LAB

BIOS256 OL, Week 4 Lab

Name:

OL Lab 4: Renal Physiology: Find the mode of action of a diuretic drug

Learning Objectives:

· Understand the morphological relationships between the kidney tubules and the circulatory system

· Analyze experimental data for calculating glomerular filtration rate

· Set up and perform a perfusion of renal tubules, including the calculation of liquid absorption rates

· Learn about the epithelial transport mechanism in the kidney tubules

· Use experimental data to assess the mode of action of a diuretic drug

Kidneys play a critical role in the filtration and reabsorption of essential substances that are required for the body. In this simulation, you will explore the anatomy of the kidney through 3D holograms and identify the physiological roles in maintaining homeostasis as well as the factors that influence urine regulation.

In this simulation, you will use the kidney from a dissected rat to study the mode of action of a new diuretic drug and learn the reabsorption process in the renal tubules. You will learn how to calculate the glomerular filtration rate or GFR and learn the hormones that influence urine output. At the end of the simulation, you should be able to correlate the relationship between renal and circulatory systems in controlling blood pressure.

Part 1: Complete Labster- Renal Physiology: Find the mode of action of a diuretic drug

Part 2: Report and Reflection

Purpose: Describe in your own words and in complete sentences, the purpose of this experiment.

Observations: List 2 observations you have made in this simulation.

Answer all the questions below:

1. In the simulation, why was the ascending limb of the nephron selected to study the effect of the diuretic drug? (2 points)

2. What is renal perfusion and how does it affect blood pressure? (2 points)

3. Compare and contrast the cortical and Juxtamedullary nephrons. (2 points)

4. What are the hormones that regulate urine output? (2 points)

5. What is countercurrent multiplication in a nephron and how does it affect the urine concentration? (2 points)

6. Reflection: Reflect on at least 2 key concepts you have learned from this simulation.

How can you apply it to human health and diseases?

Grading Rubric: 

Activity	Deliverable	Points
Part 1	Complete simulation	15
Part 2	Complete lab report and answer questions  · Purpose (1 point)  · Observation (2 points) · Questions (10 points)  · Reflection (2 points)	15
Total	Complete all lab activities	30