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BIO-105-online Lab 2 Name:_________________________

Introduction to Biology-BIO105

Lab 2 Introduction to the Light Microscope

Introduction

A microscope is an instrument that magnifies an object so that it may be seen by the observer. Because cells are usually too small to see with the naked eye, a microscope is an essential tool in the field of biology. In addition to magnification, microscopes also provide resolution, which is the ability to distinguish two nearby objects as separate. A combination of magnification and resolution is necessary to clearly view specimens under the microscope. The light microscope bends a beam of light at the specimen using a series of lenses to provide a clear image of the specimen to the observer.

In this lab, parts of the microscope will be reviewed. Students will learn proper use and care of the microscope and observe samples from pond water

Objectives: The objective of this lab is to examine the function of each part of a microscope and follow step-by-step instructions on how to focus on a specimen. They also review the proper use and care of a microscope and test their knowledge in two drag-and-drop exercises.

Parts of the microscope

(adapted from Wisc-online OER)

Magnification

Your microscope has 4 objective lenses: Scanning (4x), Low (10x), High (40x), and Oil Immersion (100x). Each of the different types of objective lens magnifies an image differently by working with the ocular lens.

In addition to the objective lenses, the ocular lens (eyepiece) has a magnification. The total magnification is determined by multiplying the magnification of the ocular and objective lenses.

Basic Care of the Microscope

• Always carry a microscope with both hands when transporting from and back to the microscope cabinet (or when transporting it to any new location). Remove slide from stage before returning to cabinet.

• Clean eyepieces with lens tissue before and after using.

• Use care with prepared slides (most are expensive). Clean with lens tissue. Transport prepared slides in the provided cardboard slide tray.

• Always start viewing using the scanning power (4X) objective first – focus – then switch to higher powers if needed. Switch back to scanning power objective when removing slide.

Procedure : The Parts of the Microscope

1. Go How to Use a Microscope website (https://www.wisc-online.com/learn/natural-science/life-science/bio905/how-to-use-a-microscope)

2. Click and drag to rotate the microscope

3. Learn about the different parts of the microscope and its functions. Rotate the microscope to view all the different parts.

4. Match the different parts correctly and then take a screenshot (using Snagit or Jing) to record your successful completion of the activity.

5. Submission for grade: Upload under Moodle the screenshot and a short one page write up with the 12 different parts of the microscope and its various functions. Be sure to use the table below in your lab submission.

Suggested Virtual Cleanup Procedure

1. Store microscope with the scanning objective in place and the stage in its lowest position.

2. Wrap cords around microscope.

3. Replace slides to original slide tray.

The Parts of the Microscope

Parts of the Microscope	Description of the function
Nosepiece
Course Knob
Fine Focus
Stage
Stage clips
Ocular Lens
Objective Lens
diaphragm
Microscope Arm
Microscope Base
condenser lens
Eyepiece tube

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