Final Lab Report for Microbiology
ThaliaRamos99
ClassActivity6.docxCLASS ACTIVITY WORKSHEET
Section: U01
Group: #5
Environment: Plant
Name: Thalia Ramos Gigato
ID: 6141550
Date: 03/15/2022
Lab Title: Advanced Biochemical Testing and Bergey’s Manual of Bacteriology Identification.
Table 1:
|
Test |
Picture |
Result |
Description |
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Mannitol Salt Agar (MSA)
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Reaction: NOT APPLICABLE No colonies grew on the plate.
Color: clear
pH: Not applicable
Other: No presence of colonies.
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Metabolic reaction: No bacteria was able to grow in either differential or selective medium. Enzymatic activity: Based on what we can observe no bacteria grew on the plate meaning that it wasn’t halophile or halotolerant. Other: No acidic fermentation of mannitol sugar present.
Type of bacteria based on selective or differential properties. Not applicable because no bacteria grew on the plate.
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Eosin-Y Methylene Blue Agar (EMB) |
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Reaction: positive/negative? Negative Color: cream light purple pH: acidic Other: Can really observe well on the picture but it was a creamy light purple color.
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Metabolic reaction: The agar is mainly used as a differential medium, but it also contains lactose that when fermented gives a creamy purple color as a result. Enzymatic activity: Lactose was not fermented. Type of bacteria based on selective or differential properties. Gram-negative non-lactose utilizer.
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Blood Agar |
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Reaction: Negative
Color: white/transparent
pH: alkaline
Other: Gamma-hemolysis
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Metabolic reaction: Bacteria grew on the plate, but it didn’t cause hemolysis of red blood cells. Enzymatic activity: Differential medium is used in the plate to distinguish the two types of hemolysis, but no destruction of red blood cells occurred. Type of bacteria based on selective or differential properties. Gram negative bacteria.
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MacConkey Agar |
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Reaction: Positive Color: pink
pH: alkaline
Other: In the picture doesn’t look too pink.
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Metabolic reaction: The medium is both selective and differential but since it turned to be pink the bacteria used differential medium because lactose fermenting colonies are present. Enzymatic activity: Lactose was fermented. Type of bacteria based on selective or differential properties. Gram-negative
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Table 2:
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API Test |
Picture |
Reactions |
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Colony morphology:
Oxidase:
Catalase:
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List each test and its result- including OX (positive/negative and reaction that occurred) ONPG: Negative DAH: Positive LDC: Negative ODC: Negative CIT: Negative H2S: Negative URE: Positive. It wasn’t yellow but since it had a color change it’s considered positive. TDA: Positive IND: Negative VP: Negative GEL: Negative GLU: Positive. No color change indicating the presence of ammonium. MAN: Negative INO: Negative SOR: Negative RHA: Negative SAC: Positive MEL: Negative AMY: Negative ARA: Positive List the 7-digit code: 2034022
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Negative reaction Color: no color change pH: acidic
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Positive reaction Color: white pH: alkaline (basic) Other: catalase present
Describe your results from the API Web page and your conclusion on your bacteria’s identification Based on the results and the number we obtained we can conclude that our bacteria is Proteus penneri. This bacteria is rod-shaped, catalase positive, and Gram-negative bacteria.
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Part 3:
Include a screenshot of the Bergey’s manual dichotomous key(s) used to identify your bacteria with markings showing the pathways taken to identify your bacteria and the final identification circled/highlighted.
Final Identification of unknown bacteria: Proteus penneri
