MICROBIOLOGY LAB
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IDENTIFICATIONANDANALYSISOFKLEBSIELLAPNEUMONIAEMODUPEreview.docxModupe Epebinu 2
Modupe Epebinu 2
Identification And Analysis Of Klebsiella pneumoniae.
Modupe Epebinu
Baltimore City Community College
Professor Mark Dreyfuss
April 29, 2021
Introduction
Background information
Dealing with an unknown sample in the laboratory requires a number of tests for potential elimination processes. When given an unknown sample, one of the critical steps to take is ensuring that you have as many portions of the sample as possible. These enable one to carry out several tests where each test may infer the possibility of a number of pathogens or items under study. Therefore, examining the sample's nature helps to decide on the rightful tests to be carried out, whether in a solution or solid form. The number of tests determines the number of potions to be made; they should be enough for exhaustive confirmations and eliminations. The sample's nature plays a big role in whether you need to have test solutions or just a physical examination. Comment by Moshe Dreyfuss: What does this mean?
Purpose objective
Carrying out a test may give results that are not very specific. This is because some samples have similar characteristics. It may, therefore, not be ideal for making conclusions on findings that are depicting multiple inferences hence the need to have more tests to come up with a specific finding of the given sample. Similarities in samples' reactions can be very confusing if only a few tests are done for verification and elimination. Comment by Moshe Dreyfuss: The idea is to eliminate negative results and concentrate on positives that lead to the correct answer. Also, why are you trying to identify the organism?
Materials and methods
The materials required included four agar plates, blood agar, MacConkey agar, Hektoen enteric agar, XLD agar, and a lab worksheet. Also, other test sets were required to come to a conclusive argument about the sample's specificity. The tests included Glucose, Lactose, Sucrose, citrate, Lysine decarboxylase, Malonate, ONPG, and Methyl red. There were two test sets; A and B. We carried out these tests independently, and their results were recorded and analyzed separately. Test set A involved the preparation of the 4 different agar mediums. The second test set that is test set B involved the use of 8 test tube mediums to test the specificity of the sample. Comment by Moshe Dreyfuss: You need to start at the beginning. What about gram stain? What did the results tell you to use?
Test set A comprised of 4 agar tests; the first test was viewing the samples for new characteristics. We took one agar plate and prepared the blood agar. Added one portion of the sample into the blood agar. We estimated the observed specimen's diameter; we also observed the color change of the agar medium and other physical properties of the medium. We recorded the results in the lab worksheets. The second test was a repeat of the first test. However, instead of using the blood agar, we tested using MacConkey agar. The third test was a repeat of the first test. However, instead of using the blood agar, we repeated it using the Hektoen enteric agar. The fourth test was a repeat of the first test. However, instead of using blood agar, we replaced it with the XLD agar. All the above tests were used to identify the physical characteristics of the unknown sample in terms of sizes of the elements contained in the sample, the shape, and the color and body segments of any identified bodies.
For Test set B; we used the test-tube mediums; Glucose, Lactose, Sucrose, citrate, Lysine decarboxylase, Malonate, ONPG Methyl red to carry out the tests. We labeled the test tubes based on the mediums they contained. In each test tube of a given medium, we dropped a portion of the unknown sample. We left the setup undisturbed for 20 minutes. We observed each test tube and recorded the results in the lab worksheet. Comment by Moshe Dreyfuss: Use the API 20e
Results
In test set A, in the blood agar, the colonial morphology was mucoid with a diameter of 4 millimeters. On the MacConkey agar plate, large, mucoid as well as pick colonies formed. They were 4 millimeters in diameter. On the Hektoen enteric agar, there was the formation of yellow colonies. In the XLD agar, the colonies formed were yellow.
The following table shows the results for the test set B
Solution |
Result |
Glucose |
Positive |
Lactose |
Positive |
Sucrose |
Positive |
Citrate |
Positive |
Lysine decarboxylase |
Positive |
Malonate |
Positive |
ONPG |
Positive |
Methyl red |
Positive |
