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BIO 122

Friday 1:30 – 4 pm

Fall 2017

Lab 8: Design Your Own Experiment Cellular Respiration and Fermentation Part II

Cellular Respiration or Fermentation?

Student Name:

Cellular Respiration/ Fermentation:

Hypothesis:

Materials Needed to Conduct Experiment:

Predictions for Lab:

Briyani, Hannah, Lynn

Alcoholic Fermentation

What environmental conditions are optimal for alcoholic fermentation, with altered temperature ranges?

4 Erlenmeyer flasks, 4 rubber tubings, 4 donut rings, 4 1 mL pipettes, 4 binder clips, 5 mL pipettes, 10% glucose solution, 14% yeast solution, DI water, water baths, thermometer, ice bucket, ice

Alcoholic fermentation will work the quickest and the more efficiently in a 37 degree Celsius water bath because it is the physiological temperature yeast work in to produce CO2

Shelcie, Kyara, Xiaogang, Faisal

Alcoholic Fermentation

Will fermentation change with different types of yeast?

Quick rise yeast, Candida milleri (sourdough), champagne yeast, 4 Erlenmeyer flasks, 4 rubber tubings, 4 donut rings, 4 1 mL pipettes, 4 binder clips, 5 mL pipettes, 10% glucose solution, DI water

Alcoholic fermentation will work the quickest and the more efficiently when using quick rise yeast for commercial bread making and will produce the most CO2

Israel, Pam, Rachel

Alcoholic Fermentation

Would other substrates be as effective as glucose in alcoholic fermentation?

Fructose, Splenda, pyruvate, 10% glucose solution, 4 Erlenmeyer flasks, 4 rubber tubings, 4 donut rings, 4 1 mL pipettes, 4 binder clips, 5 mL pipettes, 14% yeast solution, DI water

The most successful substrate in place of glucose that will work to efficiently to ferment and produce CO2 is fructose because the molecular structure of both fructose and glucose, both natural sugar substances, are similar.

Kevin, Rick, and Casey

Cellular Respiration

Does the food preservative sodium benzoate inhibit cellular respiration?

Standard test tubes, test tube rack, succinate, DPIP, ice bucket, mitochondrial suspension, 1 mL pipettes, 5 mL pipettes, sodium benzoate

Because sodium benzoate has the ability to deprive cells of oxygen it will inhibit cellular respiration from occurring.