Biology Assignment B

Enzymes I Worksheet

(Type in the blanks and submit this worksheet through the assignment tab in iCollege. Paste pictures below the words “Insert Picture Below.” Resize pictures as needed to fit at least two pictures per page.)

Student Name:

Experiment #1 – Enzymes Require Substrates

Which substrate was absent from the reaction under water?

Did the browning reaction occur under water?

Go back to the pre-lab. What is the brown chemical called?

Does the brown chemical mean that the foods are rotting?

Insert Pictures Below

Experiment #2 – Enzyme Denaturation by pH

What were the colors of each of your solutions on pH paper? Which solutions were acidic, basic, or neutral in pH?

(The following questions are about what happens when you soak the fruits in each of the three solutions, then leave them out to brown. Remember, browning = reaction. Not browning = no reaction.)

Did the reaction still occur after soaking in neutral pH?

Did the reaction still occur after soaking in acid?

Did the reaction still occur after soaking in base?

What was happening to the enzyme in the reactions that didn’t occur?

Insert Pictures Below

Experiment #3 – Enzyme Denaturation by Temperature

(Remember, browning = reaction. Not browning = no reaction.)

Did the reaction still occur at room temperature?

Did the reaction still occur after boiling?

Did the reaction still occur after chilling?

Did the reaction still occur after freezing?

What was happening to the enzyme in the reactions that didn’t occur?

Insert Pictures Below