Lab reports

Aseptic Technique Lab Report 1

Note from student: our assignment is to look at a visual lab that was given to us by our professor and then write a lab report of what we saw in the visual lab.

Important: There are two labs report at the bottom can you please rewrite the same lab reports in your own words without plagerizing the original lab report. This lab report is my friends.

1 Aseptical technique lab report.

Introduction

This lab involved learning the vast nature of bacteria. This was done through putting the sterile media through various environments. This lab gives the opportunity to practice the many techniques used to help prevent microbial culture contamination with unwanted bacteria that was in the surrounding environment. In the case study, a laboratory technician named Jeanne. In this, she was working at a clinical microbiology facility for testing. The testing dealt with working with various cultures and the testing of a patient specimen in search of the right ID for microbes that were infected. After weeks, Jeanne received the sample from the case suspected of bacterial meningitides. Her tests concluded and demonstrated that the patient was infected with meningococcal meningitis. Jeanne was infected less than a week later. She was then placed in the hospital due to her vomiting, high fever along with headaches and she was constantly in a state of confusion. While she was in the hospital, she had been tested for meningococcal meningitis and the tests came back positive diagnosing her with it.

Materials and Methods

This lab consisted of investigating bacterial presence on items we potentially use every day. This being done by seeing how washing your hands affects good and bad bacterial quantity. The materials that were given were a soda can, banana, money, sharpie, and a keyboard. You collect bacteria from the different samples and grow them in a petri dish. Steps included:

1.) Place a sterile plate on the lab table and label it.

2.) Dip the cotton tip in the bacterial medium tub.

3.) Swab the environment and streak the petri dish.

4.) Get all petri dishes ready for incubation.

5.) Incubate all petri dishes.

6.) Observe the petri dishes after incubation has occurred.

Growth that will be observed will be orange spots which are called colonies that are on the surface of the agar plates. Keep in mind, change the cotton swab for each time you go to swap another sample. This is to help prevent cross contamination and provide accurate results. In addition to this lab, I had to discover ethanol impacted the disinfection of a lab work space. With this, you learn how important it is to be knowledgeable on the ubiquity of bacteria also with what environmental factors can help influence bacteria growth.

Results

In this lab, I learned how to apply my knowledge on how to analyze microbial growth that forms in various environments. While looking through the microscope at the colonies, I learned how to pick which exposure plates had the greatest diversity of microbes. The prime time to observe incubated samples is between 24 and 48 hours. Some samples may even surprise you with the amount of bacteria on the substance you tested. You learn to use colony numbers to estimate conclusions about the different samples used in the experiment. In regards to the bacterial disinfection part of this lab, you learn how important it is to disinfect your lab space and how much contaminants are on your lab space.

Discussion and Conclusion

I enjoyed doing this lab. I learned a lot about how bacteria is everywhere but that some of that bacteria is good bacteria. I think I had the most fun with discovering how much bacteria was on the everyday objects we use. I feel like I learned the necessary things from this experiment about bacteria surfaces. Also, I learned the importance of cleaning the lab surface because there are a lot of bacteria in the air of the lab. Overall, this a lab I would do again.

Works Cited:

M. (n.d.). Aseptic Technique. Retrieved April 01, 2018, from http://www.mhlearnsmart.com/flow/binrunner.html?pixi=0&isbn=1259337782&title=&deck_id=&learnsmart_id=12981612&learnsmarturl=www.mhlearnsmart.com&parisId=26183570&instructorParisId=6418639§ion_id=99815457&assignment_id=200100794&newals=0&paamskin=&first_name=Dylan&last_name=Todd&[email protected]&password=&type=S&salt=321740177&key=12bfcb506f303430eb8e33800a2f3ee2&assignmentDueDate=2018-04-02 03:59:00&studentExtensionDate=&isBlackBoardUrl=false&ebookLinkUrl=http://connect.mheducation.com/connect/hmEBook.do§ionURL=http://connect.mheducation.com/connect/hmStudentSectionHomePortal.do?sectionId=99815457§ionName=18SPCMP BIO-299 5&libraryURL=&preview=&plan=0&externalProductId=A9S0004102

2 Microscopy microbiology lab report

Introduction

Microorganisms are too small for the naked eye for one to see so we use a bright field microscope. A bright field microscope is a microscope that uses a light to show the microorganisms, hence the name bright field. Bright field microscopes can identify many microorganisms like bacteria, algae’s, Achaea, fungi, and so one. In the lab that one will conduct will be able to know how operate a bright field microscope and be able to use it to identify a bacteria a patient has contracted by using multiple steps. Not only that but one will learn, comprehend, and know some different kinds of eukaryotic cells, bacteria, algae, and some other microorganisms. One also must be able to identify the different type microorganism through a bright field microscope by the knowledge one has obtained from learning them previously.

Materials and Methods

The experiment for this lab involves identifying and comprehending different microorganisms. With the knowledge that one has obtain from the microorganisms can identify a sample under a bright field microscope. The materials needed for this lab is listed below:

· Bright field microscope

· Different types of microorganisms that are given

· Glass slides and covers

· Pipette

· Tweezers

· Bacteria sample from the patient

Before beginning the experiment, one must be educated on how to use a bright field microscope as well as the differences between the different microorganisms that are being used in this experiment. One must go through the different modules learning about the different microorganism and the using bright field microscope to view them as well as using the microscope in the simulator. After doing these important steps then one can move on to conduct the experiment to figure out the patient’s diagnosis by looking at the vaginal sample. To be able to view the sample, one must use the microscope to decide the patient’s diagnoses.

Results

There are three possible diagnoses: vaginal candidiasis, trichomoniasis, and Bacterial infection. Vaginal candidiasis also known as a yeast infection shows the budding of yeast cells and pseudohphae interspersed among squamous epithelial cells. Trichomoniasis is a number of flagellated protozoan cells that are clustered around a squamous epithelial cell. The third is bacterial infection, which have “clue cells” that are squamous epithelial cells that are covered with rods shaped bacteria and are indicative of a condition; this is caused by gardnerlla vaginalis. After looking at the sample through a bright field microscope one can diagnosis the patient with vaginal candidiasis or yeast infection as most people call it. Antibiotics’ disrupting normal microbiota providing an opportunity for yeast to overgrow causes it. To treat a yeast infection, the patient must get a prescription for a antifungal drug following with a antibiotic treatment depending how the doctor wants to treat the her. A way to prevent this is by eating lot of probiotic yogurt because probiotic yogurt contains active cultures of “friendly” bacteria, which can help restore the normal balance of bacteria.

Conclusion

In conclusion, this lab taught oneself the necessary actions and knowledge of a bright field microscope to be able to look at samples of microorganisms and identifying them. Not only that but to know the differences of the microorganisms tested in this experiment along with how they look under a microscope. With all of this knowledge one was able to diagnosis the patient with yeast infection, which is a fungi that can be treated with antifungal and antibiotics.

Works Cited

Cowan, Marjorie K. (2015). Microbiology: A Systems Approach. (4th ed.). New York: NY: McGraw-Hill.

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