Lab assignment #1

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2020SU-BIOL2420-85201Adjuted.docx

RICHLAND COLLEGE, Department of Biology,

School of Mathematics, Science & Health Professions

Microbiology syllabus for on-science majors

Instructor Information

Name: Admassu Mitiku

Email: [email protected]

Office Phone: 972-238-6140

Course Information

Course title: Microbiology for Non-Science Majors

Course number: Biol 2420

Section number: 85201

Semester/Year: Summer 2020

Credit hour: 4

Online meeting times

Monday to Friday: Mornings until noon. Meet via college email

Saturday/Sunday: 5:00 8:00pm. Meet via college email

Important dates

Certification Date: 08/06/2020

Drop Date: 07/16/2020

Final exam: Tuesday, August 4, 2020

General course information

Prerequisite:

BIOL 1406 or BIOL 2401 or SCIT 1407. One of the following must be met: Student cannot take both BIOL 2420 and BIOL 2421 to satisfy the Core science credit.

Course Description:

Study of the morphology, physiology, and taxonomy of representative groups of pathogenic and nonpathogenic microorganisms. Emphasis is placed on applications to humans. Pure cultures of microorganisms grown on selected media are used in learning laboratory techniques. Includes a brief preview of food microbes, public health, and immunology. Designed for non-science majors and allied health students. (3 Lecture, 4 Lab.)

Student learning outcomes:

Upon successful completion of this online course lecture and lab parts, students will:

1. Describe distinctive characteristics and diverse growth requirements of prokaryotic organisms compared to eukaryotic organisms.

2. Provide examples of the impact of microorganisms on agriculture, environment, ecosystem, energy, and human health, including biofilms.

3. Distinguish between mechanisms of physical and chemical agents to control microbial populations.

4. Explain the unique characteristics of bacterial metabolism and bacterial genetics.

5. Describe evidence for the evolution of cells, organelles, and major metabolic pathways from early prokaryotes and how phylogenetic trees reflect evolutionary relationships.

6. Compare characteristics and replication of acellular infectious agents (viruses and prions) with characteristics and reproduction of cellular infectious agents (prokaryotes and eukaryotes).

7. Describe functions of host defenses and the immune system in combating infectious diseases and explain how immunizations protect against specific diseases.

8. Explain transmission and virulence mechanisms of cellular and acellular infectious agents.

Upon successful completion of this course lab part, students will:

1. Use and comply with laboratory safety rules, procedures, and universal precautions.

2. Demonstrate proficient use of a compound light microscope.

3. Describe and prepare widely used stains and wet mounts, and discuss their significance in identification of microorganisms.

4. Perform basic microbiology procedures using aseptic techniques for transfer, isolation and observation of commonly encountered, clinically significant bacteria.

5. Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

6. Perform basic bacterial identification procedures using biochemical tests.

7. Estimate the number of microorganisms in a sample using methods such as direct counts, viable plate counts, or spectrophotometric measurements.

8. Demonstrate basic identification protocols based on microscopic morphology of some common fungi and parasites.

Texas core course objectives: Students will be able to describe the morphology, physiology, and taxonomy of representative groups of pathogenic and non-pathogenic organisms, and apply techniques used in growing pure cultures as it relates to humans and public health issues.

Required course materials:

A. Text book: Microbiology with Diseases by Taxonomy, 6thedition by Robert W. Bauman.

B. Mastering Microbiology: Three options to buy required course materials for Mastering Microbiology online work/assignments:

1. Print Textbook + etext + mastering code ISBN: 9780135159927

2. Books a la carte + etext + mastering code ISBN: 9780135204337

3. Mastering code alone + eText (no book) ISBN: 9780135174722

Please make sure that the code you purchase (either from a book store or online) matches the textbook, "Microbiology with Diseases by Taxonomy 6th edition" and NOTMicrobiology with Diseases by Body Systems". Please be advised that access code is mandatory for this course.

A lab manual is available online at this link: https://web.archive.org/web/20190113211746/http://delrio.dcccd.edu/jreynolds/microbiology/RLCmicroindex.html Link also has other resources, such as: hand-outs on safety in microbiology lab, practice questions for lab quizzes and practical exams, graphics/images and videos.

You need to check manual from this link. Link also includes course materials such as, lab practical graphics, practice questions for lab practical exams that go along with the lab manual, Lab. safety handouts and video links for lab procedures.

e-Campus - http://ecampus.dcccd.edu – Please visit this site as often as possible for course materials: Syllabus, PowerPoint lecture notes, study guides for lab quizzes/lecture tests, lab assignments, videos/audios, guide lines for Unknown ID report writing, lab assignments and grades etc. are all posted on e-Campus.

Institutional Policies

Institutional Policies relating to this course can be accessed from the following link: www.richlandcollege.edu/syllabipolicies

Other course policies

Attendance: Attendance is necessary for class/lab participation and course work. There will be no make-up opportunities if a student misses lab practical exams or lecture exams. However, there could be make-up for missed quizzes, tests and assignments if a student can present concrete evidence (example: medical reasons, etc.). Student should contact instructor in advance and at a reasonable time and submit evidence for absence for quiz/test and assignment re-sets.

Plagiarism/cheating: Plagiarism, defined as deliberate use of someone else’s language, ideas, or other original (not common-knowledge) material without acknowledging its source. Plagiarism is not allowed in any online assignments or home works. Cheating in this course, in any manner and circumstance is not allowed. Any student violating any of the above rule(s) will get a ZERO.

Grading and grading scales

Students may earn a maximum of 1000 points for the lecture, lab components and individual/group assignments combined. Table below lists the details of lecture and lab components and point distributions. In addition, a maximum of 50 extra credit points are allowed to count on top of the total grade (1000pts). 

Break down of grade components and grading scale for letter grade are assigned as follows:

Course components Grade points

1 Final Exam (Comprehensive) 100 3 Lecture tests (100 pts each) 300 Online Mastering quizzes 100

Practical exam # 1 100

Practical exam # 2 50

3 Lab quizzes (50 pts each) 150

1 Lab assignment 50

Unknown ID (Enteric bacteria) 100

Unknown ID (Staph/Strep) 50

----------------- Total 1000

Grading Scale: Final letter grades are determined following standard procedure (standard grading scale) as follows: 900 - 1000 = A; 800 - 899 = B; 700 - 799 = C; 600 - 699 = D; less than 600 = F

Lab schedule for Biol-2420-85201 Summer 2020

Biology 2421 Microbiology for Science Majors Summer 2020

Week and Unit

Reading assigned in Microbiology text

Lab

Graded assignment

Due date

WEEK 1: short week starts 6/4

Ch1- Introduction to history of Microbiology

Ch2- Microbial chemistry

-Aseptic transfer of bacteria

-Pure culture techniques

-Microscopy use and preparation of specimens

WEEK 2:

Starts 6/8

Ch3- Microbial structures and function

Ch4- Microscopy and staining

-Gram Stain

-Endo-spore stain,

-Acid-fast stain (AFS)

-Capsule Stain

-Flagella stain

-Motility and motility tests

WEEK 3:

Starts 6/15

Lecture exam # 1 (chapters; 1-4)

Ch5- Microbial metabolism

Ch6- Microbial nutrition and growth

July 16 – LAST DAY TO WITHDRAW

-Colony Morphology

-Dilutions & Pipetting

-Counting Bacteria

-Environmental Conditions & Growth

-Effects of Temperature

-Protozoa

-Fungi

Lab assignment (Pipetting and /dilutions) – 50 pts

06/18/20

Lec. Test # 1 opens on Monday, June 15 @10:00am and closes on Monday, June 15 @midnight

WEEK 4:

Starts 6/22

Ch7- Microbial genetics

Ch8- Recombinant DNA technology

Lab quiz # 1

-Antibiotic (Kirby-Bauer) Sensitivity

-Antimicrobial Chemicals

-Ecto-parasites

-Helminths

Lab quiz # 1 opens on Monday June 22 @10:00am and closes on Monday, June 22 24 @midnight

WEEK 5:

Starts 6/29

Ch9- Control of microbial growth in the environment

Ch10 –Control of microbial growth in the human

Lecture exam # 2 (chapters: 5-8)

Practical exam # 1

Unknown (Enteric bacteria) ID

-Oxygen Requirements

-Biochemical tests: IMViC, TTC, Phenol Red broth, Oxidase, Catalase, Nitrate, Decarboxylase, Deaminase, Gelatin, Skim Milk, Lipid, Starch, Urea

.

Practical exam # 1 opens on Monday, June 29 @10:00m and closes on Monday, June 29@midnight

Lec. Test # 2 opens on Monday, July 6 @10:am and closes on Monday, July 6 @midnight

WEEK 6:

Starts 7/6

Ch13- Viruses and viroids

Ch14- Infection and infectious disease

-API 20E identification

-Complete enteric bacteria unknown ID

WEEK 7:

Starts 7/13

Lecture exam # 3 (chapters: 9, 10, 13 and 14)

Lab quiz # 2

-Staphylococci unknown ID

-Serological Testing

-Complete Staph unknown and submit report

Lec. Test # 3 opens on Friday, July 17 @10:00am and closes on Friday, July 17 @midnight

WEEK 8:

Starts 7/20

Ch17- Vaccines and immunization

-Streptococci unknown ID

-Serological Testing

-Complete Strep unknown and submit report

-Bacteriophages

-Urine culture

Lab quiz # 3

Essay (Extra credit) – 50 pts

Saturday July 24@midnight

Lab quiz # 3 opens on Saturday, July 25 @10:00am and closes on Saturday, July 25 @midnight.

WEEK 9:

Starts 7/27

Practical exam # 2

Practical exam opens on Friday, July 31 @10:00am and closes on Friday, July 31 @midnight

WEEK 10:

Starts 8/3

Final exam (Comprehensive) – Exam opens on Tuesday, August 4 @10:00am and closes on Tuesday, August 4 @midnight

Disclaimer: The instructor reserves the right to amend syllabus, course contents, grading procedures, and/or other related items as conditions dictate. Students will be notified of any changes that are to be made in advance via email (or ecampus announcement).

1

1

RICHLAND COLLEGE,

Department of Biology

,

School of Mathematics, Science & Health Professions

Microbiology syllabus for on

-

science majors

Instructor Information

Name: Admassu Mitiku

Email:

[email protected]

Office Phone:

972

-

238

-

6140

Course Information

Course title: Microbiology for Non

-

Science Majors

Course number: B

iol

2420

Section number: 852

0

1

Semester/Year:

Summer

2020

Credit hour: 4

Online m

eeting times

Monday to Friday:

Mornings until noon. Meet

via

college email

Saturday/Sunday: 5:00 8:00pm. Meet

via

college email

Important dates

Certification Date:

08/06/2020

Drop Date:

07/16/2020

Final exam:

Tuesday

, August

4

, 2020

G

eneral course information

Prerequisite:

BIOL 1406 or BIOL 2401 or SCIT 1407.

One of the following must be met: Student cannot take both

BIOL 2420 and BIOL 2421 to satisfy the Core science credit.

Course Description:

Study of the morphology, physiology, and taxonomy of representative groups of pathogenic and

nonpathogenic microorg

anisms. Emphasis is placed on applications to humans. Pure cultures of

microorganisms grown on selected media are used in learning laboratory techniques. Includes a brief

preview of food microbes, public health, and immunology. Designed for non

-

science maj

ors and allied

health students. (3 Lecture, 4 Lab.)

1

RICHLAND COLLEGE, Department of Biology,

School of Mathematics, Science & Health Professions

Microbiology syllabus for on-science majors

Instructor Information

Name: Admassu Mitiku

Email: [email protected]

Office Phone: 972-238-6140

Course Information

Course title: Microbiology for Non-Science Majors

Course number: Biol 2420

Section number: 85201

Semester/Year: Summer 2020

Credit hour: 4

Online meeting times

Monday to Friday: Mornings until noon. Meet via college email

Saturday/Sunday: 5:00 8:00pm. Meet via college email

Important dates

Certification Date: 08/06/2020

Drop Date: 07/16/2020

Final exam: Tuesday, August 4, 2020

General course information

Prerequisite:

BIOL 1406 or BIOL 2401 or SCIT 1407. One of the following must be met: Student cannot take both

BIOL 2420 and BIOL 2421 to satisfy the Core science credit.

Course Description:

Study of the morphology, physiology, and taxonomy of representative groups of pathogenic and

nonpathogenic microorganisms. Emphasis is placed on applications to humans. Pure cultures of

microorganisms grown on selected media are used in learning laboratory techniques. Includes a brief

preview of food microbes, public health, and immunology. Designed for non-science majors and allied

health students. (3 Lecture, 4 Lab.)