Anatomy and physiology labs 2, Lab 10

Experiment 4Blood Typing Experiment Experiment Inventory

Materials Blood Sample A

Blood Sample B

Blood Sample C

Anti-A Serum

Anti-B Serum

Anti-Rh Serum

(9) Toothpicks

*Permanent Marker

Labware 12-Well Plate

Note: You must provide the materials listed in *red.

EXPERIMENT 4: BLOOD TYPING EXPERIMENT Blood typing is an important part of medicine. Antigens, which are specific glycoproteins on the surface of red blood cells, can agglomerate when a foreign antibody is present in the circulatory system. Agglutination can occur when multiple blood types are mixed if RBCs with different antigens are in the same environment. This is because their respective antibodies will react to the foreign antigens. In this experiment, you will view this reaction by mixing simulated blood samples and antibodies. Agglutination will occur if the antibody and corresponding foreign antigen are mixed.

PROCEDURE 1. Use the permanent marker and Figure 8 to label the 12-well plate. The fourth column (on the far right side of the plate) will

not be used in this experiment.

2. Place one drop of blood sample A into wells A1 – A3.

3. Place one drop of blood sample B into wells B1 – B3.

4. Place one drop of blood sample C into wells C1 – C3.

5. Place one drop of anti-A serum into wells A1, B1, and C1.

6. Place one drop of anti-B serum into wells A2, B2, and C2.

7. Place one drop of anti-C serum into wells A3, B3, and C3.

8. Use a clean toothpick to mix the blood sample and typing sera in each well. Be sure to use a new toothpick for each well.

9. Wait 2 minutes. Then, observe each well for agglutination, which indicates a positive result. Note that agglutination will be observable as a precipitate when working with simulated blood samples.

10. Record your observations in Table 4. Determine the blood type of each sample based on the results.

Figure 8: Labeling diagram for the 12-well plate.

© eScience Labs, 2016

Data Sheet Experiment 4 Data Sheet Table 4: Blood Typing Results

A (Blood Sample A)

B (Blood Sample B)

C (Blood Sample C)

1 (Anti-A Serum)

2 (Anti-B Serum)

3 (Anti-Rh Serum)

Blood Type

© eScience Labs, 2016