biology

BIOLOGY 211 LA-4 Enzymes Page 2 of 2

Effect of temperature on the activity of turnip peroxidase

(If you are struggling with any of the questions, I recommend thoroughly reading through the first two pages of Enzyme Lab introduction.)

Question:

Null hypothesis (avoid using the word “will” in your hypothesis):

(If you need help with hypothesis or independent and dependent variables, I suggest reviewing background information from Diffusion Lab.)

In this experiment, what are the independent and dependent variables?

independent:

dependent:

What is the name of the enzyme in this enzyme-mediated reaction?

What is the substrate molecule in this enzyme-mediated reaction? _______________________

What is/are the product(s) produced in this enzyme-mediated reaction?

Which tube served as a negative control? __________________________

How do you know it is a negative control?

Paste in your graph below:

Do the data support the null hypothesis? Explain, using the data (numbers) you obtained to support your conclusion.

Of those tested, what is the optimum temperature for turnip peroxidase? _______________

Why is activity lower at other temperatures?

Effect of pH on the activity of turnip peroxidase

Question:

Null hypothesis (avoid using the word “will” in your hypothesis):

In this experiment, what are the independent and dependent variables?

independent:

dependent:

Which tube served as a negative control? __________________________

Which tube served as a positive control? (which would you to predict to have optimal pH for turnip peroxidase)

__________________________

Paste in your graph below:

Do the data support the null hypothesis? Explain, using the data (numbers) you obtained to substantiate your conclusion.

Of those tested, what is the optimum pH for turnip peroxidase? _______________

Why is activity lower at other pH values?

EXPERIMENT PROPOSAL