Reflection Paper

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How to Read a Scientific Article

Mary Purugganan, Ph.D. [email protected]

Jan Hewitt, Ph.D. [email protected]

Cain Project in Engineering and Professional Communication

Reading a scientific article is a complex task. The worst way to approach this task is to

treat it like the reading of a textbook—reading from title to literature cited, digesting

every word along the way without any reflection or criticism. Rather, you should begin

by skimming the article to identify its structure and features. As you read, look for the

author’s main points. Generate questions before, during, and after reading. Draw

inferences based on your own experiences and knowledge. And to really improve

understanding and recall, take notes as you read. This handout discusses each of these

strategies in more detail.

1. Skim the article and identify its structure.

Most journals use a conventional IMRD structure: An abstract followed by Introduction,

Methods, Results, and Discussion. Each of these sections normally contains easily

recognized conventional features, and if you read with an anticipation of these features,

you will read an article more quickly and comprehend more.

Features of Abstracts

Abstracts usually contain four kinds of information:

• purpose or rationale of study (why they did it)

• methodology (how they did it)

• results (what they found)

• conclusion (what it means)

Most scientists read the abstract first. Others—especially experts in the field—skip right

from the title to the visuals because the visuals, in many cases, tell the reader what kinds

of experiments were done and what results were obtained. You should probably begin

reading a paper by reading the abstract carefully and noting the four kinds of information

outlined above. Then move first to the visuals and then to the rest of the paper.

Features of Introductions

Introductions serve two purposes: creating readers’ interest in the subject and providing

them with enough information to understand the article. Generally, introductions

accomplish this by leading readers from broad information (what is known about the

topic) to more specific information (what is not known) to a focal point (what question

the authors asked and answered). Thus, authors describe previous work that led to

current understanding of the topic (the broad) and then situate their work (the specific)

within the field.

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Features of Methods

The Methods section tells the reader what experiments were done to answer the question

stated in the Introduction. Methods are often difficult to read, especially for graduate

students, because of technical language and a level of detail sufficient for another trained

scientist to repeat the experiments. However, you can more fully understand the design

of the experiments and evaluate their validity by reading the Methods section carefully.

Features of Results and Discussion

The Results section contains results—statements of what was found, and reference to the

data shown in visuals (figures and tables). Normally, authors do not include information

that would need to be referenced, such as comparison to others’ results. Instead, that

material is placed in the Discussion—placing the work in context of the broader field.

The Discussion also functions to provide a clear answer to the question posed in the

Introduction and to explain how the results support that conclusion.

Atypical Structure

Some articles you read will deviate from the conventional content of IMRD sections. For

instance, Letters to Nature appear to begin with an abstract, followed by the body of the

article. Upon reading, however, you will see that the “abstract” is a summary of the work

filled with extensive introduction (for the purpose of catching the attention of a wide

audience), and the next paragraph begins a description of the experiments.

Therefore, when you begin to read an article for the first time, skim the article to analyze

the document as a whole. Are the sections labeled with headings that identify the

structure? If not, note what the structure is. Decide which sections contain the material

most essential to your understanding of the article. Then decide the order in which you

will read the sections.

2. Distinguish main points.

Because articles contain so much information, it may be difficult to distinguish the main

points of an article from the subordinate points. Fortunately, there are many indicators of

the author’s main points:

Document level

• Title

• Abstract

• Keywords

• visuals (especially figure and

table titles)

• first sentence or the last 1-2

sentences of the Introduction

Paragraph level: words or phrases to look for

• surprising

• unexpected

• in contrast with previous work

• has seldom been addressed

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• we hypothesize that

• we propose

• we introduce

• we develop

• the data suggest

3. Generate questions and be aware of your understanding

Reading is an active task. Before and during your reading, ask yourself these questions:

• Who are these authors? What journal is this? Might I question the credibility of

the work?

• Have I taken the time to understand all the terminology?

• Have I gone back to read an article or review that would help me understand this

work better?

• Am I spending too much time reading the less important parts of this article?

• Is there someone I can talk to about confusing parts of this article?

After reading, ask yourself these questions:

• What specific problem does this research address? Why is it important?

• Is the method used a good one? The best one?

• What are the specific findings? Am I able to summarize them in one or two

sentences?

• Are the findings supported by persuasive evidence?

• Is there an alternative interpretation of the data that the author did not address?

• How are the findings unique/new/unusual or supportive of other work in the field?

• How do these results relate to the work I’m interested in? To other work I’ve read

about?

• What are some of the specific applications of the ideas presented here? What are

some further experiments that would answer remaining questions?

4. Draw inferences.

Not everything that you learn from an article is stated explicitly. As you read, rely on

your prior knowledge and world experience, as well as the background provided in the

article, to draw inferences from the material. Research has shown that readers who

actively draw inferences are better able to understand and recall information.

As an example, in the box below is an excerpt from the Introduction of an article in the

journal Biochemistry (Ballestar et al., 2000). The comments in italics are questions and

inferences that might be drawn by a student reader.

Rett Syndrome is a childhood neurodevelopmental disorder and one of the most common

causes of mental retardation in females Comment: Hmmm…must be related to a gene on

the X-chromosome, with an incidence of 1 in 10000-15000. Comment: How common is

that? Not too likely to happen to me, but there must be several such children born in

Houston every year. Rett syndrome patients are characterized by a period of normal

growth and development (6-18 months) followed by regression with loss of speech and

purposeful hand use. Comment: What happens? Something must be triggered or

activated at late infancy. Patients also develop seizures, autism, and ataxia. After initial

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regression, the condition stabilizes and patients survive into adulthood. Studies of

familial cases provided evidence that Rett is caused by X-linked dominant mutations in a

gene subject to X-chromosome inactivation. Recently, a number of mutations in the gene

encoding the methyl-CpG binding transcriptional repressor MeCP2 have been associated

with Rett Syndrome. Comment: MeCP2 mutations probably cause Rett Syndrome. This

must be an important master-regulator to affect so many processes in the brain. I

wonder what they know about it…

5. Take notes as you read.

Effective readers take notes—it improves recall and comprehension. You may think

you’ll remember everything you read in researching class assignments, professional

papers, proposals, or your thesis, but details will slip away. Develop a template for

recording notes on articles you read, or adapt the template below for use. As you

accumulate a large collection of articles, this template will help you distinguish articles

and quickly locate the correct reference for your own writing. The time spent filling out

the form will save you hours of rereading when you write a Background, Related Work,

or a Literature Review section.

Template for Taking Notes on Research Articles:

Easy access for later use

Whenever you read an article, pertinent book chapter, or research on the web, use the

following format (or something similar) to make an electronic record of your notes for

later easy access. Put quotation marks around any exact wording you write down so that

you can avoid accidental plagiarism when you later cite the article.

Complete citation. Author(s), Date of publication, Title (book or article), Journal,

Volume #, Issue #, pages:

If web access: url; date accessed

Key Words:

General subject:

Specific subject:

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Hypothesis:

Methodology:

Result(s):

Summary of key points:

Context (how this article relates to other work in the field; how it ties in with key issues

and findings by others, including yourself):

Significance (to the field; in relation to your own work):

Important Figures and/or Tables (brief description; page number):

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Cited References to follow up on (cite those obviously related to your topic AND any

papers frequently cited by others because those works may well prove to be essential as

you develop your own work):

Other Comments:

References

Ballestar, E., Yusufzai, T.M., and Wolffe, A.P. (2000) Effects of Rett Syndrome

Mutations of the Methyl-CpG Binding Domain of the Transcriptional Repressor MeCP2

on Selectivity for Association with Methylated DNA. Biochemistry 31, 7100-7106.

Burnett, R. (2001) Technical Communication. 5 th ed. San Antonio: Harcourt College

Publishers.

Zeiger, M. (2000) Essentials of Writing Biomedical Research Papers. 2 nd

Ed. St. Louis:

McGraw-Hill.

Supported by the Cain Project for Engineering and Professional Communication

Rice University, 2004