Micro-Unknown Research

Report Form for Gram-Negative Unknown Unknown ID Code:

MORPHOLOGICAL FEATURES (observations and results):

*Gram Stain Reaction: ___________________________________________________

*Cell Morphology and Arrangement:________________________________________

Capsular Stain: _________________________________________________________

*CULTURAL CHARACTERISTICS (observation of colonies on TSA and original unknown broth)

Cultural characteristics in broth: ______________________________

Colony Form:_____________________________________________

Colony Size:______________________________________________

Colony Margin:____________________________________________

Colony Texture____________________________________________

Colony Elevation:__________________________________________

Colony Surface Appearance:__________________________________

Colony Pigment:_(+ or -)___________ Color:_________ H2O soluble?________

PHYSIOLOGICAL CHARACTERISTICS: For lines that just have blanks, include observations and results

*Oxidase: __________________________________

*MacConkey Agar: Growth ( + or -): ______________ Lactose Fermentation: _____________________________

*Glucose Fermentation Broth:_____________________________________________

*Lactose Fermentation Broth:_____________________________________________

*Sucrose Fermentation Broth:_____________________________________________

*Mannitol Fermentation Broth:____________________________________________

Nitrate Reduction ( + or -) :______; observation after addition of:

reagent A&B (color):____________ zinc (color):_____________

*TSIA: Observations: slant (color):__________; butt (color):__________;

Results: Fermentation of Glu: ________; Lac and/or Suc:_________

H2S produced ( + or -)_______; Gas produced ( + or -)________

Motility: _________________________________________

*Required Tests. All other tests are follow-up tests that depend on the results of the required tests. Be sure that you have carried out all of the appropriate follow-up tests that pertain to your organism (see pg 296 in the lab manual).

TSIA reported as:

Urea hydrolysis: ___________________________________

Indole: ___________________________________________

Methyl Red: ______________________________________

Voges-Proskauer: __________________________________

Citrate: __________________________________________

Suspected Unknown: ___________________________________________