BIOL 362 Unknown Project

Getting Ready for your Laboratory Unknown

Assignment

Colony morphology

• 1) Look at one individual colony that is separated from the others.

• 2) Write down each category and then how each colony looks.

• For example: the colony morphology of the organism highlighted by the arrow is:

Form – entire, Elevation – and so on through the categories. For the unknown, you are only required to complete, Form, Elevation, Margin, Surface, Opacity and Chromogenesis

Gram stain morphology

• Gram stain morphology is composed of 3 parts:

1) Is the stained organism gram- positive or gram-negative?

2) What is the morphology (shape) of the individual organisms?

You can have:

Gram (+) rods(bacilli)

Gram (-) rods (bacilli)

Gram (+) cocci

Gram (-) cocci

3) Are the cells in a specific arrangement?

Test Results

• Everyone will be given their own powerpoint that corresponds to the organism that they are assigned. The unknown worksheet is a PDF on D2L. All unknown results MUST be filled out by hand with a pen, as they would be in the laboratory. • 1) As you look at the PowerPoint slide

for each test, there will be a picture of what happened with your organism. On the worksheet, fill out what the results of the test are. For example: fill out whether this organism on the right is amylase positive or negative, not visual observations about the organism or media.

Summary of Test Results

• In the last section, you will analyze your results and write a summary. Tell me in your own words, how you used your results to come to a presumptive identification.

• Also, draw a flow-chart/ dichotomous key of how you arrived at your results. I have provided a link for use as a resource”

• https://creately.com/blog/diagrams/what-is-a-dichotomous-key/

Identification

• In the spaces provided, write down the identity of your bacterial unknown.

• You are being graded on identifying the proper genus. You will not be penalized for an incorrect species unless you misidentify a species that we covered in the laboratory.

• Know that you are awarded points for each step of the assignment that was done correctly. The final identification is only worth a few extra points.