esolutions
Extractionof DNA
Hands-On Labs, Inc. Version 42-0056-00-01
Lab Report Assistant
Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables thatshouldbeaddressedinaformallabreport.Theintentistofacilitatestudents’writingoflab reports by providing this informationin an editable filewhichcan be sent to an instructor.
Questions
A. Describe what the DNA from the split peas looked like?
B. Wereyourexperimentalresultswhatyouexpectedtosee?Specifically,wastheremoreor less DNA than you expected?
C. Whatwasthepurposeofaddingthedetergenttotheexperiment?Ifthedetergentwasnot used, do you think the experiment results would have changed? Explain your answer.
D. Ifyouused5%isopropylalcoholinsteadof91%isopropylalcohol,doyouthinktheexperiment results would have changed? Explain your reasoning.
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Mitosis
Hands-OnLabs,Inc. Version42-0091-00-01
LabReport Assistant Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables thatshouldbeaddressedinaformallabreport.Theintentistofacilitatestudents’writingoflab reports by providing this informationin an editable filewhichcan be sent to an instructor.
MitosisWorksheet- Answer the questionsabout the photos below:
Onion root tipphotos at 600x and 1000x taken by Stan Carpenter of Hands-On Labs, Inc.
1. What phase is this cell undergoing?
2. What happens afterthis phase?
1. The two center side-by-side cells are undergoingtwodifferent phasesof mitosis.
a. What are these phases?
b. Whatdiagnosticfeaturescanhelp you with your decision?
1. What phase is this cell undergoing?
2. How can you tell?
1. This cell has clearly visible chromatids.
a. What'sthenextphasethiscellwill undergo?
b. Wherewillthechromatidsgointhe coming phase?
1. Thecellinthelowercenterisexhibitingsigns of what phase of mitosis?
2. Whatphasedoesitappearthecellaboveis undergoing?
1. Explainwhathappensduringanaphase.
2. Whenthechromatidsseparate,whatis each called?
Questions
A. What is the purpose of mitosis?
B. Why is the cell cycle important for organisms?
C. What role does mitosis play in the growth of an embryo?
D. What is one main differencein the mitoticprocesses between plant and animal cells?
E. Ifthecellthathasundergonemitosisoriginallyhadthediploid(2n)numberofchromosomes, then what number of chromosomes will the nuclei in the two new cells have?
F. Can mitosis ever be usedfor reproduction?
G. What parts of plants are most impactedby mitosis?
H. Why might mitosis be easier to study in the tipof the root?
I. Where else in a plant might you finda large amount of mitoticcell division occurring? J. How might the mitoticindex be applied in agriculture?
K. Whatdidyoucomputetobethemitoticindexofthecombinedsixslides(assumethereare
54totalcells)?IncomparisontotheindexesreflectedinFigure8,fromapproximatelywhicharea
of a root was the sample taken?
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CellMembraneTransport
Hands-OnLabs,Inc.Version42-0034-00-01
Lab Report Assistant Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables thatshouldbeaddressedinaformallabreport.Theintentistofacilitatestudents’writingoflab reports by providing this informationin an editable filewhich can be sent to an instructor.
Exercise1:DiffusionthroughanArtificialMembrane
Observations
Data Table 1: Changes During Dialysis | ||
Color | Before Dialysis | AfterDialysis |
Solutionin cup 1 |
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Solutionin dialysis tubing |
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Data Table 2: Benedict’s Reagent Results | ||
Color of Solution | Before Heating | AfterHeating |
IKI solutionin cup 1 |
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Solutionin dialysis bag in cup 2 |
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Distilledwater |
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Questions
A. What is the purpose of this exercise? What is being tested?
B. What color change did you observe in the dialysis tubing? What does that change indicate? C. Was there a color change in the water surrounding the tubing? If so, explain.
D. WhatcolorchangedidyouobserveinthewatercontainingtheIKIafterheating?Whatdoes that change indicate?
E. What does the Benedict’s reagent detect? F. What does the IKI solutiondetect?
G. In what way is a cell membrane similar to the model cell made of dialysis tubing? H. Is the transport mechanism in the model cell passive or active?Why?
Exercise 2: Diffusionat differenttemperatures
Observations
Data Table 3: Diffusionof KMnO4at Various Temperatures and Times | ||
Temperature inside cup | Colorof Water | |
0min | 5min | |
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Questions
I. How does temperature affectthe rate of diffusion?
J. State a general hypothesis to cover how temperature affectsrate of diffusion
Exercise 3: Tonicity and Diffusion
Observations
Data Table 4: Potato Dimensions. | |||
Potato |
BeforeOsmosis (L× W)cm |
AfterOsmosis (L× W)cm | Tonicity Hypertonic, Isotonic, or Hypotonic |
Distilledwater |
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10% Sodium Chloride |
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Questions
A. Whatistheconditionofeachpotatostripaftersoakinginthetesttubesforanhour?Which one is limp and which one is crisp?
B. Howwouldyou explainthedifferenceintheconditionsofthepotatostripsusingtheconcept of tonicity?
C. What was the tonicity of the fresh water solutionwith respect to the potato cells? D. What was the tonicity of the salt water solutionwith respect to the potato cells?
E. How do the changes in the conditionsof the potato strips relate to the wiltingof plants?
F. How does keeping vegetables cool slow them from wilting?
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Photosynthesis &Respiration
Hands-OnLabs,Inc.
Version42-0102-00-01
Lab Report Assistant
Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables thatshouldbeaddressedinaformallabreport.Theintentistofacilitatestudents’writingoflab reports by providing this informationin an editable filewhich can be sent to an instructor.
Observations
Data Table 1: PhotosyntheticRates | |
Rate | Distance(mm) |
Solutionstartingmeasurement |
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Net photosynthesis |
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Respiration |
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Gross photosynthesis (net photosynthesis + respiration) |
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Rate of photosynthesis (gross photosynthesis / 10 min) |
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Bag mass g + 1.5 g NaHCO3 = Final mass (g) | |
Data Table 2: Respiration Results | ||||
Experimental Conditions | Startingdistance (mm) | Final distance (mm) | Change in movement (mm) | Time (min) |
WithElodea |
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WithoutElodea |
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Questions
Exercise 1:PhotosynthesisPartI
A. What molecules did the plant produce when exposed to light? B. What resources were consumed to produce the molecule?
C. What part did light play in the reaction?
D. What molecule was consumed by the plant while it was in the dark? E. What molecule was produced by the plant while it was in the dark? F. Where did the molecules go that were produced in the dark?
G. What criticalservice do plants provide humans?
H. Would animal life on Earth be possible without plants?
I. Would any life on Earth be possible without sunlight?
Exercise 2:PhotosynthesisPartII
A. WhenyoublewthroughthestrawintotheBromothymolbluesolution,whatgasdidyouadd? What color change did you observe in the solutionand why?
B. Whatcolorchangeoccurredafteryouplacedtheplantintotherespirometerandturnedon the light?
C. What was happening when the solutionin the horizontal tubing started to move? D. How long did it take the color change to occur afterthe solutionstarted to move? E. Why did the color change?
F. Whenyourepeatedtheexperimentwithoutaplantintherespirometer,whathappenedand why?
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Plant Genetics
Hands-On Labs, Inc.
Version42-0104-00-01
Lab Report Assistant
Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables thatshouldbeaddressedinaformallabreport.Theintentistofacilitatestudents’writingoflab reports by providing this informationin an editable filewhich can be sent to an instructor.
Observations
Data Table 1: Punnettsquare.
Data Table 2: Seedling data. | |||
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Seedling Color |
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Green | Yellow | Total | |
Numberof Seedlings |
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Percentageof Total |
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Data Table 3: Frequency calculations. | ||
Frequency | Expected Values |
Actual Values |
Green |
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Yellow |
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Total |
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Data Table 4: Corn kernel data. | ||
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Number of Kernels | Kernel Percentage (Divide count by total, then multiplyby 100) |
Kernel Coloration |
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Purple |
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Yellow |
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Total |
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KernelTexture |
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Smooth |
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Wrinkled |
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Total |
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Data Table 5: Punnet square for dihybrid cross.
Data Table 6: Data from corn kernels. | ||
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Number Counted |
Ratio:Number counted / total |
Purple and smooth |
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Purple and wrinkled |
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Yellowand smooth |
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Yellowand wrinkled |
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TOTAL |
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Exercise 1: Observing a Monohybrid Cross
Questions
A. What are the predicted ratiosof the phenotypes in the plants?
B. What was the total number of seeds that germinated?
C. What are the actual ratiosof the phenotypes displayed in the tobacco plants? D. Explain why it may be important to collect data from a larger population.
E. Ifallyellowseedlingswereremovedfromthepopulation,wouldthenextgenerationstillhave a chance at displaying the yellow allele? Explain.
F. Wouldacrossbetweenahomozygousandaheterozygousparentshowthesameratios?
Explain.
Exercise 2: Dihybrid Crossing with Corn
Questions
A. What are the probable phenotypes of the corn ear parents with regard to coloration?
B. What are the probable texture phenotypes of the corn parents?
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