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I need help with identifying species? 

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JaninaLee-Lab2-1UbiquityofMicroorganisms.docx

Janina Lee Lab 2-1 4-13-2020

Bacillus – large, white colonies. Bacteria.

Sphingomonas – bright yellow colonies. Bacteria.

Staphylococcus – small, white colonies. Bacteria.

Environments – Door Knob (left) / Nail (right)

Bacillus – large, white colony. Bacteria.

Staphylococcus – small, white colonies. Bacteria.

Environments – Mouth (left) / Phone (right)

Bacillus – large, white colonies. Bacteria.

Staphylococcus – small, white colony. Bacteria.

Sphingomonas – bright yellow colonies. Bacteria.

Environments – Phone (left) / Pit (right)

Sphingomonas – bright yellow colonies. Bacteria.

Zygomycetes – black reproductive spores. Fungus. Fuzzy growth.

Bacillus – large, white colonies. Bacteria.

Staphylococcus – small, white colonies. Bacteria.

Environments – Laptop (left) / Cell Phone (right)

Sphingomonas – bright yellow colonies. Bacteria.

Streptomyces – white wrinkled colonies. Bacteria.

Bacillus – large, white colonies. Bacteria.

Environments – Elevator (left) / Trash (right)

Streptomyces – white wrinkled colony. Bacteria.

Sphingomonas – bright yellow colony. Bacteria.

Bacillus – large, white colonies. Bacteria.

Staphylococcus – small, white colonies. Bacteria.

Environments – Back of ear (left) / Lash line (right)

Bacillus – large, white colonies. Bacteria.

Streptomyces – white wrinkled colony. Bacteria.

Staphylococcus – small, white colonies. Bacteria.

Sphingomonas – bright yellow colonies. Bacteria.

Environments – Men’s Toilet (left) / Right Cheek (right)

Staphylococcus – small, white colonies. Bacteria.

Environments – Right Tonsil (left) / Left Tonsil (right)

Question #2: Why do you think the specific types of exposure (air, hair, tabletop, etc.) were chosen for this exercise?

The environments were chosen because they typically have lots of different microbes living on them.

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GroupLabPowerpoint-DianeZetina.pptx

Ubiquity of Microbes

MCRO BIO 233 IK

Diane Zetina

Handprint

Bacillus mycoides (Bacteria) – spiral, webbed pattern. 

Micrococcus luteus (Bacteria) – Bright yellow colonies. 

Streptomyces (Bacteria) - wrinkled colonies, white.

Tonsils

Streptococcus (Bacteria) - Tiny, clear colonies, found in mouth and throat.

Cellphone/Armpit

Staphylococcus (Bacteria) - Small, white colonies.

Bacillus (Bacteria) - Large, white colonies.

Garden Soil

Penicillium (Fungi) - Blue-green in color.

Zygomycetes (Fungi) - Fuzzy growth, black reproductive spores.

Rhodotorula (Fungi) - Found in soil, orange-red color. 

Bathroom floor/Bathroom faucet

Rhodotorula (Fungi) - Slimy, orange-red color, commonly found in water environments.

Streptomyces (Bacteria) - Clear/White, wrinkled colonies.

Miso and Kimchi

Bacillus (Bacteria) - Large, white colonies, live on plants and in soil. 

Streptomyces (Bacteria) - White, wrinkled colonies, abundant in soil. 

Rhodotorula (Fungi) - Slimy, orange-red color, found in soil. 

Laptop and Cellphone

Staphylococcus (Bacteria) - small, creamy white colonies, can be found in things we commonly touch. 

Zygomycetes (Fungi) - Fuzzy, black reproductive spores.

Bacillus (Bacteria) - Large, white colonies

Doorknob and Under Fingernail

Micrococcus luteus (Bacteria) - Bright, yellow colonies, lives on our skin. 

Staphylococcus (Bacteria) - Small, creamy-white colonies, lives on skin. 

Questions

 Which environment/s had the most microbial growth? Why do you think that is? The garden soil is the environment that had the most microbial growth. I think it's because there's so many microbes being mixed with each other that will end up helping the plants grow and stay alive. 

Which environment/s had the least microbial growth? Why do you think that is? I'd say that most of the agar plates had a microbial growth but if I had to choose to have the least microbial growth it would be the doorknob and cell phone. The doorknob might have not had many microbes because it could be cleaned often same thing with the cell phone. 

Did any of the results surprise you? Why? Even though I know there's a bunch of microbes in garden soil I did not expect to see those results. I didn't think the agar plate would be filled with so many things. Another result that surprised me was the handprint. What surprised me was that there was a Bacillus mycoides growth since it's usually found in soil. The person could've had plants and been touching the soil. 

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UbiquityofmicrobessectionJL1.docx

Ubiquity of microbes

Jacob Scarpetta

Lab Section JL

April 2022

A close-up of a petri dish Description automatically generated with medium confidence

Micrococcus luteus, tiny yellow colonies

This looked like a Bacillus bacterium, beige/white coloring

This is the most interesting growth among the various plates (in my unprofessional opinion). I googled around looking for a similar looking colony, but was unsuccessful

A white plate with blue writing on it Description automatically generated with low confidence

Could possibly be of the Penicillium variety, color is not blue/green so I am unsure

Rhizopus stolonifer, black bread mold (in this case black shoe mold)

Hard to define, but based on the color this could be Rhodotorula

Possibly Bacillus myocoides, bacterial strain that has spiral like growth

No growth! This is my groups plate; we swabbed an antimicrobial phone case that has clearly lived up to its claim!

This could be another colony of Bacillus, but it is a very distinct white, rather than a “clear white”

A picture containing text Description automatically generated

Micrococcus luteus

(large and slightly deformed) yellow coloring

Streptomyces, I love this colony! It looks like coral – very unusual shape/color

Bacillus, slimy looking white/grey colony

Micrococcus luteus, yellow bacterial colonies

Possibly Bacillus mycoides, looks like it is spiraling

This one is interesting! It looks as if two strains are growing atop each other, of the Bacillus family

Micrococcus luteus, pungent yellow bacterial colonies

A picture containing text, person Description automatically generated

Staphylococcus, teeny tiny white bacterial colonies

Another ethereal looking colony! I really enjoy these, but am unsure if it should be considered Bacillus mycoides

A picture containing person, hand, watch, gauge Description automatically generated

Micrococcus luteus, great example of the yellow bacterial colonies

Bacillus, ghostly white bacterial colony

Could be Bacillus mycoides, considering the “running edges”

This looks similar to the interesting colony in my first photo, but I think it may have eaten too much food… still unidentified

A picture containing indoor Description automatically generated

Micrococcus luteus, bright yellow bacterial colonies

Staphylococcus, tiny white bacterial colonies

A picture containing person, hand, cheese, tableware Description automatically generated

I mention this area because the entire plate looks cloudy – could this be growth? I wonder if it’s a bacteria that is inhibiting the fungus from growing

Rhizopus stolonifera, black mold!

This looks like a Bacillus bacteria

I really want to consider this Bacillus mycoides, but I think it may be another species

A hand holding a bottle Description automatically generated with low confidence

Micrococcus luteus, yellow colonies

This may be another example of the fungus Rhodotorula

Lab Questions

1. Which environment/s had the most microbial growth? Why do you think that is?

Lillian tested her shoe and, unsurprisingly, that had by far the most growth. The plate looked like it was decomposing, it was very hard to distinguish between different strains due to the large amount of growth.

2. Which environment/s had the least microbial growth? Why do you think that is?

Within my group, Lelanie tested her screen protector which was marketed as antimicrobial. Surprisingly enough, the side of the plate that was swabbed with from a sample swab of that screen protector had no growth! It could be because of antimicrobial properties of the screen protector but I am unsure of what those may be – or if that is even that case.

4. Did any of the results surprise you? Why?

At first glance, the sample from my shoe shocked me that there was so much growth as I tend to wipe my shoes off, but as I thought about it, I do not go through any major disinfection processes, so my results make sense. Also, after seeing Lilian’s results I was much happier with mine

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Ubiquityofmicrobespresentation.pptx
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