Ex utero mouse embryogenesis from pre-gastrulation to late organogenesis

Please review th

is prima

ry

article and answer the following questions.

1.

What are some of the conditions that had to be maintained for the growth of

ex utero

embryos.

2.

What day were these embryos grown to

(mice are on embryonic day 20 (E20))

, were they

normal to this point

/ ho

w many development marks were analyzed to determine this

?

3.

What is a factor that would

have to be overcome to continue ex utero development?

4.

Summarize what the authors are showing in Figure 4 of the paper.

5.

The last sentence of the article notes that th

is work sets the stage for expanding ex utero

embryo research

to

different mammalian species and from stem cell aggregated synthetic

embryos.

S

hould we study human embryos with this technique if

they can be

developed

in

culture from stem cells. Why or w

hy not?

Please review this primary article and answer the following questions.

1. What are some of the conditions that had to be maintained for the growth of ex utero embryos.

2. What day were these embryos grown to (mice are on embryonic day 20 (E20)), were they

normal to this point / how many development marks were analyzed to determine this?

3. What is a factor that would have to be overcome to continue ex utero development?

4. Summarize what the authors are showing in Figure 4 of the paper.

5. The last sentence of the article notes that this work sets the stage for expanding ex utero

embryo research to different mammalian species and from stem cell aggregated synthetic

embryos. Should we study human embryos with this technique if they can be developed in

culture from stem cells. Why or why not?