Lab 127 Day 2

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BiochemicalTestStudentDatabase-Fall2020.pdf

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Biochemical Test Student Database: Results for Select Bacterial Species SIDE 1

Number shown is number of tests having that result Biology 127 Online– Fall 2020

Organism G

ra m

s ta

in (

1 )

G lu

c o s e

F e rm

e n ta

ti o n (

2 )

L a c to

s e

F e rm

e n ta

ti o n (

2 )

S u c ro

s e

F e rm

e n ta

ti o n (

2 )

M a

n n o s e

F e rm

e n ta

ti o n (

2 )

O x id

a s e

C a ta

la s e

P ro

d u c ti o n

N it ra

R e d u c ti o n

M o

ti li ty

S ta

rc h

H y d ro

ly s is

C a s e in

H y d ro

ly s is

G e la

ti n

H y d ro

ly s is

U re

a H

y d ro

ly s is

H 2 S

P ro

d u c ti o n

T ri p le

S u g a r

Ir o n a

g a r

(3 )

In d o

P ro

d u c ti o n

M e

th y l R

e d

V o g e s -

P ro

s k a u e r

C it ra

U ti li z a ti o n

Bacillus laterosporus

GPB

+/+ 0 +/- 6 -/- 0

+/+ 0 +/- 1 -/- 5

+/+ 0 +/- 2 -/- 4

+/+ 0 +/- 6 -/- 0

+6 -0

+5 -1

+6 -0

+5 -0

+0 -4

+4 -0

+0 -4

K/A 6

+0 -7

+6 -1

+0 -7

Bacillus megaterium

GPB

+/+ 0 +/- 6 -/- 0

+/+ 0 +/- 0 -/- 6

+/+ 0 +/- 6 -/- 0

+4 -2

+6 -0

+10 -0

+9 -1

+0 -10

K/A 6 A/A 1

+0 -7

+7 -0

+2 -5

Bacillus subtilis

GPB

+/+ 0 +/- 6 -/- 0

+/+ 0 -/+ 0 -/- 6

+/+ 0 +/- 6 -/- 0

+/+ 0 +/- 0 -/- 6

+4 -2

+6 -0

+5 -1

+10 -0

+0 -10

K/K 4 K/A 2

+0 -6

+6 -2

+4 -2

Corynebacterium diphtheriae

GPB

+/+ 0 +/- 7 -/- 0

+/+ 0 -/+ 0 -/- 7

+/+ 0 +/- 0 -/- 7

+/+ 0 +/- 7 -/- 0

+0 -7

+7 -0

+0 -7

+0 -4

K/A 7

+0 -7

+2 -5

+0 -7

Corynebacterium xerosis

GPB

+/+ 0 +/- 8 -/- 0

+/+ 0 +/- 0 -/- 8

+/+ 0 +/- 8 -/- 0

+/+ 0 +/- 7 -/- 1

+0 -8

+8 -0

+0 -8

+0 -4

A/A 5 A/K 2

+0 -7

+7 -0

+6 -1

+0 -7

Listeria grayi

GPB

+/+ 1 +/- 6 -/- 0

+/+ 0 +/- 7 -/- 0

+/+ 1 +/- 6 -/- 0

+/+ 0 +/- 7 -/- 0

+0 -7

+7 -0

+0 -7

+7 -0

+0 -4

+4 -0

+0 -4

A/A 7

+0 -7

+7 -0

+6 -1

+0 -7

Mycobacterium smegmatis

GPB

+/+ 0 +/- 0 -/- 7

+0 -7

+6 -1

+0 -7

+0 -10

+9 -1

+0 -10

K/K 7

+0 -7

(1) GNB, Gram-negative bacillus; GPB, Gram-positive bacillus

(2) Carbohydrate fermentation results shown as acid/gas

(3) TSI slant/butt - A, acid; K, alkaline; G, gas production, H2S, hydrogen sulfide production

Biochemical Test Student Database: Results for Select Bacterial Species SIDE 2

Number shown is number of tests having that result Biology 127 Online – Fall 2020

Organism G

ra m

S ta

in (

1 )

G lu

c o s e

F e rm

e n ta

ti o n (

2 )

L a c to

s e

F e rm

e n ta

ti o n (

2 )

S u c ro

s e

F e rm

e n ta

ti o n (

2 )

M a

n n o s e

F e rm

e n ta

ti o n (

2 )

O x id

a s e

C a ta

la s e

P ro

d u c ti o n

N it ra

R e d u c ti o n

M o

ti li ty

S ta

rc h

H y d ro

ly s is

C a s e in

H y d ro

ly s is

G e la

ti n

H y d ro

ly s is

U re

a H

y d ro

ly s is

H 2 S

P ro

d u c ti o n

T ri p le

S u g a r

Ir o n a

g a r

(3 )

In d o

P ro

d u c ti o n

M e

th y l R

e d

V o g e s -

P ro

s k a u e r

C it ra

U ti li z a ti o n

Aeromonas hydrophila

GNB

+/+ 6 +/- 4 -/- 0

+/+ 0 +/- 0 -/- 10

+/+ 10 +/- 0 -/- 0

+/+ 9 +/- 1 -/- 0

+10 -0

+0 -10

K/A,G 6 K/A 1

A/A,G 1

+8 -0

+0 -8

+8 -0

+0 -8

Alcaligenes faecalis

GNB

+/+ 0 +/- 0 -/- 10

+10 -0

+0 -10

+9 -1

+0 -4

K/K 5

+0 -5

+5 -0

Citrobacter freundii

GNB

+/+ 5 +/- 0 -/- 0

+0 -5

+5 -0

+0 -10

+10 -0

A/A,G,H2S 5 A/A, G 1

+0 -6

+6 -0

+0 -6

+6 -0

Enterobacter aerogenes

GNB

+/+ 5 +/- 0 -/- 0

+/+ 5 +/- 0 -/+ 0

+0 -5

+5 -0

+0 -4

A/A,G 6

+0 -6

+6 -0

Escherichia coli

GNB

+/+ 4 +/- 0 -/- 0

+/+ 0 +/- 0 -/- 4

+/+ 4 +/- 0 -/- 0

+0 -4

+4 -0

+0 -4

+1 -3

+0 -4

A/A,G 8

+8 -0

+0 -8

Klebsiella pneumoniae

GNB

+/+ 5 +/- 0 -/- 0

+0 -5

+5 -0

+1 -4

+0 -10

+9 -1

+0 -10

A/A,G 6

+0 -6

+6 -0

+0 -6

+6 -0

Morganella morganii

GNB

+/+ 5 +/- 0 -/- 0

+/+ 0 +/- 0 -/- 5

+/+ 0 -/+ 0 -/- 5

+/+ 4 +/- 1 -/- 0

+0 -5

+5 -0

+0 -10

+10 -0

+1 -9

K/A,G 7

+7 -0

+6 -1

+0 -7

Proteus vulgaris

GNB

+/+ 1 +/- 4 -/- 0

+/+ 0 +/- 0 -/- 5

+/+ 2 +/- 3 -/- 0

+/+ 0 +/- 0 -/- 5

+0 -5

+5 -0

+4 -1

+0 -9

+8 -1

+0 -9

+9 -0

A/?,H2S 4 A/A,H2S 3

+7 -0

+0 -7

Pseudomonas aeruginosa

GNB

+/+ 0 +/- 0 -/- 10

+10 -0

+0 -4

+4 -0

+3 -1

+0 -4

K/K 6

+0 -6

+6 -0

Salmonella pullorum

GNB

+/+ 7 +/- 0 -/- 0

+/+ 0 +/- 0 -/- 7

+/+ 7 +/- 0 -/- 0

+0 -7

+10 -0

+6 -1

+2 -5

+0 -10

+4 -8

+0 -10

+10 -0

K/?,G,H2S 4 K/A,G,H2S 2

+2 -4

+6 -0

+0 -6

+6 -0

(1) GNB, Gram-negative bacillus; GPB, Gram-positive bacillus

(2) Carbohydrate fermentation results shown as acid/gas

(3) TSI slant/butt - A, acid; K, alkaline; G, gas production, H2S, hydrogen sulfide production