Identification of unknown Lab

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Student: Manoj

Phases Complete 7 of 7 phases.

Phase Resets Phase 1: 0 Phase 2: 0 Phase 3: 0 Phase 4: 0 Phase 5: 0 Phase 6: 0 Phase 7: 0 Phase 8: 0

CASE REPORT: For most of the morning, Tracey was experiencing low pelvic pain and a burning sensation each time she urinated. She recognized the symptoms of a bladder infection and made an appointment with the urgent care clinic for later that day. A urinalysis showed an abundance of white blood cells, suggesting a urinary tract infection (UTI). A specimen of clean-catch urine was sent to the microbiology lab where a sterile calibrated loop was used to deliver and streak out 1 microliter of urine on an agar plate, which yielded colonies too numerous to count. An inoculation and overnight incubation of the urine into sterile broth yielded heavy growth.

Case Introduction Questions Based on the information in the case, what organism(s) could or should be suspected to cause this scenario? Select all that apply. Escherichia coli Staphylococcus aureus Staphylococcus saprophyticus Streptococcus agalactiae

Now that a pure culture has been isolated for you, what would be a good starting procedure to begin classifying your unknown organism?

Gram staining

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Collected Lab Data Test Result

Gram staining

Gram (+) cocci in clusters

Catalase test Bubbles were noted on slide, unknown bacteria was concluded to be catalase (+)

Mannitol salt agar test Pink reaction was noted on MSA plate, unknown bacteria grew in high salt and was concluded to be unable to ferment mannitol

Novobiocin test No zone of inhibition was noted on plate, unknown bacteria was concluded to be novobiocin (Resistant)

Unknown bacterium identification Staphylococcus saprophyticus

Completed Dichotomous Key

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