Biochemistry lab reports writing

t:lvn~;.. 'if IViE lRiGLYC ER ID ES KIT Foe lhe dete\m~~~ !, ~!;:~!!;~?,~~ o; plasma ~

Summary (For lnvitro Diagnosti c Use Only) ~ )

Triglycerides are a form off tt ·d \ and other fatty a ·d Th a Y ac, esters. They are produced in the liver by binding glycerol energy I cid sl. ey are tran sported by VLDL and LDL and act as a storage source for . ncrease evels are found, ·n hyp 1 - ·d . . h h • . er 1p1 em,as diabetes nephrotic syndrome ; ~~ot yroidism · Increased levels are ri sk factor for arte(iosclerotic' co ronary disease and / P lphera l vascular disease . Decreased levels are fo und in malnutrition and hyperthyroidism.

Prin ciple Lipoprotein lipase hydrolyses triglycerides to glycerol and free fa tty acids . The glycerol fo~~ ed with ATP in the presence of glycerol kinase form s glycerol 3 phosphate which is 1 ox1d1sed by the enzyme glyceroi phosphate oxidase to fo rm hydrogen peroxide. The hydrogen peroxide further re acts with phenolic compound and 4-aminoantipyrine by the catalytic action of peroxidase to fonn a red coioured qu i;,or. eir nir, e dye complex . Intensity of • the colou r form ed is directly proportional to the amount of triglycerides present in the sample.

Upoprotein Lipase Tri glycerid es

Glycerol Kinase Glycerol+ ATP

Glycerol 3 PO Gfycerof 3 Ph op hate+ O,

H,O, +4Aminoa ntipyrin e + Phenol ·

Peroxidase

Glycerol + Free fatty acids

Glyce rol 3 Phophate + ADP

Dihyd roxy acetone phos. + H,0,

Red Quinoneimine dye+ H,0

.~(i,11iai rnferencb ,.-a!uei:: Serum/ Plasma ( Suspicious) 150 rrg/d' and dbo,<'!

( El evated) 200 mg/di and above It Is recommended th at each laboratO') establ1sn its own normal range representing patient population.

Contents 75 ml 2 x 75 ml L 1 : Enzyme Reage nt 1 60 ml 2 x 60 ml L2 : Enzyme Reagent 2 15 ml 2 x 15 ml S : Triglycerides Standard (200 mg/di) 5 ml 5ml

Storage / stability

Contents are stable at 2-8°C ti ll the expiry mentioned on the labels .

Reagent Preparation Reagents are re ady to use . Working reagent : Pour the contents of 1 bottle of L2 (Enzyme Reagent 2) into 1 bottle of L 1 (Enzyme Reagent 1 ). This working reagent is sta ble for at least 8 weeks when stored at 2-8' C. Upon storage the working reagent may develop a slight pink colour however this does not affect the performance of th e reagent. Alternatively fo r fl exibility as much of work ing reagent may be made as and when desired by mixing together 4 parts al 11 (Enzyme Reagent 1) & 1Jart of L2 ( Enryme Reagen\2 ). Alternatively Q~ ml of L 1 and 0.2 ml of L2 may also be used instea d of 1 ml of the wor mg reagent directly during the assay.

Sample material ~;u~lasma. Triglycerides is reported to be stable in the sample for 5 days when stored at <<:J

EME

-- -

1 / . / V \

' "" l c " c.1, .... - · - ,. c.a" ,i. 1Pst I• •be.- -b d Bl B -~ - · - u - - ., 1 - ,., -~ ci e,e as anrt 1 .;,ta·,dard S

1 , andTest(T) I - - ------

Ad drti ori B S T Seque nce

I (Ti I 1m, (ry, l

.Von<"ng Reagent JJl- 1.0 D st ed ... ater ~ -r ~ ;cemfos Stanaara < S )

0.01 Sample - 1.0

Jl_Ul

W ,. M:: 1 a i1d incuc,ate at 3"fC fo r 5 'l1 n. or a1 R. T f25' C) for 15 rri in. Measure the absorbance o f r.,e S'.and ar':! /.bs S ard Test Samp1e (Aos T) against the Blank, within 60 Min.

Calculations

Tr g!-ycences Il -ng c =

D-f11ct f ?-i:Y 17

Abs .T ~ X 200

Abs .S

Linearity - .. s ;;roceo..re s ' rear upto 1000 mg/di If -1alues exceed trns limit, dilute the serum wrth ..,- norma sa ne ,r-,laS S S%, ar,d re~at the;,~y

t- Note ::: as r g 53,,..::; es cf .. 2 1.0 i ,1 r ·s are preferred Fatty meals and alcohol may cause e'e ,ated •esu ts Pa~er• s"'cJid '10t i:lrrl<. alconot fer 24 hrs 0€fore the test

r- -- --

Reaction

Wavelength

Zero Setting

lncub. Temp.

lncub. Time

Delay Time

Read Time

No. of read.

System Parameters

: End Port Interval : 505 nrr Sample Vol r1 ': rr l : Pe3gen1 8 1ari), Reagent Vol , '/J "

: '1I'C IP T Standard . t 'YJ IT'fJ ''J I : 5 mt, .. I '5 m1r Factor

- React Slope ri:rea-; rig Linearity 10'"J(J mg/di

Units mg/<JI

EUROMED I EQU IP LTD. 46 , WE IBECK RO.AD , V/ EST HA RPO/,

MIDD/ , HA2 ORV/ , UK