Lab

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CASE REPORT: Over the past 2 days, Jenny, an active healthy 3 yr-old who had successfully potty-trained a year earlier, began to have “accidents” along with appearing in pain and straining when going to the bathroom. Her mom noted that Jenny’s urine had a faint pink tinge. A trip to the pediatrician quickly confirmed that Jenny had a urinary tract infection (UTI). A urine specimen was collected and sent to the microbiology laboratory for identification. The laboratory performed a quantitative culture and pure culture. The next morning, the lab called the pediatrician, indicating that Jenny’s urine quantitative culture grew >100,000 CFU/mL of bacteria. The laboratory team also isolated the organism into pure culture and was provided to you on a nutrient agar plate and in a liquid broth culture tube for further investigation.

Student: kusum

Case Introduction Questions Based on the information in the case, what organism(s) could or should be suspected to cause this scenario? Select all that apply. Proteus vulgaris Enterococcus aerogenes Escherichia coli Alcaligenes faecalis

Now that a pure culture has been isolated for you, what would be a good starting procedure to begin classifying your unknown organism?

Gram staining

Collected Lab Data Test Result

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Test Result

Gram staining

Gram (-) bacilli

Oxidase test No color was noted on swab, concluded as oxidase (-)

Lactose fermentation Pink-to-purple colonies were noted on MacConkey agar, unknown bacteria was concluded as lactose fermentation (+)

Methyl red test Red reaction was noted in MR-VP tube, unknown bacteria was concluded to be methyl red (+)

Indole test Red ring was noted in indole tube after Kovacs reagent addition, unknown bacteria was concluded to be indole (+)

Unknown bacterium identification Escherichia coli

Completed Dichotomous Key

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