Microbiology lab 2

2420-Lab 9- Immunology- Agglutination

Directions:

Answer questions after reading the information and watching the video from the links below. Use color RED or BLUE for your answers. Submit the completed document on eCampus for grading. Refer to

· the textbook chapters 15, 18 (sections 15.4, 18.3, 18.4), (Nester- McGraw Hill)

· Link: Agglutination- (Antigen-Antibody reaction) - I (6-A, B,) (Virtual Edge Experiment-6- Introduction, A –skip B)

I. Introduction:

Read from

· the textbook chapters 15, 18 (sections 15.4, 18.3, 18.4), (Nester- McGraw Hill)

· Link: Agglutination- (Antigen-Antibody reaction) - I (6-A) (Virtual Edge Experiment-6- Introduction, A)

Define the following terms:

1. Antigen

2. Antibody

3. Epitope

4. Immunoassay

5. Polyclonal antibodies

6. Monoclonal antibodies

7. Agglutination

8. Precipitation

II. Hemagglutination (Blood typing)

For background: 6-A

9. Complete the following table.

Blood type	Antigens present On the erythrocyte	Antibodies present in plasma	Can donate blood to	Can receive blood from
A+
A-
B+
B-
AB+
AB-
O+
O-s

10. Draw the results of different blood types in the following trays

Blood type	Hemagglutination results Well A-with anti-A antibodies	Hemagglutination results Well B- with anti-B antibodies	Hemagglutination results Well Rh- with anti-Rh antibodies
A+
A-
B+
B-
AB+
AB-
O+
O-