Lab Week 2

BIOS256 OL, Week 2 Lab

Name:

OL Lab 2: Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infant

Learning Objectives:

· Understand the epithelial model for how glucose is transported across the mammalian small intestine

· Perform a glucose assay method

· Use an animal model to study the transport of materials across the intestine

· Describe the effect of sodium-potassium ATPase blocker on glucose transport by the small intestine

· Explain the effect of manipulations of mucosal concentrations of glucose and sodium on glucose intestinal transport

· Interpret physiological data and apply to clinical cases

In this simulation, you will learn how glucose absorption occurs in the intestine.

You will help treat an infant who has trouble gaining weight. She is experiencing diarrhea, with elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your supervisor, suspects this could be related to the poor glucose absorption. You will use a piece of mouse intestine as your model to study intestinal glucose transport and see how your findings can be used to diagnose human diseases.

Study glucose transport by measuring its concentration:

After clearing out the impurities from the everted intestinal piece, you will expose both the inner (mucosal) and outer (serosal) sides to glucose solutions of different concentrations, a sodium-potassium ATPase blocker, and a sodium-free saline solution.

After these treatments, you will measure the glucose levels on both sides, and analyze your data to understand the active glucose transport through the intestinal epithelium. Also, a step-by-step interactive summary diagram will help you visualize what happens to all the molecules and transporters involved.

Will your findings be able to help make the infant well again?

Part 1: Complete Labster- Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infant

Part 2: Report and Reflection

Purpose: Describe in your own words and in complete sentences, the purpose of this experiment.

Observations: List 2 observations you have made in this simulation.

Answer all the questions below:

1. How does sodium regulate the uptake of glucose into enterocytes? (3 points)

2. In the simulation, why did the infant experience diarrhea due to glucose malabsorption?

(3 points)

3. What causes lactose intolerance? What are the products of lactose breakdown? (2 points)

4. Why is the lumen of the GI tract lined by mucosal membrane? (2 points)

5. Reflection: Reflect on at least 2 key concepts you have learned from this simulation.

How can you apply it to the physiological processes in the body?

Grading Rubric: 

Activity	Deliverable	Points
Part 1	Complete simulation	15
Part 2	Complete lab report and answer questions  · Purpose (1 point)  · Observation (2 points) · Questions (10 points)  · Reflection (2 points)	15
Total	Complete all lab activities	30