Lab2
Lab 2- The Chemistry of Life
Your Name:
INSTRUCTIONS:
· On your own and without assistance, complete this Lab 2 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus).
· To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions.
· Save your Lab 2 Answer Sheet in the following format: LastName_Lab2 (e.g., Smith_Lab2).
· You should submit your document as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility.
Pre-Lab Questions
What is the primary source of carbohydrates for humans?
Name one type of food that you eat that contain significant amount of carbohydrates.
What are the subunits that nucleic acids are made of? Briefly explain the difference between DNA and RNA.
How is the chemical composition of lipids different than the three other macromolecules? Name one reason why we need fat in our diet.
Briefly describe three different roles of proteins in your body and name one type of food that you eat that has high protein content.
Experiment 1: Testing for Biomolecules
Data Tables
Table 1: Testing for Proteins Results
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Sample |
Initial Color |
Hypothesis (Protein +/-) |
Final Color |
Protein Present? |
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Albumin (1) |
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Gelatin (2) |
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Glucose (3) |
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Water (4) |
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Table 2: Testing for Reducing Sugars Results
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Sample |
Initial Color |
Hypothesis (Reducing Sugar +/-) |
Final Color |
Reducing Sugar Present? |
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Potato (1) |
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Onion (2) |
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Glucose (3) |
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Water (4) |
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Table 3: Testing for Starch Results
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Sample |
Initial Color |
Hypothesis (Starch +/-) |
Final Color |
Starch Present? |
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Potato (1) |
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Onion (2) |
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Starch (3) |
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Water (4) |
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Table 4: Testing an Unknown - Proteins
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Sample |
Initial Color |
Hypothesis (Protein +/-) |
Final Color |
Protein Present? |
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Positive Control: |
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Negative Control: |
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Unknown |
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Table 5: Testing an Unknown – Reducing Sugars
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Sample |
Initial Color |
Hypothesis (Reducing Sugar +/-) |
Final Color |
Reducing Sugar Present? |
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Positive Control: |
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Negative Control: |
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Unknown |
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Table 6: Testing an Unknown – Starch
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Sample |
Initial Color |
Hypothesis (Starch +/-) |
Final Color |
Starch Present? |
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Positive Control: |
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Negative Control: |
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Unknown |
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Post-Lab Questions
1. Insert 1 picture for each of the 6 tests here:
Describe the experimental protocol you developed and followed when testing the unknown. Be sure to include your hypothesis.
Were your hypotheses confirmed by these experiments? If not, what did you find most surprising about the results?
What can you conclude about the unknown solution based on your results?
How did you use parts 1 – 3 of this experiment to inform your experimental design in part 4?
What were your positive and negative controls for each biomolecule when testing the unknown? Explain why you chose each control.
Come up with 2 – 3 practical, real-world scenarios in which testing for biomolecules would be beneficial and describe them.
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