A&P 1 lab
Freda@2017
IHCChistologyslidesusetostudyandcreatetissuedrawings.pdf
Histology Photos
• These are photos of our (IHCC) actual slide
collection.
• What you would view if we had in-person labs.
• Use these to study for exam and to create your
Tissue Drawings.
• Test images will be from our collection only.
• Tissue Drawings must be from our collection only.
1
Histology Photos
• Remember: Tissues do not exist in isolation!
Organs are made of multiple tissues.
• Slides are taken from real structures/organs,
therefore a microscope slide rarely shows one tissue
type!
• Most of these slides will show multiple tissue types.
2
If I told you I saw deer in this field, how would you
look for them?
3
Would you zoom in and start like this?
Nope, tree trunk.
Nope, grass
etc
This would take forever, and you might miss something.
4
Better to start with the big picture, scan the meadow,
find potential deer, then zoom in
5
6
Field of View
Large Field of View
Scan
Zoom in on detail
Smaller Field of View
Histology in Practice
When studying histology (or anything with a
microscope), one of the most important skills to learn
is how to find something on a slide.
8
9
Name Magnification
Total
Magnification
(w/ Ocular
Lens)
Scanning
Objective 4x 40x
Low Power
Objective 10x 100x
High Power
Objective 40x 400x
Oil Immersion 100x 1000x
Histology in Practice
• Follow the same approach as you did with the deer:
SCAN FIRST!
• Use the scanning objective lens (4x) to get a “big
picture” of the organ/structure on the slide
• You may see multiple tissues!
• Use your knowledge of tissue locations to determine
which may be present
• e.g. Is this part of the lung? What type of epithelium could you
see?
10
Histology in Practice
• When you find an area of interest, then you will
zoom in with 10x, then 40x objectives
• We will not use 100x (oil immersion) objective in this
lab.
• Always remember to mark total magnification in
your notes and assignments.
“40x” implies total mag = 40x
“4x objective” implies total mag = 40x
11
Histology in Practice
• These photos will be of organ slides.
• Each may have multiple tissues
• This presentation will follow practical sequence:
• Scan, low power, high power.
• All labels indicate total magnification
• Various tissues are best viewed at different magnifications
• Photos on lab exam will be typical, showing tissue at
magnifications at which they are recognizable
12
Lab exam question example:
1. Name this tissue (entire slide)
– include major category and subtype.
2. Name this special feature (inside circle) 13
Mesothelium
40x
100x
400x
400x
Kidney Cortex with glomeruli
40x
100x
400x
400x
Kidney Medulla with tubules
40x
100x
400x
400x
Small Intestine
40x
100x
400x
400x
Trachea
40x
100x
400x
100x
400x
100x
40x
100x
400x
Thick Skin from Palmar Surface
40x
100x
400x
100x
400x
Skin
40x
Mucous Membrane Digestive Tract
40x
100x
400x
Urinary Bladder
40x
100x
100x
400x
Abdominal Fat
40x
100x
400x
400x
Areolar Tissue
40x
100x
400x
400x
Tendon
40x
100x
100x
Aorta (elastic artery)
40x
100x
400x
400x
Lymph Node
40x
100x
400x
400x
Blood
40x
400x
400x
400x
400x
External Ear
40x
100x
400x
400x
Intervertebral Disc
40x
100x
400x
400x
Ground Bone
40x
100x
400x
400x
Skeletal Muscle
40x
400x
Arteries Cross Section
40x
100x
400x
Heart
40x
100x
400x
400x
Spinal Cord Smear
40x
100x
400x
400x
