Anatomy and physiology labs ||

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Introduction to ScienceExperiment 1 Kidney Filtration Experiment Inventory

Labware 30 cm Dialysis Tubing

Pipette

(2) 250 mL Beakers

100 mL Graduated Cylinder

10 mL Graduated Cylinder

Materials 2 Small Rubber Bands

3 mL Congo Red Stain

3 mL Yellow Food Coloring

*Water

Note: You must provide the materials listed in *red.

© eScience Labs, 2016

EXPERIMENT 1: KIDNEY FILTRATION

The kidneys filter blood, removing waste. In this experiment, you will simulate kidney filtration. The dialysis bag will represent part of the kidney and the solution containing Congo Red, yellow food coloring, and water will represent blood as it enters the kidney through the renal artery. As the experiment progresses, notice the filtration that occurs within the dialysis tubing, and the substanc es that result from filtration.

PROCEDURE

1. Use the 100 mL graduated cylinder to measure and fill a 250 mL beaker with 200 mL of water. Place the dialysis tubing in

the beaker and submerge it for 10 minutes.

2. Remove the tubing from the water after 10 minutes. Use your index finger and thumb to rub the sides of the dialysis tubing

open. This will create a tube-like shape.

3. Secure a small rubber band around the bottom of the dialysis tubing to seal it. Wrap the rubber band around the dialysis

tubing as many times as possible.

4. To test that the dialysis tubing will not leak, pour a small amount of water into the tubing. If water leaks out of the bottom, the

rubber band is not tight enough. If water does not leak, pour the water out of the tubing into a sink and set the tubing aside.

5. If the volume of water in your 250 mL beaker has decreased, refill it with 200 mL of water.

6. Use the 10 mL graduated cylinder to measure 3 mL of Congo red stain. Then, pour it into the empty 250 mL beaker, and

rinse the graduated cylinder.

7. Use the 10 mL graduated cylinder to measure 3 mL of yellow food coloring. Then, pour it into the same beaker as the Congo

red stain, and rinse the graduated cylinder.

8. Use the 10 mL graduated cylinder to measure 5 mL of water. Then, pour it into the same beaker as the Congo red stain and

yellow food coloring.

9. Use a pipette to mix the solution of Congo red, yellow food coloring, and water.

10. Pipette 10 mL of the dye solution into the dialysis tubing. Then, seal the dialysis tubing by wrapping a second rubber band

around the top of it.

11. Observe the solution in the dialysis tubing and the water in the 250 mL beaker. Record in Table 1 what solutions are present

in each container before the experiment begins.

12. Place the sealed dialysis tubing into the 250 mL beaker containing 200 mL of water.

13. Wait 60 minutes. Look for any diffusion that may have occurred through the dialysis tubing (inbound or outbound). Then,

record which solutions are present in each container in Table 2.

© eScience Labs, 2016

Data SheetExperiment 1 Data Sheet Table 1: Solutions Present in Each Container Before 60 Minutes Submersion

Solution Dialysis Tubing Beaker Congo Red

Yellow Food Coloring

Table 2: Solutions Present in Each Container After 60 Minutes Submersion

Solution Dialysis Tubing Beaker Congo Red

Yellow Food Coloring

© eScience Labs, 2016