Need very soon

BIO2073: Microbiology Lab

Individual Assignment

Biochemical Tests: Phenol Red Fermentation Broth and Catalase Test

1. For the Phenol Red Fermentation Broth, what two key ingredients allow us to test for fermentation? Explain the purpose of each.

2. How can a color change of the broth from red to yellow tell us that fermentation has occurred?

3. What is a Durham tube? Why might we include it in our PR test broth?

4. Write out the equation for the reaction that is occurring during the catalase test.

5. In the catalase test, what is causing the formation of bubbles that we interpret as a positive reaction? (In other words, what is being released during the reaction?)

Bonus! If you tested an organism using the Phenol Red glucose test, and it was positive, could you accurately predict that your organism would also test positive using sucrose? Why or why not?