Biology Lab need done
image1.emf UMUC Biology 102/103
Lab 4: Enzymes
INSTRUCTIONS:
· On your own and without assistance, complete this Lab 4 Answer Form electronically and submit it via the Assignments Folder by the date listed on your Course Schedule (under Syllabus).
· To conduct your laboratory exercises, use the Laboratory Manual that is available in the WebTycho classroom (Reserved Reading or provided by your instructor) or at the eScience Labs Student Portal. Laboratory exercises on your CD may not be updated.
· Save your Lab4AnswerForm in the following format: LastName_Lab4 (e.g., Smith_Lab4). .
· You should submit your document in a Word (.doc or .docx) or Rich Text Format (.rtf) for best compatibility.
Experiment 1: Effect of Enzyme Concentration
Table 1: Effect of Enzyme Concentration on the Production of Gas
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Tube |
Amount of yeast |
Balloon circumference (cm) After 1 minute |
Balloon circumference (cm) After 3 minutes |
Balloon circumference (cm) After 5 minutes |
Final Circumference (cm) |
Time Required to Complete |
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1 |
0.05 g |
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2 |
0.005 g |
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3 |
0.0005 g |
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Questions
1. What is the enzyme in this experiment? What is the substrate?
2. Did you notice a difference in the rate of reaction in the tubes with different concentrations of enzymes? Why or why not?
3. What was the effect of using less enzyme on your experiment?
4. Do you expect more enzyme activity if the substrate concentration is increased or decreased? Draw a graph to illustrate this relationship.
5. Hydrogen peroxide is toxic to cells, yet is a common byproduct of the reactions that occur inside the body. How cant his compound be changed to become non-toxic (Hint: Look at the chemical formula of hydrogen peroxide)?
Experiment 2: Effect of Temperature on Enzyme Activity
Table 2: Effect of Temperature on the Production of Gas
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Tube |
Initial Temp. (°C) |
Final Temp. (°C) |
Circumference (cm) After 1 minute |
Circumference (cm) After 3 minutes |
Circumference (cm) After 5 minutes |
Final Circumference (cm) |
Time Required to Complete |
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Refrigerator |
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Room Temperature |
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Hot Water (~85 °C) |
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Boiling Water (~100 °C) |
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Questions
1. What is the enzyme in this experiment? What is the substrate?
2. How does temperature affect enzyme function?
3. Do plants and animals have an enzyme that breaks down hydrogen peroxide? How could you test this?
4. How did the boiling water affect the overall reaction?
5. How can enzyme activity be increased?
6. Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?
7. Draw a graph using balloon diameter vs. temperature. What is the correlation?
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