Complete the Biology Labs
JenkinsL12
3.IdentificationofCarbohydratesProteinsandLipids.docxLab 3 Identification of Carbohydrates, Proteins, and Lipids Assignment
Results
1. While looking at the pictures for the results of each test, fill in the chart below (+/- means was the color positive or negative):
|
No.
|
Substance |
Benedict Test |
Iodine Test |
Biuret Test |
Sudan Dye |
|||
|
|
|
color |
+/- |
color |
+/- |
color |
+/- |
+/- |
|
1 |
water (control) |
|
|
|
|
|
|
|
|
2 |
10 % glucose |
|
|
|
|
|
|
|
|
3 |
10 % sucrose |
|
|
|
|
|
|
|
|
4 |
10 % starch |
|
|
|
|
|
|
|
|
5 |
10 % albumin |
|
|
|
|
|
|
|
|
6 |
salad oil |
|
|
|
|
|
|
|
|
7 |
milk |
|
|
|
|
|
|
|
|
8 |
regular soda |
|
|
|
|
|
|
|
|
9 |
unknown |
|
|
|
|
|
|
|
2. Fill in the table below for the results of the Benedict Test (look at the color for each test tube and then match up the color in the table found on page 7):
|
No. |
Substance |
Gm % |
|
1 |
water (control) |
|
|
2 |
10 % glucose |
|
|
3 |
10 % sucrose |
|
|
4 |
10 % starch |
|
|
5 |
10 % albumin |
|
|
6 |
salad oil |
|
|
7 |
milk |
|
|
8 |
regular soda |
|
|
9 |
unknown |
|
Answer questions with complete sentences.
3. A clear regular (non-diet) soda was tested. How much reducing sugar was present in the sample? Is the reason it is sweet because it contains reducing sugars? How do you know this?
4. Did the milk you tested contain protein? How do you know this? If it contained protein can you determine how much? Why or why not?
5. Do you think the Sudan Dye Test is a quantitative or qualitative test? Why or why not?
6. What is an indicator substance? Give an example.
7. Which tests gave you a positive result when you tested your unknown? What does this tell you?
8. Outline the functions of the polysaccharides in the table below.
|
Polysaccharide |
Function |
|
Glycogen |
|
|
Chitin |
|
|
Cellulose |
|
|
Starch |
|
9. Outline the functions of the proteins in the table below:
|
Protein |
Function |
|
Hemoglobin |
|
|
Keratin |
|
|
Myoglobin |
|
|
Fibrin |
|
|
Serum albumin |
|
|
Insulin |
|
|
Calmodulin |
|
|
Immunoglobins |
|
|
Cytochromes |
|
|
Vasopressin |
|
|
Pepsin |
|
|
Ferritin |
|
10. Outline the functions of the lipids in the table below:
|
Lipid |
Function |
|
Lecithin |
|
|
Prostaglandins |
|
|
Cholesterol |
|
|
Estrogen |
|
|
Carotene |
|
11. Which groups of organic molecules would you expect to find in the following:
a. corn oil –
b. silk –
c. rubber –
d. soap –
e. molasses –
Lab 3
I
dentification of
C
arbohydrates
, P
roteins
,
and
L
ipids
Assignment
Results
1.
While looking at the
pictures for the
results of each test, fill in the chart below
(+/
-
means
was the color positive or negative)
:
No.
Substance
Benedict
Test
Iodine Test
Biuret
Test
Sudan
Dye
color
+/
-
color
+/
-
color
+/
-
+/
-
1
water (control)
2
10 %
glucose
3
10 % sucrose
4
10 % starch
5
10 % albumin
6
salad oil
7
milk
8
regular soda
9
unknown
2.
Fill
in
the table below for the results of the
B
enedict
T
est
(look at the color for each test tube
and
then match up the color in the table found on page 7)
:
No.
Substance
Gm %
1
water (control)
2
10 % glucose
3
10 % sucrose
4
10 % starch
5
10 % albumin
6
salad oil
7
milk
8
regular soda
9
unknown
Answer questions with complete sentences.
3.
A
clear regular (non
-
diet) soda
was tested
. How much reducing sugar was present in
the
sample? Is the reason it is sweet because it contains reducing sugars? How do you know
this?
Lab 3 Identification of Carbohydrates, Proteins, and Lipids Assignment
Results
1. While looking at the pictures for the results of each test, fill in the chart below (+/- means
was the color positive or negative):
No.
Substance
Benedict
Test
Iodine Test Biuret
Test
Sudan
Dye
color +/- color +/- color +/- +/-
1 water (control)
2 10 % glucose
3 10 % sucrose
4 10 % starch
5 10 % albumin
6 salad oil
7 milk
8 regular soda
9 unknown
2. Fill in the table below for the results of the Benedict Test (look at the color for each test tube
and then match up the color in the table found on page 7):
No. Substance Gm %
1 water (control)
2 10 % glucose
3 10 % sucrose
4 10 % starch
5 10 % albumin
6 salad oil
7 milk
8 regular soda
9 unknown
Answer questions with complete sentences.
3. A clear regular (non-diet) soda was tested. How much reducing sugar was present in the
sample? Is the reason it is sweet because it contains reducing sugars? How do you know
this?
