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AxiChrom™ 300-1600 columns Operating Instructions Original instructions

Table of Contents 51 Introduction .......................................................................................................... 61.1 About this manual ................................................................................................................................ 71.2 Important user information ............................................................................................................. 91.3 Regulatory information ......................................................................................................................

131.4 Associated documentation ..............................................................................................................

152 Safety instructions ............................................................................................... 162.1 Safety precautions ............................................................................................................................... 312.2 Labels ......................................................................................................................................................... 352.3 Emergency procedures ...................................................................................................................... 382.4 Safety zones and access areas ...................................................................................................... 402.5 Crush injury risks ................................................................................................................................... 442.6 Recycling information ......................................................................................................................... 452.7 Declaration of Hazardous Substances (DoHS) ........................................................................

473 System description .............................................................................................. 483.1 Column description .............................................................................................................................. 523.2 AxiChrom 300-1600 Master description ....................................................................................

554 Installation ............................................................................................................ 564.1 Site requirements .................................................................................................................................. 584.2 Transport .................................................................................................................................................. 624.3 Unpacking ................................................................................................................................................ 634.4 Wheel kits ................................................................................................................................................. 654.5 Leveling the column ............................................................................................................................ 684.6 Connections ............................................................................................................................................

805 Operation .............................................................................................................. 815.1 AxiChrom 300-1600 Master and UNICORNsoftware ........................................................... 825.2 Starting up and shutting down the AxiChrom 300-1600 Master ................................... 885.3 Cleaning a new column ..................................................................................................................... 905.4 Performing a leakage test ................................................................................................................ 955.5 Preparing slurry and buffer .............................................................................................................. 965.6 Priming the column .............................................................................................................................

1025.7 Packing the column ............................................................................................................................. 1045.8 Cleaning a packed column .............................................................................................................. 1055.9 Unpacking the column .......................................................................................................................

1066 General maintenance and service .................................................................... 1076.1 Service and preventive maintenance ......................................................................................... 1086.1.1 Service recommendations ......................................................................................................... 1096.1.2 Cleaning before maintenance or service ............................................................................. 1106.1.3 Maintenance schedules .............................................................................................................. 1116.2 General maintenance tools .............................................................................................................

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1136.3 Power failure during maintenance ............................................................................................... 1156.4 Putting the column in and out of maintenance mode ........................................................ 1426.5 Media valve maintenance ................................................................................................................ 1556.6 Changing liquid in the flushing channels .................................................................................. 1586.7 Changing flushing connector O-rings ......................................................................................... 1606.8 Leakage test ............................................................................................................................................

1617 Maintenance of 300-1000 columns .................................................................. 1627.1 Maintenance tools for 300-1000 columns ............................................................................... 1637.2 Maintaining the adapter inlet/outlet ........................................................................................... 1667.3 Maintaining columns with stainless steel bed supports .................................................... 1687.3.1 Disassembling the bottom assembly .................................................................................... 1707.3.2 Disassembling the adapter ........................................................................................................ 1757.3.3 Assembling the adapter .............................................................................................................. 1797.3.4 Assembling the bottom assembly .......................................................................................... 1817.4 Maintaining columns with plastic bed supports .................................................................... 1837.4.1 Disassembling the bottom assembly .................................................................................... 1857.4.2 Disassembling the adapter ........................................................................................................ 1917.4.3 Assembling the adapter .............................................................................................................. 1977.4.4 Assembling the bottom assembly ..........................................................................................

1988 Maintenance of 1200-1600 columns ................................................................ 1998.1 Maintenance tools for 1200-1600 columns ............................................................................. 2078.2 Rinse nozzle maintenance ................................................................................................................ 2158.3 Lifting and lowering the bottom backing plate ...................................................................... 2278.4 Maintaining columns with stainless steel bed supports .................................................... 2308.4.1 Disassembling the bottom assembly .................................................................................... 2388.4.2 Disassembling the adapter ........................................................................................................ 2518.4.3 Assembling the adapter .............................................................................................................. 2628.4.4 Assembling the bottom assembly .......................................................................................... 2688.5 Maintaining columns with plastic bed supports .................................................................... 2718.5.1 Disassembling the bottom assembly .................................................................................... 2748.5.2 Disassembling the adapter ........................................................................................................ 2848.5.3 Assembling the adapter .............................................................................................................. 2928.5.4 Assembling the bottom assembly ..........................................................................................

2959 Master maintenance ........................................................................................... 2969.1 AxiChrom 300-1600 Master firmware installation ................................................................ 2979.2 Updating AxiChrom 300-1600 Master firmware ....................................................................

30010 Cleaning and storage .......................................................................................... 30110.1 Cleaning an empty column .............................................................................................................. 30310.2 Cleaning stainless steel bed supports ........................................................................................ 30410.3 Storage ......................................................................................................................................................

30611 Troubleshooting ...................................................................................................

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31112 Reference information ........................................................................................ 31212.1 Specifications ......................................................................................................................................... 31412.2 Weights and dimensions ................................................................................................................... 32012.3 Spare parts and accessories ........................................................................................................... 32112.4 Chemical resistance ............................................................................................................................ 32412.4.1 Sodium chloride and strong pH solutions ........................................................................... 32512.5 System recommendations ............................................................................................................... 32612.6 Health and Safety Declaration Form ........................................................................................... 32812.7 Ordering information ..........................................................................................................................

329Index .......................................................................................................................

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Table of Contents

1 Introduction

About this chapter This chapter contains important user information, description of safety notices, regula- tory information, the intended use of AxiChrom columns and information about additional documentation.

In this chapter This chapter contains the following sections:

See pageSection

61.1 About this manual

71.2 Important user information

91.3 Regulatory information

131.4 Associated documentation

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 5

1 Introduction

1.1 About this manual

Purpose of this manual The Operating Instructions provide you with the information needed to install, operate and maintain the product in a safe way.

Scope of this manual This manual describes the basic components and principles of operation of AxiChrom 300-1600 columns using an AxiChrom 300-1600 Master and associated equipment. For detailed information about each individual product, refer to the relevant documentation provided with each column, or contact your local GE representative.

For information about the principles of operation of AxiChrom 300-1000 columns using anAxiChrom300-1000Master and associated equipment, refer to AxiChrom 300-1000 columns Operating Instructions 28-9562-90 and AxiChrom 300-1000 columns User Manual 28-9562-89, or contact your local GE representative.

Note:

Typographical conventions Software items are identified in the text by bold italic text. A colon separates menu levels, thus File:Open refers to the Open command in the File menu.

Hardware items are identified in the text by bold text (for example, Power).

Part annotations in the descriptive illustrations and tables in this document are not in- tended to represent the part number designations used when ordering spare parts.

Refer to the appropriate column documentation, such as the Material Conformity and Spare Parts list when ordering replacement parts, see Section 12.7 Ordering information, on page 328.

6 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

1 Introduction 1.1 About this manual

1.2 Important user information

Read this before operating the product

All users must read the entire Operating Instructions before installing, operating or maintaining the product.

Always keep the Operating Instructions at hand when operating the product.

Do not operate the product in any other way than described in the user documentation. If you do, you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment.

Intended use The AxiChrom family of process columns has been designed for low pressure chromato- graphic separation of biomolecules such as proteins, peptides and oligonucleotides in GMP-regulated environments. AxiChrom columns are intended for production use only and should not be used for diagnostic purposes in any clinical or in vitro procedures.

The columns are not suitable for operation in a potentially explosive atmosphere or for handling flammable liquids. If the columns are used for purposes other than those specified in the user documentation, safe operation and the protection provided by the system may be impaired.

The AxiChrom Master is a self-contained operator console featuring interactive guides (wizards) for work procedures such as packing, unpacking and maintenance. The user interface is a touch screen panel. Using the AxiChrom Master the operator has control over the workflow, and can use manual control to steer adapter movement and open or close the Media valve. The AxiChrom 300-1600 Master can also be used to control a slurry tank valve and is equipped with a pneumatic Rinse nozzle control (AxiChrom 1200- 1600 columns only).

One AxiChrom Master unit can be used to control several columns (one at a time). The AxiChrom Master can also be connected to an ÄKTAprocess™ system, which enables the column to be controlled using the UNICORN™ software of the ÄKTAprocess.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 7

1 Introduction 1.2 Important user information

Prerequisites In order to operate the equipment in the way it is intended, the following prerequisites must be fulfilled:

• You must read and understand the safety instructions in the user documentation.

• You shall have a thorough knowledge of the entire system and process.

• All operations must be performed by qualified personnel who are adequately trained.

Safety notices This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product. See definitions below.

WARNING WARNING indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.

CAUTION CAUTION indicates a hazardous situation which, if not avoided, could result in minor or moderate injury. It is important not to pro- ceed until all stated conditions are met and clearly understood.

NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment.

Notes and tips A note is used to indicate information that is important for trouble-free and optimal use of the product.

Note:

A tip contains useful information that can improve or optimize your procedures.Tip:

8 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

1 Introduction 1.2 Important user information

1.3 Regulatory information

This section describes the directives and standards that are fulfilled by AxiChrom columns and the AxiChrom Master.

Manufacturing information The table below summarizes the required manufacturing information. For further infor- mation, see the EU Declaration of Conformity (DoC) document.

ContentRequirement

GE Healthcare Bio-Sciences AB,Name and address of manufacturer

Björkgatan 30, SE 751 84 Uppsala, Sweden

Conformity with EU directives The AxiChrom column and AxiChrom Master comply with the European directives listed in the table. A copy of the EU Declaration of Conformity is supplied with each product.

TitleDirective

Machinery Directive (MD)2006/42/EC

ElectroMagnetic Compatibility (EMC) Directive2004/108/EC

AxiChrom columns also comply with, but are not CE marked with, the European Pressure Equipment Directive (PED) 97/23/EC.

International standards The AxiChrom column and AxiChrom Master are designed in accordance with the re- quirements of the following standards and regulations:

AxiChrom columns are designed using the guidelines contained in PM-4.4, “Chromatography Columns”, of the ASMEBioprocessing Equipment Standard.

Note:

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 9

1 Introduction 1.3 Regulatory information

NotesDescriptionStandard

EN standard is harmonized with EU directive 2006/95/EC

Safety requirements for electrical equip- ment for measurement, control, and lab- oratory use

EN/IEC 61010-1, UL 61010-1, CAN/CSA C22.2 No 61010-1

Applies only to the AxiChrom Master

Applies only to the column

Safety of machinery – Electrical equip- ment of machines – Part 1: General re- quirements

EN 60204-1

EN standard is harmonized with EU directive 2004/108/EC

EMC emissions and immunity require- ments for electrical equipment for mea- surement, control and laboratory use

EN 61326-1 (Emis- sion according to CISPR 11, Group 1, class A)

EN ISO standard is harmonized with EU directive 2006/42/EC

Safety of machinery, general principles for design, risk assessment and risk reduc- tion

EN ISO 12100

Applies only to stainless steel columns

Boiler and Pressure Vessel Code (BPVC)ASME BPVC VIII, div 1

Pressure Vessels for Human OccupancyASME PVHO-1

Applies only to the AxiChrom Master

UL standard for Safety for Industrial Control Panels

UL 508A

10 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

1 Introduction 1.3 Regulatory information

CE marking

The CE marking and the corresponding EU Declaration of Conformity is valid for the in- strument when it is:

• used as a stand-alone unit, or

• connected to other products recommended or described in the user documentation, and

• used in the same state as it was delivered from GE, except for alterations described in the user documentation.

UL conformity

The above symbol appears on equipment that is UL508A compliant. The symbol can be found on the inside of the door of the electrical cabinet at the rear of the AxiChrom 300-1600 Master.

FCC compliance This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

The user is cautioned that any changes ormodifications not expressly approved by GE could void the user’s authority to operate the equipment.

Note:

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 11

1 Introduction 1.3 Regulatory information

This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea- sonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequen- cy energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be re- quired to correct the interference at his own expense.

Environmental conformity This product conforms to the following environmental requirements.

TitleRequirement

Restriction of Hazardous Substances (RoHS) Directive2011/65/EU

Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU

Administration on the Control of Pollution Caused by Electronic Information Products, China Restriction of Hazardous Sub- stances (RoHS)

ACPEIP

Registration, Evaluation, Authorization and restriction of CHemicals (REACH)

Regulation (EC) No 1907/2006

Regulatory compliance of connected equipment

Any electrical equipment connected to the AxiChrom column and AxiChrom 300-1600 Master should meet the safety requirements of EN/IEC 61010-1, EN/IEC 60204-1 or other relevant standards. Within the EU connected equipment must be CE marked.

Additional regulatorycompliance All wetted polymer and elastomeric parts are animal origin-free or comply with the conditions in the EMA/410/01 CPMP Note standard, and are also classified according to USP Class VI, 21 CFR Part 177.

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1 Introduction 1.3 Regulatory information

1.4 Associated documentation The documentation package supplied with AxiChrom includes product documentation binders that contain detailed specifications, drawings and traceability documents for the individual AxiChrom column and AxiChrom Master.

AxiChrom instruction manuals The following table lists examples of the user instructions that are provided with AxiChrom columns and the AxiChrom Master.

The manual edition provided with an individual AxiChrom product depends on the AxiChrom Master type and column size.

Note:

DescriptionManual ID number

User documentation

Column installation site requirements. Configured specifically for each individual column.

Column-specificAxiChrom columns Site Preparation Guide

Transport and unpacking instructions for use with AxiChrom 300-1000 columns until 2015.

28956291AxiChrom Read Me First

For use with AxiChrom 300-1000 columns from 2015 onwards.

29141868AxiChrom 300-1000 columns Unpacking Instructions

For use with AxiChrom 1200-1600 columns.29061719AxiChrom 1200-1600 columns Unpacking Instruc- tions

For use with AxiChrom 300-1600 Master.29110991AxiChrom 300-1600 Master Unpacking Instructions

For use with AxiChrom 300-1000 Master operated columns.

28956290AxiChrom 300-1000 columns Operating Instructions

For use with AxiChrom 300-1600 Master operated columns.

29065430AxiChrom 300-1600 columns Operating Instructions

For use with AxiChrom 300-1000 Master operated columns.

28956289AxiChrom 300-1000 columns User Manual

For use with AxiChrom 300-1600 Master operated columns.

29099939AxiChrom 300-1600 columns User Manual

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 13

1 Introduction 1.4 Associated documentation

Component documentation Documentation for components produced both by GE and by a third-party are, if existent, also included in the document package.

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1 Introduction 1.4 Associated documentation

2 Safety instructions

About this chapter This chapter describes safety precautions, safety labels, emergency procedures and decommissioning information for AxiChrom columns.

In this chapter This chapter contains the following sections:

See pageSection

162.1 Safety precautions

312.2 Labels

352.3 Emergency procedures

382.4 Safety zones and access areas

402.5 Crush injury risks

442.6 Recycling information

452.7 Declaration of Hazardous Substances (DoHS)

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 15

2 Safety instructions

2.1 Safety precautions

Introduction Before installing, operating or maintaining the AxiChrom column, you must be aware of the hazards described in this manual. Follow the instructions provided to avoid personal injury or damage to the equipment.

The warnings and cautions in the user documentation shall in no way take precedence over more restrictive local regulations and policies.

For your personal safety it is important that you have a proper knowledge of the entire system that the column is part of. Study any complementary safety instructions and use appropriate personal protection equipment for the specific application and operation environment.

Risk assessment AxiChrom columns have been designed and manufactured to provide a high level of personal safety. However, the residual risk is highly dependent on the application and environment in which the column is operated. In order to determine the safe operation of the equipment a risk assessment must be made. This risk assessment, in combination with local regulations and policies, will result in specific safety instructions for installation, operation and maintenance, use of proper personal protective equipment, or other ar- rangements that are needed to operate your process safely.

General precautions

WARNING The customer must make sure that all installation, maintenance, operation and inspection is carried out by qualified personnel who are adequately trained, understand and adhere to local regulations and the operating instructions, and have a thorough knowledge of AxiChrom columns and the entire process.

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2 Safety instructions 2.1 Safety precautions

WARNING The product must NOT be used for any other purposes than chro- matographic separations. The equipment must not be used as:

• Storage tanks for chemicals etc.

• General pressure vessels

• Fermentation vessels

• Gas storage tanks

The product must never contain air or gas under pressure.

CAUTION To prevent bacterial growth, the product may be partly filled with denatured alcohol (18% C2H5OH (ethanol), 2% C3H7OH (isopropanol) and 80% H2O (water)) at delivery.

The denatured alcohol mixture can be hazardous to humans if consumed.

Flush out the denatured alcohol before assembling, testing or inte- grating the product into the intended process context.

Flammable liquids and explosive environments

WARNING Flammable liquids. This product is not approved to handle flammable liquids.

WARNING Explosive environment. The products are not approved for work in a potentially explosive atmosphere, in areas classified as Zone 0 to Zone 2 according to IEC 60079-10 2002. The products do not fulfill the requirements of the ATEX Directive.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 17

2 Safety instructions 2.1 Safety precautions

WARNING Some of the chemicals used with AxiChrom columns may be flammable under certain conditions. Make sure to use chemicals only under conditions where they are not flammable. Refer to local and/or national classifications of flammable liquids.

Personal protection

WARNING For personal safety during the transportation, installation, operation, maintenance and service of columns, use protective glasses and other personal protective equipment appropriate for the current application at all times. The following personal protective equipment should always be available:

• Protective glasses

• Working gloves to protect against sharp edges

• Protective footwear, preferably with steel toe-cap

• Disposable gloves

Always use clean disposable gloves when manually handling parts.

WARNING Hazardous substances. When using hazardous chemicals, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the substances used. Follow local and/or national regulations for safe operation and maintenance of the product.

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2 Safety instructions 2.1 Safety precautions

Installing and moving the columns and Master

WARNING Packing crates and columns shall only be moved by personnel with appropriate training, and in adherence with local regulations.

Even if the instructions in the AxiChrom user documentation are followed, it is the responsibility of the customer to guarantee the safety of personnel while working with the column.

WARNING Due to the significant weight of AxiChrom columns, suitable equipment with adequate lifting capacity must be used when moving the unit or performing maintenance procedures.

• Make sure that all lifting equipment has an adequate capacity to safely lift the weight of the crate and column.

• Make sure that equipment used to move crates or columns over a sloping floor has adequate braking power to control the momentum and prevent the crate or column from moving too fast.

WARNING Tipping risk.When lifting AxiChrom crates or columns using a lifting truck, make sure that the crate or column is positioned so that there is no risk of the lifting equipment tipping over.

The length of the lifting forks and the lifting capacity at the tips of the forks of the lifting equipment must allow lifting the weight of the crate and column without the risk of tipping off the forks.

WARNING

AxiChrom crates and columns are marked with center of gravity symbols. Make sure that center of gravity symbols are properly balanced and centered with re- spect to the lifting equipment used, so that the crate or column will not accidentally tip when moved.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 19

2 Safety instructions 2.1 Safety precautions

WARNING Make sure never to place any body part between the column stand and a wheel kit. Always use the handle to hold a trolley when fitting it under the column stand.

WARNING Maintain a safe distance when moving the column. Make sure that no person is near the column when the column is in motion, other than the personnel required to perform lifting and moving procedures.

WARNING Columns in swing-out or any kind of maintenance mode must not be moved. This may cause personal injury or damage to the column.

WARNING Make sure that the wheels are immobilized when fitting a wheel kit, so that the column cannot begin to roll away.

CAUTION Packing crates may have been exposed to pesticides, depending on the regulations of the country of delivery. Recycle packing crates according to local recommendations for pesticide-treated wood.

NOTICE When lifting an AxiChrom column with a lifting truck or similar equipment, lift the column by the long sides. Do not lift the column from the front or back (short sides).

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2 Safety instructions 2.1 Safety precautions

NOTICE Disconnect all tubing, hoses and cables before moving the column.

NOTICE Perform all assembly and handling of material on clean, non-ferritic surfaces.

NOTICE Make sure not to damage the surface of the column during unpack- ing and moving. Use padding material to protect column contact surfaces.

Power supply

WARNING All electrical installations must be performed by authorized person- nel only.

WARNING The EMERGENCY STOP button will not shut off the electrical power to the motor of the column. Use the Power Switch on the AxiChrom Master connector panel to shut off the electrical power.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 21

2 Safety instructions 2.1 Safety precautions

WARNING Do not block access to the EMERGENCY STOP button or the Power Switch on the AxiChrom Master. Position the AxiChrom Master close to the column in such a way that the EMERGENCY STOP button and thePower Switch can always be reached when working with the column.

WARNING To avoid the risk of electrocution, make sure that the power cable to the Master is disconnected or that the power is shut off at the outlet whenever maintenance work is performed on the AxiChrom Master. There is still electrical voltage present in the AxiChrom Master when it is shut off using the Power Switch.

WARNING Only properly trained personnel who have read and understood theOperating Instructionsare allowed to open the AxiChrom Master cabinet.

WARNING Always connect the AxiChrom Master to a power supply with pro- tective grounding.

WARNING Connection to the wrong power supply voltage may cause injury to personnel and damage to the equipment.

WARNING Power cables and connectors must be replaced or repaired by properly trained personnel, authorized by GE.

22 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.1 Safety precautions

WARNING A disconnecting system power switch must be installed, such as a branch circuit protection and disconnect switch installed at your facility. The system power switch should be installed at the wall to cut the power to the AxiChrom Master. Always use both the Master Power Switch (located on the back of the Master unit) and the system power switch for safe disconnection of the AxiChrom Master.

Operation

WARNING The working pressure of the column should never exceed 4 bar, otherwise there is a risk of personal injury and damage to the col- umn. Use pressure safety equipment, such as a pressure gauge, pressure relief valve, rupture disc or other pressure safety equip- ment to make sure that the maximum operating pressure of the column is not exceeded.

When rupture discs are used, make sure that these are positioned so that venting after rupture is directed to waste and not towards an area where personnel could be endangered.

WARNING Pressure safety equipment, such as a pressure relief valve or rup- ture disc must be installed at both of the mobile phases, before the column is used.

WARNING The column may still be pressurized when the process has been stopped using the EMERGENCY STOP button. Make sure that the pressure is released before disconnecting any tubing.

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2 Safety instructions 2.1 Safety precautions

CAUTION Before priming the column, make sure that tubing is connected to all rinse nozzle inlet/outlets and led to waste.

CAUTION When working with 20% v/v 1-propanol solutions:

• work in a well-ventilated area

• wear appropriate personal protective equipment

• keep storage containers tightly closed

CAUTION When unpacking chromatography media from the column, check the integrity of all connections and make sure that the collection vessels can accommodate the volumes involved.

NOTICE Always open and close external valves slowly. Sudden changes of pressure in combination with air in the column might cause damage to the column.

NOTICE Inspect all connections and tubing before use and replace any defective parts. Also inspect the column tube thoroughly before use to verify that there are no cracks or other visible signs of damage.

NOTICE Use particle-free water to prime the column. Do not use solutions containing ethanol or sodium hydroxide for column priming.

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2 Safety instructions 2.1 Safety precautions

NOTICE Make sure that any buffers used with the column are particle-free (down to 1 μm), as particles can block and damage the bed support.

NOTICE Acrylic column tubes have limited resistance to organic solvents. Wetted parts of the product may also be damaged by chemicals not listed in the Chemical Resistance information. Contact your GE representative before using chemicals that are not listed.

NOTICE Chlorides and low pH can cause corrosion of stainless steel. Rinse thoroughly with clean water after use.

Inspect the column regularly for signs of corrosive attacks, which may cause column damage if untreated. Note that the stainless steel bed supports are especially vulnerable to corrosion.

NOTICE If there is air in the column, make sure that there is an unrestricted flow path for evacuation through an open valve before the adapter is moved or liquid is pumped into the column.

NOTICE Make sure that the pressure does not exceed the operating pressure of the media packed in the column.

NOTICE Before moving the adapter, make sure that the inside of the column tube is thoroughly lubricated with 20% ethanol.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 25

2 Safety instructions 2.1 Safety precautions

NOTICE Before beginning to move the adapter upwards, make sure that outlet tubing is not in contact with waste/drainage fluid. Otherwise when the adapter begins to rise the resultant suction effect may result in waste/drainage fluid being unintentionally drawn into the column.

Maintenance

WARNING Only properly trained personnel, or personnel with equivalent knowledge of similar equipment, are allowed to perform mainte- nance work on the column. Only personnel authorized by GE are allowed to perform service work on the AxiChrom column and AxiChrom Master.

WARNING Follow all of the user documentation safety instructions and the instructions provided by the AxiChrom Master when performing maintenance on the column.

WARNING Follow the instructions provided by the manufacturer and all addi- tional user documentation safety instructions when using the lifting device.

WARNING Make sure the lifting device is in perfect working order before use. If any damage is suspected, do not use the lifting device.

26 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.1 Safety precautions

WARNING When the lifting device is in use, make sure never to place any body part under the arms of the lifting device, or between column com- ponents and the lifting device.

WARNING Only spare parts and accessories that are approved or supplied by GE may be used for maintaining or servicing AxiChrom.

WARNING Make sure that all locking pins and lock blocks are mounted in their proper, marked positions before proceeding with the next step in the maintenance procedure, as described in the AxiChrom Master wizard instructions.

WARNING AxiChrom 1200-1600 columns. Make sure that all safety rods and lifting blocks are firmly in place before performing any maintenance or service work under the column.

WARNING Decontaminate before maintenance. To avoid personnel being exposed to potentially hazardous substances, make sure that the column is properly decontaminated and sanitized before mainte- nance or service.

WARNING Do not perform any type of maintenance work on the column while the column is pressurized. Note that the column can be pressurized even when the system is closed down.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 27

2 Safety instructions 2.1 Safety precautions

WARNING Do not perform any type of maintenance work on the column while the column contains chromatography media or liquids.

WARNING The bed support and distributor assemblies are heavy, particularly on larger columns. Make sure that these components are securely supported and cannot fall when fastening nuts or bolts are re- moved.

CAUTION Make sure that the piping system is completely leakage free before performing any Cleaning-In-Place (CIP) or Sanitation-In-Place (SIP) on the column.

CAUTION Take care not to trap fingers or other body parts when working with the bed support lifting device.

CAUTION Make sure that no body parts become trapped between the column tube assembly and the column frame when using the swing out function for maintenance work on the column. The column tube must be locked in place during all maintenance work. Due to the weight of the column tube assembly, it is not advisable to try to stop the momentum of the assembly by hand when it is set in motion.

CAUTION A sudden release of pressure when disconnecting tubing may cause injury.

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2 Safety instructions 2.1 Safety precautions

CAUTION The air from damaged pneumatic tubes may cause injuries. Replace any damaged tubes.

CAUTION Do not use force to fit lock blocks. Lock blocks that are difficult to screw into place may be damaged and must be replaced.

CAUTION The storage solution may evaporate over time when the column is stored. This may create an increase in pressure in the column. Make sure that all valves are closed, then remove the TC-clamp and blind cap from the bottom slurry inlet and carefully open the valve to release pressure from the column.

NOTICE Perform a leakage test using a harmless liquid, such as water, whenever any of the wetted parts have been re-fitted or changed.

NOTICE Always wear disposable gloves when handling the bed support to prevent grease from unprotected fingers coming into contact with the bed support.

NOTICE To avoid spillage, do not open the bottom valve while the column is filled with liquid, unless there are hoses connected to the outlets.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 29

2 Safety instructions 2.1 Safety precautions

NOTICE Handle stainless steel parts, especially the bed supports, with care. Damage to surfaces may lead to corrosion.

NOTICE Do not use metal implements that can scratch during maintenance and repairs, for example when removing O-rings.

NOTICE Make sure that cleaning of O-ring grooves is performed each time O-rings are removed and replaced. Clean O-ring grooves manually and gently with 20% ethanol, taking great care not to damage the sealing surface.

30 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.1 Safety precautions

2.2 Labels

Introduction This section describes the identification labels, safety labels and labels concerning haz- ardous substances that are attached to the AxiChrom columns and Master.

Column label

Example labelLabel placement

MeaningLabel text

Name of the productAxiChrom™

Dimensions of the columnDimension

Serial number for the individual columnSerial no.

Date/year when the column was manufacturedYear of manufacturing

Weight of the column when empty (kg)Tare mass

Operating temperature range (degrees Celsius)Operating temperature TS

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 31

2 Safety instructions 2.2 Labels

MeaningLabel text

Maximum column bed volumeMax column volume V

Design pressure limits for the column (bar g)Design pressure PS

Pressure that the column has been tested to tolerate (bar g and date of testing)

Test pressure PT

Maximum weight of the filled column (kg)Maximum filling mass

Pressure Equipment Directive fluid groupPED fluid group

Column information assigned by the customerCustomer information

Label text assigned by the customerLabel name

AxiChrom Master label The illustration below shows an example of an AxiChrom Master label.

MeaningLabel text

Product code number for AxiChrom MasterCode Number

Serial number for the individual AxiChrom MasterSerial Number

Year when the AxiChrom Master was manufac- tured

Manufacturing Year

Permissible supply voltage range (Europe)Supply Voltage Range (CE)

Maximum permissible supply voltage (North America)

Max Voltage (NA)

Supply voltage frequencyFrequency

32 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.2 Labels

MeaningLabel text

Maximum power consumptionMax Power Consumption

Enclosure rating according to UL 50EEnclosure

Highest current used by the motor (Full Load Ampere)

Largest Motor Current (FLA)

Highest current used by the AxiChrom MasterMax Current (FLA)

Required pressure for pressurized air supplyPneumatic Supply

Maximum tolerated short circuit currentShort Circuit Rating

Protection class rating according to EN 60529Protection Class

Conforms to ANSI/UL standard 61010-1. Certified to CAN/CSA Standard C22.2 Number 61010-1

Conforms to ANSI/UL Std. 61010- 1 Cert. to CAN/CSA Std. C22.2 No. 61010-1

Manufacturing site informationMfn Site : AQ1

Code number for field wiring diagramField Wiring Diagram 29048507

Symbols used in safety labels

MeaningLabel

Warning! Read the user documentation before using the system. Do not open any covers or replace parts unless specifically stated in the user documentation.

The system complies with applicable requirements for Australia and New Zealand.

This symbol indicates that the system has been certified by a Nationally Recognized Testing Laboratory (NRTL). An NRTL is an organization that the Occupational Safety and Health Administration (OSHA) has recognized as meeting the legal requirements in USA title 29 of the Code of Federal Regulations (29 CFR) Part 1910.7.

The system complies with applicable European directives.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 33

2 Safety instructions 2.2 Labels

MeaningLabel

This symbol indicates risk of crush injury.

Labels concerning hazardous substances

MeaningLabel

This symbol indicates that waste electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected sep- arately. Please contact an authorized representative of the manufacturer for information concerning decommissioning of equipment.

This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard GB/T 26572 Re- quirements for Concentration Limits for Certain Hazardous Substances in Electronics.

34 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.2 Labels

2.3 Emergency procedures

Introduction This section describes how to shut down the AxiChrom Master in an emergency. The section also describes the result in the event of power failure.

Emergency shutdown of AxiChrom Master

In an emergency situation, press the emergency stop button on the AxiChrom Master to stop adapter movement.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 35

2 Safety instructions 2.3 Emergency procedures

WARNING The EMERGENCY STOP button will not shut off the electrical power to the motor of the column. Use the Power Switch on the AxiChrom Master connector panel to shut off the electrical power.

WARNING The column may still be pressurized when the process has been stopped using the EMERGENCY STOP button. Make sure that the pressure is released before disconnecting any tubing.

Emergency shutdown of a connected pumping system

In an emergency situation when the pumps of a connected system, such as an ÄKTAprocess system have to be stopped, follow the emergency procedures described in the safety instructions provided by the manufacturer of the system. If the connected system is an ÄKTAprocess system the pumps are stopped by pressing either of the emergency stop buttons on the system.

36 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.3 Emergency procedures

Power failure

will result in...Power failure to...

Information about the ongoing procedure is lost. However, during maintenance mode the last static position is remem- bered so that the maintenance procedure can be resumed.

AxiChrom Master

The pumps stop and the valves revert to the default position (closed).

ÄKTAprocess

For further information, refer to ÄKTAprocess user documenta- tion or contact your local GE representative.

NOTICE When power is restored it is very important that the correct position of the adapter is verified, otherwise column damage may result.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 37

2 Safety instructions 2.3 Emergency procedures

2.4 Safety zones and access areas

Introduction Unless it is necessary for an operator to be close to the column to perform a specific procedure, personnel should remain a safety distance away from the column when any part of the column is in motion, and especially during movement of the adapter or column tube.

This section describes areas around the column where operators, maintenance and service personnel must take special care to avoid the risk of injury.

Description of safety zones

1 m

A B

DescriptionZone

Safety zone (red). An area of one meter around the AxiChrom column that is off limits for personnel when column parts are in motion.

Service access area (blue). A larger area around the column that is used during column maintenance and service.

Connection safety zone (yellow). The area between the AxiChrom Master and the column that is off limits for personnel when cables and tubing connections are in use.

Approximate location (orange) of a connected system, such as an ÄKTApro- cess system (optional). Note that there will be additional off limit zones containing cables and tubing connections when a system is connected to the column.

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2 Safety instructions 2.4 Safety zones and access areas

Safety zone dimensions

1600140012001000800600450400300Column size, mm

40003800350033003100280027002600Safety zone A length, mm

440043004100380035003200Safety zone A width, mm

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 39

2 Safety instructions 2.4 Safety zones and access areas

2.5 Crush injury risks

Introduction This section describes areas within safety zone A where particular care must be taken to avoid the risk of crush injury to fingers or hands when different parts of AxiChrom columns are in motion.

Specific areas to avoid Keep fingers and other body parts away from the following areas when column parts are in motion.

Crush injury risk areaMovement

Adapter moving up

40 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.5 Crush injury risks

Crush injury risk areaMovement

Adapter moving up

Adapter moving down

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 41

2 Safety instructions 2.5 Crush injury risks

Crush injury risk areaMovement

Adapter moving down

Column tube moving down

42 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.5 Crush injury risks

Crush injury risk areaMovement

Column tube swinging in- wards

• Crush risk between column tube and bot- tom distribution as- sembly (shown in im- age)

• Crush risk between column tube and adapter (not shown)

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 43

2 Safety instructions 2.5 Crush injury risks

2.6 Recycling information

Decontamination The equipment shall be decontaminated before decommissioning and all local regulations shall be followed with regard to scrapping of the equipment.

Disposal, general instructions When taking the equipment out of service, different materials must be separated and recycled according to national and local environmental regulations.

Recycling of hazardous substances

AxiChrom columns contain hazardous substances. Detailed information is available from your GE representative.

Disposal of electrical components

Waste electrical and electronic equipment must not be disposed as unsort- ed municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning decommissioning of equipment.

44 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.6 Recycling information

2.7 Declaration of Hazardous Substances (DoHS)

根据 SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下有关污染控制方面的信息。 The following product pollution control information is provided according to SJ/T11364-2014 Marking for Restriction of Hazardous Substances caused by electrical and electronic products.

电子信息产品污染控制标志说明 Explanation of Pollution Control Label

该标志表明本产品含有超过中国标准 GB/T 26572 《电子电气产品中限用物质的限量要求》中限量的有害物质。标志中的数字为本产品的环保使用期，表明本产品在正常使用的条件下，有毒有害物质不会发生外泄或突变，用户使用本产品不会对环境造成严重污染或对其人身、财产造成严重损害的期限。单位为年。为保证所申明的环保使用期限，应按产品手册中所规定的环境条件和方法进行正常使用，并严格遵守产品维修手册中规定的定期维修和保养要求。产品中的消耗件和某些零部件可能有其单独的环保使用期限标志，并且其环保使用期限有可能比整个产品本身的环保使用期限短。应到期按产品维修程序更换那些消耗件和零部件，以保证所申明的整个产品的环保使用期限。本产品在使用寿命结束时不可作为普通生活垃圾处理，应被单独收集妥善处理。 This symbol indicates the product contains hazardous materials in excess of the limits established by the Chinese standard GB/T 26572 Requirements of concentration limits for certain restricted substances in electrical and electronic products. The number in the symbol is the Environment-friendly Use Period (EFUP), which indicates the period during which the hazardous substances contained in electrical and elec- tronic products will not leak or mutate under normal operating conditions so that the use of such electrical and electronic products will not result in any severe environmen- tal pollution, any bodily injury or damage to any assets. The unit of the period is “Year”.

In order to maintain the declared EFUP, the product shall be operated normally ac- cording to the instructions and environmental conditions as defined in the product manual, and periodic maintenance schedules specified in Product Maintenance Pro- cedures shall be followed strictly.

Consumables or certain parts may have their own label with an EFUP value less than the product. Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures.

This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 45

2 Safety instructions 2.7 Declaration of Hazardous Substances (DoHS)

有害物质的名称及含量 Name and Concentration of Hazardous Substances

产品中有害物质的名称及含量 Table of Hazardous Substances’ Name and Concentration

有毒有害物质或元素 Hazardous substance

部件名称 Component name

多溴二苯醚 PBDE

多溴联苯 PBB

六价铬 Cr6+

镉 Cd

汞 Hg

铅 Pb

00000X29-0465-43

00000X29-0105-22

00000X29-0105-94

00000X29-0105-91

00000X29-0106-01

00000X29-0105-90

00000X29-0105-99

00000X29-1147-97

00000X29-1147-98

00000X29-1147-99

0: 表示该有毒有害物质在该部件所有均质材料中的含量均在SJ/T11363-2006 标准规定的限量要求以下

X: 表示该有毒有害物质至少在该部件的某一均质材料中的含量超出SJ/T11363-2006 标准规定的限量要求

• 此表所列数据为发布时所能获得的最佳信息

0: Indicates that this toxic or hazardous substance contained in all of the homogeneous ma- terials for this part is below the limit requirement in SJ/T11363-2006.

X: Indicates that this toxic or hazardous substance contained in at least one of the homoge- neous materials used for this part is above the limit requirement in SJ/T11363-2006.

• Data listed in the table represents best information available at the time of publication.

46 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

2 Safety instructions 2.7 Declaration of Hazardous Substances (DoHS)

3 System description

About this chapter This chapter contains a general description of AxiChrom columns and the AxiChrom Master.

Part annotations in the descriptive illustrations and tables in this document are not intended to represent the part number designations usedwhenordering spare parts.

Note:

Refer to the appropriate column documentation, such as the Material Confor- mity and Spare Parts list when ordering replacement parts, see Section 12.7 Ordering information, on page 328.

In this chapter This chapter contains the following sections:

See pageSection

483.1 Column description

523.2 AxiChrom 300-1600 Master description

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 47

3 System description

3.1 Column description

Introduction AxiChrom columns are available in two tube lengths for each column diameter, for maximum bed heights of 300 and 500 mm respectively. Column tubes are available in transparent acrylic or stainless steel. AxiChrom columns are operated from an AxiChrom Master, either as a stand-alone unit or controlled in turn from an ÄKTAprocess instrument running UNICORN software.

Column inlet and outlet flow paths

The media valve at the bottom of AxiChrom columns and the rinse nozzle at the top of AxiChrom 1200-1600 columns each have two separate flow paths.

One flow path, the mobile phase flow path, is for the introduction of mobile phase into the column. Through this flow path liquid has to flow through the bed supports to reach the process chamber of the column.

The adapter inlet/outlet of AxiChrom 300-1000 columns has only the mobile phase flow path through the bed supports to the process chamber of the col- umn.

Note:

The second flow path has no connection to the mobile phase flow path. When this second path is open, direct introduction to, or evacuation from, the column chamber is possible. For example:

• The slurry inlet tube of the media valve at the bottom of the column can be connected to a slurry tank, from where the chromatography media slurry is drawn into the column.

• Air can be evacuated from the column via the rinse nozzle inlet of AxiChrom 1200- 1600 columns.

• The rinse nozzle inlet of AxiChrom 1200-1600 columns can be used to introduce fluid for rinsing after unpacking.

If the rinse nozzle inlet of AxiChrom 1200-1600 columns is used to introduce fluid for rinsing, the manual control mode must be used to move the adapter to the intended position.

Note:

The slurry inlet of the media valve at the bottom of the column and the rinse nozzle inlet at the top of AxiChrom 1200-1600 columns each have a contiguous liquid connection path to a rinse inlet. The rinse inlet in each case is used for cleaning and flushing the valves and tubing connections after filling and unpacking the column.

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3 System description 3.1 Column description

NOTICE Use of the AxiChrom 1200-1600 column rinse nozzle inlet during unpacking sequences is currently not supported.

Rinse nozzle overview The rinse nozzle of AxiChrom 1200-1600 columns is controlled by a pneumatic control switch that is located on the left hand side of the top panel of the AxiChrom 300-1600 Master.

The rinse nozzle has two positions, Closed and Open. The switch default position is Closed and the switch must be manually turned and held in the Open position to open the nozzle.

When the rinse nozzle is in the Closed position the flow path to the column chamber via the top rinse and nozzle inlet is closed. The only connection path into the process chamber at the top of the column is then through the top mobile phase inlet/outlet.

When in theOpen position, the tip of the rinse nozzle projects into the inside the column. This enables:

• use of the rinse nozzle to evacuate any air bubble in the center of the column during the final stages of filling the column during priming,

• use of the rinse nozzle to introduce fluid to rinse out any residual media in the column after unpacking, with the adapter in manual control mode.

NOTICE The rinse nozzle must never be in the Open position when the col- umn tube is swung out or swung in during column maintenance. Make sure also that the rinse nozzle control switch on the top panel of the AxiChrom 300-1600 Master is never turned to theOpen position when there is a distance of less than 5 cm between the adapter and the bottom bed support. In the lowest adapter position the clearance distance is not sufficient for the rinse nozzle tip to extend.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 49

3 System description 3.1 Column description

Intelligent Packing Chromatography media is packed in the column using axial compression achieved by the column servo motor. Packing can be controlled using the appropriate AxiChrom Master wizard functions. Packing can also be performed and controlled using the UNICORN Intelligent Packing function of an ÄKTAprocess system for column sizes 300 to 1000.

ÄKTAprocess UNICORN Intelligent Packing functions are currently not com- patible for use with AxiChrom 1200-1600 columns as standard. Contact your local GE representative for more information about this.

Note:

Main components

4 5

1413127 2

50 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

3 System description 3.1 Column description

FunctionPartFunctionPart

Bottom bed support and distributor plate8Top unit1

Column stand9Worm gear and bellows2

Process chamber10Servo motor3

Column tube11300-100 columns: Top mobile phase in- let/outlet 1200-1600 columns: Rinse nozzle (includ- ing top mobile phase inlet/outlet)

Media valve slurry inlet12Adapter5

Media valve bottom mobile phase in- let/outlet

13Adapter bed support and distributor plate6

Media valve rinse inlet14Media valve assembly7

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 51

3 System description 3.1 Column description

3.2 AxiChrom 300-1600 Master description

Introduction The AxiChrom Master is a self-contained operator console for AxiChrom columns. The AxiChrom Master provides the user with interactive guides (wizards) for the performance of procedures such as priming, packing, unpacking and maintenance.

Use of the AxiChrom 300-1600 Master

The AxiChrom 300-1600 Master allows the operator to control the workflow, to use manual control to steer adapter movement, and to control air supply to the media valve (all column sizes) and rinse nozzle valve (1200-1600 columns only).

One AxiChrom 300-1600 Master unit can be used to control several columns (one at a time).

The user interface is a touch screen panel. Workflow and the opening and closing of the media valve are controlled via the user interface. The AxiChrom 300-1600 Master can also be used to control a slurry tank valve.

Pneumatic supply to the rinse nozzle valve is controlled by a switch located to the left of the touch screen panel. To open the rinse nozzle the operator turns the switch to the right and holds the switch in position for the required time.

Preferably use the sameAxiChrom300-1600Master to control a given column. The AxiChrom 300-1600 Master will remember the column configuration and the adapter position for the column.

Note:

52 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

3 System description 3.2 AxiChrom 300-1600 Master description

AxiChrom300-1600Mastermain components

FunctionPart

EMERGENCY STOP button1

User interface touch screen2

Rinse nozzle pneumatic control switch (for supply of air to the Rinse nozzle)3

Lockable wheels4

Electrical cabinet (at the rear of the AxiChrom 300-1600 Master)5

Pneumatic connections for supply of air to the media valve (black) and rinse nozzle (blue)1

Connection panel (at the rear of the AxiChrom 300-1600 Master)7

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 53

3 System description 3.2 AxiChrom 300-1600 Master description

FunctionPart

Maneuvering handle8

1 Rinse nozzle pneumatic connection is only applicable for AxiChrom 1200-1600 columns. The blue rinse nozzle air outlet tubing can be disconnected from the rear panel of the AxiChrom 300-1600 Master when using 300-1000 columns.

More information For information about the connection panel on the rear of the AxiChrom 300-1600 Master, see Connections on the AxiChrom 300-1600 Master, on page 68.

For information about power requirements, see Power requirements and connections, on page 76.

For information about starting the AxiChrom 300-1600 Master, see Section 5.2 Starting up and shutting down the AxiChrom 300-1600 Master, on page 82.

For information about updating AxiChrom 300-1600 Master software, see Chapter 9 Master maintenance, on page 295.

For information about the AxiChrom 300-1600 Master weight and dimensions, see Master weights and dimensions, on page 318.

54 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

3 System description 3.2 AxiChrom 300-1600 Master description

4 Installation

About this chapter This chapter contains information about AxiChrom installation, site requirements and how to set up AxiChrom columns for use.

In this chapter This chapter contains the following sections:

See pageSection

564.1 Site requirements

584.2 Transport

624.3 Unpacking

634.4 Wheel kits

654.5 Leveling the column

684.6 Connections

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 55

4 Installation

4.1 Site requirements

Site environment The following table describes the environmental requirements for the installation of the AxiChrom 300-1600 Master.

ValueAxiChrom 300-1600 Master requirements

380-480 V AC, 50-60 Hz with protective ground

Supply voltage

480 Y/277 V ACMax voltage (North America)

8500 VAMax power consumption

± 10% of nominal voltageMax mains supply voltage fluctuation

5.5 to 7 bar gPneumatic supply

+2°C to +30°CAmbient temperature, operating environment

+4°C to +30°CAmbient temperature, storage environment

Maximum relative humidity 80%Relative humidity

2000 mMaximum altitude1

2Pollution degree

1 According to the corresponding electrical standards fulfilled by the product.

The following table describes the environmental requirements for the installation of AxiChrom columns.

ValueColumn requirements

• for AxiChrom 300 - 1000 columns: 2°C to 30°C

• for AxiChrom 1200 - 1600 columns: 15°C to 30°C

Ambient temperature, operating environment

+4°C to +30°C1Ambient temperature, storage environment

Place the column on a level floor that can support the weight of the column

Placement

56 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

4 Installation 4.1 Site requirements

ValueColumn requirements

0% to 95%, non-condensingHumidity

1 It is recommended that the temperature of the storage environment does not exceed +24°C if the storage solution used in the column is 2% benzyl alcohol or 20% ethanol.

For additional information please refer to Section 12.1 Specifications, on page 312 and Site Preparation Guide.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 57

4 Installation 4.1 Site requirements

4.2 Transport

Introduction This section explains how to transport the crates and columns respectively, for the various column sizes:

• Transport in crate: 300-1000 columns, on page 58

• Moving 300-1000 columns, on page 59

• Transport in crate: 1200-1600 columns, on page 60

• Moving 1200-1600 columns, on page 61

For information about unpacking the column, see Section 4.3 Unpacking, on page 62. Unpacking instructions are also attached to the outside of the crate on delivery. For a list of the documentation provided with each column size, see AxiChrom instruction manuals, on page 13.

Note:

Transport in crate: 300-1000 columns

WARNING Due to the significant weight of AxiChrom columns, suitable equipment with adequate lifting capacity must be used when moving the unit or performing maintenance procedures.

• Make sure that all lifting equipment has an adequate capacity to safely lift the weight of the crate and column.

• Make sure that equipment used to move crates or columns over a sloping floor has adequate braking power to control the momentum and prevent the crate or column from moving too fast.

For information on crate weights and dimensions, see Crate weights and dimensions, on page 318.

The illustration below shows how the crate should be moved. Carefully read and follow the labels and symbols on the crate.

58 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

4 Installation 4.2 Transport

Symbols: - This side up - Fragile (glass) - Do not expose to water

Center of gravity

Arrows show where to place the lifting arms of the forklift/ pallet lifter

Moving 300-1000 columns

WARNING Tipping risk.When lifting AxiChrom crates or columns using a lifting truck, make sure that the crate or column is positioned so that there is no risk of the lifting equipment tipping over.

WARNING

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 59

4 Installation 4.2 Transport

For information on column weights and dimensions, seeColumndimensions, on page314 and Column weights, on page 315.

The illustration below shows how the column should be moved. Carefully read and follow the labels and symbols on the column. For information on wheel kits, see Section 4.4 Wheel kits, on page 63.

A B C

D E F

FunctionPart

The center of gravity should be centered over the forks (red in illustration) of the lifting equipment.

A + B

Shows where not to lift.C

The labels indicate how to place the forks. D: Centralized on sizes 450 to 1000. F: Shifted to the left on sizes 300 and 400.

D + F

The label indicates the center of gravity.E

Transport in crate: 1200-1600 columns

For information on how to transport the crates, refer to theAxiChrom1200-1600 columns Unpacking Instructions.

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4 Installation 4.2 Transport

Moving 1200-1600 columns After unpacking from the transport crate, AxiChrom 1200-1600 columns can be moved to the intended process position by lifting using eyebolts and an overhead crane or hoist. Alternatively AxiChrom 1200-1600 columns can be rolled over an even surface using a wheel kit that is available as an optional accessory for the column, see Wheel kits for AxiChrom 1200-1600 columns, on page 63, or the columns can be moved using other suitable capacity moving equipment, such as air castors.

NOTICE Do not place any type of lifting or moving equipment under the column feet.

Please note that the columns are heavy and cannot be easilymoved. Carefully read the Site Preparation Guide and Unpacking Instructions before placing the column.

Note:

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 61

4 Installation 4.2 Transport

4.3 Unpacking

Read before unpacking Instructions for unpacking AxiChrom columns and the AxiChrom 300-1600 Master are attached to the outside of the crate on delivery. The instructions for unpacking AxiChrom columns vary, depending on the column size, see AxiChrom instruction manuals, on page 13 for more information.

The instructions in the unpacking documents provide information that is important for safety during the unpacking of AxiChrom columns. Read and follow the instructions carefully when unpacking the crates.

62 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

4 Installation 4.3 Unpacking

4.4 Wheel kits

Introduction Wheel kit options are available for moving AxiChrom columns of different sizes.

AxiChrom wheel kits are intended for use with commercially available suitably dimen- sioned moving equipment, such as various models of MasterMover™ or a PowerAttack™, but the wheel kits do not include the equipment necessary for moving the AxiChrom columns after fitting the wheels to the column.

The type and model of moving equipment required for use with an AxiChrom wheel kit differs according to column size. For further advice about suitable types and models of moving equipment for specific column sizes, contact your local GE representative.

Detailed instructions explaining how to use wheel kits to move AxiChrom columns are provided with the wheel kit.

Wheel kits for AxiChrom300-600 columns

The wheels for 300-600 columns are delivered in a kit containing rear wheel units, a lever, a trolley bar and a trolley.

The wheel kits for 300-600 columns are intended for use with the trolley bar that is pro- vided as part of the wheel kit.

Wheel kits for AxiChrom800and 1000 columns

Wheels for 800 and 1000 columns are delivered in a kit containing a jack, a trolley bar, brackets and tools.

All of the equipment needed for fitting and removing the wheels and trolley bar is stored in the box on the trolley.

Wheel kits for AxiChrom 1200-1600 columns

Wheels for 1200, 1400 and 1600 columns are delivered in the form of wheel trolley kits with separately ordered accessory components according to column size.

AxiChrom 1200-1600 wheel kits contain a jack, a front trolley unit, two back trolley units, spacer blocks with protective rubber surfaces for the front and back trolley units.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 63

4 Installation 4.4 Wheel kits

The wheel kits for AxiChrom 1200-1600 columns must be supplemented with the correct moving arm for the column size. A suitable adapter attachment is also needed to fasten the column to the commercially available moving equipment that is to be used with the wheel kit.

A hitch attachment adapter to fasten AxiChrom 1200-1600 columns to a PowerAttack can be ordered as an accessory from GE. Adapters for attaching AxiChrom columns to a MasterMover are ordered via the manufacturer. For further details refer to the AxiChrom product pages on the GE website.

Note:

64 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

4 Installation 4.4 Wheel kits

4.5 Leveling the column

Introduction After the column has been moved to its process location the column must be leveled before use.

The column must be leveled before use so that the angle of discrepancy from the horizontal across the diameter of the columndoes not exceed 0.2 degrees.

Note:

Procedure

NOTICE Relieve the pressure on the column feet by raising the column using a jack or similar lifting and supporting equipment while adjusting the feet. Screw threads may be damaged if uneven pressure is placed on the column feet.

NOTICE Make sure that the column is level after it has been moved. If the column is not level, column performance may be negatively affect- ed.

ActionStep

Place jacks or similar suitable lifting equipment under the column stand, and use these to relieve the pressure on the column feet while making lev- eling adjustments.

Check that there is even load on all 4 feet.2

Loosen the locking nuts on the column feet.3

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 65

4 Installation 4.5 Leveling the column

ActionStep

Place a spirit level diagonally across the top of the column flange. Adjust the level of the column by screwing the column feet towards or away from the column stand as required until the column stands level.

NOTICE Do not adjust the feet out from the column more than 160 mm (AxiChrom 300-1000)/ 220 mm (AxiChrom 1200-1600) from the column base to the floor.

Move the spirit level to different places around the column flange, changing the angle measured by 90 degrees each time, as shown in the image, and make sure that the column is level in all directions.

When the column feet have been adjusted and the column is level, finger- tighten the locking nuts on the feet and then tighten the nuts using a wrench.

To make sure that the column is level before beginning priming or packing procedures, the column can be filledwithwater and thewater level measured around the circumference of the column tube. If the distance between the surface of the water and a fixed point on the column (such as the bottom flange, top flange or the top of the column tube) deviates sufficiently to indicate that the angle of discrepancy from the horizontal across the diameter of the column exceeds 0.2 degrees then the column leveling must be adjusted.

Tip:

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4 Installation 4.5 Leveling the column

The deviation in the distance between the surface of the water and a fixed point on the column inmillimeters that corresponds to a discrepancy in excess of 0.2 degrees will depend on the diameter of the column. The maximum limit value can be calculated by multiplying the column diameter by 0.0035, for example:

• for a 400 mm diameter column, 400 × 0.0035 = 1.4 mm

• for a 1000 mm diameter column, 1000 × 0.0035 = 3.5 mm

• for a 1600 mm diameter column, 1600 × 0.0035 = 5.6 mm

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4 Installation 4.5 Leveling the column

4.6 Connections

Connections on the AxiChrom 300-1600 Master

The illustration below shows the AxiChrom 300-1600 Master connector panel.

1 32 4 5

610 9 8 7

DescriptionPartDescriptionPart

Pressure sensor connector7Pneumatic air supply1

Motor feedback8Slurry tank control2

Power supply9Motor power cable3

Power switch10USB4

Rinse nozzle air outlet (blue tube)11Media valve air outlet (black tube)

PROFIBUS™ signal cable connec- tor

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4 Installation 4.6 Connections

Connections on the column The illustration below shows the connection points on the column.

17161514

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4 Installation 4.6 Connections

DescriptionPart

Motor power and motor feedback communication cables, with cable clamp

Note:

The purpose of the clamp on the motor power and motor feedback connection cables is to make sure that both of the cables are con- nected and disconnected at the same time.

For 300-1000 columns: Top mobile phase inlet/outlet13

For 1200-1600 columns: Rinse nozzle (including top mobile phase inlet/outlet)

Slurry inlet/outlet14

Bottom mobile phase inlet/outlet15

Media valve air inlets (2 inlets)16

Rinse inlet17

Pressure sensor (mounted on hose to a system)18

For 1200-1600 columns only: Rinse nozzle air inlets (2 inlets)119

1 The insert image shows the rinse nozzle air inlets located to the side of three tube rollers. Note that the number of tube rollers may differ according to column size and configuration.

How to connect the column to the AxiChrom 300-1600 Master and an external system

The table below shows how to connect the column to the Master and an external system. Numbers in parentheses refer to illustrations of the:

• Master in Section Connections on the AxiChrom 300-1600 Master, on page 68.

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4 Installation 4.6 Connections

• Column in Section Connections on the column, on page 69.

When rupture discs are used, make sure that these are positioned so that venting after rupture is directed to waste and not towards an area where personnel could be endangered.

ToFrom

Wall socket air outlet (5.5-7 bar)Pneumatic air supply (1) on the Master

Motor power on the column (12)1Motor power cable (3) on the Master

Motor feedback (12) on the columnMotor feedback (8) on the Master

Pressure sensor mounted on column (18)Pressure sensor connector (7) on the Master

PROFIBUS connection on ÄKTAprocessPROFIBUS signal cable (6) on the Mas- ter

Media valve air inlets (2 inlets) (16) on the columnMedia valve air outlets (2 outlets) (5) on the Master (black tube)

Slurry tankSlurry inlet (14) on the column

Bottommobile phase on a system (Column1 bottom valve on ÄKTAprocess) 2

Bottom mobile phase (15) on the col- umn

Ground (See Grounding the column, on page 78)

Protectiongroundcableon the column stand

Power supply connector (380-480 VAC, 50-60 Hz) with pro- tective ground

Power supply cable (9) on the Master

A system (for AxiChrom 300-1000 columns, a CIP2 Inlet on ÄKTAprocess)

Media valve rinse inlet (17) on the col- umn

Mobile phase on a system (CIP1 Inlet on ÄKTAprocess)2Top mobile phase (13) on the column

AxiChrom 1200-1600 columns:

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4 Installation 4.6 Connections

ToFrom

To an external pump3Rinse Nozzle inlet (13) on the column

Rinse nozzle air inlets (19) (2 inlets). Pneumatic connection points located to the side of the tube roller unit.

Rinse nozzle air outlets (2 outlets) (11) on the Master (blue tube)

Pneumatic connections on the Rinse nozzle (2 inlets), for de- tails see Rinse nozzle parts, on page 208.

Rinse nozzle air inlets (2 inlets) (19)

BioProcess Slurry Tank automatic slurry tank valve (tank and valve options available from GE)

Slurry tank control (2) on the Master

1 The power cable is connected to the column together with the motor feedback (encoder) connection cable and cable clamp. It is important that these connectors are fully tightened. The illustration shows an incorrectly tightened connection (A) and a correctly tightened connection (B).

A B

2 Use pressure safety equipment on the mobile phase, such as a rupture disc, to make sure that the maximum operating pressure of the column is not exceeded.

3 For pump flow capacity recommendations for individual column sizes and applications, contact your local GE representative.

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4 Installation 4.6 Connections

Recommended mobile phase tubing inner diameters

Note: • All top and bottommobile phase tubing dimensions are given inmillimeters (mm), while the system dimensions are stated in millimeters or inches.

The following tables provide general guidelines for tubing inner diameters for top and bottom mobile phase connections when connecting AxiChrom 300-1600 columns to ÄKTAprocess or to other process systems that have different dimensions.

Use narrower tubing for packing gel filtration or polishing media.Tip: Use the widest tubing compatible with the system for high flow media such as Capto™ S.

Connecting AxiChrom 300-1000 columns to ÄKTAprocess for Intelligent Packing

A dash (-) in the table means that the combination is not compatible with ÄKTAprocess UNICORN Intelligent Packing functions.

Note:

PP represents polypropylene and SS represents stainless steel.

1000800600450400300 ÄKTAprocess dimension

--6.4 or 9.46 mm PP

--6.4 or 9.43/8" SS

-9.4, 12.7, or 19.16.4, 9.4, or 12.710 mm PP

-9.4, 12.7, or 19.16.4, 9.4, or 12.71/2" SS

25.4 or 38.19.4, 12.7, or 19.1-1" PP or SS

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4 Installation 4.6 Connections

ConnectingAxiChrom1200-1600 columns to ÄKTAprocess or equivalent process system

Note:

PP represents polypropylene and SS represents stainless steel.

160014001200 ÄKTAprocess or equivalent process system dimension

Not applicable25.4 or 38.11" PP or SS

25.4, 38.1 or 47.51½" PP or SS

38.1 or 47.5Not applicable2" PP or SS

Rupture discs Pressure safety equipment must be connected to the column mobile phases to ensure that the maximum operating pressure of the column is not exceeded.

Pressure safety equipment in the form of rupture discs suitable for use with AxiChrom columns are available from GE.

Installation of rupture discs will reduce the maximum operating pressure for the column, as rupture discs are designed to burst to prevent the maximum pressure from being reached. The rupture discs that are available from GE for use with AxiChrom columns will reduce the maximum operating pressure for the column from 4 bar g to 3.8 bar g.

Note:

Special T-junctions for mounting rupture discs are provided for use with AxiChrom 300- 600 column sizes. Use these junction only with rupture discs recommended by GE.

For AxiChrom 800-1000 column sizes 1" T-junctions (part no. 28938170) are available.

For AxiChrom 1200-1600 column sizes 1.5" T-junctions (part no. 28938171) are available.

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4 Installation 4.6 Connections

Positioning the bypass valve The bypass valve block (currently an option available for AxiChrom 1200-1600 columns) is mounted to the front fixed upright of the column stand using a mounting bracket. The mounting bracket is clamped to the front upright of the column stand by a U-rod.

When positioning the bypass valve it is important to make sure that there is sufficient space between the valve block and the column tube to allow unrestricted movement during maintenance.

The following illustration shows a correctly positioned bypass valve (A) and an incorrectly positioned valve (B).

A B

The height at which that the bypass valve should be positioned on the front fixed upright of the column stand will depend on the size of the column and the arrangement of the connecting tubing.

Note:

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4 Installation 4.6 Connections

Power requirements and connections

The general power requirements for the AxiChrom 300-1600 Master are:

ValueRequirement

380-480 V AC 3~Supply voltage range

480 Y/277 V ACMax voltage (North America)

14.5 ALargest motor current (Full Load Ampere)

21.1 AMaximum current (Full Load Ampere)

50 - 60 HzFrequency

8500 VAMax power consumption

5 kAShort circuit rating

Detailed requirements may vary for different column configurations. Refer to the column documentation for the applicable requirements for your specific column and AxiChrom Master.

Note:

The power cables for both the AxiChrom 300-1600 Master and for the adapter control motor are delivered attached to the AxiChrom 300-1600 Master.

The system shall be connected to a 400 V supply voltage system or a 480 V supply voltage system with a maximum voltage of 277 V between phase and ground. A suitable con- nector has to be selected and assembled on the AxiChrom 300-1600 Master by the customer to comply with the supply voltage system and local regulations.

The Motor Power cable for the adapter control motor is permanently installed on the AxiChrom 300-1600 Master. The motor feedback communication cable is connected to Motor Feedback. Both these cables must be connected to the motor before the AxiChrom 300-1600 Master is connected to the power outlet. The cable clamp must also be installed, see Connections on the column, on page 69.

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4 Installation 4.6 Connections

Color codingof cable conductors The power cable connections are color coded as shown in the following table.

ColorConductor

Green/yellowProtective ground (earth)

No 1 or BlackLive 1

No 2 or BrownLive 2

No 3 or GreyLive 3

The black, brown and grey conductors may be connected to any of Live 1, 2 and 3. The phase connection is detected automatically.

Precautions

WARNING All electrical installations must be performed by authorized person- nel only.

WARNING Only properly trained personnel who have read and understood theOperating Instructionsare allowed to open the AxiChrom Master cabinet.

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4 Installation 4.6 Connections

WARNING Always connect the AxiChrom Master to a power supply with pro- tective grounding.

WARNING Connection to the wrong power supply voltage may cause injury to personnel and damage to the equipment.

WARNING Power cables and connectors must be replaced or repaired by properly trained personnel, authorized by GE.

Grounding the column A grounding connection point is provided on the inside of the column stand of AxiChrom 300-1000 columns, to allow the column to be connected to the plant grounding network.

The following illustration shows the location of the grounding cable connection point on the column frame of AxiChrom 300-1000 columns.

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4 Installation 4.6 Connections

Compressed air requirements It is important for personal safety and safe operation to use the correct pressure and quality of compressed air for the pneumatic valve control. The basic requirements are:

• Free of oil and particles

• -30ºC dew point

• 5.5 to 7 bar

Pressure specifications in these Operating Instructions are given in bar. The pressure gauge is calibrated so that bar and bar g are equivalent.

Note:

The pneumatic air supply connections on AxiChrom Master are illustrated in Connections on the AxiChrom 300-1600 Master, on page 68.

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4 Installation 4.6 Connections

5 Operation

About this chapter This chapter provides general instructions on how to prepare and operate AxiChrom columns.

In this chapter This chapter contains the following sections:

See pageSection

815.1 AxiChrom 300-1600 Master and UNICORNsoftware

825.2 Starting up and shutting down the AxiChrom 300-1600 Master

885.3 Cleaning a new column

905.4 Performing a leakage test

955.5 Preparing slurry and buffer

965.6 Priming the column

1025.7 Packing the column

1045.8 Cleaning a packed column

1055.9 Unpacking the column

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5 Operation

5.1 AxiChrom 300-1600 Master and UNICORNsoftware

AxiChrom 300-1000 columns AxiChrom 300-1000 columns are operated via an AxiChrom 300-1600 Master or an AxiChrom 300-1000 Master, either as a stand-alone unit or controlled in turn from an ÄKTAprocess system running UNICORN software. There are no manual controls for AxiChrom 300-1000 columns.

AxiChrom 1200-1600 columns AxiChrom 1200-1600 columns are operated as a stand-alone unit using the AxiChrom 300-1600 Master. The manual Rinse nozzle pneumatic switch on the top panel of the AxiChrom 300-1600 Master is used to operate the rinse nozzle.

Note:

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 81

5 Operation 5.1 AxiChrom 300-1600 Master and UNICORNsoftware

5.2 Starting up and shutting down the AxiChrom 300-1600 Master

Introduction This section describes how to start the AxiChrom 300-1600 Master and login to access the AxiChrom Master main menu functions.

How to correctly shut down the AxiChrom 300-1600 Master is also described.

User access levels There are different user access levels for the AxiChrom Master software. While the User access level is sufficient to select a column and perform priming and packing operations, Superuser access is needed, for example, for the operator to be able to create new columns on the column list.

The different user level access functions are shown in the following table.

User levelButton/functionScreen

MaintenanceSuperuserUser

×××LoginWelcome/Login

×××Select columnSelect column

××Add/Edit/Delete column

×××Verify adapter positionVerify adapter position

×××PrimingMain menu

××Intelligent packing

××Unpacking

×××Manual control

××Maintenance

×××System infoSelect column configuration

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5 Operation 5.2 Starting up and shutting down the AxiChrom 300-1600 Master

Login procedure Follow the instructions below to login to the AxiChrom 300-1600 Master software to access the wizard functions via the main menu screen.

ActionStep

Make sure that the AxiChrom 300-1600 Master is connected to the motor of the AxiChrom column as described in Connections on the column, on page 69.

Turn the Power Switch on the Master control panel to position I .2

Result: The Master starts and automatically runs a start-up program.

Click the Login button on the Welcome dialog screen.3

Result:A screen appears that allows users to login to the AxiChrom 300-1600 Master software with the appropriate level of user access.

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5 Operation 5.2 Starting up and shutting down the AxiChrom 300-1600 Master

ActionStep

Click the User and Password buttons, and enter the required information using the keyboard displayed on the screen. Click Enter to confirm the en- tered selections.

Result: Successful login will activate the Continue button on the Welcome screen.

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5 Operation 5.2 Starting up and shutting down the AxiChrom 300-1600 Master

ActionStep

ClickContinue to access the Select column screen. The AxiChrom 300-1600 Master will automatically detect the correct column from the list. Click Con- tinue.

Note:

The first time a columnmotor is connected to the AxiChrom300-1600Master, or following an AxiChrom300-1600Master firmware upgrade, the configura- tion for the connected column must be entered.

Verify that the data displayed for the column motor matches the column you are about to use, and then click Yes.

Result: Auto-calibration of the column motor will initiate.

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5 Operation 5.2 Starting up and shutting down the AxiChrom 300-1600 Master

ActionStep

Wait until the AxiChrom Master software has completed the auto-calibration of the column motor, and after calibration is successfully completed click Continue.

Result: The Verify adapter position screen will appear.

Verify that the adapter position displayed corresponds to the position mea- sured on the scale on the column. If the adapter position does not correspond to the position measured on the column scale, click Set to enter the correct adapter position.

NOTICE It is very important that the correct position of the adapter is entered, otherwise column damage may result.

Note:

For an illustration of where the column scale is located on the column, see Section 6.3 Power failure during maintenance, on page 113.

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5 Operation 5.2 Starting up and shutting down the AxiChrom 300-1600 Master

ActionStep

Click Continue to access the Main menu screen.9

Result: The wizard functions that are accessible to the user will be displayed in accordance with the access rights granted by the user level login.

Further information about the use of AxiChrom 300-1600 Master wizard functions are provided in the maintenance chapters of these Operating Instructions and in the AxiChrom User Manual.

Powering off To shut down the AxiChrom 300-1600 Master:

ActionStep

In the Main menu of the AxiChrom 300-1600 Master, press Power Off.1

When the text It is now safe to power off is displayed, use the Power Switch on the AxiChrom 300-1600 Master connector panel to turn off the power.

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5 Operation 5.2 Starting up and shutting down the AxiChrom 300-1600 Master

5.3 Cleaning a new column The column is delivered partially filled with a denatured 20% (v/v) alcohol storage solution. The column must be unpacked from the crate and cleaned as soon as possible after delivery, preferably within the first two to three months. Make sure that the column is free from damage before beginning to prepare it for use.

• Protective glasses

• Working gloves to protect against sharp edges

• Protective footwear, preferably with steel toe-cap

• Disposable gloves

Always use clean disposable gloves when manually handling parts.

Then...If...

Follow the instructions in Section 10.1 Cleaning an empty column, on page 301

The column is to be used soon

Unpack the column from the crate, check it and clean it according to the instructions below

Note:

If the column is not cleaned, there is a substantial risk of mold and corrosion attacks.

The column is to be used at a later time (within 3 months from delivery)

Cleaning procedure

Remove the TC-clamp and blind cap from the media valve mobile phase inlet/out- let.

Note:

Note that the column may be under slight pressure.

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5 Operation 5.3 Cleaning a new column

Connect a pump to the media valve mobile phase inlet/outlet.2

• Make sure that the tube from the pump is primed and that it is completely filled with liquid before it is connected to the media valve mobile phase in- let/outlet.

• The pump must be switched off during installation, so that the column does not become pressurized.

Remove the TC-clamp and blind cap from the top mobile phase tube and connect a tube that leads to waste.

Flush the column with at least 6 column volumes (calculated from an adapter position of 1 to 2 cm at delivery) of 20% ethanol.

Prepare the column for storage according to the instructions in Section 10.3 Storage, on page 304.

NOTICE If the column is filled and should be moved, plug the top mobile phase and media valve mobile phase inlets with TC-clamps and blind caps, diaphragms or other suitable valves.

The tube installed on the top mobile phase at delivery is only intended for storage solution conditioning and should be discarded once the column is in- stalled. The maximum operating pressure of the tube is 1 bar.

Note:

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5 Operation 5.3 Cleaning a new column

5.4 Performing a leakage test

Introduction Perform a leakage test when:

• the column is new,

• the column has been moved or transported,

• the O-rings have been replaced,

• other parts that may cause leakage have been replaced, or

• maintenance or service of the column has been performed.

Read the whole procedure before starting the test.Note:

When rupture discs are used, make sure that these are positioned so that venting after rupture is directed to waste and not towards an area where personnel could be endangered.

NOTICE Only liquid should be used in the column during the leakage test. Do not perform a leakage test on a column containing media.

Materials The equipment listed below is needed when performing a leakage test.

• Reference pressure gauge (calibrated, range: 0-10 bar)

• External pump with capacity up to 400 cm/h and 4 bar

• Three valves (and a Check valve if not included in the pump outlet)

• Pressure relief valve/rupture disc

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5 Operation 5.4 Performing a leakage test

• Water tank

• Pipes and bows to connect the pump, water tank, valves and pressure gauge

• End caps

Procedure Follow these instructions to perform a leakage test:

ActionStep

Make sure that water to be used for the leakage test is at the same temper- ature as the column before continuing.

Connect the Master to the column and start the Master.2

Open the bottom valve using the Master Manual control wizard.3

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5 Operation 5.4 Performing a leakage test

ActionStep

Set up the test with the AxiChrom column as illustrated below:4

Valve 1

Valve 2

Valve 3

Water tank

Pump

Waste

AxiChrom Pressure

gauge

Reference Pressure

gauge

1 Connect the water tank to the Pump inlet.

2 Connect Valve 1 to the Bottom mobile phase inlet/outlet on the AxiChrom column and seal the other two connections with end caps.

3 Connect a t-connector to:

• Valve 1

• the Pump outlet, and

• Valve 2 with a hose leading to waste

Note:

If necesssary, put a plastic block underneath the tubing to relieve any strain on the Bottom mobile phase inlet/outlet.

4 Connect the AxiChrom Pressure gauge for the Master, a Reference pressure gauge and shut-off Valve 3 to the Top mobile phase.

5 Connect a hose to Valve 3 that leads the water back to the tank.

6 Open Valve 3 fully.

Move the adapter so that the lower adapter scraper seal is positioned in the middle of the priming groove, creating a clear passage to the flushing channels, and so that the filling water can pass out through the flushing connection in later steps.

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5 Operation 5.4 Performing a leakage test

ActionStep

Fill the column gently by starting the pump at a low flow and opening Valve 1 completely (Valve 2 should be closed).

Continue filling the column with water and slow the filling rate when the water approaches the adapter. Allow water to flow out of the flushing con- nectors, and then stop the pump and move the adapter downwards, to below the priming groove.

Flush any remaining air from the column and hoses.

Note:

Use a pump flow that does not exceed 400 cm/h.

Stop the pump when all of the air has been removed.9

Move the adapter down to its maximum bed height (300 mm for a short column tube, or 500 mm for a long column tube) and make sure that all air is evacuated from the column.

Note:

The process chamber under the adaptermust be completely filledwith liquid.

Close shut-off Valve 3 completely.11

Use the pump to gently pressurize the column to the intended test pressure and allow the pressure to stabilize. The test pressure must not exceed the design pressure for the AxiChrom column (maximum 3.8 to 4 bar g).

Note:

It might take several minutes to adjust the pressure until it is stable.

NOTICE If rupture discs are used as the column pressure safety equipment, the test pressure must not exceed 3.8 bar g. Otherwise there is a risk that the disc will burst.

When a stable pressure has been achieved, close the shut-off Valve 1 and note the pressure and start time.

Monitor the pressure and let the column remain pressurized for 30 ±5 min- utes.

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5 Operation 5.4 Performing a leakage test

ActionStep

When the test period is completed, record the pressure and evaluate the result. The pressure drop should not exceed 0.20 bar during the test period (30 minutes).

If the pressure has dropped more and continues to drop when the 30 minutes have passed, there is a substantial risk that the column is leaking.

Note:

If no leak is found and there is a doubt about if the AxiChrom column is really leaking, redo the test before releasing the pressure. There might be a test valve that leaks.

Before releasing the pressure, check for liquid leakage, especially around the O-rings.

Release the pressure slowly, by slowly opening the Valve 1 and then slowly opening Valve 3.

Note:

If the pressure is released too fast, there is a slight risk that any remaining air will expand and damage the column when depressurized.

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5 Operation 5.4 Performing a leakage test

5.5 Preparing slurry and buffer

Before packing the column Before packing the column, perform the following:

• Prime the column

• Select an appropriate packing buffer

• Prepare slurry

• Determine the slurry concentration

More information about slurry preparation can be found in the AxiChrom User Manual.

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5 Operation 5.5 Preparing slurry and buffer

5.6 Priming the column

Introduction Before a process run is started the column and the system must be primed to remove air.

CAUTION When working with 20% v/v 1-propanol solutions:

• work in a well-ventilated area

• wear appropriate personal protective equipment

• keep storage containers tightly closed

CAUTION Before priming the column, make sure that tubing is connected to all rinse nozzle inlet/outlets and led to waste.

NOTICE Use particle-free water to prime the column. Do not use solutions containing ethanol or sodium hydroxide for column priming.

NOTICE Before moving the adapter, make sure that the inside of the column tube is thoroughly lubricated with 20% ethanol.

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5 Operation 5.6 Priming the column

Wetting plastic bed supports Plastic bed supports must be thoroughly wetted for optimal function. Follow one of the alternative instructions below if the column is fitted with plastic bed supports.

Before priming the column, mount the bed supports dry and assemble the column as described in Chapter 7 Maintenance of 300-1000 columns, on page 161 and Chapter 8 Maintenance of 1200-1600 columns, on page 198. Connect membrane valves on the column top and bottom mobile phases.

Note:

NOTICE To prevent pressure building in the empty column, make sure that both the top and bottom mobile phases are open when the adapter is moved.

Procedure: alternative 1

ActionStep

Prime the column according to the AxiChrom 300-1600 Master priming in- structions or a UNICORN priming method.

Perform a leakage test.2

With the adapter at 1 cm from the bottom bed support, equilibrate the col- umn with five column volumes of 20% v/v 1-propanol at 30 cm/h under a pressure of 1 bar.

Pause the flow. Use membrane valves to seal the column mobile phases and incubate for 2 hours at 1 bar.

Carefully release the pressure on the membrane valves.5

Flush the column with at least five column volumes of purified water or packing buffer.

Pack the column according to the AxiChrom 300-1600 Master or a UNICORN packing method.

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5 Operation 5.6 Priming the column

Procedure: alternative 2

NOTICE In this procedure priming and leakage testing is performed after the wetting procedure. If any sign of leakage is observed during wetting stop the procedure and do not continue until the leakage issue has been resolved.

ActionStep

Wet the scraper seals and spray the inside of the column with 20% v/v ethanol.

In the AxiChrom Master Main menu select Manual Control and lower the adapter at 20 cm/h until the adapter has passed the priming groove.

Fill the seal flush channel with 20% v/v ethanol after both the upper and lower scraper seals have passed the priming groove.

Move the adapter down at 100 to 300 cm/h until it reaches the 1 cm position.4

Fill the empty column with 20% v/v 1-propanol, up-flow via the mobile phase, and then recirculate the solution for 5 minutes at 30 cm/h.

Increase the flow to 90 cm/h and continue to recirculate the solution for an additional 5 minutes.

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5 Operation 5.6 Priming the column

ActionStep

Decrease the flow to 30 cm/h, and then switch the column valves to down- flow.

NOTICE Reduce flow before switching the flow direction of the column valves to avoid large pressure changes.

Increase the flow to 90 cm/h and continue to recirculate the solution for an additional 5 minutes.

Reduce the flow to 30 cm/h and recirculate five column volumes under a pressure of 1.5 bar g.

Pause the flow. Use membrane valves to seal the column mobile phases, and then allow the column to stand for 2 hours under a pressure of 1.5 bar g.

Carefully open the membrane valves to release the pressure, and then flush the column with at least five column volumes of purified water at 30 cm/h.

Prime the column according to the AxiChrom Master priming instructions, or using a UNICORN priming method.

Perform a leakage test.13

Using the Master wizard Before priming using the Master priming wizard, the media valve has to be primed manually.

Priming the media valve

ActionStep

Connect a pump to the rinse inlet.1

Connect the slurry hose/tank to the slurry inlet.2

Open the slurry hose/tank.3

Close the Bottom mobile phase inlet and the Media valve.4

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5 Operation 5.6 Priming the column

ActionStep

Start a flow through the rinse inlet and further into the slurry hose/tank, at approximately 100 cm/h.

Note:

Some AxiChrom columns have the same rinse inlet tubing dimensions, but the connected slurry hose dimension may vary. The flow rates stated below are general guidelines.

• 100 l/h for 300 columns

• 200 l/h for 400-600 columns

• 800 l/h for 800 and 1000 columns

• 1500 l/h for 1200, 1400 and 1600 columns

When the hoses are free from air, make sure the Top mobile phase inlet/outlet is open, and then open and close the Media valve from Manual Control in the Master a couple of times. To make sure no more air comes from the Media valve, continue to pump liquid through the valve, filling the bottom of the column and covering the bottom bed support with liquid to a depth of about 1 cm.

Note:

If the column is packed, do not open the media valve during priming.

When no more air comes up from the Media valve, close it.7

Stop the pump and close the slurry tank.8

Result: The Media valve has now been primed.

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5 Operation 5.6 Priming the column

Priming the column

ActionStep

Select the Priming wizard in the AxiChrom 300-1600 Master Main menu.1

Follow the instructions in the wizard to prime the column.2

Further information about Master wizards can be found in the AxiChrom User Manual.

Using a UNICORN method A priming method in UNICORN includes priming of both the media valve and the column.

ActionStep

In the UNICORN method wizard, choose to create a method for Priming.1

Follow the steps in the method wizard, make appropriate selections, and save the method.

Run the UNICORN method.3

Further information about how to create and run UNICORN methods can be found in the AxiChrom User Manual.

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5 Operation 5.6 Priming the column

5.7 Packing the column

Using the Master wizard

ActionStep

Select the Intelligent Packingwizard in theMainmenu in AxiChrom Master.1

Follow the instructions in the wizard to pack the column.2

Further information about Master wizards can be found in the AxiChrom User Manual.

Using the UNICORN method

ActionStep

In the UNICORN method wizard, choose to create a method for Column Packing, Packing Test, or the two combined.

Follow the steps in the method wizard, make appropriate selections, and save the method.

Run the UNICORN method.3

Further information about how to create and run UNICORN methods can be found in the AxiChrom User Manual.

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5 Operation 5.7 Packing the column

Performance evaluation of the column

See the AxiChrom User Manual for information about how to evaluate the performance of the packed column. An application note (28-9372-07) describing column efficiency testing is also available from GE.

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5 Operation 5.7 Packing the column

5.8 Cleaning a packed column

Cleaning-In-Place (CIP) Make sure that the media in the column withstands the chemicals used for CIP. For in- formation about which chemicals to use for CIP, refer to Section 12.4 Chemical resistance, on page 321.

CAUTION Make sure that the piping system is completely leakage free before performing any Cleaning-In-Place (CIP) or Sanitation-In-Place (SIP) on the column.

Sanitization-In-Place For recommendations on sanitization methods, refer to the AxiChrom User Manual.

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5 Operation 5.8 Cleaning a packed column

5.9 Unpacking the column

Using the Master wizard

ActionStep

Select the Unpacking wizard in the Main menu of the AxiChrom 300-1600 Master.

Follow the instructions in the wizard to unpack the column.2

Further information about AxiChrom wizards can be found in the maintenance instruction sections in this document and in the AxiChrom User Manuals.

Using the UNICORN method

ActionStep

In the UNICORN method wizard, choose to create a method for Column Unpacking.

Follow the steps in the method wizard, make appropriate selections, and save the method.

Run the UNICORN method.3

Further information about how to create and run UNICORN methods can be found in the AxiChrom User Manual.

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5 Operation 5.9 Unpacking the column

6 General maintenance and service

About this chapter Regular cleaning and maintenance of AxiChrom columns is essential for reliable results. It is important that the column is kept free from contamination such as microbiological growth and adsorbed proteins in the liquid handling system.

This chapter provides a general overview of the maintenance procedures and service schedules that apply to all AxiChrom column sizes.

For specific maintenance procedures that are performed in different ways depending on the column size, see respectively Chapter 7 Maintenance of 300-1000 columns, on page 161 and Chapter 8 Maintenance of 1200-1600 columns, on page 198.

In this chapter

See pageSection

1076.1 Service and preventive maintenance

1116.2 General maintenance tools

1136.3 Power failure during maintenance

1156.4 Putting the column in and out of maintenance mode

1426.5 Media valve maintenance

1556.6 Changing liquid in the flushing channels

1586.7 Changing flushing connector O-rings

1606.8 Leakage test

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6 General maintenance and service

6.1 Service and preventive maintenance

Introduction Regular service and maintenance of the column is necessary to maintain the column in optimal condition and extend the operational lifetime of column components.

Service and preventive maintenance work should be performed according to GE recom- mendations.

In this section:

See pageSection

1086.1.1 Service recommendations

1096.1.2 Cleaning before maintenance or service

1106.1.3 Maintenance schedules

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6 General maintenance and service 6.1 Service and preventive maintenance

6.1.1 Service recommendations

Service frequency The requirements for frequency of service and preventive maintenance depend on the frequency of use of the column and the specific applications performed.

A general recommendation is one service and preventive maintenance visit every two years, but if columns are in continuous operation an annual service and preventive maintenance visit is recommended.

Service agreements Contact your local GE representative for information about frequency of service require- ments to suit individual application needs and for details of the GE service agreement options available for AxiChrom columns.

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6 General maintenance and service 6.1 Service and preventive maintenance 6.1.1 Service recommendations

6.1.2 Cleaning before maintenance or service

Introduction Before any maintenance or service work can be performed the column must be properly emptied, cleaned and rinsed to remove any infectious or aggressive fluids. For instructions about how to clean the column before maintenance or service work is performed, see Chapter 10 Cleaning and storage, on page 300.

To ensure the protection and safety of service personnel, all equipment and work areas must be clean and free of any hazardous contaminants before a service engineer starts maintenance work.

Health and safety checklist The checklist in the On Site Service Health & Safety Declaration Form or the Health & Safety Declaration Form for Product Return or Servicing, must be completed depending on whether the instrument is going to be serviced on site or returned for service, respec- tively.

Copy the form you need from Section12.6Health andSafetyDeclaration Form, onpage326 or print it from the PDF file available on the User Documentation CD.

CAUTION Make sure that the piping system is completely leakage free before performing any Cleaning-In-Place (CIP) or Sanitation-In-Place (SIP) on the column.

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6 General maintenance and service 6.1 Service and preventive maintenance

6.1.2 Cleaning before maintenance or service

6.1.3 Maintenance schedules The table below provides recommendations for the frequency of regular maintenance procedures for columns in continuous use.

Make sure that the product file is kept updated and together with the product documentation. The file should contain information about inspections, main- tenance procedures that have been performed, spare parts that have been exchanged and any other relevant information for safe operation.

Note:

FrequencyProcedure

At least once every two yearsChange all O-rings

Every time O-rings are changedClean O-ring grooves

Every third month, but preferably before each packing

Change liquid in flushing channels

RegularlyCheck all seals for leakage

Every 5-10 packings, more frequently if re- quired

Change O-rings and seals on moving parts (e.g. adapter and media valve)

Every 5-10 packings, more frequently if re- quired

Clean stainless steel bed supports

Every 5-10 packings, more frequently if re- quired

Change plastic bed supports

Each time the bed support is fittedChange plastic bed support snap ring

Before bed support assembly if the grip/threads are damaged

Change bed support nuts1

1 Only applicable for AxiChrom 1200-1600 columns with plastic bed supports.

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6 General maintenance and service 6.1 Service and preventive maintenance 6.1.3 Maintenance schedules

6.2 General maintenance tools This section describes general tools required for the maintenance of all sizes of AxiChrom columns.

Tool kit General tools required for disassembly, maintenance and assembly of AxiChrom columns are provided in an AxiChrom toolkit, available from GE. For further details, see Section 12.7 Ordering information, on page 328.

O-ring removal tool The removal tool is used for removing O-rings from their grooves and for similar tasks. The tool is made of plastic to avoid scratching O-ring fittings. The image below shows how to use the removal tool to remove the snap ring used with plastic bed supports.

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6 General maintenance and service 6.2 General maintenance tools

Plastic mallet The plastic mallet is used to tap the snap ring gently into position when fitting plastic bed supports. The groove on the mallet head may be used to lever the snap ring over the edge of the bed support if the fit is tight. The image below shows how to use the mallet head to lever the snap ring into position.

PTFE tape It is recommended to wrap PTFE tape around the threads of all bolts and screws of size M8 and above, with the exception of bolts that go into a threaded insert.

In maintenance procedures that involve the removal of bolts and screws with PTFE tape, remove the old tape from the bolts and apply new tape before refitting the bolts and attaching the nuts. Apply the tape in the same direction as the threads so that refitting the nuts draws the tape into the threads.

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6 General maintenance and service 6.2 General maintenance tools

6.3 Power failure during maintenance If there is a power failure during any column operation controlled via the AxiChrom 300-1600 Master, information about the ongoing procedure will be lost. However during maintenance mode the last static position is remembered so that the maintenance procedure can be resumed. After the AxiChrom 300-1600 Master has been restarted, start the sameMaintenancewizard again to carry on from the correct step. The column has to be re-calibrated if the power is lost during any adapter movement.

NOTICE If the wrong procedure is started, the on-going procedure will be reset and the column must be assembled or disassembled manu- ally. If this becomes necessary, contact your GE representative.

1 Restart the AxiChrom 300-1600 Master.

2 The AxiChrom 300-1600 Master will automatically detect the correct column from the Select column list. Click Continue.

3 Verify the actual distance read from the level scale on the adapter rod. Enter the correct value in the Verify adapter position dialog.

The illustration below shows the adapter rod with its level scale. Note that the scale runs downward.

NOTICE When power is restored it is very important that the correct position of the adapter is verified, otherwise column damage may result.

If the wrong adapter position is used, the result of future packings will be incorrect, maintenance positions will be incorrect and it may not be possible to complete the maintenance procedures.

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6 General maintenance and service 6.3 Power failure during maintenance

See Section 5.2 Starting up and shutting down the AxiChrom300-1600Master, on page82 and the AxiChrom User Manual for further information about calibration and restarting.

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6 General maintenance and service 6.3 Power failure during maintenance

6.4 Putting the column in and out of maintenance mode

Introduction The AxiChrom 300-1600 MasterMaintenancewizards are used for the disassembly and assembly of the column, for example to access the inside of the column to replace bed supports and O-rings. For AxiChrom 1200-1600 columns there are also wizards for lifting the bottom backing plate to facilitate media valve maintenance, see Section 8.3 Lifting and lowering the bottom backing plate, on page 215.

The wizard contains step-by-step instructions for the disassembly and assembly proce- dures and also allows control of the necessary adapter movements. The wizard dialogs contain safety information, warnings and cautions which are displayed in connection to the process steps. Ensure that all locking pins and lock blocks are used as directed in the wizard screen.

The procedures for column disassembly and assembly are described more in detail in the following chapters. Refer to these instructions for an overview before starting to disassemble the column.

Follow all safety instructions carefully so as to avoid risk of injury during column disas- sembly and assembly procedures, especially when the adapter is in motion outside the column tube.

If aMaintenancewizard has been started, it is important to step through the whole procedure. If aMaintenancewizard has for some reason been aborted, start the samewizard again and finalize the procedure. If thewrong procedure is started, the on-going procedurewill be reset and the columnmust be assem- bled or disassembled by running the AxiChrom 300-1600 Master in manual mode. If this becomes necessary, contact your GE representative.

Note:

Safety information

WARNING Follow all safety instructions displayed on the AxiChrom Master interface screen when performing maintenance on the column.

WARNING Make sure that all locking pins and lock blocks are mounted in their proper, marked positions before proceeding with the next step in the column maintenance procedure.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

CAUTION The air from damaged pneumatic tubes may cause injuries. Replace any damaged tubes.

NOTICE Lifting the top unit with incorrectly placed lock blocks may damage the column.

NOTICE The stand tubes of AxiChrom 300-1000 columns will lock if the top unit is lifted above the permitted height. Please contact your GE service operator for help to resolve this.

Risk areas The illustration below shows the column areas where body parts may get caught when the adapter is lowered into the column tube and the suspended column tube is lowered back into position after maintenance. Although the adapter movement is very slow, the force generated by the adapter control motor is substantial and any item that is caught between the moving adapter and other surfaces will be severely damaged.

The column tube swings out to offer access to the bed supports. The column tube must be locked in place using the designated locking pin when it is in its outer position.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

Column disassembly wizard

TheMaintenancemenu wizard dialog screens that the user sees are slightly different for AxiChrom300-1000 columns andAxiChrom1200-1600 columns. This is due to the extramaintenancewizard steps that allow the user to perform media valve maintenance on AxiChrom 1200-1600 columns.

Note:

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

In the AxiChrom 300-1600 Master Main menu, select Maintenance. This disassembly wizard is interactive and provides the user with instructions about what to do and when.

Result: The maintenance dialog screen that appears next will depend on the column size.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

• If maintenance is performed on an AxiChrom 300-1000 column, select Disassembly in the column maintenance dialog to open the wizard that sets the column into maintenance mode.

• If maintenance is performed on an AxiChrom 1200-1600 column, select Column in the first dialog screen that appears.

Then selectDisassembly in the next column maintenance dialog to open the wizard that sets the column into maintenance mode.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Precheck: Make sure that the inlet and outlet flow paths are open and that the column is fully depressurized (for example by disconnecting the Top mobile phase hose). Press Confirm.

CAUTION A sudden release of pressure when disconnecting tubing may cause injury.

Precheck: Verify that the four locking pins and all lock blocks are in their storage positions as illustrated below. The illustration shows four blocks. The number of blocks differs between column sizes.

Press Confirm.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Unscrew and remove the nuts from the top column flange bolts as illustrated below (note that the number of bolts differs between column sizes).

NOTICE Be careful not to damage the column tube when re- moving the nuts. Use the closed end of the wrench to remove the nuts.

Press Confirm.

Verify that all top flange nuts are removed. Press Confirm to move the adapter to the top position.

Result: The adapter moves to its top position.

NOTICE If all nuts are not removed, the column may be dam- aged in later steps.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Remove the lock blocks from their storage positions. Fit the lock blocks in the lock positions as illustrated below (the illustration shows four blocks in lock positions 1A, 1B, 1C and 1D: the number of blocks differs between col- umn sizes). Tighten the bolts securely to 45 Nm, using a torque wrench. Press Confirm.

NOTICE Do not use force to fit lock blocks. Lock blocks that are difficult to screw into place may be damaged and must be replaced.

Verify that the lock blocks are securely fitted. Press Confirm to start lifting the top unit assembly.

Result: The top unit now moves upward.

NOTICE Lifting the top unit with incorrectly placed lock blocks may damage the column.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Insert and secure the locking pins in the lock hole positions 2A and 2B, as illustrated below.

NOTICE Moving the adapter with only one locking pin in place may damage the column.

If the locking pins cannot be inserted into the lock holes for 300-600 columns, the top unit may not be properly seated on the lock blocks as shown below.

Shake the top unit gently by hand until the adapter rests securely on the lock blocks, as shown below.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Verify that the locking pins are correctly inserted and secured in position. Press Confirm to move the adapter up to the chamfer, in the top of the col- umn tube, to release the compression of the adapter seals.

Result: The adapter moves up to the column tube chamfer.

Unscrew the nuts and remove the bottom flange bolts, using two wrenches (one on either side of the bolt and nut assembly). Press Confirm.

Verify that all bottom flange bolts are removed. Press Confirm to lift the column tube.

Result: The column tube now moves up.

NOTICE If all bottom flange bolts are not removed, the column may be damaged in later steps.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Insert and secure a locking pin in the lock hole position 3, as illustrated below. Press Confirm.

NOTICE Make sure to insert the safety peg into the locking pin so that the safety catch is at the bottom of the locking pin, as shown in the illustrations. Insertion in any other orientation may result in damage to the safety peg and locking pin.

1200-1600 columns300-1000 columns

Verify that the locking pin is secured and in the correct position. Press Con- firm to lower the adapter.

Result: The adapter moves down to release the weight from the lock blocks.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Remove the lock blocks from their lock positions and place the blocks in their storage positions. Press Confirm.

Verify that all lock blocks are removed and in their storage positions. Press Confirm to move the adapter to the top position.

Result: The adapter now moves to its top position.

Swing out the column tube to perform the maintenance operations. Use the remaining locking pin to secure the column tube in the maintenance position.

CAUTION Make sure that no body parts become trapped be- tween the column tube assembly and the column frame when using the swing out function for mainte- nance work on the column. The column tube must be locked in place during all maintenance work. Due to the weight of the column tube assembly, it is not advis- able to try to stop the momentum of the assembly by hand when it is set in motion.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

If you are going to perform maintenance operations related to the media valve on a 1200, 1400 or 1600 sized column, you have to lift the bottom backing plate to be able to access the media valve. See Lifting the bottom backing plate, on page 215.

Column assembly wizard

ActionStep

If you have performed maintenance operations related to the media valve on a 1200, 1400 or 1600 sized column, you have to lower the bottom backing plate before starting the assembly. See Lowering the bottom backing plate, on page 223.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

When the maintenance operations are completed, go to the AxiChrom Master Main menu, select Maintenance, and then select Assembly in the column maintenance dialog if your are working with a AxiChrom 300-1000 column.

If working with AxiChrom 1200-1600 columns, selectMaintenance from the Main menu, and then select Column in the first dialog screen that appears.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Then select Assembly in the next column maintenance dialog.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Press Confirm.3

Result: The adapter will move to the top position if it is not already in the top position.

Note:

The adapter will already be in the top position if the adapter has not been moved after column disassembly.

Precheck: Make sure that the inlet and outlet flow paths are open. Press Confirm.

Verify that the lock blocks are in their storage positions as illustrated below. The illustration shows four blocks. The number of blocks differs between column sizes. Press Confirm.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Remove the locking pin 4, that secures the column tube in maintenance position. Press Confirm.

Swing the tube in position for operation. Press Confirm.7

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Insert bottom flange bolts, with their corresponding washers, to align the tube with the corresponding holes in the column bottom lid, as illustrated below. Do not refit the nuts yet. Press Confirm.

Verify that all bottom flange bolts are inserted and that the column tube is properly aligned. Press Confirm to move the adapter.

Result: The adapter moves down into position so that the lock blocks can be fitted.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Remove the lock blocks from their storage positions. Fit the lock blocks in the lock positions as illustrated below (the illustration shows four blocks in lock positions. The number of blocks differs between column sizes. Tighten the bolts firmly. Wet the scraper seals with 20% ethanol. Press Confirm.

NOTICE Lifting the column tube with incorrectly fitted lock blocks may damage the column.

NOTICE Do not use force to fit lock blocks. Lock blocks that are difficult to screw into place may be damaged and must be replaced.

Verify that the lock blocks are securely fitted. Press Confirm to start lifting the column tube.

Result: The column tube moves upward.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Remove the locking pin from lock hole position.12

Place the locking pin in storage position as illustrated below.

Press Confirm.

NOTICE Moving the column tube with a locking pin in the wrong place may damage the lock blocks.

Verify that the locking pin is removed and placed in storage position. Press Confirm to lower the column tube.

Result: The column tube moves downward.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Fit the flange nuts and washers to the bottom flange bolts, and finger tighten the nuts. Press Confirm.

Verify that all bottom flange bolts are properly fitted. Press Confirm to lift the top unit assembly.

Result: The top unit moves upward.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Remove the locking pins from lock hole positions 2A and 2B.16

Place the locking pins in their storage positions as illustrated below.

Press Confirm.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Tip:

If the locking pins are difficult to remove, the top unit may not be properly seated on the lock blocks. If this happens for smaller columns with compara- tively lightweight top units (300-600 column sizes only), shake the top unit gently by hand until the adapter rests securely on the lock blocks, as shown in the image.

Verify that the locking pins are removed from both positions and placed in their storage positions. Press Confirm to lower the top unit.

Result: The top unit moves downward to position.

Remove the lock blocks from their lock positions and place the blocks in their storage positions under the top flange as illustrated below The illustra- tion shows four blocks. The number of blocks differs between column sizes. Press Confirm.

NOTICE Moving the adapter with a lock block in the wrong place may damage the column.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Verify that the threads of the top flange bolts are wrapped with PTFE tape and that all lock blocks are removed and in their storage positions. Press Confirm.

Fit the washers and nuts to the top flange bolts as illustrated below. Finger tighten the nuts. Press Confirm.

Verify that all top flange bolts are properly fastened. Press Confirm to lower the adapter.

Result: The adapter is pressing the column tube downward.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

Using a calibrated torque wrench, tighten the bottom flange nuts, crosswise and in increments, to the correct tightening torque, according to the following torque table. Press Confirm.

Tightening torque [Nm] for flange nuts Stainless steel tubes

Tightening torque [Nm] for flange nuts Acrylic tubes

Column size

8080300

8080400

8080450

100100600

140140800

1401401000

1801801200

3003001400

3003001600

Verify that all bottom flange bolts are properly tightened. Press Confirm to lower the top unit.

Result: The adapter pulls the top unit downward.

Using a calibrated torque wrench, tighten the top flange nuts to the correct tightening torque (refer to the torque table in step 22, above). PressConfirm.

Verify that all top flange bolts are properly tightened. Press Confirm.25

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

ActionStep

The column is now assembled. Press Continue to complete the AxiChrom column Maintenance/Assembly wizard.

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6 General maintenance and service 6.4 Putting the column in and out of maintenance mode

6.5 Media valve maintenance

Introduction Media valve maintenance is performed in connection with bed support maintenance.

To perform maintenance or service operations the column must be in maintenance mode. Follow the instructions in Section 6.4 Putting the column in and out ofmaintenance mode, on page 115 to set the column in maintenance mode.

To perform media valve maintenance on AxiChrom 1200-1600 columns, the bottom backing plate must be raised after the column has been set in maintenance mode, see Section 8.3 Lifting and lowering the bottom backing plate, on page 215.

Disassembling the media valve Make sure the column is empty before disassembling the media valve.

O-rings that form stationary seals in the media valve should be replaced at least once every two years. O-rings on or adjacent to moving parts should be replaced after every 5-10 packings, or more frequently if necessary.

At least two persons are needed when disassembling the media valve.Note:

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Set the media valve to Open in the Manual Control wizard in the AxiChrom 300-1600 Master.

F F

AB C D

FunctionPart

Valve pneumatic inletsA

Slurry inletB

Bottom mobile phase inlet/outletC

Rinse inletD

Media valve bolts screwed into the bottom backing plateE

Locking screws on the tube holder boltsF

Disconnect the air supply tubes from the two valve pneumatic inlets (A) on the media valve.

Note:

Make sure tomark each of the supply tubes before disconnection, for example with a piece of tape, so that these can be reconnected correctly.

Disconnect the hoses to the:

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

• Slurry inlet (B),

• Bottom mobile phase inlet/outlet (C), and

• Rinse inlet (D)

Hold the media valve in position while removing the four bolts screwed into the valve from underneath the column (E).

Note:

Be aware that the residual liquid remaining in the media valve will spill out when the bolts are removed.

Continue to hold the media valve in place while loosening the two locking screws on the tube holder bolts (F).

The media valve and tube holder can now be removed downwards from the column. Let the tube holder slide off its fastener. The following steps on the media valve can be performed on a table.

Firmly press the release washer and hold it into the connector while removing the pneumatic tube from pneumatic cylinder (1). Repeat the procedure to disconnect the other pneumatic tube (2).

2 1

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Unscrew and remove the tube holder brackets (B) from the:6

• Slurry inlet tube,

• Bottom mobile phase tube, and

• Rinse inlet tube.

Let the pneumatic tubes stay connected to the tube holder (A).

BB B

Remove the tube holder and pneumatic tubes from the media valve.

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Remove the three pipings and their O-rings from the media valve body:7

• Slurry inlet (A),

• Bottom mobile phase inlet/outlet (B),

• Rinse inlet (C).

Clean the O-rings with 20% ethanol. Replace with new O-rings if necessary.

A CB

For 300-600 columns equipped with plastic tubes, the rinse inlet has an inner connector and additional O-ring.

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Unscrew the four bolts to remove the pneumatic cylinder and its piston from the media valve outer body.

On AxiChrom 300-600 columns: When removing the piston, hold the upper part of the pneumatic cylinder, to prevent the cylinder from falling apart.

Loosen the four bolts on the bottom of the media valve outer body. Do not remove the bolts. Make sure a few millimeters of the bolt remain screwed into the media valve. Press the bolts against a flat surface so that the bolts push out the valve inner body, then remove the valve inner body.

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Remove and clean the O-ring from the valve outer body with 20% ethanol, or replace with a new O-ring if necessary.

Remove and clean the four O-rings from the valve inner body with 20% ethanol, or replace with new O-rings if necessary.

Assembling the media valve At least two persons are needed when assembling the media valve.Note:

ActionStep

Refit all O-rings and the O-ring holder on the valve inner body.1

When refitting the diagonal O-ring on the valve inner body, make sure to stretch it when putting it back in place. It must not be rolled into place.

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Wet the diagonal O-ring with 20% ethanol, then assemble the media valve inner body into the valve outer body. Make sure that the media valve inner body pin fits inside the hole on the bottom of the valve outer body. Tighten the bolts (crosswise) in steps up to 2.2 Nm.

Look into the mobile phase inlet/outlet and make sure that no part of the diagonal O-ring can be seen. If any part of the diagonal O-ring is visible it is likely that the valve will leak. If the O-ring is visible, undo the bolts again and repeat step 2.

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Refit the pneumatic cylinder into the media valve outer body.4

Make sure that the alignment of the pneumatic cylinder corresponds to the image below. Tighten the bolts (crosswise) in steps to 1.7 Nm.

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Reposition the slurry inlet, rinse inlet and bottom mobile phase inlet/outlet tubes on the media valve. Tighten the bolts enough to hold the tubes firmly in position, but do not tighten the bolts fully at this stage.

CAUTION The air from damaged pneumatic tubes may cause injuries. Replace any damaged tubes.

A CB

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Place the media valve so that the mobile phase tube rests in the correct position in the tube holder (A). Refit the tube holder bracket (B) on the mobile phase tube and tighten the bolts slightly, so that the tube can still slide under the bracket, to allow for adjustments in position when the tube holder is re- fitted to the bottom backing plate.

BB B

Reposition the Slurry inlet tube (A) and Rinse inlet tube (C) against the media valve body and tighten the tube bolts enough to hold the tubes firmly in position, but do not tighten the bolts fully until verifying that the tubes have been refitted at their correct angles.

Note:

To ensure that the Slurry inlet tube and the Rinse inlet tube are placed in their correct angle when refitted, assemble the tube holder brackets, but tighten the bolts only slightly, so that the tubes can still slide under the brackets.

A CB

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

When all of the media valve tubes are correctly in position, tighten all of the bolts holding the tubes to the media valve to 2.2 Nm.

For AxiChrom 1200-1600 columns: It is recommended to perform a media valve leakage test at this stage, using the leakage test unit that is available for this purpose (optional accessory).

Refit the two pneumatic tubes on the pneumatic cylinder according to the image below. Make sure that the two tubes are connected to the correct connectors.

2 1

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6 General maintenance and service 6.5 Media valve maintenance

ActionStep

Re-assemble the media valve and tube holder under the column.10

F F

AB C D

FunctionPart

Valve pneumatic inletsA

Slurry inletB

Bottom mobile phase inlet/outletC

Rinse inletD

Media valve bolts screwed into the bottom backing plateE

Locking screws on the tube holder boltsF

1 Assemble the four media valve bolts into the bottom backing plate. Tighten the bolts (crosswise) in steps up to 5 Nm.

2 Tighten the bolts on the tube holder brackets. The bolts should be tight- ened evenly and not too hard, to avoid any damage to the tubes, espe- cially if plastic tubes are used.

3 For media valves equipped with plastic tubes, make sure the tubes are at a slight angle to the horizontal so that liquid in the tubes drains away from the media valve.

4 Tightening the locking screws on the tube holder bolts to 10 Nm.

Connect the air supply tubes to the two valve pneumatic inlets on the tube holder. Open the air supply to the column.

Connect the hoses to the Slurry inlet, Bottom mobile phase inlet/outlet , and Rinse inlet. See Section 4.6 Connections, on page 68 for more information.

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6 General maintenance and service 6.5 Media valve maintenance

6.6 Changing liquid in the flushing channels AxiChrom column flushing channels should always be filled with 20% (v/v) ethanol. It is recommended to change the liquid in the flushing channels at frequent intervals (at least once every three months), and preferably after each new packing.

AxiChrom column flushing channels are filled with 20% (v/v) denatured alcohol on delivery. Flush the delivery solution out of the flushing channels as soon as possible after delivery, preferably within the first two to three months, and replace with 20% (v/v) ethanol.

Flushing ports The flushing channels of the column are connected to flushing ports that are located around the circumference of the adapter. The flushing ports are used to flush liquid through the flushing channels.

At the other end of the tubing that is connected to the flushing port/flushing connection there is a small valve that is sometimes referred to as the SRV valve or flush valve. A Luer-Lock™ connection is attached to this valve that can be used for connecting to a syringe.

The number of flushing ports on AxiChrom columns is dependant on column size. The largest AxiChrom column sizes have four flushing ports.

Procedure To change the liquid in the flushing channels, work systematically around the column, flushing liquid through one flushing port at a time.

The procedure for AxiChrom 1200-1600 columns is described here, but the principle of working systematically around the column is applicable for all column sizes.

Note:

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6 General maintenance and service 6.6 Changing liquid in the flushing channels

ActionStep

Open the Luer connector on the tube of one of the flushing ports (for example, flushing port A in the illustration of the locations of the flushing ports on AxiChrom 1200-1600 columns).

Connect a syringe filled with 20% (v/v) ethanol to the Luer connector on the tube of one of the two adjacent flushing ports (for example, port D).

Push fluid into the flushing port (port D) and through the flushing channel until the fluid in the flushing channel has been replaced and fluid flows steadily out from the first flushing port (port A).

Close flushing port D.4

Move around the column and open the Luer connector on the tube of the next flushing port along (flushing port B).

Connect a syringe filled with 20% (v/v) ethanol to the Luer connector on the tube of flushing port A.

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6 General maintenance and service 6.6 Changing liquid in the flushing channels

ActionStep

Push fluid into flushing port A and through the flushing channel until the fluid in the flushing channel has been replaced and fluid flows steadily out from flushing port B.

Close flushing port A.8

Move around the column and open the Luer connector on the tube of the next flushing port along (flushing port C).

Connect a syringe filled with 20% (v/v) ethanol to the Luer connector on the tube of flushing port B.

Push fluid into flushing port B and through the flushing channel until the fluid in the flushing channel has been replaced and fluid flows steadily out from flushing port C.

Close flushing port B.12

Move around the column and open the Luer connector on the tube of the next flushing port along (flushing port D).

Connect a syringe filled with 20% (v/v) ethanol to the Luer connector on the tube of flushing port C.

Push fluid into flushing port C and through the flushing channel until the fluid in the flushing channel has been replaced and fluid flows steadily out from flushing port D.

Close flushing ports C and D.16

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6 General maintenance and service 6.6 Changing liquid in the flushing channels

6.7 Changing flushing connector O-rings There are two flushing connectors for the 300-1000 columns and four flushing connectors for the 1200-1600 columns, on the top of the adapter backing plate. Inside each flushing connector are two O-rings, which should be changed at least once every two years. The O-rings should be checked regularly and cleaned if needed.

Follow the instruction below to change the flushing connector O-rings.

ActionStep

Unscrew the flushing connector retaining nut. The image below is a cross- section showing (1) the flushing connector retaining nut, (2) the flushing connector, and the two O-rings indicated by arrows.

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6 General maintenance and service 6.7 Changing flushing connector O-rings

ActionStep

The flushing connector (2) is threaded into the adapter distributor. Unscrew the flushing connector so that the O-rings can be reached.

Remove the two O-rings from the flushing connector. Clean the O-rings with 20% ethanol or replace with new O-rings if necessary.

Refit the flushing connector (2) on the distributor plate. Tighten finger-tight, and then tighten a further 25 degrees using a wrench.

Refit the flushing connector retaining nut (1) and tighten finger-tight.5

Repeat steps 1-5 on the other flushing connector.6

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6 General maintenance and service 6.7 Changing flushing connector O-rings

6.8 Leakage test

Perform a leakage test according to Section 5.4 Performing a leakage test, on page 90 when:

• the column is new,

• the column has been shipped,

• the O-rings have been replaced, or

• other parts that may cause leakage have been replaced,

• maintenance has been performed,

• the column has been taken out of storage.

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6 General maintenance and service 6.8 Leakage test

7 Maintenance of 300-1000 columns

About this chapter This chapter provides instructions that are specific for performing maintenance proce- dures on AxiChrom 300-1000 columns.

General maintenance procedures that are performed in the same way for all column sizes are described in Chapter 6 General maintenance and service, on page 106.

In this chapter

See pageSection

1627.1 Maintenance tools for 300-1000 columns

1637.2 Maintaining the adapter inlet/outlet

1667.3 Maintaining columns with stainless steel bed supports

1817.4 Maintaining columns with plastic bed supports

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7 Maintenance of 300-1000 columns

7.1 Maintenance tools for 300-1000 columns This section describes tools that are used specifically when performing service and maintenance on AxiChrom 300-1000 columns. General tools used with all column sizes are described in Section 6.2 General maintenance tools, on page 111.

NOTICE Always use appropriate tools and the correct tightening torques for bolts when working with the AxiChrom column and AxiChrom Master unit. Only the special toolkit provided by GE for the AxiChrom column should be used.

Bed support tool The bed support tool is used for removing and replacing the bed support screw. Engage the pins on the tool firmly in the screw when using the tool.

The bed support tool for 300-1000 columns is shown below.

The threaded end of the tool is used with stainless steel bed supports to assist in centering the bed support on the distributor during assembly.

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7 Maintenance of 300-1000 columns 7.1 Maintenance tools for 300-1000 columns

7.2 Maintaining the adapter inlet/outlet The instructions for adapter inlet/outlet (top mobile phase inlet/outlet) maintenance applies only to AxiChrom 300-1000 columns. For AxiChrom 1200-1600 columns see Section 8.2 Rinse nozzle maintenance, on page 207.

Adapter inlet/outlet maintenance is performed in connection with bed support mainte- nance.

• For columns with stainless steel bed supports, see Section 7.3 Maintaining columns with stainless steel bed supports, on page 166.

• For columns with plastic bed supports, see Section 7.4 Maintaining columns with plastic bed supports, on page 181.

This section describes how to change the O-rings in the inlet/outlet valve.

DescriptionPart

Outer countersunk boltsA

Inner boltsB

ConnectorC

Valve outer bodyD

Plastic spacer and O-ring (only for 300, 400, 450 and 800 column sizes)

Valve inner bodyF

Valve body O-ringsG

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7 Maintenance of 300-1000 columns 7.2 Maintaining the adapter inlet/outlet

Disassembling the adapter inlet/outlet

ActionStep

Remove the connector (C) by removing the four bolts. Remove the O-ring from underneath the connector. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Loosen the four bolts (B) on the top of the adapter inlet/outlet but do not fully remove the bolts at this stage. Unscrew the bolts a few turns, until each bolt protrudes from the valve inner body by the same distance. Press the protruding bolts against a flat surface so that the bolts push out the valve inner body, then unscrew the bolts completely and remove the valve inner body from the inlet/outlet assembly.

NOTICE Make sure the bolt heads sit evenly against the flat surface so that the load is evenly distributed over the four bolt heads.

Remove the four O-rings (G) from the valve inner body and from underneath the inlet/outlet. Clean the O-rings with 20% ethanol or replace them with new O-rings if necessary.

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7 Maintenance of 300-1000 columns 7.2 Maintaining the adapter inlet/outlet

Assembling the adapter inlet/outlet

ActionStep

Refit the diagonally placed O-ring on the valve inner body.

Note:

Stretch the O-ring when refitting it. Do not roll it into place.

Refit the O-ring on top of the valve inner body.2

Wet the inner body O-rings thoroughly, as well as the surface of the inner surface of the outer body, and then refit the valve inner body and tighten the four bolts (B) crosswise to 2.2 Nm.

Refit the connector O-ring and the connector (C). Tighten the four bolts crosswise to 2.2 Nm.

Refit the remaining O-rings.5

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7 Maintenance of 300-1000 columns 7.2 Maintaining the adapter inlet/outlet

7.3 Maintaining columns with stainless steel bed supports

Introduction This section describes the maintenance procedures for columns with stainless steel bed supports.

For the maintenance schedule recommendations, please see Section 6.1.3 Maintenance schedules, on page 110.

In this section

See pageSection

1687.3.1 Disassembling the bottom assembly

1707.3.2 Disassembling the adapter

1757.3.3 Assembling the adapter

1797.3.4 Assembling the bottom assembly

Precautions

NOTICE Disassemble the bottom assembly first, so that components that may be dropped when working on the adapter assembly will not damage the fragile bed support. Similarly, assemble the adapter assembly before working on the the bottom assembly.

NOTICE Do not exceed stated torque settings. Overtightening may cause damage.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

NOTICE Handle the bed supports with care. The bed supports are fragile.

Always use protective gloves when handling the bed supports to avoid contaminating the bed supports.

NOTICE Make sure the column is in maintenance mode before performing service operations. Follow the instructions in Section 6.4 Putting the column in and out ofmaintenancemode, on page115 to set the column in maintenance mode if required.

NOTICE It is recommended to wrap PTFE tape around the threads of all bolts and screws of size M8 and above, with the exception of bolts that go into a threaded insert.

NOTICE Damage to the equipment may occur if the steps in the mainte- nance instructions are not followed.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

7.3.1 Disassembling the bottom assembly

Removing the bed support

ActionStep

Make sure that the media valve is open. The media valve is open when the piston in the center of the bed support is lowered. If the media valve is closed, it can be opened using the Manual Control wizard in AxiChrom 300-1600 Master.

Use the bed support tool to remove the bed support screw in the center.2

NOTICE Be careful not to slip with the bed support tool since this may damage the bed support screw and/or the bed support.

NOTICE Be careful not to damage the threads when removing the bed support screw.

Remove the O-ring on the bed support screw. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports 7.3.1 Disassembling the bottom assembly

ActionStep

Unscrew the nuts underneath the bottom backing plate so that the bottom bed support can be lifted. Screw the eye bolts (A) delivered with the column into the holes and use them to lift the bed support by hand. The number of eye bolts differs depending on the column size.

Remove the large O-ring (B) from the outer edge of the bed support. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Remove the two small O-rings from center of the bed support.

Tip:

The two small O-rings in the center of the bed support are located one on each side of the bed support. The lower O-ring is under the bed support and must be removed after the bed support has been lifted off from the bottom assembly.

Clean the O-rings with 20% ethanol or replace them with new O-rings if necessary.

Remove the O-ring from the distributor plate. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

7.3.1 Disassembling the bottom assembly

7.3.2 Disassembling the adapter At least two persons are needed when disassembling the adapter assembly.Note:

Removing the bed support

ActionStep

Use the bed support tool to remove the bed support screw from the center of the bed support. Hold the bed support tool vertically when removing the bed support screw.

NOTICE Be careful not to damage the threads when removing the bed support screw.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports 7.3.2 Disassembling the adapter

ActionStep

With the weight of the bed support assembly supported, unscrew the nuts and remove the bed support assembly.

Tip:

Leave a couple of the nuts on opposite sides partly unscrewed. This will de- crease the risk that the bed support assembly falls down by accident.

With the weight supported and at the same time gently pressing on the partly unscrewed nuts might help to remove the bed support assembly if stuck.

Note:

The nuts should release from the fasteners, leaving the fasteners attached to the bed support. If any fastener releases from the bed support, refit the fas- tener and tighten to a torque of 20 Nm.

Place the bed support on a clean, protective surface.3

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

7.3.2 Disassembling the adapter

ActionStep

Remove the O-ring in the center of the top side of the bed support. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Remove the scraper seal and its underlying O-ring from the bed support.5

Remove the O-ring in the center of the bottom side of the bed support.

Clean the scraper seal and O-rings with 20% ethanol or replace them with new seal and O-rings if necessary.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports 7.3.2 Disassembling the adapter

ActionStep

Remove the O-ring from the distributor plate. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Removing the adapter inlet/outlet

ActionStep

Remove the adapter inlet/outlet from the column by unscrewing the four outer countersunk bolts.

Note:

The 300, 400, 450, and 800 columns have a plastic spacer placed between the adapter inlet/outlet and the column (indicated by arrows in the illustration). Remove this plastic spacer and the O-ring underneath. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Replace the O-rings in the inlet/outlet valve if necessary (see Section 7.2 Maintaining the adapter inlet/outlet, on page 163).

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

7.3.2 Disassembling the adapter

Removing the distributor plate

ActionStep

Remove the two flushing connectors according to the instruction in Sec- tion 6.7 Changing flushing connector O-rings, on page158. Clean their O-rings with 20% ethanol or replace them with new O-rings if necessary.

Remove the four bolts underneath the inlet/outlet.2

With the weight of the distributor plate supported, unscrew the bolts and remove the distributor plate. The number of bolts varies with column size.

Remove the scraper seal and the large O-ring around the distributor plate. Inspect for damage. Clean the scraper seal and O-ring with 20% ethanol or replace them if necessary.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports 7.3.2 Disassembling the adapter

7.3.3 Assembling the adapter At least two persons are needed when assembling the adapter.Note:

Refitting the distributor plate

ActionStep

Wet the large O-ring and scraper seal with 20% ethanol, then refit the O- ring on the distributor plate and place the scraper seal on top of the O-ring.

Refit the distributor plate. Ensure that the holes in the distributor plate are aligned with the flushing connectors as illustrated below. First, refit all bolts finger-tight on the distributor plate. Then use a torque wrench to tighten the M12 bolts on the distributor plate to 15 Nm and the four bolts underneath the inlet/outlet assembly to 11 Nm.

Refit the two flushing connectors according to the instructions in Section 6.7 Changing flushing connector O-rings, on page 158.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

7.3.3 Assembling the adapter

Refitting the inlet/outlet

ActionStep

Refit the plastic spacer and O-ring (if used) on the inlet/outlet. The plastic spacer is used on the 300, 400, 450 and 800 columns, and not on the 600 and 1000 columns. In the image below, arrows indicate the plastic spacer and O-ring.

Refit the adapter inlet/outlet to the column. Make sure that the O-rings are positioned correctly. Refit the inlet/outlet so that the tubing is closest to the tube roller assembly. Tighten the four bolts crosswise to 5 Nm.

NOTICE When fitting an old inlet/outlet on a newer column, check if there are more than four threaded holes that the inlet/outlet can be fitted to on top of the adapter. If so, remove the guide pin from the inlet/outlet if it exists.

Guide pin

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports 7.3.3 Assembling the adapter

Refitting the bed support

ActionStep

Refit the two center O-rings, one on each side of the bed support.1

Refit the bed support O-ring and scraper seal.2

While holding the bed support in place, refit the bed support fastening nuts, and their associated plastic and stainless steel washers, so that the bed support is retained. Do not tighten the nuts yet.

Wet the threads of the bed support tool, and then use the threaded side of the bed support tool to center the bed support on the distributor plate. Make sure that the center of the bed support exactly fits into the corresponding countersink in the distributor plate, then tighten the bed support fastening nuts fingertight, while the bed support tool is still in place.

Tighten the bed support fastening nuts using a torque wrench to tighten all of these nuts crosswise to a torque of 10 Nm for 300-600 columns, or 15 Nm for 800 and 1000 columns.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

7.3.3 Assembling the adapter

ActionStep

Remove the bed support tool, wet surfaces with 20% ethanol and refit the bed support screw. Carefully start to tighten the bed support screw by hand making sure that the threads are not crossed. Finally tighten the bed support screw firmly using the bed support tool. Make sure the bed support is secured by the bed support screw.

NOTICE Be careful not to damage the threads when refitting the bed support screw.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports 7.3.3 Assembling the adapter

7.3.4 Assembling the bottom assembly ActionStep

Refit the O-ring on the distributor plate.1

Refit the O-ring on the bed support (B).2

Refit the two small O-rings on the center of the bed support, one on each side.

Refit the bed support on the distributor plate. Wet the threads of the bed support tool, and then use the threaded side of the bed support tool to fit and center the bed support on the distributor plate. Ensure that the center of the bed support fits exactly into the corresponding countersink in the distributor plate.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports

7.3.4 Assembling the bottom assembly

ActionStep

Leave the bed support tool in place while you refit the nuts under the backing plate. Tighten the nuts crosswise and in steps using a torque wrench, to 10 Nm torque for the 300-600 columns and 15 Nm for the 800 and 1000 columns.

Note:

The bed support fastening nuts can loosen when the tube is fastened into place. Check torque tensions and retighten to 10 Nm for 300-600 columns, or 15 Nm for 800-1000 columns, as required.

Make sure the bed support is secured by the bed support screw.

NOTICE Be careful not to damage the threads when refitting the bed support screw.

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7 Maintenance of 300-1000 columns 7.3 Maintaining columns with stainless steel bed supports 7.3.4 Assembling the bottom assembly

7.4 Maintaining columns with plastic bed supports

Introduction This section describes the maintenance procedures for columns with plastic bed supports.

For the maintenance schedule recommendations, please see Section 6.1.3 Maintenance schedules, on page 110.

In this section

See pageSection

1837.4.1 Disassembling the bottom assembly

1857.4.2 Disassembling the adapter

1917.4.3 Assembling the adapter

1977.4.4 Assembling the bottom assembly

Precautions

NOTICE Do not exceed stated torque settings. Overtightening may cause damage.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

NOTICE Handle the bed supports with care. The bed supports are fragile.

Always use protective gloves when handling the bed supports to avoid contaminating the bed supports.

NOTICE It is recommended to wrap PTFE tape around the threads of all bolts and screws of size M8 and above, with the exception of bolts that go into a threaded insert.

NOTICE Damage to the equipment may occur if the steps in the mainte- nance instructions are not followed.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.1 Disassembling the bottom assembly ActionStep

Use the bed support tool to remove the bed support screw in the center.2

NOTICE Be careful not to damage the threads when removing the bed support screw.

Remove the O-ring on the bed support screw.3

Clean the O-ring with 20% ethanol or replace it with a new O-ring if neces- sary.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.1 Disassembling the bottom assembly

ActionStep

Remove the bed support by hand. If necessary, insert the removal tool be- tween the edge of the bed support and the O-ring and gently lever up the bed support out of its seating.

Remove the O-ring from the distributor plate. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports 7.4.1 Disassembling the bottom assembly

7.4.2 Disassembling the adapter At least two persons are needed when disassembling the distributor plate.Note:

Removing the adapter inlet/outlet

ActionStep

Remove the adapter inlet/outlet from the column by unscrewing the four outer countersunk bolts. Arrows in the image below indicate the plastic spacer and O-ring on 300, 400, 450 and 800 columns.

The 300, 400, 450, and 800 columns have a plastic spacer placed between the adapter inlet/outlet and the column. Remove this plastic spacer and the O-ring underneath. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Replace the O-rings in the inlet/outlet valve if necessary (see Section 7.2 Maintaining the adapter inlet/outlet, on page 163).

Removing the bed support assembly

ActionStep

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.2 Disassembling the adapter

ActionStep

Remove the four bolts underneath the inlet/outlet.2

Remove the bolts on the top of the distributor plate. The number of bolts varies with columns size.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports 7.4.2 Disassembling the adapter

ActionStep

With the weight of the bed support assembly supported, unscrew the nuts and remove the bed support assembly.

Tip:

Leave a couple of the nuts on opposite sides partly unscrewed. This will de- crease the risk that the bed support assembly falls down by accident.

Note:

The nuts should release from the fasteners, leaving the fasteners attached to the distributor plate. If any fastener releases from the distributor plate instead, refit the fastener and tighten to a torque of 2 Nm.

Place the bed support and distributor assembly on a clean firm surface, al- lowing it to rest on the fasteners.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.2 Disassembling the adapter

ActionStep

Use the bed support tool to remove the bed support screw from the center of the bed support. Hold the bed support tool vertically when removing the bed support screw.

Remove the O-ring on the bed support screw. Clean the O-ring with 20% ethanol or replace with a new O-ring if necessary.

Disassembling the bed support assembly

ActionStep

Remove the snap ring using the removal tool.1

Lift the bed support from the distributor.2

Turn the distributor over so that the ribbed side faces downwards and place the distributor on a surface protected so as to avoid scratching the distributor channels.

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ActionStep

Remove the upper scraper seal and O-ring. Clean the seal and O-ring with 20% ethanol or replace with new components if necessary.

Remove the screws holding the distributor ring using an Allen key. Hexagon socket screws holding the distributor ring are illustrated below.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.2 Disassembling the adapter

ActionStep

Remove the distributor ring from the distributor.6

Remove the lower scraper seal and O-ring. Clean the seal and O-ring with 20% ethanol or replace with new components if necessary.

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7.4.3 Assembling the adapter At least two persons are needed when assembling the adapter.Note:

Reassembling the bed support assembly

ActionStep

Wet the O-ring and lower scraper seal with 20% ethanol. With the ribbed side of the distributor facing downwards, refit the O-ring on the distributor plate and place the scraper seal on top of the O-ring.

Place the distributor ring adapter on the distributor. The two steering pins ensure that the ring can only be placed in one orientation.

Refit the screws and washers to secure the distributor ring adapter. Tighten the screws with a torque wrench to 4 Nm. Hexagon socket screws holding the distributor ring are illustrated below.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.3 Assembling the adapter

ActionStep

Turn the distributor over so that it rests on the fasteners. Make sure that the lower scraper seal and O-ring remain in place.

Place the new bed support on the distributor. The smoother side of the bed support shall face the interior of the column.

Refit the O-ring in the bed support screw, then wet the O-ring and the bed support screw with 20% ethanol. Use the removal tool to help fit the O-ring if necessary.

Refit the bed support screw. Carefully start to tighten the bed support screw by hand making sure that the threads are not crossed. Finally tighten the bed support screw more firmly using the bed support tool. Make sure the bed support is secured by the bed support screw.

NOTICE Be careful not to damage the threads when refitting the bed support screw.

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ActionStep

Refit the snap ring, tapping gently with the plastic mallet if necessary. Use the groove on the mallet head to lever the snap ring over the edge of the bed support if the fit is tight.

Note:

Check that the snap ring is correctly and evenly mounted around the whole circumference of the bed support.

The illustration shows a cross-section of a snap-ring mounted correctly and two images of a snap-ring mounted incorrectly.

FunctionPart

Snap ringA

Scraper sealB

DistributorC

Bed supportD

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.3 Assembling the adapter

Refitting the bed support assembly

ActionStep

Wet the O-ring and upper scraper seal with 20% ethanol. Refit the O-ring on the distributor and place the scraper seal on top of the O-ring.

With the bed support and distributor assembly supported, refit the assembly on the backing plate. Position the assembly so that the holes in the assembly and backing plate are aligned (the assembly can only be mounted in one orientation on the backing plate). Fit both stainless steel and plastic washers onto each of the threaded bed support fasteners, with the plastic washer closest to the backing plate. Tighten all of the bed support fastening nuts finger-tight, then use a torque wrench to tighten all of these nuts crosswise to a torque of 4 Nm.

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ActionStep

Refit the bolts on top of the distributor plate. Fit both stainless steel and plastic washers on each screw, with the plastic washer closest to the backing plate. Tighten the bolts to 15 Nm.

A B

FunctionPart

Stainless steel washerA

Plastic washerB

Refit the four bolts underneath the inlet/outlet assembly and tighten to 11 Nm.

Refit the two flushing connectors according to the assembly instructions in Section 6.7 Changing flushing connector O-rings, on page 158.

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7 Maintenance of 300-1000 columns 7.4 Maintaining columns with plastic bed supports

7.4.3 Assembling the adapter

Refitting the adapter inlet/outlet

ActionStep

Refit the plastic spacer and O-ring (if used) on the inlet/outlet. The plastic spacer is used on the 300, 400, 450 and 800 columns, but not on the 600 and 1000 columns. Arrows in the illustration below indicate the plastic spacer and O-ring.

Guide pin

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7.4.4 Assembling the bottom assembly ActionStep

Fit a new bed support on the distributor. The smoother side of the bed sup- port shall face the interior of the column.

Refit the O-ring on the bed support screw, then wet the O-ring and the bed support screw with 20% ethanol.

Refit the bed support screw. Carefully start to tighten the bed support screw by hand making sure that the threads match. Finally tighten the bed support screw until it reaches a stop. Make sure the bed support is secured by the bed support screw.

NOTICE Be careful not to damage the threads when refitting the bed support screw.

Refit the O-ring around the outer edge of the bed support.4

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7.4.4 Assembling the bottom assembly

8 Maintenance of 1200-1600 columns

About this chapter This chapter provides instructions that are specific for performing maintenance proce- dures on AxiChrom 1200-1600 column sizes.

General maintenance procedures that are performed in the same way for all column sizes are described in Chapter 6 General maintenance and service, on page 106.

In this chapter

See pageSection

1998.1 Maintenance tools for 1200-1600 columns

2078.2 Rinse nozzle maintenance

2158.3 Lifting and lowering the bottom backing plate

2278.4 Maintaining columns with stainless steel bed supports

2688.5 Maintaining columns with plastic bed supports

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8 Maintenance of 1200-1600 columns

8.1 Maintenance tools for 1200-1600 columns

Introduction This section describes tools that are used specifically when performing service and maintenance on AxiChrom 1200-1600 columns. General tools used with all column sizes are described in Section 6.2 General maintenance tools, on page 111.

Bed support tool The bed support tool is used for removing and replacing the bed support screw. Engage the pins on the tool firmly in the screw when using the tool.

The bed support tool for 1200-1600 columns is shown below.

The threaded end of the tool is used with stainless steel bed supports to assist in centering the bed support on the distributor during assembly.

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8 Maintenance of 1200-1600 columns 8.1 Maintenance tools for 1200-1600 columns

Bed support nut tool The bed support nut tool is used for tightening the bed support nuts during assembly and disassembly of the plastic bed supports on 1200-1600 columns.

Bed support lifting device The bed support lifting device is used for the disassembly and assembly of AxiChrom 1200-1600 columns.

The bed support lifting device is used for example during:

• removal/fitting of plastic and stainless steel adapter bed supports, and the adapter distributor plate,

• removal/fitting of stainless steel bottom bed supports, and the bottom distributor plate.

The maximum lifting capacity of the bed support lifting device is 120 kg.

If a bed support lifting device is not used when performing the disassembly and assembly of AxiChrom 1200-1600 columns, it is recommended that a minimumof five people are on hand towork together to performmanual lifting operations. As it is strongly recommended that a lifting device is used to per- form maintenance steps, manual lifting operations are not described.

Note:

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Illustration of the lifting device

NoteDescriptionPart

The lifting device has three, evenly spaced, extendable arms.

Tri-arm (3×)1

Each extendable tri-arm has length ad- justment locking holes for the length ad- justment screw (1200, 1400 and 1600 extendable positions).

Tri-arm length adjustment screw (one on each arm)

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NoteDescriptionPart

The upper and lower clamps are screwed onto the threaded attachment at the end of each tri-arm as required, according to the procedure performed.

Upper (a) and lower (b) remov- able clamps1

The counter nuts fix the threaded screw in place on the tri-arm fittings. The counter nuts are loosened to adjust the threaded screw upwards or downwards and when using the threaded screws to lift stainless steel bottom bed supports and corresponding distributor plates.

Counter nuts (a) on the tri-arm threaded screw attachment (b)

Note:

The image shows the threaded screw attachment with the lower clamp fitted.

Used for columns with plastic bed sup- ports and distributors, and for the distrib- utor of columns with stainless steel bed supports.

Tri-arm center fitting - type 15

Used only with stainless steel bed sup- ports.

Tri-arm center fitting - type 26

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NoteDescriptionPart

Used with tri-arm center fitting type 2 to securely fixing stainless steel bed sup- ports to the bed support lifting device before moving the lifting device away from the column or rotating the tri-arms.

Center fitting lock and pin7

The stabilizing screws are tightened to fix the individual tri-arms in place and loosened to make minor adjustments to the positioning of the tri-arms.

Stabilizing screws (3×)8

Used to rotate the angle of the tri-arms.Side winch9

Used to raise and lower the tri-arms.Rear winch10

Used to make minor position adjustments to level the tri-arms.

Jacks on back wheels (2×)11

1 The threaded screw attachment is used in different ways to perform different lifting and moving functions. For example:

• with no upper or lower clamps fitted, to lift stainless steel bottom bed supports.

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8 Maintenance of 1200-1600 columns 8.1 Maintenance tools for 1200-1600 columns

• with the lower clamp fitted, to support stainless steel and plastic adapter bed supports.

• with both the upper and lower clamps fitted to secure bed supports and distributor plates to the tri-arms during movement of the lifting device and rotation of the tri-arms.

For further information about the bed support lifting device, refer to the third party user documentation provided by the manufacturer.

WARNING Follow the instructions provided by the manufacturer and all addi- tional user documentation safety instructions when using the lifting device.

WARNING Make sure the lifting device is in perfect working order before use. If any damage is suspected, do not use the lifting device.

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Bed support protection

A bed support protection mat and disposable protective is provided with the column.

These are also recommended accessories to keep on site for use during the maintenance of AxiChrom 1200-1600 columns to protect the bottom bed support.

The bed protection consists of two layers:

• a thin disposable plastic film (sold in sets of five) that is placed directly onto the bed support

• a thicker reusable mat that is placed on top of the plastic film.

The mats can be ordered from GE for the appropriate column size.

Nozzle O-ring assembly tool The replacement of rinse nozzle tip O-rings is performed by manually removing the O- rings from the respective nozzle tip grooves, cleaning the grooves and fitting new O- rings.

A conical nozzle O-ring expansion tool is available for expanding and fitting the new O- rings into place on the nozzle tip during rinse nozzle assembly, see Assembling the rinse nozzle, on page 212.

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8 Maintenance of 1200-1600 columns 8.1 Maintenance tools for 1200-1600 columns

Valve leakage test units Valve leakage test units are an optional tool available for AxiChrom 1200-1600 columns. The valve test units enable the media valve and rinse nozzle of the larger columns to be tested for leakage after maintenance assembly procedures have been performed, but before column reassembly is initiated.

For more information about valve leakage test units, contact your local GE representative.

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8.2 Rinse nozzle maintenance

Introduction Rinse nozzle maintenance is performed in connection with bed support maintenance.

This section describes how to disassemble and assemble the rinse nozzle so that the O- rings of the nozzle can be inspected, cleaned or changed.

The instructions in this section apply only for 1200-1600 columns. For the corresponding information for 300-1000 columns, see Section 7.2Maintaining the adapter inlet/outlet, on page 163.

Note:

Part annotations in the descriptive illustrations and tables describing the rinse nozzle in this document are not intended to represent the part number desig- nations used when ordering spare parts.

Note:

Refer to the appropriate column documentation, such as the Material Confor- mity and Spare Parts list when ordering replacement parts.

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8 Maintenance of 1200-1600 columns 8.2 Rinse nozzle maintenance

Rinse nozzle parts

9 22

13 14 15

16 17

18 19 20

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8 Maintenance of 1200-1600 columns 8.2 Rinse nozzle maintenance

DescriptionPartDescriptionPart

Decorative plugs13Inner bolts (4×)1

O-ring holder14Pivot base plate and pivot arm2

Rinse nozzle air inlets15Valve body O-rings3, 6, 8, 17

Top rinse inlet16Top mobile phase connector with seals4

Valve outer body18Top mobile phase inlet/outlet5

Support plate19Diagonally placed O-ring7

Valve inner body20Nozzle tip with O-rings and inner pipe seal

Nozzle tube holder21Nozzle inlet10

Top inlet spacer222Height adjustment bolt11

Top inlet spacer O-ring323Cylinder bolts (2×)12

2 Used only with AxiChrom 1200 column rinse nozzles. 3 When the top inlet spacer is used (AxiChrom 1200 columns) O-ring (6) is fitted between the

rinse nozzle body and the top inlet spacer, and a second O-ring (23), which is the same type and size as O-ring (6), is fitted between the top inlet spacer and the column.

Adjust nozzle tube holder The nozzle tube holder (21) is used for adjusting the position of the nozzle tip in the column, according to whether plastic bed supports or stainless steel bed supports are used. Ad- justment of the position of the tube on the rinse nozzle is necessary when switching between bed support types, as the distributor plates used with plastic bed supports are different in depth to the distributor plates used with stainless steel bed supports.

The following illustration shows the nozzle tube holder in the correct position for use with columns fitted with plastic bed supports (A) and the correct position for use with columns fitted with stainless steel bed supports (B).

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8 Maintenance of 1200-1600 columns 8.2 Rinse nozzle maintenance

To switch between nozzle tube positions the tube holder is simply unscrewed and rotated 180 degrees. The following image shows the lower tube position (A), used when columns are fitted with plastic bed supports. The arrow indicates how the position of the nozzle tube holder must be rotated through 180 degrees to position (B) to use the nozzle in columns with stainless steel bed supports.

Disassembling the rinse nozzle The numerical designations in the following instructions refer to the schematic diagram in Rinse nozzle parts, on page 208.

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ActionStep

Remove the rinse nozzle from the column as described in Remove the rinse nozzle, on page 238, or Remove the rinse nozzle, on page 274, respectively.

Remove the top rinse inlet (16) from the rinse nozzle, and then remove the O-ring from underneath it (17). Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Unscrew and remove the nozzle tip (9) from the nozzle tube, and remove the inner pipe seal and the O-ring from the end of the nozzle tube.

Remove the three O-rings from the outside of the nozzle tip by carefully making an incision in each O-ring with a small sharp blade, such as a scalpel.

Gently ease each O-ring away from the nozzle tip using blunt-ended forceps or the O-ring removal tool.

NOTICE Take great care not to damage the nozzle tip when removing the O-rings.

Unscrew the height adjustment bolt (11).

Tip:

Note the position of the adjustment bolt to make reassembly easier.

Unscrew the inner bolts (1) of the nozzle in gradual increments. Pull out the pivot base and nozzle tube from the valve outer body (18). Make sure that the O-ring holder (14) and O-ring (3) are also pulled out.

Remove the gasket, the O-ring holder (14) and the O-ring (3) from the rinse nozzle inlet (10). Clean the O-rings with 20% ethanol or replace them with new O-rings, if necessary.

Remove the cylinder bolts (12) and remove the pneumatic cylinder.8

Refit the inner bolts (1) into the valve inner body. Unscrew the refitted bolts by an even number of turns, so that all of the bolt heads protrude from the inner body by an even height.

Turn the outer body around and push the four inner bolts against a flat surface, so that the bolts are used to push out the valve inner body (20). Unscrew the bolts completely and remove the valve inner body.

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8 Maintenance of 1200-1600 columns 8.2 Rinse nozzle maintenance

ActionStep

Remove the five O-rings from the valve inner body and from underneath the valve outer body (6, 7 and 8). Clean the O-rings with 20% ethanol or re- place them with new O-rings if necessary.

Assembling the rinse nozzle

NOTICE Make sure that all O-rings and sealing surfaces are thoroughly lu- bricated with 20% ethanol before assembly.

ActionStep

Refit the diagonally placed O-ring (7) onto the valve inner body (20).

Note:

Stretch the O-ring when refitting it. Do not roll it into place.

Spray the valve inner body and O-rings thoroughly with 20% ethanol. Place the valve inner body (20) into the valve outer body (18). Make sure that the inner bolt holes (1) and top rinse inlet (16) are aligned with their corresponding positions.

Place the valve onto a clean, protective surface, and then press the valve inner body completely into the valve outer body.

Fit the valve body O-ring (3), and the O-ring holder (14) onto the nozzle inlet (10).

Refit the cylinder to the valve outer body. Tighten the bolts to a torque of 1.7 Nm.

Carefully insert the complete pivot base plate-arm assembly, including the nozzle inlet, into the valve outer body and through the valve inner body and press into place.

NOTICE Make sure that the O-ring holder and O-ring are fitted on the nozzle inlet before fitting the nozzle inlet into the valve body.

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ActionStep

Fit the inner bolts (1) and tighten the four bolts crosswise to a torque of 2.2 Nm. Refit the height adjustment bolt (11) to the same position it was in before disassembly.

Note:

While tightening the bolts, make sure that the diagonally placed O-ring (7) is not visible through the mobile phase, is thoroughly wetted, and can pass freely into the outer valve body. See section Leakage, on page 306 for more information about fitting this O-ring correctly inside the valve inner body.

Lubricate the new nozzle tip O-rings with 20% ethanol.

Tip:

Placing the O-rings intowarm, particle-freewaterwill make the O-ringsmore flexible.

Fit each nozzle O-ring onto the conical nozzle O-ring expansion tool and slide the O-ring gently down the tool to expand the O-ring to the required diameter.

Carefully align the wider end of the nozzle O-ring expansion tool with the nozzle tip (9), and then gently ease the O-rings over onto the nozzle tip and into the respective nozzle tip O-ring groove. Make sure that each O-ring sits evenly around its groove and that the O-rings are not warped, damaged or unevenly stretched.

Note:

The warmed and expanded O-rings will sit loosely in the respective O-ring groove. Place the nozzle tip with the newly fitted O-rings into hot water and the O-rings will contract to form a snug seal around the nozzle tip.

Lubricate the nozzle tube O-ring and inner pipe seal with 20% ethanol and then fit the O-ring onto the end of the nozzle tube, insert the inner pipe seal, and screw the nozzle tip onto the rinse nozzle inlet threads.

Refit the O-rings (6 and 8) into the valve outer body (18) and valve inner body (20), respectively.

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8 Maintenance of 1200-1600 columns 8.2 Rinse nozzle maintenance

ActionStep

Lubricate the top rinse inlet O-ring (17) and the valve outer body with 20% ethanol, fit the O-ring (17) under the top rinse inlet (16), and screw the top rinse inlet into the valve outer body (18). Tighten until the inlet reaches the bottom.

Lubricate the top mobile phase and corresponding O-ring and fit these to the rinse nozzle. Tighten firmly.

Test the reassembled rinse nozzle for leakage before fitting the rinse nozzle into the column, see Valve leakage test units, on page 206.

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8 Maintenance of 1200-1600 columns 8.2 Rinse nozzle maintenance

8.3 Lifting and lowering the bottom backing plate

Introduction Media valve maintenance is performed in connection with bed support maintenance (see Section 8.4 Maintaining columns with stainless steel bed supports, on page227 and Section 8.5 Maintaining columns with plastic bed supports, on page 268 ). Make sure the column is in maintenance mode before performing service operations. Follow the instruc- tions inColumndisassemblywizard, on page118 to set the column in maintenance mode and perform column disassembly before lifting the bottom backing plate. Follow the in- structions in Section 6.5Media valvemaintenance, on page142when disassembling and assembling of the media valve.

To perform some of the steps involved inmedia valvemaintenance it is neces- sary for two people to work together.

Note:

Lifting the bottom backing plate To be able to access the media valve when working with the 1200, 1400, or 1600 column it is necessary to lift the bottom backing plate. The Media valve access wizard guides the user through this procedure.

WARNING Follow all of the user documentation safety instructions and the instructions provided by the AxiChrom Master when performing maintenance on the column.

Follow the step-by-step instructions provided by the Media valve access wizard and clickConfirmafter each step. The major steps in the wizard instruction are shown below.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Recommended: If the media valve is not already in the Open position, set the media valve to Open using the Manual Control wizard in AxiChrom 300-1600 Master.

Make sure that all hoses have been disconnected to the:

• slurry inlet,

• bottom mobile phase inlet/outlet,

• rinse inlet, and

• pneumatic inlets.

NOTICE If the column is equipped with a bypass valve, make sure that the position of the bypass valve will not re- strict the movement of the bottom backing plate.

Make sure that the column disassembly wizard steps have been followed and that the column tube is locked in the swing out position before beginning to follow the Media valve access wizard procedures. In the Maintenance menu selectMedia valve access and then selectDisassembly. The adapter will move down in the next step.

Press Confirm.

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ActionStep

Carefully position the four lifting blocks over the guide pins, as shown in the image.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Insert the bottom flange bolts and washers that were removed before swing out of the column tube, as shown in the image. Fit also the appropriate dis- tance spacers under the heads of the flange bolts as shown in the following table.

Stainless steel tubeAcrylic tubeColumn size

SpacerSpacer1200

SpacerNo spacer1400

SpacerSpacer1600

When the bolts and spacers, if applicable, are in place, press Confirm.

NOTICE When bottom flange bolt distance spacers are required they are provided with the column. The function of the spacers is to prevent the bottom flange bolts from coming into contact with the column stand when lifting and lowering the bottom backing plate. The spacers are fitted under the heads of the bottom flange bolts when the bolts are used to attach the lifting blocks.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Attach the washers and nuts to the bolts to fasten the lifting blocks to the bottom backing plate. Finger tighten the bolts, then tighten all four lifting block bolts to 180 Nm.

Press Confirm.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Remove the three nuts shown in the image from the bottom of the column stand.

NOTICE Before raising the adapter, make sure that all nuts have been removed. If a nut is forgotten the column and related parts can be damaged.

Press Confirm.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

The adapter is automatically raised, hoisting the bottom plate. Make sure there are no obstacles in the way of the bottom plate.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Screw the three safety rods onto the three threaded pins in the positions shown in the image. Make sure that the safety rods are fully screwed onto the threaded pins.

The adapter will move down in the next step. Make sure there are no obsta- cles or body parts between the safety rods and the column stand.

Press Confirm.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Adjust each safety rod position by screwing the rods down until each rod sits firmly into its socket in the column stand.

Press Confirm.

Continue to Disassembling the media valve, on page 142 and subsequently Assembling the media valve, on page 148 to do the maintenance work.

Lowering the bottom backing plate

After media valve maintenance has been completed, lower the bottom backing plate according to the following instructions.

WARNING Follow all of the user documentation safety instructions and the instructions provided by the AxiChrom Master when performing maintenance on the column.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

In theMaintenancemenu selectMedia valve accessand then selectAssem- bly. Make sure that all of the lifting blocks are firmly in place, and then press Continue.

Result: The adapter will move up in the next step.

Unscrew and remove the three safety rods shown in the image, and then wrap PTFE tape around the threads of each of the three pins.

Press Confirm.

Make sure that there are no obstacles near the column that could prevent the threaded pins from entering the corresponding holes in the bottom of the column stand.

Press Confirm. The adapter will move down in the next step.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Attach the three fastening nuts and tighten to 80 Nm.4

Press Confirm.

Carefully loosen and remove the nuts and washers from the lifting block bolts.

Press Confirm.

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8 Maintenance of 1200-1600 columns 8.3 Lifting and lowering the bottom backing plate

ActionStep

Carefully remove the bolts and washers from each of the four lifting blocks.6

Press Confirm.

NOTICE Remove also the bottom flange bolt distance spacers. This does not apply for AxiChrom 1400 columns with acrylic tubes, as no distance spacers are used with these columns.

Carefully remove all four lifting blocks. The adapter will move up in the next step.

Press Confirm.

The adapter is automatically raised to the top position. Media valve Access - Assembly is completed.

Press Continue to return to the Maintenance menu.

The column is now ready for column assembly. See the Column assembly wizard, on page 128 for more information.

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8.4 Maintaining columns with stainless steel bed supports

Introduction This section describes recommended procedures for performing maintenance for columns with stainless steel bed supports.

For the maintenance schedule recommendations, see Section 6.1.3 Maintenance schedules, on page 110.

In this section

See pageSection

2308.4.1 Disassembling the bottom assembly

2388.4.2 Disassembling the adapter

2518.4.3 Assembling the adapter

2628.4.4 Assembling the bottom assembly

Precautions

CAUTION Take care not to trap fingers or other body parts when working with the bed support lifting device.

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8 Maintenance of 1200-1600 columns 8.4 Maintaining columns with stainless steel bed supports

NOTICE Do not exceed stated torque settings. Overtightening may cause damage.

NOTICE Handle the bed supports with care. The bed supports are fragile.

Always use protective gloves when handling the bed supports to avoid contaminating the bed supports.

NOTICE It is recommended to use the bed support protection when disas- sembling and assembling the adapter assembly. The bed support protection protects the bottom assembly from damage and allows the user to lay on the bottom assembly when working with the adapter assembly.

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8 Maintenance of 1200-1600 columns 8.4 Maintaining columns with stainless steel bed supports

NOTICE It is recommended to wrap PTFE tape around the threads of all bolts and screws of size M8 and above, with the exception of bolts that go into a threaded insert.

NOTICE Damage to the equipment may occur if the steps in the mainte- nance instructions are not followed.

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8.4.1 Disassembling the bottom assembly

Removing the bed support

ActionStep

Make sure the media valve is open. The media valve is open when the piston in the center of the bed support is lowered. If the media valve is closed, it can be opened using theMANUALCONTROLwizard in the AxiChrom Master.

Use the bed support tool to remove the bed support screw in the center.2

NOTICE Be careful not to slip with the bed support tool since this may damage the bed support screw and/or the bed support.

NOTICE Be careful not to damage the threads when removing the bed support screw.

Remove the O-ring on the bed support screw.3

Clean the O-ring with 20% ethanol or replace with a new O-ring, if necessary.

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ActionStep

Remove the large O-ring from the outer edge of the bed support. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Remove the small O-ring from center of the bed support.

Note:

There are two small O-rings in the center of the bed support, located one on each side of the bed support. The lower O-ring (underneath the center of the bed support) must be removed after the bed support has been lifted off from the distributor plate.

Unscrew the bed support fastening nuts from around the circumference of the column, underneath the bottom backing plate.

Make sure that the bed support lifting device is equipped with the bed support fitting (see Illustration of the lifting device, on page 201, item 6) and the threaded screws. If this is not the case, remove the clamps and install the threaded screw fittings as shown in the illustration, and screw down the counter nuts to hold the threaded screw fittings in place. Refer toBed support lifting device, on page 200.

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8.4.1 Disassembling the bottom assembly

ActionStep

Loosen the stabilizing screws in the center of the tri-arms of the bed support lifting device. This will make it easier to correctly fasten the tri-arms to the bed support. Refer to Bed support lifting device, on page200 for more infor- mation.

Wheel the bed support lifting device into position, a few centimeters above the bed support.

Note:

To correctly align the tri-arm screw fittings of the bed support lifting device with the corresponding threaded holes in the bed support or distributor plate of the bottom columnassembly, the bed support lifting devicemust bemoved into position from the side of the column as shown in the following illustration.

Align the center of the tri-arm with the center of the bottom bed support.10

Check that the tri-arm threaded screws are aligned with their corresponding threaded holes (M10) in the bed support, but make sure that the tri-arm screws are retracted so that it is the center fitting that first comes into con- tact with the bed support when the bed support lifting device is lowered.

Lower the bed support lifting device tri-arm, using the rear winch on the lifting device, until the bed support fitting on the lifting device enters and rests against the center of the bed support and the tri-arms are levelly posi- tioned above the bed support.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the arms of the lifting device.

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ActionStep

Loosen the counter nuts of the tri-arm threaded screws.13

Make sure that the tri-arms are still levelly positioned above the bed support, and then screw the tri-arm threaded screws firmly into the corresponding threaded holes in the bed support.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the arms of the lifting device.

Tighten the counter nuts on each of the tri-arm threaded screws, and then tighten the stabilizing screws (illustrated below) at the center of the tri-arms.

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8.4.1 Disassembling the bottom assembly

ActionStep

Use the rear winch on the lifting device to winch up the tri-arms and lift the bed support until it is fully clear of the bottom distributor, and then fit the center lock (see Illustration of the lifting device, on page201) before carefully moving the bed support lifting device away from the column.

NOTICE Make sure that the bed support is raised far enough to prevent any possibility of the bed support fasteners being able to scrape the polypropylene surface of the distributor.

NOTICE Take care not to bump the bed support into the column stand.

Remove the lower O-ring in the center of the bed support. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Move the bed support to desired position.18

Remove the O-ring from the distributor plate. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

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Removing the distributor plate Before removing the distributor plate the bed supportmust already have been removed separately.

Note:

ActionStep

Make sure that the bottom bed support has been removed, and then lift the bottom backing plate and disassemble and remove the media valve as de- scribed in Lifting the bottom backing plate, on page 215 and Disassembling the media valve, on page 142.

Unscrew all of the remaining M12 bolts holding the distributor to the bottom backing plate, including the four bolts located under the position previously occupied by the media valve.

Lower the bottom backing plate as described in Lowering the bottombacking plate, on page 223.

Wet the distributor plate fitting with 20% ethanol and then screw the distrib- utor plate fitting into the distributor plate center, tightening slowly. Refer to Bed support lifting device, on page 200.

Make sure that the bed support lifting device is equipped with the threaded screws.

Loosen the stabilizing screws in the center of the tri-arms of the bed support lifting device. This will make it easier to correctly fasten the tri-arms to the distributor plate.

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8.4.1 Disassembling the bottom assembly

ActionStep

Move the bed support lifting device into position over the distributor, with the center of the lifting device aligned with the center of the distributor and the tri-arms a few centimeters above the distributor plate.

Note:

Align the three threaded screws on the bed support lifting device tri-arm with the corresponding three threaded holes (M24) in the distributor.

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ActionStep

Lower the bed support lifting device tri-arm, using the rear winch. Make sure that the distributor fitting is aligned to the tri-arm adapter mounted in the center. Stop winching when the distributor fitting has clicked into place on the tri-arm adapter.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

Loosen the counter nuts of the tri-arm screws. Refer to Bed support lifting device, on page 200.

Insert the threaded screws into the matching threaded holes on the distrib- utor and fasten firmly.

Make sure that the tri-arms are levelly positioned above the distributor.12

Tighten the counter nuts on each of the tri-arm threaded screws, and then tighten the stabilizing screws (illustrated below) at the center of the tri-arms.

Lift the distributor, making sure that it is well clear of the bottom backing plate, and then slowly move the bed support lifting device away from the column.

If applicable, clean the distributor plate or change distributor plate type.15

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8.4.1 Disassembling the bottom assembly

8.4.2 Disassembling the adapter

NOTICE During all maintenance on the adapter and rinse nozzle make sure that the bottom bed support and the bottom distributor are pro- tected, for example by covering them with a plastic film and a bed protection mat.

Remove the rinse nozzle If there is little clearance space available to maneuver the rinse nozzle, pres- surized air can be used to blow the nozzle down into its open (lowest) position.

Tip:

ActionStep

Unscrew and remove the four outer nozzle bolts.1

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ActionStep

Carefully lift the rinse nozzle (and spacer, if present) up and out from the column, tilting the nozzle as required for clearance.

Tip:

Themobile phase inlet/outlet connection tube can be removed from the rinse nozzle before removing the nozzle from the column. This will provide more clearance space above the nozzle.

If necessary, disassemble the rinse nozzle and clean the O-rings in accor- dance with the instructions in Section 8.2 Rinse nozzle maintenance, on page 207.

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8.4.2 Disassembling the adapter

Remove the bed support

ActionStep

Use the bed support tool to remove the bed support screw from the center of the bed support. Hold the bed support tool vertically and firmly pressed up against the bed support screw when removing the bed support screw.

NOTICE Be careful not to slip with the bed support tool since this may damage the bed support screw and/or the bed support.

NOTICE Be careful not to damage the threads when removing the bed support screw.

Remove the O-ring in the center of the bottom side of the bed support.2

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ActionStep

Make sure that the bed support lifting device is equipped with the center fitting for stainless steel bed supports and the tri-arm lower clamps. Other- wise install the clamps and the bed support fitting. (See Section 8.1 Mainte- nance tools for 1200-1600 columns, on page199 for more information about the clamps and bed support fittings.)

Loosen the stabilizing screws in the center of the tri-arms of the bed support lifting device. This will make it easier to correctly fasten the tri-arms to the bed support.

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8.4.2 Disassembling the adapter

ActionStep

Move the bed support lifting device into position below the bed support so that the middle of the tri-arm is directly under the middle of the bed support and the tri-arm ends are under the outer rim of the bed support.

Note:

The bed support lifting device must be moved into position from the side of the column, as shown in the illustration.

Use the rear winch to gently lift the tri-arm towards the adapter. Stop winching when the bed support rests on the center fitting.

Note:

Make sure that the bed support fitting in themiddle of the tri-arm fits into the center of the bed support andmakes contact with the bed support before the tri-arm clamps. Retract the clamps a few turns if necessary.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

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ActionStep

Adjust the lower clamps up against the bed support, so that the bed support is resting securely and evenly on the three clamps of the lifting device. Level the tri-arm against the bed support.

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8.4.2 Disassembling the adapter

ActionStep

Remove the bed support fastening nuts.8

Note:

The number of fastening nuts may vary from those shown in the illustration, depending on column size. The bed support fastening nuts should release from the fasteners, leaving the fasteners attached to the distributor plate. If any fastener releases from the bed support ring instead, refit the fastener and tighten to a torque of 20 Nm.

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ActionStep

Tighten the stabilizing screws in the center of the tri-arms on the bed support lifting device, and then slowly lower the tri-arm, making sure that the bed support separates from the distributor all the way around the circumference of the column.

Lower the bed support lifting device until the bed support is fully clear of the distributor.

NOTICE Make sure that the bed support is lowered far enough to prevent any possibility of the bed support fasteners being able to scrape the polypropylene surface of the distributor.

Carefully move the bed support lifting device away from the column, taking care not to bump the bed support into the column stand.

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8.4.2 Disassembling the adapter

ActionStep

Remove the outer O-ring from the bed support. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Remove the scraper seal and its underlying O-ring from the bed support.13

Clean the scraper seal and O-rings with 20% ethanol or replace them with new seal and O-rings if necessary.

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ActionStep

Remove the O-ring in the center of the top side of the bed support. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Fit the center lock to the fitting in the center of the tri-arms if the bed support lifting device is to be used to maneuver the bed support away from the area of the column, so that the bed support can be removed from the lifting device.

Remove the O-ring from the distributor plate. Clean the O-ring with 20% ethanol or replace it with a new O-ring if necessary.

Remove the distributor plate

ActionStep

Remove the four flushing connectors according to the instruction in Sec- tion 6.7 Changing flushing connector O-rings, on page158. Clean their O-rings with 20% ethanol or replace them with new O-rings if necessary.

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8.4.2 Disassembling the adapter

ActionStep

Remove the four bolts underneath the rinse nozzle.2

Fasten the distributor plate fitting into the distributor plate center.3

Make sure that the bed support lifting device is equipped with the lower clamps. Otherwise install the clamps in accordance with the image above.

Note:

For description and specifications for the bed support lifting device see Bed support lifting device, on page 200.

Loosen the stabilizing screws in the center of the tri-arms of the bed support lifting device. This will make it easier to correctly fasten the tri-arms to the distributor plate.

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ActionStep

Move the bed support lifting device into position below the distributor plate so that the middle of the tri-arm is directly under the middle of the distributor plate and the tri-arm ends are under the outer rim of the distributor plate.

Use the rear winch to gently lift the tri-arm towards the adapter. Make sure that the distributor fitting is aligned with the tri-arm adapter. Stop winching when the distributor fitting spring-loaded pin has clicked into place on the tri-arm adapter and the distributor plate rests on the tri-arm.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

Adjust the tri-arm lower clamp fittings until all three clamps sit tightly against the distributor, with the tri-arms of the bed support lifting device level with the distributor plate.

Unscrew the bolts to remove the distributor plate. The number of bolts varies with column size.

Tighten the stabilizing screws in the center of the tri-arms of the bed support lifting device.

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8.4.2 Disassembling the adapter

ActionStep

Slowly lower the tri-arm a few centimeters, while making sure the distributor plate separates from the adapter.

Lower the distributor until it is clear of the column, and then remove the scraper seal and O-ring from the distributor plate.

Clean the scraper seal, the large O-ring around the distributor plate and the O-ring groove with 20% ethanol.

Screw the upper clamps onto the tri-arm fittings, to clamp the distributor plate securely in place on the bed support lifting device, and then pull out the bed support lifting device slowly, taking care not to bump the distributor plate into the column stand.

Move the lifting device away from the column, and then rotate the tri-arms of the lifting device through 180 degrees so that the distributor plate can be carefully maneuvered onto a clean, protected surface and removed from the bed support lifting device.

If applicable, clean the distributor plate or change the distributor plate type.15

For reassembly instructions, see Section 8.4.4 Assembling the bottomassembly, on page 262.

Note:

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8.4.3 Assembling the adapter For description and specifications for the bed support lifting device see Bed support lifting device, on page 200.

Note:

Refit the distributor plate

ActionStep

Make sure that the clean distributor plate is securely fastened to the bed support lifting device using the upper tri-arm clamps and the distributor fit- ting. Rotate the tri-arm of the lifting device if required, so that the distributor plate is in the correct orientation for fitting to the adapter.

Move the bed support lifting device close to the column.2

Make sure that the lifting device is stationary, and then remove the upper tri-arm clamps to allow the distributor plate O-ring and scraper seal to be fitted without hindrance.

Wet the large O-ring and scraper seal with 20% ethanol, then refit the O- ring on the distributor plate and place the scraper seal on top of the O-ring.

Move the bed support lifting device with the distributor plate into position below the adapter backing plate, so that the middle of the tri-arm and dis- tributor plate are directly under the middle of the adapter backing plate. Take care not to bump the distributor plate into the column stand.

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8.4.3 Assembling the adapter

ActionStep

Make sure that the holes for the flushing connectors in the distributor plate are aligned with the corresponding holes in the backing plate, as illustrated below.

Use the rear winch to gently lift the tri-arm and distributor plate towards the backing plate. Make sure that the center of the distributor plate aligns with the center of the backing plate. Stop winching when the distributor plate touches the backing plate.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

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ActionStep

Refit all of the distributor plate bolts and finger tighten, then use a torque wrench to tighten the M12 bolts on the distributor plate to 15 Nm and the four bolts underneath the rinse nozzle assembly to 11 Nm.

Release the distributor plate from the tri-arms of the lifting device using the spring loaded pin on the center fitting and lower the tri-arm a few centimeters using the rear side winch. Remove the distributor fitting from the center of the distributor plate, then carefully move the bed support lifting device away from the column.

Refit the flushing connectors according to the instructions in Section 6.7 Changing flushing connector O-rings, on page 158.

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8.4.3 Assembling the adapter

Refit the bed support

ActionStep

Make sure that the bed support is securely fastened to the bed support lifting device using the upper tri-arm clamps and the center fitting lock. Rotate the tri-arm of the lifting device if required, so that the bed support is in the correct orientation for fitting to the adapter, with the fasteners facing upwards.

Move the bed support lifting device close to the column.2

Make sure that the lifting device is stationary, and then remove the upper tri-arm clamps and loosen the stabilizing screws.

Refit the center O-ring on the upper side of the bed support.

Note:

There are two central bed support O-rings, one on the upper side and one on the underside of the bed support. The second central O-ring, on the underside of the bed support, must be fitted to the bed support after the bed support is no longer attached to the lifting device.

Carefully remove any old PTFE tape from the bed support fasteners, and then wrap the threads of all of the bed support fasteners with new PTFE tape.

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ActionStep

Refit the bed support O-ring and scraper seal, and then then fit the thin outer O-ring onto the rim of the bed support. Wet the O-rings with 20% ethanol.

Tip:

To make installation easier, make sure that the thin O-ring is thoroughly wetted with 20% ethanol during assembly. The thin O-ring may pop off if it is dry during assembly.

Move the bed support lifting device with the bed support into position below the distributor plate so that the middle of the tri-arm and bed support is di- rectly under the middle of the distributor plate. Take care not to bump the bed support into the column stand.

Make sure that the bed support fasteners are aligned with the corresponding holes in the distributor plate.

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8.4.3 Assembling the adapter

ActionStep

Use the rear winch to gently lift the tri-arm and bed support towards the distributor plate. Make sure that the bed support is level in the lifting device when raised. Use the stabilizing screws to make fine adjustments to level the bed support if required.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

Make sure that the center of the bed support aligns with the center of the distributor plate. Stop winching when there is contact with the distributor O-ring and the bed support fasteners have entered the corresponding holes in the adapter.

Note:

Make sure that the thin outer O-ring does not become dislodged, deformed or trapped when winching up the bed support.

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ActionStep

Attach the plastic and stainless steel washers and fastening nuts to the threads of the bed support fasteners, with the plastic washers closest to the backing plate. Finger-tighten the nuts onto the fasteners. Make sure that there is no gap between the assembly and the backing plate all the way around the circumference of the column.

Pull out the spring loaded pin on the distributor fitting, and then lower the bed support lifting device until it is well clear of the adapter, using the rear side winch. Carefully move the bed support lifting device away from the column.

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8.4.3 Assembling the adapter

ActionStep

Wet the threads of the bed support tool, and then use the reverse end of the bed support tool to center the bed support. With the bed support tool in place, use a torque wrench to tighten all of the bed support fastening nuts crosswise, in increments to a torque of 30 Nm, then remove the bed support tool.

NOTICE Make sure that the bed support is raised evenly against the distributor as the bed support fastening nuts are tightened. Perform a final check around the entire cir- cumference of the column after the final tension has been reached to make sure that there are no gaps and that the bed support is fully in contact with the distrib- utor around the entire column.

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ActionStep

Make sure that surfaces are wetted with 20% ethanol, and then insert the bed support screw into the center of the bed support and carefully finger tighten it, making sure that the threads are not crossed. Finally tighten the bed support screw firmly using the bed support tool, making sure that the bed support is secured in place by the bed support screw.

NOTICE Be careful not to damage the bed support or the bed support screw when using the bed support tool.

Refit the rinse nozzle If there is little clearance space available to maneuver the rinse nozzle, pres- surized air can be used to blow the nozzle down into its open (lowest) position.

Tip:

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8.4.3 Assembling the adapter

ActionStep

If the nozzle has been disassembled, assemble the rinse nozzle in accordance with the instructions in Section 8.2 Rinse nozzle maintenance, on page 207.

Tip:

To improve the amount of clearance space above the rinse nozzle when refit- ting the nozzle into the column, do not fit the mobile phase inlet/outlet con- nection tube until after the nozzle has been maneuvered into position and lowered into the center of the adapter.

then...If...

lubricate the top inlet spacer and corresponding additional O-ring with 20% ethanol, and fit the top inlet spacer and O-ring onto the nozzle body before proceeding to the next step.

the column is an AxiChrom 1200 col- umn,

Carefully align the nozzle with the corresponding guide positions in the backing plate, and then carefully lower the rinse nozzle into the column.

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ActionStep

Fit washers onto the four outer nozzle bolts, and then insert the bolts into the nozzle and finger tighten.

Tighten the nozzle bolts, crosswise and in steps, using a calibrated torque wrench, to a torque of 5 Nm.

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8.4.3 Assembling the adapter

8.4.4 Assembling the bottom assembly

NOTICE Be careful not to damage the threads when refitting the bed sup- port screw.

For description and specifications for the bed support lifting device see Bed support lifting device, on page 200.

Note:

Refit the distributor plate

NOTICE Clean O-ring grooves and lubricate all O-rings thoroughly with 20% ethanol before fitting O-rings into place.

ActionStep

Make sure that the distributor plate is securely fitted to the bed support lifting device.

Note:

For details on how to fit the distributor plate to the bed support lifting device, see Removing the distributor plate, on page 235.

Move the bed support lifting device with the attached distributor plate into position above the bottom backing plate. Align the distributor plate with the steering pins in the bottom backing plate and align the middle of the tri-arm and distributor plate directly over the middle of the bottom backing plate.

NOTICE Take care not to bump the distributor plate into the column stand.

Note:

To correctly align the distributor plate of the bottom column assembly, the bed support lifting device must be moved into position from the side of the column.

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ActionStep

Use the rear winch to gently lower the tri-arm and distributor plate towards the backing plate. Make sure that the center of the distributor plate aligns with the center of the backing plate and that the bolt holes in the distributor plate are aligned with the holes in the backing plate. Stop winching when the distributor plate touches the backing plate.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

Loosen the counter nuts of the tri-arm screws and remove the threaded screws holding the distributor to the tri-arms.

Fasten the counter nuts of the tri-arm screws, and then release the central fitting by pulling the spring loaded pin on the distributor clamp fitting.

Use the rear side winch to raise the tri-arm until it is well clear of the distrib- utor, and then remove the bed support lifting device slowly and carefully.

Remove the distributor fitting from the distributor plate.7

Check to make sure that the distributor plate sits flat against the backing plate all around the column and that the bolt holes are correctly aligned.

Tip:

Insert some of the M12 bolts, spaced at even intervals, into the underside of the backing plate, and finger tighten. This will help to make sure that the backing plate and distributor plate stay aligned when the backing plate is lifted.

Lift the bottom backing plate as described in Lifting the bottombacking plate, on page 215.

Fit the M10 screws into their respective holes around the center of the backing plate, and fit the remaining M12 bottom backing plate bolts.

Tighten the M10 screws crosswise to a torque of 11 Nm and tighten the M12 bottom backing plate bolts to a torque of 15 Nm.

Assemble the media valve as described in Assembling the media valve, on page 148.

Lower the bottom backing plate as described in Lowering the bottombacking plate, on page 223.

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8.4.4 Assembling the bottom assembly

Refit the bed support

NOTICE Clean O-ring grooves and lubricate all O-rings thoroughly with 20% ethanol before fitting O-rings into place.

ActionStep

Lubricate and then refit the O-ring on the distributor plate.1

Lubricate and refit the two small O-rings into their respective O-ring groove in the center of the the bed support, then lubricate and fit the large bed support O-ring into the bed support O-ring groove. Make sure that the large O-ring is properly seated in its groove.

Note:

There are two central bed support O-rings, one on each side of the center of the bed support. Both of these O-rings cannot be inserted at the same time when the distributor plate is attached to the lifting device. The O-ring that is situated on the lifting device side of the distributor plate can be attached before or after the lifting device is used.

Attach the bed support to the bed support lifting device as described in Re- moving the bed support, on page 230.

Carefully remove any old PTFE tape from the bed support fasteners, and then wrap the threads of all of the bed support fasteners with new PTFE tape.

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ActionStep

Move the bed support lifting device into position above the distributor plate so that the middle of the tri-arm and bed support is directly over the middle of the distributor plate.

Take care not to bump the bed support into the column stand.

Note:

Make sure that the bed support fasteners are aligned with the corresponding holes in the distributor plate. Make sure that the O-ring on the distributor is wetted with 20% ethanol.

Use the rear winch to lower the bed support until it rests on the bottom dis- tributor plate. Make sure that the bed support is flat against the distributor plate and is not tilted.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

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8.4.4 Assembling the bottom assembly

ActionStep

Loosen the counter nuts of the tri-arm threaded screws, and unscrew the tri-arm threaded screw fastenings from the bed support.

Tighten the counter nuts again and make sure that the tri-arm threaded screw fittings are securely attached to the tri-arms again and are clear of the bed support.

Use the rear side winch to lift the tri-arm until it is well clear of the bed sup- port. Carefully move the bed support lifting device away from the column.

Check that the bed support is still flat against the distributor plate, then fit the washers and bed support fastening nuts to the bed support fasteners protruding from the underside of the backing plate. Finger tighten the fas- tening nuts.

Insert the threaded end of the bed support tool into the center of the backing plate and screw into place.

With the bed support tool in place, use a torque wrench to tighten all of the bed support fastening nuts crosswise, in increments to a torque of 15 Nm.

Note:

The bed support fastening nuts can loosen when the tube is fastened into place. Check torque tensions and retighten to 15 Nm as required.

Unscrew and remove the bed support tool from the center of the bed support.14

Lubricate the bed support screw O-ring and the bed support screw with 20% ethanol, and then fit the O-ring onto the bed support screw.

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ActionStep

NOTICE Be careful not to damage the bed support or the bed support screw when using the bed support tool.

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8.4.4 Assembling the bottom assembly

8.5 Maintaining columns with plastic bed supports

Introduction This section describes recommended procedures for performing maintenance for columns with plastic bed supports.

For maintenance schedule recommendations, see Section 6.2Generalmaintenance tools, on page 111.

In this section

See pageSection

2718.5.1 Disassembling the bottom assembly

2748.5.2 Disassembling the adapter

2848.5.3 Assembling the adapter

2928.5.4 Assembling the bottom assembly

Precautions

CAUTION Take care not to trap fingers or other body parts when working with the bed support lifting device.

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NOTICE Do not exceed stated torque settings. Overtightening may cause damage.

NOTICE Handle the bed supports with care. The bed supports are fragile.

Always use protective gloves when handling the bed supports to avoid contaminating the bed supports.

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NOTICE It is recommended to wrap PTFE tape around the threads of all bolts and screws of size M8 and above, with the exception of bolts that go into a threaded insert.

NOTICE Damage to the equipment may occur if the steps in the mainte- nance instructions are not followed.

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8.5.1 Disassembling the bottom assembly

Removing the bed support

ActionStep

Make sure the media valve is open. The media valve is open when the piston in the center of the bed support is lowered. If the media valve is closed, it can be opened in the MANUAL CONTROL wizard in AxiChrom Master.

Unscrew the bed support nuts (with screws underneath that sometimes also come off when the nuts are removed).

Note:

If only the nut comes off, leave the screw in place but make sure to lift the bed support carefully and straight upwards in the following step, so that it is not damaged by the screws.

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8.5.1 Disassembling the bottom assembly

ActionStep

Use the bed support tool to remove the bed support screw in the center.3

NOTICE Be careful not to slip with the bed support tool since this may damage the bed support screw and/or the bed support.

NOTICE Be careful not to damage the threads when removing the bed support screw.

Remove the bed support by hand, lifting it straight upwards and making sure that any remaining screws do not damage the bed support. If necessary, insert the O-ring removal tool between the edge of the bed support and the O-ring and gently lever the bed support out of its seating.

Remove the inner and outer O-rings from the bed support screw. Clean the O-rings and the bed support screw with 20% ethanol. Replace with new O- rings if necessary.

Remove the O-ring from the distributor plate. Clean the distributor plate, O- ring groove and O-ring with 20% ethanol. Replace with a new O-ring if nec- essary.

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Removing the distributor plate To remove the bottom distributor plate from columns with plastic bed supports, follow the same instructions described for removing the bottom distributor plate from columns with stainless steel bed supports, see Removing the distributor plate, on page 235.

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8.5.1 Disassembling the bottom assembly

8.5.2 Disassembling the adapter It is recommended that at least two peoplework togetherwhen disassembling the adapter.

Note:

Remove the rinse nozzle Remove the rinse nozzle using the same procedure as described for columns with stainless steel bed supports, see Remove the rinse nozzle, on page 238.

Remove the distributor and bed support assembly

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ActionStep

Use the bed support tool to remove the bed support screw from the center of the bed support. Hold the bed support tool vertically when removing the bed support screw.

NOTICE Be careful not to slip with the bed support tool since this may damage the bed support screw.

NOTICE Be careful not to damage the threads when removing the bed support screw.

Remove the O-ring on the bed support screw. Clean the O-ring with 20% ethanol or replace with a new O-ring if necessary.

Remove the four flushing connectors according to the instruction in Sec- tion 6.7 Changing flushing connector O-rings, on page158. Clean the O-rings of the flushing connectors with 20% ethanol. Replace with new O-rings if necessary.

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8.5.2 Disassembling the adapter

ActionStep

Remove the four bolts underneath the rinse nozzle.4

Remove the bolts on the top of the distributor plate. The number of bolts varies with columns size.

Wet the threads of the distributor plate fitting, and then fasten the distributor plate fitting through the bed support and into the distributor plate center.

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ActionStep

Make sure that the bed support lifting device tri-arms are equipped with the lower clamps. If the tri-arms are not equipped with the lower clamps, install the lower clamps as shown in the illustration.

Loosen the stabilizing screws in the center of the tri-arms of the bed support lifting device. This will make it easier to correctly fasten the tri-arms to the bed support.

Move the bed support lifting device into position below the bed support, moving the lifting device in from the side of the column, as shown in the il- lustration, so that the middle of the tri-arm is directly under the middle of the bed support and the tri-arm ends are under the outer rim of the bed support.

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8.5.2 Disassembling the adapter

ActionStep

Use the rear winch to gently lift the tri-arm towards the adapter. Make sure that the distributor fitting is aligned with the tri-arm adapter. Stop winching when the spring loaded pin of the distributor fitting has clicked into place on the tri-arm adapter and the bed support assembly rests on the tri-arm. Adjust the three clamps so that the tri-arm is leveled against the bed support and the clamps press firmly against the bed support.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

Unscrew the nuts to remove the bed support assembly.11

Note:

The number of fastening nuts may vary from those shown in the illustration, depending column size. The nuts should release from the fasteners, leaving the fasteners attached to the distributor plate. If any fastener releases from the distributor plate, refit the fastener. Always refit the fasteners after disas- sembly.

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ActionStep

Fasten the stabilizing screws at the center of the tri-arms.12

Slowly lower the tri-arm, while making sure the distributor plate separates from the adapter.

NOTICE The lifting device must be lowered far enough to make sure that the fasteners on the top of the bed support cannot scrape the polypropylene surface of the distrib- utor.

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8.5.2 Disassembling the adapter

ActionStep

Move the bed support lifting device carefully and slowly, taking care to avoid contact between the bed support lifting device and the column stand. Move the bed support lifting device only far enough to allow access to remove the scraper seals, O-rings and distributor ring.

Note:

The upper tri-arm clamps cannot be used to hold the assembly in place until the scraper seals, O-rings and distributor ring have been removed from the distributor. Do not move the lifting device any further than necessary until the upper clamps are fitted after removal of the scraper seals, O-rings and distributor ring.

Disassemble the adapter distributor and bed support

ActionStep

Remove the upper scraper seal and O-ring. Inspect the scraper seal and O- ring and clean with 20% ethanol or replace with new components if neces- sary.

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ActionStep

Use a hex key to remove the screws holding the distributor ring, and then remove the distributor ring from the distributor.

Remove the lower scraper seal and the two O-rings. Clean the seal and O- rings with 20% ethanol or replace with new components if necessary.

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8.5.2 Disassembling the adapter

ActionStep

Fasten the distributor and bed support assembly to the bed support lifting device by fitting the upper clamps onto the tri-arms, and then use the side winch of the bed support lifting device to rotate the tri-arms through 180 degrees.

NOTICE Make sure that the tri-arm clamps and center clamp are securely fastened, so that the bed support and/or distributor cannot fall when the arms of the lifting de- vice are rotated.

1 2 3

FunctionPart

Tri-arm threaded screw fitted with upper and lower clamps1

Bed support nut2

Snap ring3

Move the bed support lifting device so that the distributor assembly is posi- tioned above a clean surface, such as a stable bench top or work surface covered with suitable protective material.

Lower the tri-arm and place the bed support and distributor assembly onto the supporting surface, allowing the assembly to rest on the fasteners or on the distributor itself.

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ActionStep

Remove the upper clamps, release the center fitting spring lock, and then winch up the tri-arm and move the bed support lifting device aside.

Remove the bed support nuts.8

Remove the snap ring using the O-ring removal tool.9

Remove the central distributor plate fitting, and then carefully lift the bed support away from the distributor.

Remove the gaskets from around the studs in the pockets of the distributor. Clean and inspect the gaskets and renew these if necessary. Check that all of the studs are firmly in position in their respective pockets.

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8.5.2 Disassembling the adapter

8.5.3 Assembling the adapter At least two persons are needed when assembling the adapter.Note:

Reassemble the bed support

ActionStep

Fit the gaskets into the recessed pockets around the studs in the distributor.1

Place the new bed support on the distributor, with the recessed screw-hole side of the bed support facing upwards.

Refit the bed support nuts (replace where necessary). The bed support nuts should be inserted so that they all just enter the extrusion in the sinter. Do not tighten the nuts.

Tighten the bed support nuts to a torque of 1.8 Nm.4

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ActionStep

Refit the snap ring. Tap gently with the plastic mallet if necessary. Use the groove on the mallet head to lever the snap ring over the edge of the bed support if the fit is tight.

Note:

Check that the snap ring is correctly and evenly mounted around the whole circumference of the bed support.

The illustration shows a cross-section of a snap-ring mounted correctly and two images of a snap-ring mounted incorrectly.

FunctionPart

Snap ringA

Scraper sealB

DistributorC

Bed supportD

Fasten the distributor plate fitting into center of the bed support, and then move the bed support lifting device into position above the bed support so that the middle of the tri-arm is directly above the middle of the bed support and the tri-arm ends are above the outer rim of the bed support.

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8.5.3 Assembling the adapter

ActionStep

Attach the bed support and distributor assembly to the bed support lifting device, and refit the upper clamps to the tri-arms of the lifting device to hold the bed support and distributor assembly securely.

Turn the tri-arm 180 degrees, and adjust the height of the lifting device using the winch until the bed support and distributor are at a suitable height for moving into position under the column.

NOTICE Make sure that the tri-arm clamps and center clamp are securely fastened, so that the bed support and/or distributor cannot fall when the arms of the lifting de- vice are rotated.

Move the lifting device into position beside the column.9

Remove the upper clamps from the tri-arm fastenings.10

Wet the O-rings and lower scraper seal with 20% ethanol. With the ribbed side of the distributor facing downwards, refit the O-rings on the distributor and place the scraper seal on top of the O-ring.

Place the distributor ring on the distributor. The two steering pins ensure that the ring can only be placed in one orientation. Make sure that the thin O-ring has not moved out of position.

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ActionStep

Refit the screws and washers to secure the distributor ring adapter. Tighten the screws crosswise and in steps to a torque of 4 Nm.

Wet the O-ring and upper scraper seal with 20% ethanol. Refit the O-ring on the distributor and place the scraper seal on top of the O-ring.

Refit the bed support

ActionStep

Move the bed support lifting device into position below the adapter, so that the middle of the tri-arm and bed support is directly under the middle of the adapter plate. Take care not to bump the bed support into the column stand.

Loosen the stabilizing screws.2

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8.5.3 Assembling the adapter

ActionStep

Make sure that the bolt holes, including flushing ports, in the adapter backing plate are fully aligned with the corresponding holes in the distributor plate.

Use the rear winch to gently lift the tri-arm upwards. Make sure that the bed support is in level when raised. Use the stabilizing screws to adjust the level.

Make sure that the center of the bed support aligns with the center of the adapter backing plate. Stop winching when the fastener is well through the adapter.

Tip:

If necessary, the jacks on the back wheels of the bed support lifting device can be used to level the lifting device.

Attach the bed support fastening nuts and plastic and stainless steel washers, with the plastic washers closest to the backing plate. Finger tighten the nuts onto the fasteners. Make sure that there is no gap between the assembly and the backing plate.

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ActionStep

Refit the four bolts into the bolt positions underneath the adapter rinse nozzle and finger tighten these bolts, making sure that the distributor is correctly positioned and centered.

Pull out the spring loaded pin on the distributor fitting and lower the lifting device using the rear side winch, and then slowly remove the bed support lifting device.

Refit the bolts on top of the distributor plate. Fit both stainless steel and plastic washers on each screw, with the plastic washer closest to the distrib- utor plate. Tighten the bolts to 15 Nm.

The following image illustrates the bolts holding the distributor plate. The number of bolts will vary depending on column size. A: Stainless steel washer. B: Plastic washer.

A B

Tighten all of the bed support fastening nuts, crosswise, to a torque of 6 Nm.9

Remove the distributor fitting from the distributor plate.10

Tighten the four central bolts, the bolt positions underneath the adapter rinse nozzle, to a torque of 11 Nm.

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8 Maintenance of 1200-1600 columns 8.5 Maintaining columns with plastic bed supports

8.5.3 Assembling the adapter

ActionStep

Refit the four flushing connectors according to the assembly instructions in Section 6.7 Changing flushing connector O-rings, on page 158.

Refit the O-ring on the bed support screw, then wet the O-ring and screw with 20% ethanol.

Spray the surfaces at the center of the bed support with 20% ethanol, then refit the bed support screw into the center of the bed support. Carefully start to tighten the bed support screw by hand making sure that the threads are not crossed.

Tighten the bed support screw firmly using the bed support tool. Make sure the bed support is secured by the bed support screw.

NOTICE Be careful not to damage the bed support or the bed support screw when using the bed support tool.

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Refit the rinse nozzle Refit the rinse nozzle using the same procedure as described for columns with stainless steel bed supports, see Refit the rinse nozzle, on page 259.

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8.5.3 Assembling the adapter

8.5.4 Assembling the bottom assembly

NOTICE Be careful not to damage the threads when refitting the bed sup- port screw.

For description and specifications for the bed support lifting device see Bed support lifting device, on page 200.

Note:

Refit the distributor plate To refit the bottom distributor plate in columns with plastic bed supports, follow the same instructions described for refitting the bottom distributor plate in columns with stainless steel bed supports, see Refit the distributor plate, on page 262.

Refit the bed support

NOTICE Clean O-ring grooves and lubricate all O-rings thoroughly with 20% ethanol before fitting O-rings into place.

ActionStep

Fit the gaskets into the recessed pockets around the studs in the distributor.1

Fit a new bed support onto the distributor, with the recessed screw-hole side of the bed support facing upwards.

Refit the O-ring on the bed support screw, then wet the O-ring with 20% ethanol.

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ActionStep

Refit the bed support nuts (replace where necessary). The bed support nuts should be inserted so that they all just enter the extrusion in the sinter. Do not tighten the nuts.

Wet the bed support screw, and then refit the bed support screw. Carefully start to tighten the bed support screw by hand, making sure that the threads match. Finally tighten the bed support screw until it reaches a firm stop.

NOTICE Be careful not to damage the bed support or the bed support screw when using the bed support tool.

Tighten the bed support nuts to a torque of 1.8 Nm.6

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8.5.4 Assembling the bottom assembly

ActionStep

Lubricate and refit the distributor O-ring outside the bed support.

Note:

When the column tube is lowered onto this O-ring,make sure that the column tube rests evenly on the O-ring around the entire diameter of the column.

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9 Master maintenance

About this chapter This chapter provides instructions on how to update the Master firmware.

In this chapter

See pageSection

2969.1 AxiChrom 300-1600 Master firmware installation

2979.2 Updating AxiChrom 300-1600 Master firmware

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9 Master maintenance

9.1 AxiChrom 300-1600 Master firmware installation

Prerequisites The AxiChrom 300-1600 Master firmware has to be updated if the column delivered is not available in the existing firmware, or if new functionality has been implemented.

Compare the functionality and compatibility of your Master firmware with the Capability Matrix supplied in the AxiChrom 300-1600 Master firmware upgrade kit to see if the Master firmware needs to be updated.

Safety precautions

WARNING The end user must ensure that all installation, maintenance, oper- ation and inspection is carried out by qualified personnel who are adequately trained, understand and adhere to local regulations and the operating instructions, and have a thorough knowledge of the entire system and process.

WARNING High voltage inside the Master. Never open the door before the Master has been turned off, the Master power switch has been switched off, and the Master power cable has been disconnected or external electric switch has been switched off.

CAUTION Make sure that no liquid gets into the Master. Do not handle any liquid in the area around the Master when the Master door is open.

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9.2 Updating AxiChrom 300-1600 Master firmware

Install/update the Master firmware

Follow this procedure to install or update the AxiChrom 300-1600 Master firmware.

ActionStep

With the AxiChrom 300-1600 Master already powered up, insert the USB memory stick into the USB port located on the rear panel of the AxiChrom 300-1600 Master.

Result: The Master firmware update will begin automatically.

When the firmware has updated, use the Power Switch on the Master con- nector panel to turn off the power, and then remove the USB stick from the USB port.

Disconnect the Master power cord, or switch off the external electric supply switch.

Open the door of the Master cabinet.4

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9 Master maintenance 9.2 Updating AxiChrom 300-1600 Master firmware

ActionStep

Unscrew the two screws holding the plastic cover and pull off the plastic cover.

Pull the Compact Flash memory card out from its holder.6

Insert the new Compact Flash memory card.7

Put the plastic cover back in position, and replace the two screws.8

Close the door on the Master, and then reconnect the power cord.9

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9 Master maintenance 9.2 Updating AxiChrom 300-1600 Master firmware

Implement and verify firmware update

After completing the install/update of the Master firmware, the Master must be restarted to implement the firmware update and to check that the firmware has been installed correctly.

ActionStep

After completing the install/update of the Master firmware, turn on the AxiChrom 300-1600 Master using the Power Switch on the AxiChrom 300-1600 Master connector panel and login as described in Login procedure, on page 83.

Continue to the Select column dialog and then press Configuration.2

In the Configuration dialog, select System Info.3

In the System info dialog, check that:4

• the Application rev. under DriveLogix is the same as the one stated on the packing list.

• theApplication rev. underOperator panel is the same as the one stated on the packing list.

Add column information Data about previously connected columns is not stored following a software/firmware upgrade. To re-establish contact between the AxiChrom 300-1600 Master and a con- nected column after a firmware update, follow these instructions.

ActionStep

On the AxiChrom 300-1600 Master touch screen panel, press Continue and then Back to enter the Select column dialog.

In the Select column dialog, pressAdd to populate the column list with infor- mation about the connected column.

Note:

All column data must be re-entered after a software/firmware update is per- formed.

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9 Master maintenance 9.2 Updating AxiChrom 300-1600 Master firmware

10 Cleaning and storage

Introduction This chapter presents a general overview of the cleaning and storage procedures that apply to all AxiChrom column sizes.

Information about cleaning stainless steel bed supports is also provided.

In this section:

See pageSection

30110.1 Cleaning an empty column

30310.2 Cleaning stainless steel bed supports

30410.3 Storage

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10 Cleaning and storage

10.1 Cleaning an empty column

Introduction Cleaning the column using the procedure described in this section should be performed:

• before any maintenance or service work is performed on the column,

• to prepare the column for packing with chromatography media,

• after chromatography media has been unpacked from the column.

For instructions about cleaning the column when the column is new (that is, when the column is filled with transportation/storage solution), see Section 5.3 Cleaning a new column, on page 88.

Cleaning procedure Formore information about cleaningmethods, see the AxiChromUserManual.Note:

To clean the column:

ActionStep

Clean the outside the inside of the acrylic tube with for example water and/or 20% (v/v) ethanol to remove any surface residues from the packaging.

NOTICE Do not use ethanol stronger than 20% (v/v) for cleaning the acrylic tube, since this may damage the column tube.

Clean steel surfaces with a wetted cloth and and a suitable solution, such as 70% (v/v) isopropanol or 70% (v/v) ethanol.

Make sure that the adapter seals and O-rings are clean from particles and waste, as these may damage the adapter and column tube when the adapter is moving.

Perform a priming according to the instructions in the Priming wizard, in the AxiChrom MasterMainmenu. This procedure will rinse the column and its tubes and remove any air from the column. More information about Master wizards can be found in the AxiChrom User Manual.

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10 Cleaning and storage 10.1 Cleaning an empty column

ActionStep

Make sure that the cleaning solution is completely flushed out and replaced by an appropriate storage solution after priming the column.

Result: The column is now primed and cleaned.

Cleaning the Master To clean the Master:

ActionStep

Turn off the AxiChrom Master and disconnect the power cable.1

Wipe external surfaces with a damp cloth and/or 20% (v/v) ethanol.2

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10 Cleaning and storage 10.1 Cleaning an empty column

10.2 Cleaning stainless steel bed supports Stainless steel bed supports may clog if not cleaned regularly. The supports should be cleaned at least after 5 to10 packings, but preferably after each packing. If the supports cannot be cleaned properly using the method below, contact your local GE representative for advice.

The compressed air that is used for cleaning bed supportsmust be completely free from lubricating oils and particles.

Note:

ActionStep

Remove the bed support from the column.1

Flush the bed support free from entrapped media using water.2

Place a shut-off valve on a compressed air supply hose. Fit a 0.5 to 1 inch plastic TC-clamp connector or similar to the valve.

Use a shallow container to soak the bed support in 0.5 to 1 M NaCl solution for a maximum of 5 minutes.

Place the bed support with the coarse side up on a clean non-scratching (plastic) support, that allows air and media to flow through the bed support.

Open the valve and start blowing air though the bed support from a close distance. Make sure the whole surface of the bed support is properly blown and that special care is used at the outer rim and at the center.

Rinse the bed support thoroughly in water immediately after the above procedure is finished.

Repeat the procedure, this time using at least 20% ethanol solution. If 20% ethanol cannot be used, use water. Finish by carefully rinsing with water.

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10 Cleaning and storage 10.2 Cleaning stainless steel bed supports

10.3 Storage

Before storage The following procedure is recommended before long-term storage of the column:

ActionStep

Perform Cleaning-In-Place according to your recommended cleaning proce- dure (for example using 0.5 to 1.0 M sodium hydroxide for 1 to 4 hours).

If the column will be filled with 20% (v/v) ethanol as the storage solution (see step 4), the column should be rinsed with a suitable buffer that neutralizes the pH. The buffer must not contain any chlorides as these may cause cor- rosion.

Move the column adapter down to 2 cm above the bottom bed support, leaving the space between the adapter and bottom bed support completely filled with the storage solution.

Pump a suitable storage solution into the column (for example 0.01 M sodium hydroxide or 20% (v/v) ethanol solution), using an amount of storage solution corresponding to five times the present column volume.

Close all valves and fit blind caps on all inlets/outlets of the media valve.5

Disconnect all tubes connected to the top inlet. If the tubes are not discon- nected a vacuum may form in the next step.

Fit a blind cap on the top inlet.7

Storage conditions The following conditions shall be maintained while the column is in storage:

• Store the column indoors in a dust-free environment with a well-controlled climate.

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10 Cleaning and storage 10.3 Storage

• Keep air humidity and air temperature differences as low as possible to prevent condensation and corrosion.

• Maintain the storage area at a constant temperature, below 24°C if the storage so- lution used in the column is 2% benzyl alcohol or 20% ethanol.

• Make sure the relative humidity is between 0% to 95% , non-condensing for column storage and between 0% to 80% , non-condensing for storage of the AxiChrom 300-1600 Master (store preferably in low humidity conditions).

• Do not expose the column to chlorides.

• Change the storage solution regularly, at least once every three months.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 305

10 Cleaning and storage 10.3 Storage

11 Troubleshooting

About this chapter This chapter provides information to assist users and service personnel to identify and correct problems that may occur when operating AxiChrom columns.

If a problem persists or cannot be resolved using the suggested actions in this guide, contact your GE representative for advice.

Overcurrent

Suggested actionPossible causeSymptom

Power off the AxiChrom 300-1600 Master and restart to recalibrate the motor. See Section 5.2 Starting up and shutting down the AxiChrom 300-1600 Master, on page 82.

The motor is not correctly calibrated

Overcurrent

• Make sure the chromatography media is not packed too densely.

• Make sure there is no blockage in valves or tubing.

• Check that correct flow rates are used.

The motor is working against too high back- pressure.

Leakage

Suggested actionPossible causeSymptom

Make sure that flushing ports are open during priming, and are not bent or obstructed in any way.

Fluids may have been trapped between parts during priming.

Leakage at adapter bed supportfasten- ing nuts Check if there is a continuous leakage or if it stops

after the column has settled after temperature or pressure change. If the leakage stops it is likely that the leakage was consisting of fluids from priming.

306 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

11 Troubleshooting

Suggested actionPossible causeSymptom

• If the O-ring is not fitted into the O-ring groove on the inner body: remove the inner body, fit the O-ring correctly and then refit the inner body inside the outer body.

• If the O-ring is visible but still in the groove, make sure that the bolts holding the inner body are correctly tightened to a torque of 2.2 Nm. When installing the inner body in the outer body it is important to tighten the top screws crosswise and make sure that the mobile phase channel does not cause wear on the diagonal O-ring. Make sure the O-ring is thor- oughly wetted when it is installed.

The diagonal O-ring on the valve inner body is not correctly fitted. When you look through the con- nector or mobile phase (disas- sembled) the O-ring surface should not be seen (see image of O-ring not fitted correctly). Use a flashlight as the white color of the O-ring may be diffi- cult to see.

Leakage at media valve or adapter inlet/outlet

Check that piping is correctly angled and adjust piping supports if required.

Valve piping is not connected correctly.

Leakage at bottom mobile inlet

• Regularly inspect O-rings and seals. Replace if wear is detected.

• Check that O-rings are not twisted or dam- aged. Refit or replace as necessary.

• Make sure that O-rings are thoroughly wetted when installed. Replace damaged parts with new parts. If no damaged parts are found, re- installation may resolve the issue.

• Make sure there is no gap between the O-ring and the bed support or between the O-ring and the column tube.

An O-ring or seal does not form a tight sealing surface.

Continuous leakage

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 307

11 Troubleshooting

Assembly difficulties

Suggested actionSymptom

Make sure to use the centering tool to properly center the stainless steel bed support. Follow the specified instruction and make sure to tighten the bed support fastening nuts crosswise in steps.

The center screw cannot be fitted.

Pressure transmitter fault

Suggested actionPossible causeSymptom

Check that the transmitter cable is cor- rectly connected. If the problem persists contact your GE service operator.

Transmitter not connected correctly, or faulty

Pressure transmitter fault

Replace the gasket. Check the transmitter membrane for damage and replace the transmitter if necessary.

The TC gasket is too small or is stuck on the pressure transmitter membrane

Pressure transmitter shows high pressure even though the column is depressurized

Lock block damage

Suggested actionSymptom

If a lock block does not sit firmly in place the lock block may have been damaged, for example by having been exposed to excessive force. Replace the lock block.

Lock block does not remain firmly in place.

If a lock block is difficult to fit, for example if it is difficult to screw down, this indicates that the lock block is damaged. Replace the lock block.

Lock block is difficult to fit/lock block is difficult to screw down

Inspect the lock blocks after each use and replace blocks if any damage is observed.

Visual inspection shows lock block damage

308 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

11 Troubleshooting

Master issues

Suggested actionPossible causeSymptom

• Make sure that connections between the Master and column are correctly fitted and properly tightened.

• Make sure that the procedure de- scribed in Section 5.2 Starting up and shutting down the AxiChrom 300-1600 Master, on page 82 has been followed.

Loose connection, damaged cable connection between the Master and the column.

Recurring alarms that cannot be solved by reset- ting the Master.

The Master works inter- mittently.

Press the EMERGENCY STOP button and contact your GE service operator.

An AxiChrom Master fuse may have blown

Master screen shut-down

Disconnect the column from the Master. Reconnect the column to the Master and restart the Master.

Loose connection, communi- cation not established.

The Master does not rec- ognize the column.

Reset the EMERGENCY STOP by pressing the button and turning it clockwise.

The EMERGENCY STOP may have triggered during start- up.

The Master does not start.

Media valve

In rare cases the media valve may get stuck in closed position. First, check that the fol- lowing conditions are met:

• The compressed air requirements are fulfilled. The pressure should be between 5.5 and 7 bar, see Compressed air requirements, on page 79.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 309

11 Troubleshooting

• The pneumatic air or other tubes are correctly connected, see Connections on the AxiChrom 300-1600 Master, on page 68.

• The media valve is primed before operation, see Section 5.6 Priming the column, on page 96.

If the media valve cannot be opened when the above conditions have been met, the O- ring in the media valve may have stuck to the piston. This may have happened if the column has been unused for a while. Try the following:

1 Check that the compressed air and AxiChrom Master are connected to the column and the media valve mode in theManual Controlwizard is set toOpen. Even though nothing happens visually when the media valve is opened, this will release a lock in the pneumatic cylinder.

2 Place a screwdriver on top of the washer at the end of the piston of the pneumatic cylinder. Carefully lever against the bottom end of the pneumatic cylinder until the media valve opens.

3 If this does not work, contact a GE service operator.

310 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

11 Troubleshooting

12 Reference information

About this chapter This chapter provides reference information that may be useful when installing, operating, maintaining or performing troubleshooting forAxiChrom columns. Information that may be useful when ordering parts or associated equipment is also provided.

In this chapter This chapter contains the following sections:

See pageSection

31212.1 Specifications

31412.2 Weights and dimensions

32012.3 Spare parts and accessories

32112.4 Chemical resistance

32512.5 System recommendations

32612.6 Health and Safety Declaration Form

32812.7 Ordering information

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 311

12 Reference information

12.1 Specifications AxiChrom columns must always be powered and operated via a connected AxiChrom Master. All equipment is intended for indoor use only.

Note:

General technical column specifications

ValueParameter

See Column dimensions, on page 314 and Column weights, on page 315

Dimensions

See Column dimensions, on page 314 and Column weights, on page 315

Weights

• for AxiChrom 300-1000 columns: +2°C to +30°C

• for AxiChrom 1200-1600 columns: +15°C to +30°C

Operating temperature, including operating environment ambient temperature

+4°C to +30°C1Storage environment ambient temperature

0% to 95%, non-condensingRelative humidity

1000 mMaximum altitude2

1 It is recommended that the temperature of the storage environment does not exceed +24°C if the storage solution used in the column is 2% benzyl alcohol or 20% ethanol.

2 According to the corresponding electrical standards fulfilled by the product.

312 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.1 Specifications

General technical AxiChrom Master 300-1600 specifications

ValueParameter

IP 56, NEMA 4XProtection class

1Enclosure type

380-480 V AC, 50-60 Hz with protec- tive ground

Supply voltage

480 Y/277 V ACMax voltage (North America)

14.5 ALargest motor current (Full Load Ampere)

21.1 AMaximum current (Full Load Ampere)

8500 VAMax power consumption

± 10% of nominal voltageMax mains supply voltage fluctuation

Overvoltage category IITransient overvoltage level1

5.5 to 7 bar gPneumatic supply

+2°C to +30°CAmbient temperature, operating environment

+4°C to +30°CAmbient temperature, storage environment

Maximum relative humidity 80%Relative humidity

2000 mMaximum altitude2

2Pollution degree

1 The Master is designed to be able to be operated in a safe way if TEMPORARY OVERVOLTAGES occur on the MAINS supply.

2 According to the corresponding electrical standards fulfilled by the product.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 313

12 Reference information 12.1 Specifications

12.2 Weights and dimensions

Column dimensions The product documentation delivered with the column states the actual weight and dimensions of each individual column.

Typical weights and dimensions for the different column sizes are listed in the table below.

The min. and max. heights refer to the height of the column with the adapter in its lowest position for transportation, and with the adapter in its highest position, for example for maintenance work.

The illustration shows the orientations designated as length (L) and width (W) in the tables.

L W

Max. height [mm]

Min. height [mm]

Footprint (length × width) [mm]

Column bed heights [mm]

Column diameter [mm]

222014501110 × 520100 to 300300

272017101110 × 520100 to 500300

314 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.2 Weights and dimensions

Max. height [mm]

Min. height [mm]

Footprint (length × width) [mm]

Column bed heights [mm]

Column diameter [mm]

222014601110 × 600100 to 300400

272017201110 × 600100 to 500400

223014801110 × 620100 to 300450

275017401110 × 620100 to 500450

234015901180 × 780100 to 300600

286018501180 × 780100 to 500600

263018801470 × 1080100 to 300800

315021401470 × 1080100 to 500800

265018901720 × 1300100 to 3001000

317021501720 × 1300100 to 5001000

299022202020 x 1510100 to 3001200

351024802020 x 1510100 to 5001200

315023802220 x 1740100 to 3001400

367026402220 x 1740100 to 5001400

316023902320 x 1960100 to 3001600

368026502320 x 1960100 to 5001600

Column weights The table below lists the weights of empty columns and crate+column for columns with acrylic column tubes and for columns with stainless steel column tubes. All weights are given in kg.

Weights are stated for columns with plastic bed supports/stainless steel bed supports.

Crate+column with stainless steel tube

Crate+column with acrylic tube

Empty column with stainless steel tube

Empty column with acrylic tube

Column

609/615574/580449/455414/420300 short tube

634/640599/605469/475434/440300 long tube

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 315

12 Reference information 12.2 Weights and dimensions

Crate+column with stainless steel tube

Crate+column with acrylic tube

Empty column with stainless steel tube

Empty column with acrylic tube

Column

648/657611/620488/497451/460400 short tube

660/675636/645501/510471/480400 long tube

919/930879/890739/750699/710450 short tube

974/985934/945789/800749/760450 long tube

1072/10891013/1030877/894818/835600 short tube

1178/11951088/1105973/990883/900600 long tube

2689/27172472/25002339/23672122/2150800 short tube

2832/28602572/26002472/25002212/2240800 long tube

3166/32092957/30002726/27692517/25601000 short tube

3407/34503087/31302957/30002637/26801000 long tube

5850/59005410/54605322/53764884/49381200 short tube

6000/60505610/56605464/55185074/51281200 long tube

8310/83807860/79307741/78107293/73621400 short tube

8490/85608130/82007906/79757535/76041400 long tube

10 700/10 80010 200/10 30010 090/10 1689597/96751600 short tube

10 900/11 00010 500/10 60010 255/10 3339874/99521600 long tube

Weights of column parts The column weights and specifications can be found on the rating plate and in Column dimensions, on page314andColumnweights, on page315. The table below states specific part weights. See the location of the main parts in the assembly drawing (AD) provided with your column.

Note: • All weights are given in kilograms.

• A dash (-) means that the item does not exist for that col- umn.

1600140012001000800600450400300Column

.........Top unit

5700436425661390936430190164130• Short column tube

5747440026101420956460198172138• Long column tube

316 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.2 Weights and dimensions

1600140012001000800600450400300Column

.........Column tube (acrylic column tube) 916744537363242132756256 • Short column tube 1112895648433288162937569 • Long column tube

.........Column tube (stainless steel column tube) 140911929755843511781069273 • Short column tube 14931266103863739420913011389 • Long column tube

.........Adapter bed support

45383022.314.48.75.74.73.2• Stainless steel

103775532.920.910.55.84.52.4• Plastic (including distributor)

.........Adapter distributor

95725227.116.98.64.63.51.8• Stainless steel

94715129.718.99.75.34.12.2• Plastic

.........Bottom bed support

55473827.119.711.17.16.14.2• Stainless steel

9753.22.00.80.50.40.2• Plastic

.........Bottom distributor

98775843.427.813.77.96.44.5• Stainless steel

103816245.529.614.68.67.15.0• Plastic

5.85.85.82.32.31.31.31.31.3Media valve assembly

---0.90.90.50.50.50.5Top mobile phase inlet/outlet

5.75.75.7------Rinse nozzle1

---0.40.40.10.10.10.1Locking pin

0.90.90.9------• Short column tube

1.41.41.4------• Long column tube

1.61.61.6110.40.40.40.4Lock block

1 AxiChrom 1200-1600 columns only.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 317

12 Reference information 12.2 Weights and dimensions

Master weights and dimensions

ValueParameter

90 (including cables connected to the Master)Weight [kg]

670Width [mm]

630Length [mm]

1090Height [mm]

Crate weights and dimensions Typical weights and dimensions for the different crate sizes are listed in the table below.

Empty crate weight [kg]

Crate width [mm]

Crate length [mm]

Crate height [mm]

Column bed heights [mm]

Column di- ameter [mm]

16014959701920100 to 300300

16514959702180100 to 500300

16014959701920100 to 300400

16514959702180100 to 500400

180148010901920100 to 300450

185148010902180100 to 500450

195139511001875100 to 300600

205139511002135100 to 500600

350180014402190100 to 300800

360180014402450100 to 500800

440204016502240100 to 3001000

450204016502500100 to 5001000

520179023012462100 to 3001200

530179023012722100 to 5001200

570194024962582100 to 3001400

318 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.2 Weights and dimensions

Empty crate weight [kg]

Crate width [mm]

Crate length [mm]

Crate height [mm]

Column bed heights [mm]

Column di- ameter [mm]

580194024962842100 to 5001400

610224025962582100 to 3001600

630224025962842100 to 5001600

657707001300Master

Individual crateweightsmay vary according to theweights of any accessories delivered with the column.

Note:

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 319

12 Reference information 12.2 Weights and dimensions

12.3 Spare parts and accessories

Spare part list A list of spare parts can be found in the product documentation for the column. The list can also be found online at www.gelifesciences.com/axichrom.

Spare parts recommended to keep on site

GE recommends that spare parts used more often are kept on site. Plastic bed supports, snap rings, seals and O-rings are examples of spare parts that are useful to have in stock.

For information about the recommended frequency for replacement of specific compo- nents, see Section 6.1.3 Maintenance schedules, on page 110.

320 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.3 Spare parts and accessories

12.4 Chemical resistance AxiChrom columns are resistant to chemical agents used in protein recovery, including buffer solutions for adsorption, elution and washing, and to solutions effective in cleaning, sanitization and storage. The following table lists chemicals that may or may not be used with AxiChrom columns. The concentrations listed are not normally exceeded during an operating cycle.

NOTICE Chlorides and low pH can cause corrosion on stainless steel. Rinse thoroughly with clean water after use.

Inspect the column regularly for signs of corrosive attacks, which may cause column damage if untreated. Note that the stainless steel bed supports are especially vulnerable to corrosion.

The information in the table has been collected from several published sources, not from individual tests on column components. It should be used only as a guide. The effect of chemicals will generally be more severe at higher temper- atures. Note also that the combined effects of agents have not been taken into account in this table. The applicable chemical resistance depends on the con- figuration of the column and the associated materials of construction.

Note:

CAS no.3Temperature limits2

CommentsTime/cycle restrictions

Concen- tration1

Chemical

64-19-72°C to 30°CCleaning-In-Place (CIP)

3 h25%Acetic acid

67-64-12°C to 30°CEfficiency test1 h2%Acetone

7783-20-22°C to 30°CAdsorption5 h2 M 4Ammonium sulfate

100-51-62°C to 24°CStorage12 months2%5Benzyl alcohol

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 321

12 Reference information 12.4 Chemical resistance

CAS no.3Temperature limits2

CommentsTime/cycle restrictions

Concen- tration1

Chemical

64-17-52°C to 24°CStorage12 months and max. 0.5 bar

20%Ethanol

64-17-52°C to 30°CCIP3 h70%6Ethanol

64-17- 5/64-19-7

2°C to 30°CCIP3 h20%/10%Ethanol/acetic acid

50-01-12°C to 30°CCIP5 h6 M 7Guanidine hy- drochloride

7647-01-02°C to 30°CCIP1 h0.1 M (pH = 1)8

Hydrochloric acid

67-63-02°C to 30°CCIP1 h30%9Isopropanol

7664-38-22°C to 30°CFor passivation of stainless steel bed supports

8 h5%Phosphoric acid

71-23-82°C to 30°CWetting plastic bed supports

16 h20%1-Propanol

7647-14-52°C to 30°CPurification, CIP3 h0 to 3 M 10Sodium chloride

1310-73-22°C to 30°CCIP24 h, room temp. to 30°C

1 M (pH = 14)

Sodium hydroxide

1310-73-22°C to 30°CStorage12 months0.01 M (pH = 12)

Sodium hydroxide

1310-73- 2/ 64-17-5

2°C to 30°CCIP3 h1 M/ 20%Sodium hydroxide/ ethanol

7757-82-62°C to 30°CAdsorption3 h1 M4Sodium sulphate

57-13-62°C to 30°CPurification, CIP5 h8 M4Urea

322 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.4 Chemical resistance

CAS no.3Temperature limits2

CommentsTime/cycle restrictions

Concen- tration1

Chemical

2°C to 30°CEquilibration, ad- sorption, elution

24 h10 to 250 mM, pH 2 to 10

Aqueous buffers com- monly used in chromatogra- phy

1 When a concentration is given as a percentage, this is v/v. 2 Do not exceed the operating temperature specifications for the individual column size. 3 CAS no.: Registration number assigned by the Chemical Abstract Services (CAS), American Chemical Society. 4 pH in these solutions depends on the pH of the buffer, which can vary between 3 and 13. 5 2.0% v/v benzyl alcohol is compatible with the acrylic material used in AxiChrom columns at room temperature

for up to one year. 6 On stainless steel column parts only. Exposure of other column parts to ethanol at higher concentrations than

20% can damage the column. 7 Not for use with columns containing wetted components of stainless steel. 8 pH below 4 for stainless steel is not recommended. 9 Applies only to acrylic column tubes. 10 For columns containing wetted stainless steel components max 1.0 M NaCl is recommended. For additional

important information, see Section 12.4.1 Sodium chloride and strong pH solutions, on page 324.

Avoid using the following chemicals in AxiChrom columns

• Extreme oxidizers

• Fluorine and halogenated compounds

• Chlorinated solvents (such as methylene chloride)

• Esters

• Aromatic hydrocarbons (such as toluene)

• Alcohols at concentrations higher than those specified in the table above

• Salt in combination with pH below 4. Always wash the column with at least five col- umn volumes of pH-neutral solution (water) between and after use of salt and low pH buffers.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 323

12 Reference information 12.4 Chemical resistance

12.4.1 Sodium chloride and strong pH solutions Depending on configuration, AxiChrom columns can be equipped with wetted compo- nents of stainless steel and must therefore be appropriately maintained when exposed to sodium chloride (NaCl) during chromatographic processes. While the stainless steel bed supports have a high degree of resistance to corrosion, they may corrode on extend- ed exposure to NaCl. The degree of susceptibility varies according to conditions of use. Low pH in combination with NaCl, high temperature and damage to the steel surface all increase the risk of corrosion.

If a stainless steel surface is damaged (scratches, impact marks etc.) it has to be cleaned and passivated prior to being taken into operation. Your GE representative can provide guidance on suitable cleaning and passivation procedures if required.

It is recommended that a water rinse of at least five column volumes is used to remove NaCl from stainless steel surfaces. Water is the preferred rinse solution because of the solubility properties of sodium chloride in water.

Columns should be stored with solutions free from chloride ions.

324 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.4 Chemical resistance 12.4.1 Sodium chloride and strong pH solutions

12.5 System recommendations Refer to UNICORN user documentation supplied, or contact your local GE representative for the most current information.

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 325

12 Reference information 12.5 System recommendations

12.6 Health and Safety Declaration Form

On site service

On Site Service Health &

Safety Declaration Form

Service Ticket #:

To make the mutual protection and safety of GE service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, please complete this checklist and present it to the Service Engineer upon arrival. Equipment and/or work areas not sufficiently cleaned, accessible and safe for an engineer may lead to delays in servicing the equipment and could be subject to additional charges.

Yes No Please review the actions below and answer “Yes” or “No”. Provide explanation for any “No” answers in box below.

Instrument has been cleaned of hazardous substances. Please rinse tubing or piping, wipe down scanner surfaces, or otherwise ensure removal of any dangerous residue. Ensure the area around the instrument is clean. If radioactivity has been used, please perform a wipe test or other suitable survey.

Adequate space and clearance is provided to allow safe access for instrument service, repair or installation. In some cases this may require customer to move equipment from normal operating location prior to GE arrival.

Consumables, such as columns or gels, have been removed or isolated from the instrument and from any area that may impede access to the instrument .

All buffer / waste vessels are labeled. Excess containers have been removed from the area to provide access.

Provide

explanation

for any “No”

answers here:

Equipment type / Product No: Serial No:

I hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the area has been made safe and accessible.

Name: Company or institution:

Position or job title:

Date (YYYY/MM/DD):

Signed:

GE and GE monogram are trademarks of General Electric Company. GE Healthcare Bio-Sciences Corp, 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327 © 2010-14 General Electric Company—All rights reserved. First published April 2010.

DOC1149542/28-9800-26 AC 05/2014

326 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.6 Health and Safety Declaration Form

Product return or servicing

Health & Safety Declaration Form

for Product Return or Servicing

Return authorization number:

and/or Service Ticket/Request:

To make sure the mutual protection and safety of GE personnel, our customers, transportation personnel and our environment,

all equipment must be clean and free of any hazardous contaminants before shipping to GE. To avoid delays in the processing of

your equipment, please complete this checklist and include it with your return.

1. Please note that items will NOT be accepted for servicing or return without this form

2. Equipment which is not sufficiently cleaned prior to return to GE may lead to delays in servicing the equipment and

could be subject to additional charges

3. Visible contamination will be assumed hazardous and additional cleaning and decontamination charges will be applied

Yes No Please specify if the equipment has been in contact with any of the following:

Radioactivity (please specify)

Infectious or hazardous biological substances (please specify)

Other Hazardous Chemicals (please specify)

Equipment must be decontaminated prior to service / return. Please provide a telephone number where GE can contact you for additional information concerning the system / equipment.

Telephone No:

Liquid and/or gas in equipment is: Water

Ethanol

None, empty

Argon, Helium, Nitrogen

Liquid Nitrogen

Other, please specify

Equipment type / Product No:

Serial No:

I hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the area has been made safe and accessible.

Name: Company or

institution:

Position or job title: Date (YYYY/MM/DD)

Signed:

To receive a return authorization number or service number, please call local technical support or customer service.

GE and GE monogram are trademarks of General Electric Company. GE Healthcare Bio-Sciences Corp, 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, US

DOC1149544/28-9800-27 AC 05/2014

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 327

12 Reference information 12.6 Health and Safety Declaration Form

12.7 Ordering information Current lists of the spare parts available for AxiChrom columns, as well as details and information about ordering accessory components and wheel kits can be found by contacting your local GE representative or by visiting www.gelifesciences.com/axichrom.

328 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

12 Reference information 12.7 Ordering information

Index A Adapter inlet/outlet, 163 Assemble wizard, 141

B Bed support nuts (intermediate fastening screws), 293 Bed support protection mat, 205

C CE marking, 11 Cleaning

Cleaning-In-Place, 104 column, 301 Master, 302 Sanitization-In-Place, 104 stainless steel bed sup- ports, 303

Cleaning a new column, 88 Column tubes, 48 Connecting the column, 70

D Disassemble wizard, 128

E Emergency procedures

Master shutdown, 35 shutdown of a connected pumping system, 36

Evaluate column perfor- mance, 103

F FCC compliance, 11 Flushing channels, 155 Forms

Health and Safety, 109

G Grounding, 78

I Intelligent packing, 50

Intended use, 7

L Labels, 31

column label, 32 hazardous substances, 34 master label, 32 symbols, 34

Leakage test (integrity test- ing), 91

M Maintenance

bed support lifting de- vice, 200 bed support nut tool, 200 bed support tool for 300- 1000 columns, 162 bed support tool for 1200- 1600 columns, 199 cleaning before, 109 plastic mallet, 112 power failure, 113 removal tool, 111 schedule, 110 tools, 111, 162, 199

Manufacturing information, 9 Master, 52 Master rear connection pan- el, 68 Media valve AxiChrom 300- 1000, 142 Media valve disassembly AxiChrom 300-1000, 142

N Notes and tips, 8 Nozzle tube holder

adjustment, 209

P Pneumatic supply require- ments, 79 Power cables, 76 Power failure, 37 Power requirements, 76

AxiChrom 300-1600 columns Operating Instructions 29065430 AD 329

Index

Prerequisites, 8 Pressure safety equipment

rupture discs, 74 Priming the column, 101 Priming the media valve, 99 PTFE tape on bolts, 112 Purpose of this manual, 6

R Rinse nozzle tip O-ring tool, 205 Risk assessment, 16

S Safety

notices, 8 Safety precautions, 16 Service agreements, 108 Service and preventive mainte- nance, 107 Service frequency, 108 Shutdown procedure, 87 Site requirements, 57 Slurry preparation, 95 Stainless steel bed supports

cleaning, 303 Start up

Storage preparation for storage, 304

Storage conditions, 304

T Tool kit, 111 Top inlet spacer and O-ring AxiChrom 300-800, 163 Top inlet spacer and O-ring AxiChrom 1200, 209 Transport

300-1000 columns, 60 1200-1600 columns, 61 crates for 300-1000 columns, 58 crates for 1200-1600 columns, 60

Tubing diameters, 73 Typographical conventions, 6

U UL

conformity, 11 User access levels, 82 User information

important, 7

V Valve leakage test unit, 206

W Wetting bed supports, 97 Wheel kit

300-600 column, 63 800-1000 column, 63 1200-1600 column, 63

Wire colors, 77

330 AxiChrom 300-1600 columns Operating Instructions 29065430 AD

Index

GE, GE monogram, ÄKTAprocess, AxiChrom, Capto, and UNICORN are trademarks of General Electric Company.

Luer-Lock is a trademark of Becton Dickinson and Company.

MasterMover is a trademark of MasterMover Ltd.

PowerAttack is a trademark of Klaus J. Scholpp.

PROFIBUS is a trademark of PROFIBUS Nutzerorganization e.V.

All other third party trademarks are the property of their respective owner.

Any use of UNICORN is subject to GE Healthcare Standard Software End-User License Agreement for Life Sciences Software Products. A copy of this Standard Software End-User License Agreement is available on request.

All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- sentative for the most current information.

GE Healthcare Europe GmbH Munzinger Strasse 5, D-79111 Freiburg, Germany

GE Healthcare UK Limited Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK

GE Healthcare Bio-Sciences Corp. 100 Results Way, Marlborough, MA 01752, USA

GE Healthcare Dharmacon, Inc. 2650 Crescent Dr., Lafayette, CO 80026, USA

HyClone Laboratories, Inc. 925 W 1800 S, Logan, UT 84321, USA

GE Healthcare Japan Corporation Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073, Japan

For local office contact information, visit www.gelifesciences.com/contact

GE Healthcare Bio-Sciences AB Björkgatan 30 751 84 Uppsala Sweden

www.gelifesciences.com/axichrom

29065430 AD 11/2015 a208

Coverpage
Table of Contents
1 Introduction

1.1 About this manual
1.2 Important user information
1.3 Regulatory information
1.4 Associated documentation

2 Safety instructions

2.1 Safety precautions
2.2 Labels
2.3 Emergency procedures
2.4 Safety zones and access areas
2.5 Crush injury risks
2.6 Recycling information
2.7 Declaration of Hazardous Substances (DoHS)

3 System description

3.1 Column description
3.2 AxiChrom 300-1600 Master description

4 Installation

4.1 Site requirements
4.2 Transport
4.3 Unpacking
4.4 Wheel kits
4.5 Leveling the column
4.6 Connections

5 Operation

5.1 AxiChrom 300-1600 Master and UNICORNsoftware
5.2 Starting up and shutting down the AxiChrom 300-1600 Master
5.3 Cleaning a new column
5.4 Performing a leakage test
5.5 Preparing slurry and buffer
5.6 Priming the column
5.7 Packing the column
5.8 Cleaning a packed column
5.9 Unpacking the column

6 General maintenance and service

6.1 Service and preventive maintenance

6.1.1 Service recommendations
6.1.2 Cleaning before maintenance or service
6.1.3 Maintenance schedules

6.2 General maintenance tools
6.3 Power failure during maintenance
6.4 Putting the column in and out of maintenance mode
6.5 Media valve maintenance
6.6 Changing liquid in the flushing channels
6.7 Changing flushing connector O-rings
6.8 Leakage test

7 Maintenance of 300-1000 columns

7.1 Maintenance tools for 300-1000 columns
7.2 Maintaining the adapter inlet/outlet
7.3 Maintaining columns with stainless steel bed supports

7.3.1 Disassembling the bottom assembly
7.3.2 Disassembling the adapter
7.3.3 Assembling the adapter
7.3.4 Assembling the bottom assembly

7.4 Maintaining columns with plastic bed supports

7.4.1 Disassembling the bottom assembly
7.4.2 Disassembling the adapter
7.4.3 Assembling the adapter
7.4.4 Assembling the bottom assembly

8 Maintenance of 1200-1600 columns

8.1 Maintenance tools for 1200-1600 columns
8.2 Rinse nozzle maintenance
8.3 Lifting and lowering the bottom backing plate
8.4 Maintaining columns with stainless steel bed supports

8.4.1 Disassembling the bottom assembly
8.4.2 Disassembling the adapter
8.4.3 Assembling the adapter
8.4.4 Assembling the bottom assembly

8.5 Maintaining columns with plastic bed supports

8.5.1 Disassembling the bottom assembly
8.5.2 Disassembling the adapter
8.5.3 Assembling the adapter
8.5.4 Assembling the bottom assembly

9 Master maintenance

9.1 AxiChrom 300-1600 Master firmware installation
9.2 Updating AxiChrom 300-1600 Master firmware

10 Cleaning and storage

10.1 Cleaning an empty column
10.2 Cleaning stainless steel bed supports
10.3 Storage

11 Troubleshooting
12 Reference information

12.1 Specifications
12.2 Weights and dimensions
12.3 Spare parts and accessories
12.4 Chemical resistance

12.4.1 Sodium chloride and strong pH solutions

12.5 System recommendations
12.6 Health and Safety Declaration Form
12.7 Ordering information

Index