichous flagellum

E) axial filament

5- Please complete the following bacterial figure.

6) Label the following parts of a Microscope.

7) Please label the shape of these bacteria.

A________________________________________B__________________________________

C________________________________________D__________________________________

E________________________________________F__________________________________

G________________________________________H__________________________________

8) Why the acid-fast bacteria stay red and the non-acid-fast turn blue?

Acid –Fast Staining Procedure

Ans:__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

10-How do the Mycobacterium and Nocardia react to acid-fast stain?

11-Mention 3 special staining and Identify the following staining?

$H:\48496 Tortora IRDVD\Working Files 48496\JPEG\ch03_unlabeled\figure_03_14a_unlabeled.jpg$

(a)___________________________________________________________________________

$H:\48496 Tortora IRDVD\Working Files 48496\JPEG\ch03_unlabeled\figure_03_14b_unlabeled.jpg$

(b)__________________________________________________________________________

$H:\48496 Tortora IRDVD\Working Files 48496\JPEG\ch03_unlabeled\figure_03_14c_unlabeled.jpg$

( c )_____________________________________________________________________________

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