Advanced Biochemistry - Metabolism 45 questions assignment

Respira(on Addi(onal Slides

Strategies for Energy Conserva(on

Brock Biology of Microorganisms, Madigan et al. Pearson Publishers, 15th edi(on

•  “The obscure we see eventually, the completely apparent takes longer”.

•  Peter Mitchell

1958 •  Respira(on involved O2-dependent oxida(on of metabolically-produced NADH

•  Membranes were required •  The mitochondrion was the site of respira(on in animal cells

•  The chloropoast was the site of ATP synthesis in plants

•  In Fermenta(on, ATP synthesis required a “high energy intermediate” to transfer PO4, making ATP (eg, Acetyl Phosphate)

circa 1960 (Peter Mitchell Nobel Address)

Chemical Coupling Hypothesis circa 1960

(Peter Mitchell Nobel Address)

Keilin’s Respiratory chain circa 1953

(Peter Mitchell Nobel Address)

Mitchell’s Hypothesis Linking ion transport, respira(on

circa 1960 (Peter Mitchell Nobel Address)

General Scheme for Fermenta(on

Glyceraldehyde-3-phosphatee dehydrogenase Phosphoglycerate Kinase

An example of substrate level phosphoryla(on

Electron Transport in Bacteria

Coenzyme Q – the Quinone H and e- Carrier in electron transport

Iron-Sulfur Centers

Electron Donors & Receptors in metabolism

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