Exam 3 DNA problems: Work on these problems BEFORE the exam. DO NOT discuss these problems with anyone, as you are expected to work on them on your own. The actual exam questions will require you to have some of these tanswers on hand and document your work. 1. Predict a possible phenotype from the following mutations in the Lac Operon: a deletion in “GGAATTGTGAGCGGATAACAATTTCAC.” 2. Using the BLASTP search, find out which is the closest biological source for this protein? (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins). How is this protein related to one you have worked on before? How closely related are the new protein and the ‘old’ protein in percentage of identity?

>protein Y

MIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQ

GRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRI

GAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLL

RAFYCILEPRSGNHCPAGNSHTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFM

YTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSI

RSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGV

YSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSV

NDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLI

LATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDY

YRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQL

GNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVS

VIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNS

SLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKL

SIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANL

RQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTI

ADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGW

TAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFR

KVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNA

FVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVG

YYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSL

NTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLL

DLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCC

TGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH

3. We will re-analyze our old sequence, the gene that can cause hemophilia in humans,

coagulating factor VIII. The mRNA sequence (as DNA) is shown below. What is the longest

open reading frame (ORF)?

4. What is the longest fragment from this gene you can clone using HindIII (A’AGCTT)?

https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins

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5. If you successfully cloned this longest fragment into pUC19, what are the two possible

restriction patterns on a gel you can expect using XbaI (T’CTAGA)?

>NM_000132.4|coagulating factor VIII

GCTTAGTGCTGAGCACATCCAGTGGGTAAAGTTCCTTAAAATGCTCTGCAAAGAAATTGGGACTTTTCAT

TAAATCAGAAATTTTACTTTTTTCCCCTCCTGGGAGCTAAAGATATTTTAGAGAAGAATTAACCTTTTGC

TTCTCCAGTTGAACATTTGTAGCAATAAGTCATGCAAATAGAGCTCTCCACCTGCTTCTTTCTGTGCCTT

TTGCGATTCTGCTTTAGTGCCACCAGAAGATACTACCTGGGTGCAGTGGAACTGTCATGGGACTATATGC

AAAGTGATCTCGGTGAGCTGCCTGTGGACGCAAGATTTCCTCCTAGAGTGCCAAAATCTTTTCCATTCAA

CACCTCAGTCGTGTACAAAAAGACTCTGTTTGTAGAATTCACGGATCACCTTTTCAACATCGCTAAGCCA

AGGCCACCCTGGATGGGTCTGCTAGGTCCTACCATCCAGGCTGAGGTTTATGATACAGTGGTCATTACAC

TTAAGAACATGGCTTCCCATCCTGTCAGTCTTCATGCTGTTGGTGTATCCTACTGGAAAGCTTCTGAGGG

AGCTGAATATGATGATCAGACCAGTCAAAGGGAGAAAGAAGATGATAAAGTCTTCCCTGGTGGAAGCCAT

ACATATGTCTGGCAGGTCCTGAAAGAGAATGGTCCAATGGCCTCTGACCCACTGTGCCTTACCTACTCAT

ATCTTTCTCATGTGGACCTGGTAAAAGACTTGAATTCAGGCCTCATTGGAGCCCTACTAGTATGTAGAGA

AGGGAGTCTGGCCAAGGAAAAGACACAGACCTTGCACAAATTTATACTACTTTTTGCTGTATTTGATGAA

GGGAAAAGTTGGCACTCAGAAACAAAGAACTCCTTGATGCAGGATAGGGATGCTGCATCTGCTCGGGCCT

GGCCTAAAATGCACACAGTCAATGGTTATGTAAACAGGTCTCTGCCAGGTCTGATTGGATGCCACAGGAA

ATCAGTCTATTGGCATGTGATTGGAATGGGCACCACTCCTGAAGTGCACTCAATATTCCTCGAAGGTCAC

ACATTTCTTGTGAGGAACCATCGCCAGGCGTCCTTGGAAATCTCGCCAATAACTTTCCTTACTGCTCAAA

CACTCTTGATGGACCTTGGACAGTTTCTACTGTTTTGTCATATCTCTTCCCACCAACATGATGGCATGGA

AGCTTATGTCAAAGTAGACAGCTGTCCAGAGGAACCCCAACTACGAATGAAAAATAATGAAGAAGCGGAA

GACTATGATGATGATCTTACTGATTCTGAAATGGATGTGGTCAGGTTTGATGATGACAACTCTCCTTCCT

TTATCCAAATTCGCTCAGTTGCCAAGAAGCATCCTAAAACTTGGGTACATTACATTGCTGCTGAAGAGGA

GGACTGGGACTATGCTCCCTTAGTCCTCGCCCCCGATGACAGAAGTTATAAAAGTCAATATTTGAACAAT

GGCCCTCAGCGGATTGGTAGGAAGTACAAAAAAGTCCGATTTATGGCATACACAGATGAAACCTTTAAGA

CTCGTGAAGCTATTCAGCATGAATCAGGAATCTTGGGACCTTTACTTTATGGGGAAGTTGGAGACACACT

GTTGATTATATTTAAGAATCAAGCAAGCAGACCATATAACATCTACCCTCACGGAATCACTGATGTCCGT

CCTTTGTATTCAAGGAGATTACCAAAAGGTGTAAAACATTTGAAGGATTTTCCAATTCTGCCAGGAGAAA

TATTCAAATATAAATGGACAGTGACTGTAGAAGATGGGCCAACTAAATCAGATCCTCGGTGCCTGACCCG

CTATTACTCTAGTTTCGTTAATATGGAGAGAGATCTAGCTTCAGGACTCATTGGCCCTCTCCTCATCTGC

TACAAAGAATCTGTAGATCAAAGAGGAAACCAGATAATGTCAGACAAGAGGAATGTCATCCTGTTTTCTG

TATTTGATGAGAACCGAAGCTGGTACCTCACAGAGAATATACAACGCTTTCTCCCCAATCCAGCTGGAGT

GCAGCTTGAGGATCCAGAGTTCCAAGCCTCCAACATCATGCACAGCATCAATGGCTATGTTTTTGATAGT

TTGCAGTTGTCAGTTTGTTTGCATGAGGTGGCATACTGGTACATTCTAAGCATTGGAGCACAGACTGACT

TCCTTTCTGTCTTCTTCTCTGGATATACCTTCAAACACAAAATGGTCTATGAAGACACACTCACCCTATT

CCCATTCTCAGGAGAAACTGTCTTCATGTCGATGGAAAACCCAGGTCTATGGATTCTGGGGTGCCACAAC

TCAGACTTTCGGAACAGAGGCATGACCGCCTTACTGAAGGTTTCTAGTTGTGACAAGAACACTGGTGATT

ATTACGAGGACAGTTATGAAGATATTTCAGCATACTTGCTGAGTAAAAACAATGCCATTGAACCAAGAAG

CTTCTCCCAGAATTCAAGACACCCTAGCACTAGGCAAAAGCAATTTAATGCCACCACAATTCCAGAAAAT

GACATAGAGAAGACTGACCCTTGGTTTGCACACAGAACACCTATGCCTAAAATACAAAATGTCTCCTCTA

GTGATTTGTTGATGCTCTTGCGACAGAGTCCTACTCCACATGGGCTATCCTTATCTGATCTCCAAGAAGC

CAAATATGAGACTTTTTCTGATGATCCATCACCTGGAGCAATAGACAGTAATAACAGCCTGTCTGAAATG

ACACACTTCAGGCCACAGCTCCATCACAGTGGGGACATGGTATTTACCCCTGAGTCAGGCCTCCAATTAA

GATTAAATGAGAAACTGGGGACAACTGCAGCAACAGAGTTGAAGAAACTTGATTTCAAAGTTTCTAGTAC

ATCAAATAATCTGATTTCAACAATTCCATCAGACAATTTGGCAGCAGGTACTGATAATACAAGTTCCTTA

GGACCCCCAAGTATGCCAGTTCATTATGATAGTCAATTAGATACCACTCTATTTGGCAAAAAGTCATCTC

CCCTTACTGAGTCTGGTGGACCTCTGAGCTTGAGTGAAGAAAATAATGATTCAAAGTTGTTAGAATCAGG

TTTAATGAATAGCCAAGAAAGTTCATGGGGAAAAAATGTATCGTCAACAGAGAGTGGTAGGTTATTTAAA

GGGAAAAGAGCTCATGGACCTGCTTTGTTGACTAAAGATAATGCCTTATTCAAAGTTAGCATCTCTTTGT

TAAAGACAAACAAAACTTCCAATAATTCAGCAACTAATAGAAAGACTCACATTGATGGCCCATCATTATT

AATTGAGAATAGTCCATCAGTCTGGCAAAATATATTAGAAAGTGACACTGAGTTTAAAAAAGTGACACCT

TTGATTCATGACAGAATGCTTATGGACAAAAATGCTACAGCTTTGAGGCTAAATCATATGTCAAATAAAA

CTACTTCATCAAAAAACATGGAAATGGTCCAACAGAAAAAAGAGGGCCCCATTCCACCAGATGCACAAAA

TCCAGATATGTCGTTCTTTAAGATGCTATTCTTGCCAGAATCAGCAAGGTGGATACAAAGGACTCATGGA

AAGAACTCTCTGAACTCTGGGCAAGGCCCCAGTCCAAAGCAATTAGTATCCTTAGGACCAGAAAAATCTG

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TGGAAGGTCAGAATTTCTTGTCTGAGAAAAACAAAGTGGTAGTAGGAAAGGGTGAATTTACAAAGGACGT

AGGACTCAAAGAGATGGTTTTTCCAAGCAGCAGAAACCTATTTCTTACTAACTTGGATAATTTACATGAA

AATAATACACACAATCAAGAAAAAAAAATTCAGGAAGAAATAGAAAAGAAGGAAACATTAATCCAAGAGA

ATGTAGTTTTGCCTCAGATACATACAGTGACTGGCACTAAGAATTTCATGAAGAACCTTTTCTTACTGAG

CACTAGGCAAAATGTAGAAGGTTCATATGACGGGGCATATGCTCCAGTACTTCAAGATTTTAGGTCATTA

AATGATTCAACAAATAGAACAAAGAAACACACAGCTCATTTCTCAAAAAAAGGGGAGGAAGAAAACTTGG

AAGGCTTGGGAAATCAAACCAAGCAAATTGTAGAGAAATATGCATGCACCACAAGGATATCTCCTAATAC

AAGCCAGCAGAATTTTGTCACGCAACGTAGTAAGAGAGCTTTGAAACAATTCAGACTCCCACTAGAAGAA

ACAGAACTTGAAAAAAGGATAATTGTGGATGACACCTCAACCCAGTGGTCCAAAAACATGAAACATTTGA

CCCCGAGCACCCTCACACAGATAGACTACAATGAGAAGGAGAAAGGGGCCATTACTCAGTCTCCCTTATC

AGATTGCCTTACGAGGAGTCATAGCATCCCTCAAGCAAATAGATCTCCATTACCCATTGCAAAGGTATCA

TCATTTCCATCTATTAGACCTATATATCTGACCAGGGTCCTATTCCAAGACAACTCTTCTCATCTTCCAG

CAGCATCTTATAGAAAGAAAGATTCTGGGGTCCAAGAAAGCAGTCATTTCTTACAAGGAGCCAAAAAAAA

TAACCTTTCTTTAGCCATTCTAACCTTGGAGATGACTGGTGATCAAAGAGAGGTTGGCTCCCTGGGGACA

AGTGCCACAAATTCAGTCACATACAAGAAAGTTGAGAACACTGTTCTCCCGAAACCAGACTTGCCCAAAA

CATCTGGCAAAGTTGAATTGCTTCCAAAAGTTCACATTTATCAGAAGGACCTATTCCCTACGGAAACTAG

CAATGGGTCTCCTGGCCATCTGGATCTCGTGGAAGGGAGCCTTCTTCAGGGAACAGAGGGAGCGATTAAG

TGGAATGAAGCAAACAGACCTGGAAAAGTTCCCTTTCTGAGAGTAGCAACAGAAAGCTCTGCAAAGACTC

CCTCCAAGCTATTGGATCCTCTTGCTTGGGATAACCACTATGGTACTCAGATACCAAAAGAAGAGTGGAA

ATCCCAAGAGAAGTCACCAGAAAAAACAGCTTTTAAGAAAAAGGATACCATTTTGTCCCTGAACGCTTGT

GAAAGCAATCATGCAATAGCAGCAATAAATGAGGGACAAAATAAGCCCGAAATAGAAGTCACCTGGGCAA

AGCAAGGTAGGACTGAAAGGCTGTGCTCTCAAAACCCACCAGTCTTGAAACGCCATCAACGGGAAATAAC

TCGTACTACTCTTCAGTCAGATCAAGAGGAAATTGACTATGATGATACCATATCAGTTGAAATGAAGAAG

GAAGATTTTGACATTTATGATGAGGATGAAAATCAGAGCCCCCGCAGCTTTCAAAAGAAAACACGACACT

ATTTTATTGCTGCAGTGGAGAGGCTCTGGGATTATGGGATGAGTAGCTCCCCACATGTTCTAAGAAACAG

GGCTCAGAGTGGCAGTGTCCCTCAGTTCAAGAAAGTTGTTTTCCAGGAATTTACTGATGGCTCCTTTACT

CAGCCCTTATACCGTGGAGAACTAAATGAACATTTGGGACTCCTGGGGCCATATATAAGAGCAGAAGTTG

AAGATAATATCATGGTAACTTTCAGAAATCAGGCCTCTCGTCCCTATTCCTTCTATTCTAGCCTTATTTC

TTATGAGGAAGATCAGAGGCAAGGAGCAGAACCTAGAAAAAACTTTGTCAAGCCTAATGAAACCAAAACT

TACTTTTGGAAAGTGCAACATCATATGGCACCCACTAAAGATGAGTTTGACTGCAAAGCCTGGGCTTATT

TCTCTGATGTTGACCTGGAAAAAGATGTGCACTCAGGCCTGATTGGACCCCTTCTGGTCTGCCACACTAA

CACACTGAACCCTGCTCATGGGAGACAAGTGACAGTACAGGAATTTGCTCTGTTTTTCACCATCTTTGAT

GAGACCAAAAGCTGGTACTTCACTGAAAATATGGAAAGAAACTGCAGGGCTCCCTGCAATATCCAGATGG

AAGATCCCACTTTTAAAGAGAATTATCGCTTCCATGCAATCAATGGCTACATAATGGATACACTACCTGG

CTTAGTAATGGCTCAGGATCAAAGGATTCGATGGTATCTGCTCAGCATGGGCAGCAATGAAAACATCCAT

TCTATTCATTTCAGTGGACATGTGTTCACTGTACGAAAAAAAGAGGAGTATAAAATGGCACTGTACAATC

TCTATCCAGGTGTTTTTGAGACAGTGGAAATGTTACCATCCAAAGCTGGAATTTGGCGGGTGGAATGCCT

TATTGGCGAGCATCTACATGCTGGGATGAGCACACTTTTTCTGGTGTACAGCAATAAGTGTCAGACTCCC

CTGGGAATGGCTTCTGGACACATTAGAGATTTTCAGATTACAGCTTCAGGACAATATGGACAGTGGGCCC

CAAAGCTGGCCAGACTTCATTATTCCGGATCAATCAATGCCTGGAGCACCAAGGAGCCCTTTTCTTGGAT

CAAGGTGGATCTGTTGGCACCAATGATTATTCACGGCATCAAGACCCAGGGTGCCCGTCAGAAGTTCTCC

AGCCTCTACATCTCTCAGTTTATCATCATGTATAGTCTTGATGGGAAGAAGTGGCAGACTTATCGAGGAA

ATTCCACTGGAACCTTAATGGTCTTCTTTGGCAATGTGGATTCATCTGGGATAAAACACAATATTTTTAA

CCCTCCAATTATTGCTCGATACATCCGTTTGCACCCAACTCATTATAGCATTCGCAGCACTCTTCGCATG

GAGTTGATGGGCTGTGATTTAAATAGTTGCAGCATGCCATTGGGAATGGAGAGTAAAGCAATATCAGATG

CACAGATTACTGCTTCATCCTACTTTACCAATATGTTTGCCACCTGGTCTCCTTCAAAAGCTCGACTTCA

CCTCCAAGGGAGGAGTAATGCCTGGAGACCTCAGGTGAATAATCCAAAAGAGTGGCTGCAAGTGGACTTC

CAGAAGACAATGAAAGTCACAGGAGTAACTACTCAGGGAGTAAAATCTCTGCTTACCAGCATGTATGTGA

AGGAGTTCCTCATCTCCAGCAGTCAAGATGGCCATCAGTGGACTCTCTTTTTTCAGAATGGCAAAGTAAA

GGTTTTTCAGGGAAATCAAGACTCCTTCACACCTGTGGTGAACTCTCTAGACCCACCGTTACTGACTCGC

TACCTTCGAATTCACCCCCAGAGTTGGGTGCACCAGATTGCCCTGAGGATGGAGGTTCTGGGCTGCGAGG

CACAGGACCTCTACTGAGGGTGGCCACTGCAGCACCTGCCACTGCCGTCACCTCTCCCTCCTCAGCTCCA

GGGCAGTGTCCCTCCCTGGCTTGCCTTCTACCTTTGTGCTAAATCCTAGCAGACACTGCCTTGAAGCCTC

CTGAATTAACTATCATCAGTCCTGCATTTCTTTGGTGGGGGGCCAGGAGGGTGCATCCAATTTAACTTAA

CTCTTACCTATTTTCTGCAGCTGCTCCCAGATTACTCCTTCCTTCCAATATAACTAGGCAAAAAGAAGTG

AGGAGAAACCTGCATGAAAGCATTCTTCCCTGAAAAGTTAGGCCTCTCAGAGTCACCACTTCCTCTGTTG

TAGAAAAACTATGTGATGAAACTTTGAAAAAGATATTTATGATGTTAACATTTCAGGTTAAGCCTCATAC

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GTTTAAAATAAAACTCTCAGTTGTTTATTATCCTGATCAAGCATGGAACAAAGCATGTTTCAGGATCAGA

TCAATACAATCTTGGAGTCAAAAGGCAAATCATTTGGACAATCTGCAAAATGGAGAGAATACAATAACTA

CTACAGTAAAGTCTGTTTCTGCTTCCTTACACATAGATATAATTATGTTATTTAGTCATTATGAGGGGCA

CATTCTTATCTCCAAAACTAGCATTCTTAAACTGAGAATTATAGATGGGGTTCAAGAATCCCTAAGTCCC

CTGAAATTATATAAGGCATTCTGTATAAATGCAAATGTGCATTTTTCTGACGAGTGTCCATAGATATAAA

GCCATTTGGTCTTAATTCTGACCAATAAAAAAATAAGTCAGGAGGATGCAATTGTTGAAAGCTTTGAAAT

AAAATAACAATGTCTTCTTGAAATTTGTGATGGCCAAGAAAGAAAATGATGATGACATTAGGCTTCTAAA

GGACATACATTTAATATTTCTGTGGAAATATGAGGAAAATCCATGGTTATCTGAGATAGGAGATACAAAC

TTTGTAATTCTAATAATGCACTCAGTTTACTCTCTCCCTCTACTAATTTCCTGCTGAAAATAACACAACA

AAAATGTAACAGGGGAAATTATATACCGTGACTGAAAACTAGAGTCCTACTTACATAGTTGAAATATCAA

GGAGGTCAGAAGAAAATTGGACTGGTGAAAACAGAAAAAACACTCCAGTCTGCCATATCACCACACAATA

GGATCCCCCTTCTTGCCCTCCACCCCCATAAGATTGTGAAGGGTTTACTGCTCCTTCCATCTGCCTGACC

CCTTCACTATGACTACACAGAATCTCCTGATAGTAAAGGGGGCTGGAGACAAGGATAAGTTATAGAGCAG

TTGGAGGAAGCATCCAAAGATTGCAACCCAGGGCAAATGGAAAACAGGAGATCCTAATATGAAAGAAAAA

TGGATCCCAATCTGAGAAAAGGCAAAAGAATGGCTACTTTTTTCTATGCTGGAGTATTTTCTAATAATCC

TGCTTGACCCTTATCTGACCTCTTTGGAAACTATAACATAGCTGTCACAGTATAGTCACAATCCACAAAT

GATGCAGGTGCAAATGGTTTATAGCCCTGTGAAGTTCTTAAAGTTTAGAGGCTAACTTACAGAAATGAAT

AAGTTGTTTTGTTTTATAGCCCGGTAGAGGAGTTAACCCCAAAGGTGATATGGTTTTATTTCCTGTTATG

TTTAACTTGATAATCTTATTTTGGCATTCTTTTCCCATTGACTATATACATCTCTATTTCTCAAATGTTC

ATGGAACTAGCTCTTTTATTTTCCTGCTGGTTTCTTCAGTAATGAGTTAAATAAAACATTGACACATACA

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Problems 6-10: Consider the following sequences from a gene for nematode olfaction.

>odr-3 cDNA

ATGGGATTGTGTCAGAGCAGCTCAGAAGTTGATAAGGAACAACAGCAGCGAAATAAG

GAAATCGAAAAGCAGATTAACTCGGACAAACGATCAGCATCGAGTATCGTCAAACTT

CTTTTGTTAGGAGCTGGAGAATGCGGAAAATCGACAGTTTTGAAGCAGATGCAAATTC

TGCATTCCAATGGATTCACGGAAGAGGAAGTGAATGAGAAACGAGGAATCGTTTTCA

ATAACACGGTCACATCAATGTGTTCCATACTCCGAGCAATGGATGCTGTACTTCACAT

TCAATTAGAGAACTCCGAATTGGAGCCCGAGAAAGCTATGATTCTCAAGATTCAAGA

GAAGGGAGAAGAATCGGAACAGATGACACCTGAAAAACGAGATGCCCTGCTCGCATT

GTGGCAGGATAAAGGAGTGAAGAAAGCATATGAAATGAGGAGTGAATACCAACTCAT

CGACTCTGCTGCATTGTACGTTTCCCCTCTTCATACATCAAGATCTTCTATGATGCGTG

CTATTCCCTCCCTATCACTGCAGCCTAATGAGTACTTCCCCTTCAGTTTCCTCGATAAT

GTTCAACGCATCGCTGAGCCTGGATTCCGACCGACTGAGCAAGATATTCTCTTCTCGC

GAGTGGCCACAACCGGAGTCGTCGAAGTGAAATTCAAAATCAAGGAACTCGATTTCA

GAGTATTCGACGTAGGAGGGCAACGATCGGAGAGAAGAAAGTGGATTCACTGTTTCG

ATAACGTCGAATCAATCATTTTCATCACTGCCATCTCTGAATACGATCAAGTTCTTTTC

GAGGATGAAAGCACAAATCGTATGATCGAATCGATGCAACTCTTCAATTCCATCTGCA

ATTCTTCATGGTTTTTGTCTACTGCTATGATTCTCTTCATGAATAAGAAGGATCTCTTC

ATGGAGAAGATCAAACGCGTAAACATCACAACCGCCTTCCCTGATTATGAAGGAGGG

CAAAACTACGAAGAAGCAGTGAATTTCATCAAGCAAAAGTTCGCTGAACTAAATATG

AACCCTGATAAGAAGACGATTTACATGCACGAGACTTGCGCCACGGACACTAATCAG

GTTCAACTGGTCATTTCCTCTGTCATCGATACGATTATCCAGAAGAATCTACAGAAGG

CAGGAATGATGTAG

>odr-3 gDNA

ATGGGATTGTGTCAGAGCAGCTCAGAAGTTGATAAGGAACAACAGGTATTTCAATTAT

TCATCACATCAATCTCGTAATATCGTCGTCCCCATCTCTTCACCCACCCAAGTGCATTG

AATCTAATCATCCTCAATTTCAGCAGCGAAATAAGGAAATCGAAAAGCAGATTAACT

CGGACAAACGATCAGCATCGAGTATCGTCAAACTTCTTTTGTTAGGTAAGATCACAGA

TTGAAAGATCAAAGATCAATCCACTTATTTTCAGGAGCTGGAGAATGCGGAAAATCG

ACAGTTTTGAAGCAGATGCAGTGAGTTTTTGGGAACTTCAACTTTGAGCTTATGAGAG

AAGACAGATGTTTCGATCTGACAGAGAACAGAGGAGAACATTTTAGACATGAACTAT

TTCAGAATTCTGCATTCCAATGGATTCACGGAAGAGGAAGTGAATGAGAAACGAGGA

ATCGTTTTCAATAACACGGTGAGATTATCGATCAAGGGGATGTCATGACTAATCAATT

CATTACAGGTCACATCAATGTGTTCCATACTCCGAGCAATGGATGCTGTACTTCACATT

CAATTAGAGAACTCCGAATTGGAGGTCGGTTTATGATCTCCCTTTATCACACCTTCAA

ATAGTGTATGAATTGTATTGCAATCAAGGAACTGTTCCATGAACCTTTTTGTATCTCCC

ATTAAATTCATTGGGATCCGTGTTGATAGTGAATCTCAATCTCATGTGTATCGTGTAGT

CAGGGGCAACTGAGAAGGGGGGAAAGAAACGACGGGGTATTCATGAGATCCAGAAG

GGGATTGAGAGGGGGACTGAGTAGGAGTGAGAAGGGAGAGGGGGGAGGGGACGGG

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GACGGCTGTAATGGGAAAGATATAGGACGATCGACGTCAATAATCGTTGAAATTTGA

ACATGGGACCGACTCTGAAAAGGAACGATTTCAGCCCGAGAAAGCTATGATTCTCAA

GATTCAAGAGAAGGGAGAAGAATCGGAACAGATGACACCTGAAAAACGGTGAGTGA

CGACGATCGATTGATGATTCACCCATAACTGTGATTGCAGAGATGCCCTGCTCGCATT

GTGGCAGGATAAAGGAGTGAAGAAAGCATATGAAATGAGGAGTGAATACCAACTCAT

CGACTCTGCTGCATTGTACGTTTCCCCTCTTCATACATCAAGATCTTCTATGATGCGTG

CTATTCCCTCCCTATCACTGCAGCCTAATGAGTACTTCCCCTTCAGTTTCCTCGATAAT

GTTCAACGCATCGCTGAGCCTGGATTCCGACCGACTGAGCAAGATATTCTCTTCTCGC

GAGTGGCCACAACCGGAGTCGTCGAAGTGAAATTCAAAATCAAGGAACTCGATTTCA

GGTCGGAATTAATGATTGATGGAGGCTGTTTATAGGAAATTGTCTGGAGTTGCTTTTC

TTATCACTTGTTATTCCTTCGTTTTCCCATTTCTCGTCGTGTGAAGAAGGAGACGATAT

CGTCATAATGGATTAGTATCGATTGAGGGAGAGGGAAAGGGGGAGGGGGGAGGGGG

GGAGGCACTCGAAGAGAAGCTAATGAAGTCCACGGGAAGAAGGGATTTACACTAGA

AGAGACTGACTACAGAATGGAGGCTAGAGATTAAACTGATGAGAGATTATTATGAAT

ACAATTACAATAACACACAATTATGAGTATTCTTAGATTGGTATTGTGCATCAGACAT

CTAGGAAATGGATTAATACTCATTCAATGCTTGCATCTCTTCAGAGTATTCGACGTAG

GAGGGCAACGATCGGAGAGAAGAAAGTGGATTCACTGTTTCGATAACGTCGAATCAA

TCATTTTCATCACTGCCATCTCTGAATACGATCAAGTTCTTTTCGAGGATGAAAGCACA

GTGAGAATTTACCCCCTTTGAGATCAATACAGTTCGAAATTCGGGTGATCGTCTCTTC

ATTTCAAATGATCTCTCATTAGTCAGTGCGACGAGAGAGTGTGTAAAATGAAGAGAC

GATTGTTCGAAGGTCGACCTCTAGAGATCATCGATCATTATTGCGTATGAGACAACGA

GTCATACATTCAATTCAATTTGAACAGAAACAGACATCGAAAGCACTTGAGGCACTTT

ACTTATTTTTGCGCAGAAAAAGTAGTTTCTCGTGATCATAATTGGCTATATTTTGAGCG

TTCTGGTGCATGAACTAATAGTACCCTATGTGATCAGTTTCATTCAGAATCGTATGATC

GAATCGATGCAACTCTTCAATTCCATCTGCAATTCTTCATGGTTTTTGTCTACTGCTAT

GATTCTCTTCATGAATAAGAAGGTAATTAATCTCCCCCTGTGATCTAACTGTTCATGAG

TCTGTAACATATTAGCACAACACTATCCATCTCATTCCCATGTTTTCATCTCATTACAT

CTATCGTATCAATAATCTCTTCGTCTGGGAGATATCTCTCTTTCTCTGATATTTTTGGAC

TCTCTTCAAATCACACGATCTCTTGTCTAATCTTCGCTTCATTTGAGGATCTCTTCATG

GAGAAGATCAAACGCGTAAACATCACAACCGCCTTCCCTGATTATGAAGGTGAGAAG

GGAGAAGGGAAAGAGAAGGAAGACGGAGGGGGAGATGGGGGATCGACATCGATGG

ATTAAGTGGGCGATAATGCACTACAAGAGAATGACGTAGACTGATCATCATCAAGAT

TGAAATAACTGTTGTCTTTCCCGTAGACGATTATTCCCATCCCACACTATTTCTCATTT

GATCAAGTTCATTCTCAGGAGGGCAAAACTACGAAGAAGCAGTGAATTTCATCAAGC

AAAAGTTCGCTGAACTAAATATGAACCCTGATAAGAAGACGATTTACATGCACGAGA

CTTGCGCCACGGACACTAATCAGGTTAGACACATCGAGAGATTCGCAGTTATTTACTC

GCATTCCAAATGTTTGACTCTTGTGTAGTAGGCAACACACCACTTGCAATATCTATTG

AAGGAATATTGAACATTCTCCTAATTGCTTCGCTTGATTTTGTAATCAAAACACTTGTT

ATTCATAATTAAAACAAGCTGAATAGGATACCAATGAACACACTCACCTACCGTATCC

TTCAGGTTCAACTGGTCATTTCCTCTGTCATCGATACGATTATCCAGAAGAATCTACAG

AAGGCAGGAATGATGTAGACACTTCTCCTTTCTTCTTCAGAGGAATCCAATGATTTTTC

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AAACGAGCTTTTCAAACTCAATATTGATCGATATTTGCTCATTCACGTGTTCTCTTCTC

TTCTTATTCATCTCGTCCATAATATTTTGATCTCACTAATCGATAGTGTAGTCACGTCA

CTTACCGTACTACCGTTAGATCAAATTGAAATGTGATTTTGCTATATTTCGTGTACCCT

TACAAAACTTCGATCTCGATCCTTCCAAAAAGGAACAAATGTTCTCAATCCTCATGTA

TCCCTCCAACATTCTCCCCATTTGTCAAATGCATCTTTCTTTATAATGCATTTCGACCCA

TATCTCCATTCTCGATTGTTGTTTTTGCCTGCTAAATTCTGAATTCCCATCATAAATCTC

ACGTAGGTTTGATACTCTCGTCACATCGATTGCTGCGCATTCAGAGAAACCGAGTGAT

AAACAATTTTTATGAGATTTGGAGAGTGCGAAAAGAGTAACAATGAAATCAACAACG

AGAACAATCAATGCAGTTTGAGGTCTATTGTGGGACTAACTAACTGCTAACAATTAAT

GAGGGGAGGTTAGACCCGAAATGGAGTTGGGTGAGGAATTAGGCAGTTACTGATGAT

TAGGCGGTCTCCATAGATTCAATTACCTGAAATAAGAATGTCAATAATATAGAGATAT

ATACACTGATGCGATTGTACTTGTTCTATCACGATATTCCACAAGCTTCTTTTAGGAAA

GAGAAACTAGAGAAGAAATCACGCATTACATGAAAATATTGCAAACCTTATAGTAAG

GGCTGTTCTTGTATATTCTAATCTTCTTCTCGAGTTGCTTGGGAGTCTCTTGCCACAGA

TTGCGAGGATCGCGAGACATCGCCTGACATGTTAGAATTTTAGAAAACGATTATTTCC

ATAACCATAAATCCAGCATCAAATGTTACAGGAAGTTACATGTGAGAGGATAGTAAC

CTTAGTCGCCACCGTGGTTATTATCCTCATACATGTCTTTCATTTGAAATACATCTCAT

TTAAAAGACTGATAAGGTGACTTTTATAGTATTCGATTGTATGTAAATAAAATAACGA

ATAAATATAACCTTGTAGTCATCTTTGTGCTTCTCATACATGTATACGCAGAACTCGAT

ATCTCTCTCCTGGAGACGGAATTCGCGGCCAACCTGATAAAATCAACCAATGAAAAA

AAGACATTTTTGAAGGTAACAGATAGGACCGAATGA

6. How many exons does this coding region contain?

7. How many potential polyA consensus sequence ‘AATAAA’ sites are there?

8. You found a mutation in this gene (see below). Which part of the gene is the

mutation in (exon, intron, 5’ UTR, 3’ UTR)?

AGATGCCCTGCTCGCATTGTGGCAGGATAAAGGA_TGAAGAAAGCATATGAAA

TGAGGAGTGAATACCAACTCATCGACTCTGCTGCATTGTACGTTTCCCCTCTTC

ATACATCAAGATCTTCTATGATGCGTGCTATTCCCTCCCTATCACTGCAGCCTA

ATGAGTACTTCCCCTTCAGTTTCCTCGATAATGTTCAACGCATCGCTGAGCCTG

GATTCCGACCGACTGAGCAAGATATTCTCTTCTCGCGAGTGGCCACAACCGGA

GTCGTCGAAGTGAAATTCAAAATCAAGGAACTCGATTTCAG

9. How would this mutation affect the protein sequence?

Hatan Algow

Hong BIOL 360 Sp 2021

10. Using BamHI and PstI, what is the length of the odr-3 genomic DNA fragment will

be that will be cut? Can the fragment be cloned into the MCS of pUC19?

Hatan Algow