Microbial Metabolism
Aseptic Technique & Culturing Microbes
Cynthia Alonzo, M.S. Version 42-0239-00-01
Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Questions
A. What is the difference between a bactericidal and bacteriostatic agent? What is the difference between sterilization and disinfection?
B. List five microbial killing methods, how they work, and what they are used for.
C. What is a pure culture? Why is it important to work with a pure culture?
D. What is aseptic technique? Why is it so critical?
Aseptic Technique & Culturing Microbes
Experiment
103
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E. Describe three common forms of growth that you are likely to see in a broth culture.
F. What is the difference between an aerobe and an anaerobe?
G. Describe the difference between facultative and obligate.
H. Which two types of media did you use in this experiment? Why did you need two types of media instead of only one?
I. Describe your microscopic observations of the cultures.
J. Define the following terms:
Psychrophile:
Mesophile:
Thermophile:
Hyperthermophile:
K. Which type of organisms did you use in this experiment?