Final Lab Report for Microbiology
ThaliaRamos99
ClassActivity2.docxActivity #2
Section: U01
Lab Title: Isolation and Identification of Bacteria from Wild Samples.
Date: 02/08/2022
Student Name: Thalia Ramos Gigato
Student ID: 6141550
Table 1: Population survey of environmental sample
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Environment |
Picture |
Description |
Dilution Factor |
Viable Titer |
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Plant
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Population Survey Unique colonies: 22
Colony Morphology: Size: small Shape: punctiform Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque Other: spread out, more in the right side. |
102 |
CFU: 22
Viable Titer: 2.2x10^4 CFU/mL Calculations.
(22CFU/0.1mL) x10^2 = 22,000 = 2.2x10^4 CFU/mL
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Population Survey Unique colonies: N/A
Colony Morphology: Size: N/A Shape: N/A Color: N/A Margins: N/A Elevation: N/A Surface: N/A Opacity: N/A Other: No colonies presented in the plate. |
104 |
CFU: No colonies viable Titer: Cannot be calculated Calculations N/A |
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Population Survey Unique colonies: 33
Colony Morphology Size: small Shape: punctiform Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque Other: The colonies appear smaller than the colonies on the plate 10^2 |
106 |
CFU: 33 Viable Titer 3.3x10^8 CFU/mL Calculations (33 CFU/0.1mL) x10^6= 330,000,000= 3.3x10^8 CFU/mL |
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The first plate has many colonies but not as much as the third plate, while the second plate doesn’t have any colonies present, just appear sweaty. Also, the colonies on the 10^6 plate are smaller than the ones presented in the 10^2 plate. |
Table 2: Gram Stains characterization of chosen colonies
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Isolated Colony |
Dilution |
Picture |
Description |
Picture |
Description |
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Section: U01 Group: 5
Colony #1
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10^-2 |
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Colony Morphology: Size: small Shape: circular Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque
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40x objective
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Cellular Morphology: Size: small Shape: rods Arrangement: clustered, bacillus Color: pink Staining characteristics: The stain was pink indicating being Gram negative.
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100x objective
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Colony #2 |
10^2 |
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Colony Morphology: Size: small Shape: circular Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque
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40x objective
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Cellular Morphology: Size: small Shape: cocci Arrangement: clumped, streptococcus Color: pink Staining characteristics: Since the stain turned pink indicates being Gram negative.
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100x objective
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Table 3: Other characterized colonies
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Isolated Colony |
Dilution |
Picture |
Description |
Picture |
Description |
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Colony #3 |
10^2 |
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Colony Morphology: Size: small Shape: circular Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque
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40x objective
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Cellular Morphology: Size: small Shape: rods Arrangement: clustered, bacillus Color: pink Staining Characteristics: Since the stain turned pink indicates that it might be Gram negative. Other: After staining the bacteria turned pink which indicates possible being Gram negative but there are always the possibility of some cross contamination altering the results. |
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100x objective
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Colony #4 |
10^2 |
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Colony Morphology: Size: small Shape: circular Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque
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40x objective
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Cellular Morphology: Size: small Shape: rods Arrangement: clustered, streptobacillus Color: purple Staining Characteristics: After staining the bacteria turned purple which indicates being Gram positive. Other: Since the rest of the bacteria have stained pink and this is the only one turning purple it could mean there was some cross contamination in the process. |
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100x objective
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Colony #5 |
10^2 |
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Colony Morphology: Size: small Shape: circular Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque
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40x objective
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Cellular Morphology: Size: small Shape: rods Arrangement: clustered, bacillus Color: pink Staining Characteristics: Since the bacteria appears pink it could indicate being Gram negative. Other: The bacteria appear to be Gram negative but there could be some cross contamination altering the results. |
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100x objective
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