Write an MINI-REPORT about the OCEAN ACIDIFICATION PROJECT.

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Index

CRAB Summer 2019 ENVS&100

Summer 2019 ENVS&100
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	pH	CALIPER	MASS	COMMENTS
N Lane	8/3/19	FLAT	1L	5ml	5.70		2.5-3mm 44.94g	POSITIVE CONTROL
	8/5/19	FLAT	1L	5ml	5.70			POSITIVE CONTROL
	8/6/19	FLAT	1L	5ml	5.70		43.33	POSITIVE CONTROL
	8/7/19	FLAT	1L	5ml	5.70		41.90	POSITIVE CONTROL
	8/8/19	FLAT	1L	5ml	5.70		43.31	POSITIVE CONTROL
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	pH	CALIPER (cm)	MASS (grams)	COMMENTS
Sarah, Alexis, Benny	8/1/19	FLAT	1400.00	0.00	8.60			Seawater CONTROL
	8/5/19	FLAT	1400.00	0.00	8.60	0.30	59.76	Seawater CONTROL
	8/6/19	FLAT	1400.00	0.00	8.60	0.30	59.20	Seawater CONTROL
	8/7/19	FLAT	1400.00	0.00	8.60	0.30	55.11	Seawater CONTROL
	8/8/19	FLAT	1400.00	0.00	8.60	0.30	55.82	Seawater CONTROL
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	pH	CALIPER (cm)	MASS (grams)	COMMENTS
Sarah, Alexis, Benny	8/1/19	FLAT	1400.00	60.00	6.85	0.30
	8/5/19	FLAT	1400.00	60.00	6.85	0.30	53.44
	8/6/19	FLAT	1400.00	60.00	6.85	0.30	51.73
	8/7/19	FLAT	1400.00	60.00	6.85	0.20	47.59
	8/8/19	FLAT	1400.00	60.00	6.85	0.20	46.70
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	pH	CALIPER	MASS	COMMENTS
Boyuan Shen Ruoting Fang	8/1/19	FLAT	1400.00	40.00	7.45	0.3cm	58.8g	crab hard
	8/5/19	FLAT	1400.00	20.00	7.45	0.3cm	57.86g	bad smell and the crab shell's weight is less than first day.
	8/6/19	FLAT	1400.00	20.00	7.45	0.2cm	56.69g	weight less, tinner
	8/7/19	FLAT	1400.00	20.00	7.45	0.12cm	52.6g	crab shell's weight less, the crab shell is thiner
	8/8/19	FLAT	1400.00	20.00	7.45	0.1cm	52.33g	crab shell is softer and easy to break
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	pH	CALIPER	MASS	COMMENTS
Yiwei, Yuhang, Lejun, Jingxuan	8/1/19	upright	1300.00	30.00	7.60	0.28	47.8g	crabshell hard
	8/5/19	upright	1300.00	30.00	7.60	0.28	47.6g	became soft
	8/6/19	upright	1300.00	30.00	7.60	0.27	47.2g	shed a litle
	8/7/19	upright	1300.00	30.00	7.60	0.25	47.2g	nothing changed
	8/8/19	upright	1300.00	30.00	7.60	0.19	47.0g	crabshell is the thinnest
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME(ml)	DROPS	pH	CALIPER	MASS	COMMENTS
Ryder Hsu, Napolean Yong, Tiangi Gong	8/1/19	UPRIGHT	1000.00	15.00	7.60	0.2 cm	40.21 g
	8/5/19	UPRIGHT	1000.00	15.00	7.60	0.1 cm		It has become softer
	8/6/19	UPRIGHT	1000.00	15.00	7.60	0.1 cm	41.46 g	Slightly more unlnarble
	8/7/19	UPRIGHT	1000.00	15.00	7.60	0.1 cm	41.58 g	it's much more fragile
	8/8/19	UPRIGHT	1000.00	15.00	7.60	0.05 cm	41.53 g	It has become much softer campare to the first day
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	pH	CALIPER	MASS	COMMENTS
Yang HU, Jiaxin Zhang,	8/1/19	FLAT	1400.00	20.00	8.50	0.30		start test
Wenlong Zeng, Ziyan Ma	8/5/19	FLAT	1400.00	20.00	8.08	0.30	28.84g	it was broken because the shell is soft
	8/6/19	FLAT	1400.00	20.00	8.02	0.20	27.8g	weight reduced and the shell became thinner
	8/7/19	FLAT	1400.00	20.00	8.05	0.10	23.53g	weight reduced and the shell easy to break and became thinner
	8/8/19	FLAT	1400.00	20.00	8.05	0.08	23.93g	weight increased because it is wet, and the shell keep becoming thinner
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	pH	CALIPER	MASS	COMMENTS
Zilong Tang, Yuhui Zhang	8/1/19	UPRIGHT	1000.00	50.00	6.73	0.25cm
	8/5/19	UPRIGHT	1000.00	50.00	6.73	0.21cm	27.84g	Inside of the shell turns a little bit yellow
	8/6/19	UPRIGHT	1000.00	50.00	6.73	0.15cm	29.75g	Hairs on the shell start shedding
	8/7/19	UPRIGHT	1000.00	50.00	6.73	0.10cm	27.65g	The shell gets softer, and a crack appear.
	8/8/19	UPRIGHT	1000.00	50.00	6.73	0.10cm	27.54g	The shell has lost all its fur, and gets softer.

Graphs ENVS&100

Summer 2019 ENVS&100
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	DATE	pH	CALIPER cm	Control	pH		DATE	Mass(g)	COMMENTS
												pH 5
N Lane	8/3/19	FLAT	1L	5ml	8/3/19	5.70	0.30		5.70		8/3/19	44.94	POSITIVE CONTROL
	8/5/19	FLAT	1L	5ml	8/5/19	5.70	0.30		5.70		8/5/19		POSITIVE CONTROL
	8/6/19	FLAT	1L	5ml	8/6/19	5.70	0.30		5.70		8/6/19	43.33	POSITIVE CONTROL
	8/7/19	FLAT	1L	5ml	8/7/19	5.70	0.30		5.70		8/7/19	41.90	POSITIVE CONTROL
	8/8/19	FLAT	1L	5ml	8/8/19	5.70	0.30		5.70		8/8/19	43.31	POSITIVE CONTROL
									5.70
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	DATE	pH	CALIPER (cm)	Control	pH	Control pH 8.6	DATE	MASS (grams)	COMMENTS
Sarah, Alexis, Benny	8/1/19	FLAT	1400.00	0.00	8/1/19	8.60			8.60		8/1/19		Seawater CONTROL
	8/5/19	FLAT	1400.00	0.00	8/5/19	8.60	0.30		8.60		8/5/19	59.76	Seawater CONTROL
	8/6/19	FLAT	1400.00	0.00	8/6/19	8.60	0.30		8.60		8/6/19	59.20	Seawater CONTROL
	8/7/19	FLAT	1400.00	0.00	8/7/19	8.60	0.30		8.60		8/7/19	55.11	Seawater CONTROL
	8/8/19	FLAT	1400.00	0.00	8/8/19	8.60	0.30		8.60		8/8/19	55.82	Seawater CONTROL
														DATE	Mass(g)
															Control pH 8.6	Flat pH 8	Flat pH 8.05	Upright pH 7.6	Upright pH 7.6	Flat pH 6.85	Upright pH 6.73	Control pH 5
														8/3/19		58.8		47.80	40.21			44.94
														8/5/19	59.76	57.86	28.84	47.60		53.44	27.84
														8/6/19	59.20	56.69	27.80	47.20	41.46	51.73	29.75	43.33
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	DATE	pH	CALIPER		pH		DATE	MASS	COMMENTS	8/7/19	55.11	52.60	23.53	47.20	41.58	47.59	27.65	41.90
Boyuan Shen Ruoting Fang	8/5/19	FLAT	1400.00	20.00	8/5/19	8?	0.30		8.00		8/5/19	57.86	bad smell and the crab shell's weight is less than first day.	8/8/19	55.82	52.33	23.93	47.00	41.53	46.70	27.54	43.31
	8/6/19	FLAT	1400.00	20.00	8/6/19		0.20		8.00		8/6/19	56.69	weight less, tinner			Boyyan,Ruoting	Wenlong & others	Yiwei & others	RyderNapoleanTiangi	SarahAlexisBenny	ZilongYuhui
	8/7/19	FLAT	1400.00	20.00	8/7/19		0.12		8.00		8/7/19	52.60	crab shell's weight less, the crab shell is thiner
	8/8/19	FLAT	1400.00	20.00	8/8/19		0.10		8.00		8/8/19	52.33	crab shell is softer and easy to break
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	DATE	pH	CALIPER		pH 8.05 average		DATE	MASS	COMMENTS
Yang HU, Jiaxin Zhang,	8/1/19	FLAT	1400.00	20.00	8/1/19	8.50	0.30		8.50		8/1/19		start test
Wenlong Zeng, Ziyan Ma	8/5/19	FLAT	1400.00	20.00	8/5/19	8.08	0.30		8.08		8/5/19	28.84	it was broken because the shell is soft
	8/6/19	FLAT	1400.00	20.00	8/6/19	8.02	0.20		8.02		8/6/19	27.80	weight reduced and the shell became thinner
	8/7/19	FLAT	1400.00	20.00	8/7/19	8.05	0.10		8.05		8/7/19	23.53	weight reduced and the shell easy to break and became thinner
	8/8/19	FLAT	1400.00	20.00	8/8/19	8.05	0.08		8.05		8/8/19	23.93	weight increased because it is wet, and the shell keep becoming thinner
Boyuan Shen Ruoting Fang	8/1/19	FLAT	1400.00	40.00	8/1/19	7.45	0.30		7.45		8/1/19	58.80	crab hard
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	DATE	pH	CALIPER (cm)		pH	Flat pH 6.85	DATE	MASS (grams)	COMMENTS
Sarah, Alexis, Benny	8/1/19	FLAT	1400.00	60.00	8/1/19	6.85	0.30		6.85		8/1/19
	8/5/19	FLAT	1400.00	60.00	8/5/19	6.85	0.30		6.85		8/5/19	53.44
	8/6/19	FLAT	1400.00	60.00	8/6/19	6.85	0.30		6.85		8/6/19	51.73
	8/7/19	FLAT	1400.00	60.00	8/7/19	6.85	0.20		6.85		8/7/19	47.59
	8/8/19	FLAT	1400.00	60.00	8/8/19	6.85	0.20		6.85		8/8/19	46.70
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	DATE	pH	CALIPER		pH		DATE	MASS	COMMENTS
Yiwei, Yuhang, Lejun, Jingxuan	8/1/19	upright	1300.00	30.00	8/1/19	7.60	0.28		7.60		8/1/19	47.80	crabshell hard
	8/5/19	upright	1300.00	30.00	8/5/19	7.60	0.28				8/5/19	47.60	became soft
	8/6/19	upright	1300.00	30.00	8/6/19	7.60	0.27				8/6/19	47.20	shed a litle
	8/7/19	upright	1300.00	30.00	8/7/19	7.60	0.25				8/7/19	47.20	nothing changed
	8/8/19	upright	1300.00	30.00	8/8/19	7.60	0.19				8/8/19	47.00	crabshell is the thinnest
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME(ml)	DROPS	DATE	pH	CALIPER		pH		DATE	MASS	COMMENTS
Ryder Hsu, Napolean Yong, Tiangi Gong	8/1/19	UPRIGHT	1000.00	15.00	8/1/19	7.60	0.20		7.60		8/1/19	40.21
	8/5/19	UPRIGHT	1000.00	15.00	8/5/19	7.60	0.10				8/5/19		It has become softer
	8/6/19	UPRIGHT	1000.00	15.00	8/6/19	7.60	0.10				8/6/19	41.46	Slightly more unlnarble
	8/7/19	UPRIGHT	1000.00	15.00	8/7/19	7.60	0.10				8/7/19	41.58	it's much more fragile
	8/8/19	UPRIGHT	1000.00	15.00	8/8/19	7.60	0.05				8/8/19	41.53	It has become much softer campare to the first day
NAME	DATE	Trial# UPRIGHT or FLAT	WATER VOLUME	DROPS	DATE	pH	CALIPER		pH		DATE	MASS	COMMENTS
Zilong Tang, Yuhui Zhang	8/1/19	UPRIGHT	1000.00	50.00	8/1/19	6.73	0.25		6.73		8/1/19
	8/5/19	UPRIGHT	1000.00	50.00	8/5/19	6.73	0.21		6.73		8/5/19	27.84	Inside of the shell turns a little bit yellow
	8/6/19	UPRIGHT	1000.00	50.00	8/6/19	6.73	0.15		6.73		8/6/19	29.75	Hairs on the shell start shedding
	8/7/19	UPRIGHT	1000.00	50.00	8/7/19	6.73	0.10		6.73		8/7/19	27.65	The shell gets softer, and a crack appear.
	8/8/19	UPRIGHT	1000.00	50.00	8/8/19	6.73	0.10		6.73		8/8/19	27.54	The shell has lost all its fur, and gets softer.

Dungeness Crab Shell thickness changes at different pH seawater over 1 week

pH 8/3/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 8/1/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/ 8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 5.7 5.7 5.7 5.7 5.7 0 8.6 8.6 8.6 8.6 8.6 0 0 0 8.5 8.08 8.02 8.0500000000000007 8.0500000000000007 7.45 0 6.85 6.85 6.85 6.85 6.85 0 7.6 7.6 7.6 7.6 7.6 0 7.6 7.6 7.6 7.6 7.6 0 6.73 6.73 6.73 6.73 6.73 CALIPER cm 8/3/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 8/1/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 DATE 8/1/19 8/5/19 8/6/19 8/7/19 8/8/19 0.3 0.3 0.3 0.3 0.3 0 0.3 0.3 0.3 0.3 0 0.3 0.2 0.12 0.1 0 0.3 0.3 0.2 0.1 0.08 0.3 0 0.3 0.3 0.3 0.2 0.2 0 0.28000000000000003 0.28000000000000003 0.27 0.25 0.19 0 0.2 0.1 0.1 0.1 0.05 0 0.25 0.21 0.15 0.1 0.1

Change in Dungeness crab shell mass at pH 5 to 8.6

Mass(g) Control pH 8.6 43680 43682 43683 43684 43685 59.76 59.2 55.11 55.82 Mass(g) Flat pH 8 43680 43682 43683 43684 43685 58.8 57.86 56.69 52.6 52.33 Mass(g) Flat pH 8.05 43680 43682 43683 43684 43685 28.84 27.8 23.53 23.93 Mass(g) Upright pH 7.6 43680 43682 43683 43684 43685 47.8 47.6 47.2 47.2 47 Mass(g) Upright pH 7.6 43680 43682 43683 43684 43685 40.21 41.46 41.58 41.53 Mass(g) Flat pH 6.85 43680 43682 43683 43684 43685 53.44 51.73 47.59 46.7 Mass(g) Upright pH 6.73 43680 43682 43683 43684 43685 27.84 29.75 27.65 27.54 Mass(g) Control pH 5 43680 43682 43683 43684 43685 44.94 43.33 41.9 43.31

Change in Dungeness Crab shell mass at different pH levels

8/3/19

Control pH 8.6 Flat pH 8 Flat pH 8.05 Upright pH 7.6 Upright pH 7.6 Flat pH 6.85 Upright pH 6.73 Control pH 5 Mass(g) 58.8 47.8 40.21 44.94 8/5/19

Control pH 8.6 Flat pH 8 Flat pH 8.05 Upright pH 7.6 Upright pH 7.6 Flat pH 6.85 Upright pH 6.73 Control pH 5 Mass(g) 59.76 57.86 28.84 47.6 53.44 27.84 8/6/19

Control pH 8.6 Flat pH 8 Flat pH 8.05 Upright pH 7.6 Upright pH 7.6 Flat pH 6.85 Upright pH 6.73 Control pH 5 Mass(g) 59.2 56.69 27.8 47.2 41.46 51.73 29.75 43.33 8/7/19

Control pH 8.6 Flat pH 8 Flat pH 8.05 Upright pH 7.6 Upright pH 7.6 Flat pH 6.85 Upright pH 6.73 Control pH 5 Mass(g) 55.11 52.6 23.53 47.2 41.58 47.59 27.65 41.9 8/8/19

Control pH 8.6 Flat pH 8 Flat pH 8.05 Upright pH 7.6 Upright pH 7.6 Flat pH 6.85 Upright pH 6.73 Control pH 5 Mass(g) 55.82 52.33 23.93 47 41.53 46.7 27.54 43.31

Sheet2

Sheet3

PAGODA Summer 2019 BIOL&100

Summer 2019 BIOL&100
Pagoda Cup Coral Study
NAME	DATE	pH	In Tank: Behavior (color, openness)	Experimental: Behavior (color, openness, time of change)	Return to Tank: Behavior (color, openness, time of change)
	8/5/19		27C	22C
	8/6/19		27C	25C Waterbath
	8/7/19
	8/8/19

GRAPES Summer 2019 BIOL&100

Summer 2019 BIOL&100
Grapes Study
NAME	DATE	Control pH, temperature, notes	Skin & Pulp pH, temperature, notes	Skin only pH, temperature, notes	Pulp only pH, temperature, notes
	8/5/19
	8/6/19
	8/7/19
	8/8/19