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AntibioticSensitivityLabReportOL.docxBIO2073- Microbiology Lab
Antibiotic Sensitivity Testing – Group Activity
1. What is the objective of this week’s laboratory exercise?
2. This table was completed using the information from the data file. For each plate, one sample remains to be measured. A ruler is on the slide so that you can measure the zone of inhibition. Use the Interpretive Standards handout to determine the level of resistance: Resistant (R), Intermediate (I), or Sensitive (S).
|
Antibiotic |
Antibiotic Code |
Escherichia coli |
Proteus mirabilis |
Staphylococcus aureus |
|||
|
|
Zone of inhibition (mm) |
Susceptible/ resistant/ intermediate |
Zone of inhibition (mm) |
Susceptible/ resistant/ intermediate |
Zone of inhibition (mm) |
Susceptible/ resistant/ intermediate |
|
|
Bacitracin |
B10 |
6 |
|
6 |
|
6 |
|
|
Penicillin |
P10 |
6 |
|
|
|
6 |
|
|
Chloramphenicol |
C30 |
27 |
|
20 |
|
23 |
|
|
Neomycin |
N30 |
19 |
|
20 |
|
|
|
|
Erythromycin |
E15 |
10 |
|
6 |
|
25 |
|
|
Tetracycline |
Te30 |
15 |
|
6 |
|
22 |
|
|
Streptomycin |
S10 |
15 |
|
16 |
|
14 |
|
|
Sulfamethoxazole + Trimethoprim |
SXT |
|
|
22 |
|
23 |
|
3. Based on the results, which antibiotics might be useful against the strain of Proteus mirabilis tested here?
4. The paper discs are labeled with abbreviations (e.g., B10). The letter refers to the name of the antibiotic. What does the number refer to?
