Descriptive Questions and answers

1.For each of the 4 microscopes below, list some items that you would be able to view with that particular type of microscope.
Compound Light:
SEM:
Dissecting Scope:
TEM:

5. Define the following:
a. magnification
b. resolution
c. contrast
d. ocular micrometer

6. Complete the following table:
Objective magnification Ocular magnification Total magnification
10X 10X
45X 10X
100X 10X
10X 750X
45X 225X
7. While looking through the 40X objective lens, you use the micrometer and measure an insect’s leg. It is 16 mm. What is the actual size of the insect leg?
8. Explain why the light microscope is also called the compound microscope.
9. Explain why the specimen must be centered in the field of view on low power before going to high power.
10. Describe how to make a proper wet mount of the letter e.

11. Does low power or high power have a larger field of view and allow you to see more of the object?
12. List the steps involved in the gram staining procedure in the table below:
Name of Step--- Name of Reagent----- Time Color of Gram + Color of Gram -
Rinse water
Rinse water
Rinse water
13. How does the gram stain differentiate between the 2 strains of bacteria?
14. Why is the gram stain important? What can it tell you? What can you do with the information?
15. How would a doctor treat gram positive bacteria versus gram negative bacteria?
16. Name 3 bacteria and provide the following:
Scientific Name of Bacterium -- Gram Result -- Morphology (shape)-- Disease Produced