BIOL 362 Unknown Project

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1

BIOL 362 Unknown Project Name______________________________________________

Tube ID_______________

Describe the colony morphology of your isolated and pure bacterium. (Include all 6 categories except for size

and consistency) (12 points)

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Gram stain morphology (6 points) _____________________________________________________________

Does the organism sporulate? (2 points) _____________________

Record the results of the following tests (36 points):

Capsule Stain______________________________________________________________________________

Glucose Fermentation _______________________________________________________________________

Lactose Fermentation________________________________________________________________________

Sucrose Fermentation________________________________________________________________________

Mannitol Fermentation_______________________________________________________________________

Urease____________________________________________________________________________________

Citrate____________________________________________________________________________________

MSA_____________________________________________________________________________________

Coagulase_________________________________________________________________________________

2

EMB agar__________________________________________________________________________________

Motility___________________________________________________________________________________

Hemolysis_________________________________________________________________________________

TSI______________________________________________________________________________________

Oxidase___________________________________________________________________________________

Catalase___________________________________________________________________________________

Gelatinase_________________________________________________________________________________

Lipase_________________________________________________________________________________

HE____________________________________________________________________________________

Explain HOW you determined the identity of your organism using a flow chart or dichotomous key (15 pts) and

explanation (25 pts).

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What is the genus of your organism? (4 points) ____________________________________

What is the species of your organism? ___________________________________