microbiology

Ori p lac p lac

lac Z

lac I

lac Y

lac A

Use Restriction Endonuclease lac Z

lac I

lac Y

lac A

Ori Ori

p lac

mlr A

mlr B

mlr C

mlr Dlac I

mlr A mlr B

mlr C mlr D

Use PCR mlr A

mlr B

mlr C

mlr D Sphingomonas sp. (strain ACM-3962)

chromosomal DNA

Liga tion

Insert Into E. coli

Ori p lac

mlr A mlr B mlr C

mlr D lac I

Plate On LB Agar

Cyanobacteria (Microcystin- Lr)

Microcystic-LR + Transformed E. coli

Transformed E. coli

(Temperatures 0°C, 10°C, 20°C, 30°C, 40°C)

E. coli

30 °C

10 °C0 °C

40 °C

20 °C

(control group)

*Cyanobacteria grows in higher temperature*

If there’s no growth, then new genes are expressed

Biodegradation Pathway for Microcystins (Hydrolyses)

Plate on LB Agar *ONLY plate Transformed E. coli*

(Temperatures 0°C, 10°C, 20°C, 30°C, 40°C) 10 °C 20 °C

30 °C 40 °C

Insert Into Cow Feed

Ori p lac

mlr A mlr B mlr C

mlr D lac I

Feed To Cow

Boo D

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