Enzymes lab report

time (sec) tubes 2 and 3 4 ◦ c tubes 4 and 5 24 ◦ c tubes 6 and 7 32 ◦ c tubes 8 and 9 48 ◦ c 20 0.27 0.35 0.49 0.72 40 0.39 0.52 0.7 0.95 60 0.52 0.63 0.84 1.1 80 0.63 0.72 0.94 1.2

100 0.73 0.79 1.02 1.25 120 0.82 0.85 1.08 1.3

Temperature Effect on Peroxidase

A B SO

R B A N

C E

0

0.35

0.7

1.05

1.4

TIME (SEC)

20 40 60 80 100

y = 0.1114x + 0.6967

y = 0.1146x + 0.444

y = 0.0971x + 0.3033

y = 0.1109x + 0.172

tubes 2 and 3 4 ◦ c y = 0.1109x + 0.172 tubes 4 and 5 24 ◦ c tubes 6 and 7 32 ◦ c y = 0.1146x + 0.444 tubes 8 and 9 48 ◦ c

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Temperature Effect on Peroxidase

TIME (SEC)

100 120

tubes 2 and 3 4 ◦ c y = 0.1109x + 0.172 tubes 4 and 5 24 ◦ c y = 0.0971x + 0.3033 tubes 6 and 7 32 ◦ c y = 0.1146x + 0.444 tubes 8 and 9 48 ◦ c y = 0.1114x + 0.6967

�2

�3

4 ◦ c 24 ◦ c RATE ( CHANGE OF ABSORBANCE/SECOND) 0.1109 0.0971

Slope of the Temperature Effect on Peroxidase

R AT

E (

C H

A N

G E

O F

A B SE

R B A N

C E/

SE

C O

N D

)

0.08

0.09

0.1

0.11

0.12

Temperature(◦ C)

4 ◦ c

�1

32 ◦ c 48 ◦ c 0.1146 0.1114

Slope of the Temperature Effect on Peroxidase

Temperature(◦ C)

24 ◦ c 32 ◦ c 48 ◦ c

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