"Identification of unknown microbe"

InstructionfortheBIOL3321LFinalLabreport20FA.docx

Home >Biology homework help >"Identification of unknown microbe"

Instruction for the BIOL 3321L Final Lab report (50 points)

Since we could not continue to characterize bacteria that were isolated from human or class room in classes, students must submit the final lab report with the given information. Please read this instruction carefully and submit your report by 11/20/2020 5 PM to the BIOL3321L dropbox in D2L. According to late submission policy in the syllabus, no point will be given for late submission. If you have any questions, contact the instructor ([email protected]) ASAP since to write the final report is time-consuming.

1. Instructor will assign one unknown bacterium to each group in BIOL 3321L class (including both hybrid and online students). Students can submit the report as a group or individuals. If you choose group submission, you need to clearly show who contributes to write the report. (e.g. Group A has 3 members, but two students form 1 group and one student work individually. In such case, the students who made a team must show name of both students on the provided specific format). To share the information over groups are penalized (e.g. Group A and Group B cannot use the same descriptions, tables, figures).

2. Read lab manuals 2 & 3 to find how to write a final lab report. Your lab report must have the following items,

1) Title, Name, Student ID number and Affiliation (Cover page with specific format will be provided)

2) Summary (less than 250 words)

3) Introduction

4) Materials & Methods - Show all materials and methods used to obtain the data. Students can describe everything or use a table to describe materials and methods concisely.

5) Results - Show all results with one table and describe the data.

6) Discussion & Conclusion - The section must include the 4-page diagnostic key which supports your conclusion

7) References

*You can find the rubric in the Lab manual 3. The instructor will grade the final lab report with this rubric.

*Students need to make figure(s) to show the diagnostic keys (both Gram-positive and negative keys must be submitted). Alternatively, the completed key can be scanned and submitted to the dropbox with either JPEG or PDF format. If you cannot access a scanner and need to take photos by your cell phone/camera, low resolution of the pictures/files must be avoided. The instructor will not provide any points if characters are not clear for any reasons.

Cover page (Use this specific format)

RESEARCH REPORT

IDENTIFICATION OF UNKNOWN MICROBES

Course: Savannah State University - 3321L / 2020 Spring

Section: 1 (1 PM) or Section 2 (4 PM) (highlight one)

Group Name (type your group Number):

Submission option: Individual or Group (*up to 4 members per repot) (highlight one)

Authors (Student name and 915) who contribute to write this report

Experiments & Data

Please check the assignment table and find experimental data corresponding to your group. No point will be given to the reports that chose the wrong data set. Contact the instructor ([email protected]) if you have any questions.

Group and Assignments

Group Name for Lab report	Student Name	Assigned Data Set (See below)
1A	Bennett, Kori J. Bradley, Kia K. Brewton, Deveon A. Broadwater, Dylan	Data A
1B	Brown, Kennedy D. Coleman, Robyn F. Exum, Audra L. Huynh, Huong	Data B
1C	Jackson, Jamya Jackson, Morgan D. Johnson, Mary E. Littlejohn, Jazmine A.	Data C
1D	Pellington, Toni A. Peron, Hope C. Roby, Dechelle Rosier, Aisa M.	Data D
1E	Smith, Jharnika A. Soyemi, Oluseyi B. Taylor, Branden R.	Data E
1F	Tinch, Regia L. Turner, Lakaya R. Willis, Alexandria R.	Data F
2A	Butler, Dajour T. Byrne, Briana E. Carter, Jataya C. Davis, De`jah L.	Data A
2B	Edwards, Amelda L. Frazier, Lester D. Gainor, Jantisha T. Garrett, Daja M.	Data B
2C	James, Amiya Jones, Fatima T. Lukusa-Sawelena, Bitota K. Marable, Alicia J.	Data C
2D	Mock, Dayzhia T. Odom, Lashonna T. Ohanu, Joshua C. Rasheed, Tupac A.	Data D
2E	Rivera Pagan, Charline Simmons, Sydney M. Smith, Keshawna A. Spencer, Deontis R.	Data E
2F	Tucker, Amia L. Ware, Timesha M. Weaver, Amanda D.	Data F
2G	Weston, Marnisha E. Williams, Jasmine N. Young, Tytiana	Data A

Read the information below and write a report. Students need to research materials and methods corresponding to the experiments/tests. The majority of the information for the tests is available in Lab activity notes and Lab manuals, but students need to research some tests by themselves. The table below showed observations and the report must show results that are interpreted by the students.

Data A

Isolation	Your bacterial was isolated from soil with T-soy plate
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was purple after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria Developed a deep rose color in the culture 15 minutes following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow
Lactose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Spore formation	Spores/endospores were observed after staining with malachite green
Pigment	Bacteria did not show pigments

Data B

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow
Lactose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria did not show pigments

Data C

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	bacteria did not show diffusion in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Lactose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria did not show pigments

Data D

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical turned to pink.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Lactose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria show pigments (blue, yellow, red, etc)

Data E

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Addition of Kovac’s reagent to bacteria in the test tube formed cherry red reagent layer.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Lactose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria did not show pigments.

Data F

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was spherical.
Gram’s staining	The color of the bacteria was purple after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria grew on the plates and made the media yellow.
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Lactose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria produced yellow pigments.